data_SASDFA8_MAIN # _sas_result.id 1481 _sas_result.SASBDB_code SASDFA8 _sas_result.experimental_MW 42.800 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 47.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 1716.6000000000 _sas_result.I0_from_Guinier_error 8.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 4.610 _sas_result.Rg_from_Guinier_error 0.600 _sas_result.D_max 22.00 _sas_result.Porod_volume 76.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 27 _sas_result.Guinier_point_max 129 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1481 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7904 _sas_scan.sample_id 1442 _sas_scan.result_id 1481 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1442 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.60 _sas_sample.concentration_method . _sas_sample.entity_id 1738 # _sas_buffer.id 898 _sas_buffer.name "20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1442 # _entity.id 1738 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta - mutant" _entity.formula_weight 20.405 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1738 _entity_name_com.name "rpoE KE mutant" # _entity_poly.entity_id 1738 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVEAEEEEAEEAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1738 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1738 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 1.267276E+04 3.014621E+03 1481 2 1.546980E-02 1.283891E+04 1.927009E+03 1481 3 1.756010E-02 7.796381E+03 1.204523E+03 1481 4 1.965030E-02 4.585914E+03 8.116553E+02 1481 5 2.174050E-02 3.092879E+03 6.616996E+02 1481 6 2.383070E-02 3.438054E+03 5.179739E+02 1481 7 2.592090E-02 2.784825E+03 4.621887E+02 1481 8 2.801120E-02 2.843502E+03 3.556853E+02 1481 9 3.010140E-02 2.584280E+03 3.105607E+02 1481 10 3.219160E-02 1.959420E+03 2.687735E+02 1481 11 3.428180E-02 2.152798E+03 2.268357E+02 1481 12 3.637210E-02 2.144268E+03 2.077203E+02 1481 13 3.846230E-02 1.896696E+03 1.716920E+02 1481 14 4.055250E-02 2.335911E+03 1.591720E+02 1481 15 4.264270E-02 2.111486E+03 1.441891E+02 1481 16 4.473290E-02 2.000693E+03 1.275625E+02 1481 17 4.682320E-02 1.822872E+03 1.221726E+02 1481 18 4.891340E-02 1.825000E+03 1.127587E+02 1481 19 5.100360E-02 1.740735E+03 1.115849E+02 1481 20 5.309380E-02 1.639829E+03 1.021998E+02 1481 21 5.518410E-02 1.727774E+03 9.507537E+01 1481 22 5.727430E-02 1.556809E+03 9.349191E+01 1481 23 5.936450E-02 1.849949E+03 8.683350E+01 1481 24 6.145470E-02 1.533051E+03 8.828549E+01 1481 25 6.354490E-02 1.740089E+03 7.840432E+01 1481 26 6.563520E-02 1.868661E+03 7.823397E+01 1481 27 6.772540E-02 1.718257E+03 7.510961E+01 1481 28 6.981560E-02 1.656542E+03 7.357891E+01 1481 29 7.190580E-02 1.730035E+03 6.854350E+01 1481 30 7.399610E-02 1.812455E+03 6.682981E+01 1481 31 7.608630E-02 1.532214E+03 6.623051E+01 1481 32 7.817650E-02 1.708159E+03 6.357506E+01 1481 33 8.026670E-02 1.702276E+03 6.224027E+01 1481 34 8.235690E-02 1.724191E+03 6.006463E+01 1481 35 8.444720E-02 1.725927E+03 5.881245E+01 1481 36 8.653740E-02 1.626461E+03 5.830175E+01 1481 37 8.862760E-02 1.619890E+03 5.513195E+01 1481 38 9.071780E-02 1.615881E+03 5.400747E+01 1481 39 9.280810E-02 1.645282E+03 5.320732E+01 1481 40 9.489830E-02 1.657684E+03 5.412953E+01 1481 41 9.698850E-02 1.604729E+03 5.346930E+01 1481 42 9.907870E-02 1.607568E+03 4.983700E+01 1481 43 1.011690E-01 1.644513E+03 5.094035E+01 1481 44 1.032590E-01 1.572469E+03 4.880428E+01 1481 45 1.053490E-01 1.591561E+03 4.969883E+01 1481 46 1.074400E-01 1.677594E+03 4.771770E+01 1481 47 1.095300E-01 1.610997E+03 4.674222E+01 1481 48 1.116200E-01 1.530995E+03 4.814747E+01 1481 49 1.137100E-01 1.528644E+03 4.581424E+01 1481 50 1.158000E-01 1.582866E+03 4.515704E+01 1481 51 1.178910E-01 1.506443E+03 4.403938E+01 1481 52 1.199810E-01 1.588967E+03 4.436918E+01 1481 53 1.220710E-01 1.622683E+03 4.522206E+01 1481 54 1.241610E-01 1.513404E+03 4.210591E+01 1481 55 1.262520E-01 1.563112E+03 4.284447E+01 1481 56 1.283420E-01 1.494292E+03 4.178171E+01 1481 57 1.304320E-01 1.525724E+03 4.182669E+01 1481 58 1.325220E-01 1.578937E+03 4.246436E+01 1481 59 1.346120E-01 1.577677E+03 4.100650E+01 1481 60 1.367030E-01 1.460442E+03 4.040689E+01 1481 61 1.387930E-01 1.520992E+03 3.972525E+01 1481 62 1.408830E-01 1.455748E+03 3.972070E+01 1481 63 1.429730E-01 1.457675E+03 3.827686E+01 1481 64 1.450640E-01 1.511537E+03 3.887882E+01 1481 65 1.471540E-01 1.479431E+03 3.913630E+01 1481 66 1.492440E-01 1.450196E+03 3.744765E+01 1481 67 1.513340E-01 1.455879E+03 3.741186E+01 1481 68 1.534240E-01 1.419565E+03 3.683792E+01 1481 69 1.555150E-01 1.416248E+03 3.696587E+01 1481 70 1.576050E-01 1.324189E+03 3.641401E+01 1481 71 1.596950E-01 1.406352E+03 3.580454E+01 1481 72 1.617850E-01 1.372291E+03 3.562284E+01 1481 73 1.638760E-01 1.403623E+03 3.507582E+01 1481 74 1.659660E-01 1.415554E+03 3.575010E+01 1481 75 1.680560E-01 1.433398E+03 3.545193E+01 1481 76 1.701460E-01 1.398838E+03 3.446440E+01 1481 77 1.722360E-01 1.368904E+03 3.505162E+01 1481 78 1.743270E-01 1.371749E+03 3.379572E+01 1481 79 1.764170E-01 1.333758E+03 3.394602E+01 1481 80 1.785070E-01 1.307206E+03 3.278355E+01 1481 81 1.805970E-01 1.353814E+03 3.355390E+01 1481 82 1.826880E-01 1.310991E+03 3.349865E+01 1481 83 1.847780E-01 1.304535E+03 3.260342E+01 1481 84 1.868680E-01 1.332394E+03 3.261237E+01 1481 85 1.889580E-01 1.333264E+03 3.208131E+01 1481 86 1.910480E-01 1.370698E+03 3.225146E+01 1481 87 1.931390E-01 1.342069E+03 3.251262E+01 1481 88 1.952290E-01 1.307780E+03 3.122530E+01 1481 89 1.973190E-01 1.285379E+03 3.196035E+01 1481 90 1.994090E-01 1.303285E+03 3.106740E+01 1481 91 2.015000E-01 1.271563E+03 3.128987E+01 1481 92 2.035900E-01 1.254314E+03 3.057897E+01 1481 93 2.056800E-01 1.262138E+03 3.006322E+01 1481 94 2.077700E-01 1.217896E+03 3.118373E+01 1481 95 2.098600E-01 1.258256E+03 2.968902E+01 1481 96 2.119510E-01 1.236344E+03 3.058527E+01 1481 97 2.140410E-01 1.200216E+03 2.912838E+01 1481 98 2.161310E-01 1.226857E+03 2.937942E+01 1481 99 2.182210E-01 1.208525E+03 2.990050E+01 1481 100 2.203120E-01 1.199456E+03 2.905113E+01 1481 101 2.224020E-01 1.283193E+03 2.917291E+01 1481 102 2.244920E-01 1.168191E+03 2.888791E+01 1481 103 2.265820E-01 1.217849E+03 2.861510E+01 1481 104 2.286720E-01 1.168915E+03 2.836705E+01 1481 105 2.307630E-01 1.186525E+03 2.825930E+01 1481 106 2.328530E-01 1.141333E+03 2.812632E+01 1481 107 2.349430E-01 1.136554E+03 2.791623E+01 1481 108 2.370330E-01 1.168461E+03 2.813998E+01 1481 109 2.391240E-01 1.095870E+03 2.774565E+01 1481 110 2.412140E-01 1.110889E+03 2.701689E+01 1481 111 2.433040E-01 1.105901E+03 2.755864E+01 1481 112 2.453940E-01 1.118081E+03 2.704096E+01 1481 113 2.474840E-01 1.108302E+03 2.739326E+01 1481 114 2.495750E-01 1.118873E+03 2.649365E+01 1481 115 2.516650E-01 1.076988E+03 2.675535E+01 1481 116 2.537550E-01 1.121782E+03 2.663908E+01 1481 117 2.558450E-01 1.100382E+03 2.672162E+01 1481 118 2.579360E-01 1.059330E+03 2.612118E+01 1481 119 2.600260E-01 1.074844E+03 2.620228E+01 1481 120 2.621160E-01 1.096017E+03 2.648040E+01 1481 121 2.642060E-01 1.053639E+03 2.573300E+01 1481 122 2.662960E-01 1.038965E+03 2.564171E+01 1481 123 2.683870E-01 1.018814E+03 2.556063E+01 1481 124 2.704770E-01 1.019424E+03 2.499926E+01 1481 125 2.725670E-01 1.050207E+03 2.568757E+01 1481 126 2.746570E-01 1.054454E+03 2.523461E+01 1481 127 2.767480E-01 9.745732E+02 2.464431E+01 1481 128 2.788380E-01 1.030900E+03 2.506412E+01 1481 129 2.809280E-01 1.013171E+03 2.480622E+01 1481 130 2.830180E-01 1.020623E+03 2.497259E+01 1481 131 2.851080E-01 1.025224E+03 2.432721E+01 1481 132 2.871990E-01 9.944918E+02 2.476649E+01 1481 133 2.892890E-01 9.628132E+02 2.427506E+01 1481 134 2.913790E-01 9.795844E+02 2.415265E+01 1481 135 2.934690E-01 9.203759E+02 2.397077E+01 1481 136 2.955600E-01 1.002714E+03 2.373523E+01 1481 137 2.976500E-01 9.668125E+02 2.433774E+01 1481 138 2.997400E-01 9.354444E+02 2.379593E+01 1481 139 3.018300E-01 9.019712E+02 2.340437E+01 1481 140 3.039200E-01 9.597023E+02 2.383596E+01 1481 141 3.060110E-01 9.136307E+02 2.314610E+01 1481 142 3.081010E-01 9.529191E+02 2.384299E+01 1481 143 3.101910E-01 9.737829E+02 2.303594E+01 1481 144 3.122810E-01 8.666856E+02 2.278369E+01 1481 145 3.143720E-01 9.219996E+02 2.315463E+01 1481 146 3.164620E-01 8.913533E+02 2.280115E+01 1481 147 3.185520E-01 8.963739E+02 2.306505E+01 1481 148 3.206420E-01 8.873825E+02 2.208726E+01 1481 149 3.227320E-01 8.809770E+02 2.276378E+01 1481 150 3.248230E-01 8.589883E+02 2.242921E+01 1481 151 3.269130E-01 8.912066E+02 2.256998E+01 1481 152 3.290030E-01 7.775887E+02 2.328872E+01 1481 153 3.310930E-01 8.431774E+02 2.171416E+01 1481 154 3.331840E-01 8.591144E+02 2.226916E+01 1481 155 3.352740E-01 8.554934E+02 2.217933E+01 1481 156 3.373640E-01 8.346802E+02 2.178151E+01 1481 157 3.394540E-01 7.992934E+02 2.190081E+01 1481 158 3.415440E-01 8.183420E+02 2.153496E+01 1481 159 3.436350E-01 8.151658E+02 2.178518E+01 1481 160 3.457250E-01 8.013658E+02 2.131287E+01 1481 161 3.478150E-01 8.265856E+02 2.165336E+01 1481 162 3.499050E-01 7.924152E+02 2.242521E+01 1481 163 3.519960E-01 8.084070E+02 2.296872E+01 1481 164 3.540860E-01 8.091802E+02 2.352363E+01 1481 165 3.561760E-01 8.038798E+02 2.301159E+01 1481 166 3.582660E-01 7.976479E+02 2.379390E+01 1481 167 3.603560E-01 7.680097E+02 2.384558E+01 1481 168 3.624470E-01 7.715062E+02 2.431055E+01 1481 169 3.645370E-01 7.950685E+02 2.437346E+01 1481 170 3.666270E-01 7.936949E+02 2.427394E+01 1481 171 3.687170E-01 7.756887E+02 2.494918E+01 1481 172 3.708080E-01 7.749296E+02 2.446599E+01 1481 173 3.728980E-01 7.553879E+02 2.433717E+01 1481 174 3.749880E-01 7.662054E+02 2.432748E+01 1481 175 3.770780E-01 7.711494E+02 2.471823E+01 1481 176 3.791680E-01 7.195062E+02 2.436491E+01 1481 177 3.812590E-01 7.062062E+02 2.354690E+01 1481 178 3.833490E-01 7.019358E+02 2.444535E+01 1481 179 3.854390E-01 6.879066E+02 2.420777E+01 1481 180 3.875290E-01 7.275763E+02 2.427985E+01 1481 181 3.896200E-01 7.559004E+02 2.477791E+01 1481 182 3.917100E-01 7.310004E+02 2.383951E+01 1481 183 3.938000E-01 6.850977E+02 2.402592E+01 1481 184 3.958900E-01 7.359447E+02 2.273343E+01 1481 185 3.979800E-01 7.020665E+02 2.256118E+01 1481 186 4.000710E-01 6.516599E+02 2.230288E+01 1481 187 4.021610E-01 6.667868E+02 2.177284E+01 1481 188 4.042510E-01 6.753782E+02 2.219656E+01 1481 189 4.063410E-01 7.034230E+02 2.145755E+01 1481 190 4.084320E-01 6.414747E+02 2.149477E+01 1481 191 4.105220E-01 6.761249E+02 2.127119E+01 1481 192 4.126120E-01 6.903047E+02 2.102811E+01 1481 193 4.147020E-01 7.037716E+02 2.164453E+01 1481 194 4.167920E-01 6.451821E+02 2.063769E+01 1481 195 4.188830E-01 6.848268E+02 2.078561E+01 1481 196 4.209730E-01 6.472661E+02 2.053370E+01 1481 197 4.230630E-01 6.465767E+02 2.054532E+01 1481 198 4.251530E-01 6.475035E+02 2.049378E+01 1481 199 4.272440E-01 6.244377E+02 2.013172E+01 1481 200 4.293340E-01 6.786732E+02 2.042539E+01 1481 201 4.314240E-01 6.281798E+02 1.990345E+01 1481 202 4.335140E-01 6.173409E+02 2.008537E+01 1481 203 4.356040E-01 6.076794E+02 1.958972E+01 1481 204 4.376950E-01 5.771128E+02 1.974777E+01 1481 205 4.397850E-01 6.317992E+02 1.998693E+01 1481 206 4.418750E-01 5.831374E+02 1.934398E+01 1481 207 4.439650E-01 6.314658E+02 1.954919E+01 1481 208 4.460560E-01 6.213786E+02 1.942844E+01 1481 209 4.481460E-01 5.857315E+02 1.931012E+01 1481 210 4.502360E-01 6.008498E+02 1.973079E+01 1481 211 4.523260E-01 6.009560E+02 1.872075E+01 1481 212 4.544160E-01 5.797953E+02 1.911137E+01 1481 213 4.565070E-01 5.924342E+02 1.908651E+01 1481 214 4.585970E-01 6.268222E+02 1.921562E+01 1481 215 4.606870E-01 5.967654E+02 1.883185E+01 1481 216 4.627770E-01 6.040553E+02 1.888250E+01 1481 217 4.648680E-01 5.419463E+02 1.871797E+01 1481 218 4.669580E-01 5.690564E+02 1.861678E+01 1481 219 4.690480E-01 5.965611E+02 1.900081E+01 1481 220 4.711380E-01 5.581984E+02 1.827124E+01 1481 221 4.732280E-01 5.581584E+02 1.853034E+01 1481 222 4.753190E-01 5.787802E+02 1.859637E+01 1481 223 4.774090E-01 5.876471E+02 1.844064E+01 1481 224 4.794990E-01 5.750506E+02 1.819316E+01 1481 225 4.815890E-01 5.303533E+02 1.795758E+01 1481 226 4.836800E-01 5.763696E+02 1.842707E+01 1481 227 4.857700E-01 5.505790E+02 1.804274E+01 1481 228 4.878600E-01 5.289607E+02 1.786561E+01 1481 229 4.899500E-01 5.452358E+02 1.811530E+01 1481 230 4.920400E-01 5.358132E+02 1.784761E+01 1481 231 4.941310E-01 5.753008E+02 1.810174E+01 1481 232 4.962210E-01 5.530241E+02 1.772711E+01 1481 233 4.983110E-01 5.202183E+02 1.739245E+01 1481 234 5.004010E-01 5.353366E+02 1.764079E+01 1481 235 5.024920E-01 4.990790E+02 1.732638E+01 1481 236 5.045820E-01 4.884370E+02 1.741948E+01 1481 237 5.066720E-01 4.971665E+02 1.714523E+01 1481 238 5.087620E-01 4.859074E+02 1.720371E+01 1481 239 5.108520E-01 5.234782E+02 1.727574E+01 1481 240 5.129430E-01 5.288268E+02 1.715193E+01 1481 241 5.150330E-01 5.285580E+02 1.722834E+01 1481 242 5.171230E-01 5.139743E+02 1.687849E+01 1481 243 5.192130E-01 5.183957E+02 1.710579E+01 1481 244 5.213040E-01 5.111634E+02 1.718446E+01 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