data_SASDBA7_MAIN
#
_sas_result.id                            425
_sas_result.SASBDB_code                   SASDBA7
_sas_result.experimental_MW               86.700
_sas_result.experimental_MW_error         .
_sas_result.MW_standard                   130.380
_sas_result.MW_standard_error             1.290
_sas_result.MW_Porod                      66.585
_sas_result.MW_Porod_error                .
_sas_result.I0_from_PR                    733.0000000000
_sas_result.I0_from_PR_error              16.3300000000
_sas_result.I0_from_Guinier               707.9000000000
_sas_result.I0_from_Guinier_error         7.0400000000
_sas_result.Rg_from_PR                    3.780
_sas_result.Rg_from_PR_error              0.140
_sas_result.Rg_from_Guinier               3.400
_sas_result.Rg_from_Guinier_error         0.077
_sas_result.D_max                         16.40
_sas_result.Porod_volume                  113.20
_sas_result.Porod_volume_error            .
_sas_result.estimated_volume              .
_sas_result.estimated_volume_error        .
_sas_result.estimated_volume_method       .
_sas_result.Guinier_point_min             1
_sas_result.Guinier_point_max             39
_sas_result.type_of_curve                 single_conc
_sas_result.standard                      .
_sas_result.Dmax_error                    0.50
_sas_result.comments                      'We have attempted to characterize this novel complex using SAXS. This complex shows prominent concentration dependency. Due to presence of DNA as part of this complex, "280nm UV absorbance"-based concentration estimations have been largely compromised, producing un-interpretable forward scattering upon concentration normalization. Thus we have relied upon the "concentration-independent" SAXS-MoW to estimate the MW of the scatterer at physiologically relevant micromolar concentrations. MW estimated using qmax=0.2431 (<=8/Rg) suggests this novel complex to be 86.7kDa, comparable to theoretical value of 85.5 kDa.'
#
_sas_scan.title                     "Human NEI like DNA glycosylase 1 (NEIL1) bound to"
_sas_scan.detc_id                   20
_sas_scan.id                        425
_sas_scan.measurement_date          2016-01-20
_sas_scan.storage_temperature       4.0
_sas_scan.cell_temperature          10.0
_sas_scan.exposure_time             0.200
_sas_scan.number_of_frames          24
_sas_scan.unit                      1/A
_sas_scan.qmin                      0.0164
_sas_scan.qmax                      0.5285
_sas_scan.sample_id                 382
_sas_scan.result_id                 425
_sas_scan.beam_id                   21
#
_sas_beam.id                         21
_sas_beam.instrument_name            "Advanced Light Source (ALS) 12.3.1 (SIBYLS)"
_sas_beam.instrument_city            "Berkeley, CA"
_sas_beam.instrument_country         USA
_sas_beam.shape                      .
_sas_beam.type_of_source             "X-ray synchrotron"
_sas_beam.radiation_wavelength       0.112700
_sas_beam.dist_spec_to_detc          1.40000
#
_sas_detc.id              20
_sas_detc.name            "Pilatus3 X 2M"
_sas_detc.type            .
_sas_detc.pixsize_x       172.000
#
_sas_sample.id                           382
_sas_sample.name                         "Human NEI like DNA glycosylase 1 (NEIL1) bound to Proliferating Cell Nuclear Antigen (PCNA) and DNA"
_sas_sample.ext_coefficient              .
_sas_sample.contrast                     .
_sas_sample.specific_vol                 .
_sas_sample.purity_method                .
_sas_sample.dry_vol                      .
_sas_sample.sample_MW                    .
_sas_sample.specimen_concentration       0.60-2.80
_sas_sample.concentration_method         .
loop_
_sas_sample.entity_id                    
453
454
455
#
_sas_buffer.id                  228
_sas_buffer.name                "25mM HEPES 100mM NaCl 1mM DTT"
_sas_buffer.concentration       .
_sas_buffer.unit                .
_sas_buffer.pK                  .
_sas_buffer.pH                  7.500
_sas_buffer.comment             .
_sas_buffer.composition         .
_sas_buffer.sample_id           382
#
loop_
_entity.id                             
_entity.type                           
_entity.pdbx_description               
_entity.formula_weight                 
_entity.pdbx_number_of_molecules       
453 protein "Endonuclease 8-like 1"              44.722 1
454 DNA     dsDNA                                 2.424 1
455 protein "Proliferating cell nuclear antigen" 29.669 1
#
loop_
_entity_name_com.entity_id       
_entity_name_com.name            
453 .
454 .
455 .
#
loop_
_entity_poly.entity_id                      
_entity_poly.pdbx_seq_one_letter_code       
453 MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSASLEHHHHHH
454 
;GCGTCCAGTCTACC
CGCAGGTCCAGATGG
;

455 MHHHHHHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFACICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
#
loop_
_entity_src_gen.entity_id                  
_entity_src_gen.gene_src_common_name       
453 "Homo sapiens"
454 .             
455 "Homo sapiens"
#
loop_
_struct_ref.id              
_struct_ref.entity_id       
_struct_ref.db_name         
_struct_ref.db_code         
_struct_ref.details         
1 453 UniProt Q96FI4 http://www.uniprot.org/uniprot/Q96FI4
2 454 UniProt .      http://www.uniprot.org/uniprot/P12004