data_SASDGB3_MAIN # _sas_result.id 1578 _sas_result.SASBDB_code SASDGB3 _sas_result.experimental_MW 65.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier . _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR 2.000 _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier . _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min . _sas_result.Guinier_point_max . _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "This curve is part of a pH series. Increasing the pH removes aggregation presumably due to protein surface charge changes. The SAXS data for this entry is measured from aggregated material and therefore the Rg and I(0) parameters do not apply." # _sas_scan.title "DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)" _sas_scan.detc_id 20 _sas_scan.id 1578 _sas_scan.measurement_date 2018-11-02 _sas_scan.storage_temperature 10.0 _sas_scan.cell_temperature 10.0 _sas_scan.exposure_time 3.000 _sas_scan.number_of_frames 300 _sas_scan.unit 1/A _sas_scan.qmin 0.0081 _sas_scan.qmax 0.3910 _sas_scan.sample_id 1539 _sas_scan.result_id 1578 _sas_scan.beam_id 21 # _sas_beam.id 21 _sas_beam.instrument_name "Advanced Light Source (ALS) 12.3.1 (SIBYLS)" _sas_beam.instrument_city "Berkeley, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.103000 _sas_beam.dist_spec_to_detc 1.50000 # _sas_detc.id 20 _sas_detc.name "Pilatus3 X 2M" _sas_detc.type . _sas_detc.pixsize_x 172.000 # _sas_sample.id 1539 _sas_sample.name "DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration . _sas_sample.concentration_method . loop_ _sas_sample.entity_id 1622 1623 1632 # _sas_buffer.id 850 _sas_buffer.name "10 mM sodium acetate, 50 mM NaCl" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 4.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1539 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 1622 DNA "80bp_DNA Forward" 24.849 1 1623 DNA "80bp_DNA Reverse" 24.612 1 1632 protein "DNA-binding protein HU-alpha, E34K" 9.534 2 # loop_ _entity_name_com.entity_id _entity_name_com.name 1622 . 1623 . 1632 . # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 1622 AATGAGGTAACAACGAAAGCAGATGATAGCTGCTTATCAATTTGTTGCAAACAAGTAGCCGCGCCCAATGAGGTAACAAT 1623 TTACTCCATTGTTGCTTTCGTCTACTATCGACGAATAGTTAAACAACGTTTGTTCATCGGCGCGGGTTACTCCATTGTTA 1632 ;MNKTQLIDVIAEKAELSKTQAKAALESTLAAITKSLKEGDAVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFVSGKALKDAVK ; # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 1622 "Escherichia coli" 1623 "Escherichia coli K-12" 1632 "Escherichia coli K-12" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code 1 1622 UniProt . 2 1623 UniProt . 3 1632 UniProt P0ACF0 _struct_ref.details http://www.uniprot.org/uniprot/P0ACF0 # _pdbx_contact_author.id 240 _pdbx_contact_author.name_first Soumya _pdbx_contact_author.name_last "G Remesh" _pdbx_contact_author.role . _pdbx_contact_author.address_1 . _pdbx_contact_author.address_2 "Lawrence Berkeley National laboratory" _pdbx_contact_author.address_3 "Berkeley, CA, USA" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 8.083000E-03 8.366440E+02 6.926430E+00 1578 2 8.640000E-03 6.800030E+02 8.951960E+00 1578 3 9.198000E-03 5.447210E+02 5.791200E+00 1578 4 9.755000E-03 4.277050E+02 5.089360E+00 1578 5 1.031300E-02 3.509670E+02 4.322420E+00 1578 6 1.087000E-02 3.445990E+02 1.972060E+01 1578 7 1.142800E-02 2.982790E+02 1.418020E+01 1578 8 1.198500E-02 2.499050E+02 1.034510E+01 1578 9 1.254300E-02 2.105360E+02 8.476920E+00 1578 10 1.310000E-02 1.742090E+02 7.593660E+00 1578 11 1.365800E-02 1.469510E+02 6.508210E+00 1578 12 1.421500E-02 1.286950E+02 6.599480E+00 1578 13 1.477200E-02 1.085170E+02 5.408820E+00 1578 14 1.533000E-02 9.740840E+01 5.427360E+00 1578 15 1.588700E-02 8.601910E+01 5.051480E+00 1578 16 1.644500E-02 7.649830E+01 4.476840E+00 1578 17 1.700200E-02 6.771960E+01 3.923810E+00 1578 18 1.756000E-02 6.047490E+01 3.875160E+00 1578 19 1.811700E-02 5.413850E+01 3.387760E+00 1578 20 1.867500E-02 4.837570E+01 3.172350E+00 1578 21 1.923200E-02 4.393450E+01 2.789690E+00 1578 22 1.978900E-02 4.021850E+01 2.304000E+00 1578 23 2.034700E-02 3.847950E+01 1.808380E+00 1578 24 2.090400E-02 3.503470E+01 1.578580E+00 1578 25 2.146200E-02 3.219410E+01 1.420600E+00 1578 26 2.201900E-02 3.031280E+01 1.152640E+00 1578 27 2.257700E-02 2.842330E+01 9.801490E-01 1578 28 2.313400E-02 2.650950E+01 8.556790E-01 1578 29 2.369200E-02 2.417570E+01 7.852030E-01 1578 30 2.424900E-02 2.325040E+01 6.626110E-01 1578 31 2.480700E-02 2.193170E+01 5.799730E-01 1578 32 2.536400E-02 2.034120E+01 5.331620E-01 1578 33 2.592100E-02 1.907350E+01 4.941880E-01 1578 34 2.647900E-02 1.909560E+01 4.358890E-01 1578 35 2.703600E-02 1.815470E+01 3.960170E-01 1578 36 2.759400E-02 1.720060E+01 3.715690E-01 1578 37 2.815100E-02 1.597520E+01 3.499410E-01 1578 38 2.870900E-02 1.508780E+01 3.324350E-01 1578 39 2.926600E-02 1.436180E+01 3.122560E-01 1578 40 2.982400E-02 1.381330E+01 3.076150E-01 1578 41 3.038100E-02 1.349970E+01 2.902390E-01 1578 42 3.093800E-02 1.301910E+01 2.767950E-01 1578 43 3.149600E-02 1.249220E+01 2.779490E-01 1578 44 3.205300E-02 1.218290E+01 2.720410E-01 1578 45 3.261100E-02 1.216910E+01 2.586480E-01 1578 46 3.316800E-02 1.176970E+01 2.607710E-01 1578 47 3.372600E-02 1.115370E+01 2.580640E-01 1578 48 3.428300E-02 1.100150E+01 2.555790E-01 1578 49 3.484100E-02 1.129390E+01 2.487440E-01 1578 50 3.539800E-02 1.040180E+01 2.478390E-01 1578 51 3.595500E-02 1.050970E+01 2.465330E-01 1578 52 3.651300E-02 1.026130E+01 2.423630E-01 1578 53 3.707000E-02 9.626460E+00 2.316970E-01 1578 54 3.762800E-02 9.453080E+00 2.310820E-01 1578 55 3.818500E-02 9.638440E+00 2.329880E-01 1578 56 3.874300E-02 9.550680E+00 2.258410E-01 1578 57 3.930000E-02 9.179240E+00 2.235100E-01 1578 58 3.985800E-02 8.483370E+00 2.199990E-01 1578 59 4.041500E-02 9.105250E+00 2.228740E-01 1578 60 4.097300E-02 8.506930E+00 2.199220E-01 1578 61 4.153000E-02 8.446710E+00 2.143280E-01 1578 62 4.208700E-02 8.444020E+00 2.086550E-01 1578 63 4.264500E-02 8.234700E+00 2.123280E-01 1578 64 4.320200E-02 8.296500E+00 2.036400E-01 1578 65 4.376000E-02 7.901240E+00 2.026390E-01 1578 66 4.431700E-02 8.230970E+00 2.050680E-01 1578 67 4.487500E-02 8.105960E+00 1.960360E-01 1578 68 4.543200E-02 7.497560E+00 1.980750E-01 1578 69 4.599000E-02 7.380450E+00 1.952130E-01 1578 70 4.654700E-02 7.213790E+00 1.990550E-01 1578 71 4.710400E-02 7.207720E+00 1.866590E-01 1578 72 4.766200E-02 7.274540E+00 1.864380E-01 1578 73 4.821900E-02 7.009720E+00 1.858840E-01 1578 74 4.877700E-02 7.317520E+00 1.864840E-01 1578 75 4.933400E-02 7.145760E+00 1.810880E-01 1578 76 4.989200E-02 6.806140E+00 1.777560E-01 1578 77 5.044900E-02 6.837730E+00 1.810030E-01 1578 78 5.100700E-02 6.539960E+00 1.809820E-01 1578 79 5.156400E-02 6.471190E+00 1.774500E-01 1578 80 5.212100E-02 6.266530E+00 1.736860E-01 1578 81 5.267900E-02 6.391580E+00 1.770290E-01 1578 82 5.323600E-02 6.571270E+00 1.712160E-01 1578 83 5.379400E-02 6.358140E+00 1.729740E-01 1578 84 5.435100E-02 6.137270E+00 1.683760E-01 1578 85 5.490900E-02 6.007290E+00 1.692750E-01 1578 86 5.546600E-02 6.184220E+00 1.667210E-01 1578 87 5.602400E-02 6.042840E+00 1.662990E-01 1578 88 5.658100E-02 6.030240E+00 1.659880E-01 1578 89 5.713800E-02 5.638870E+00 1.628390E-01 1578 90 5.769600E-02 5.718060E+00 1.610220E-01 1578 91 5.825300E-02 5.699100E+00 1.630840E-01 1578 92 5.881100E-02 5.546010E+00 1.621700E-01 1578 93 5.936800E-02 5.517820E+00 1.594210E-01 1578 94 5.992600E-02 5.330980E+00 1.537690E-01 1578 95 6.048300E-02 5.242520E+00 1.572210E-01 1578 96 6.104100E-02 5.229110E+00 1.576230E-01 1578 97 6.159800E-02 5.352420E+00 1.547930E-01 1578 98 6.215500E-02 5.130080E+00 1.487170E-01 1578 99 6.271300E-02 5.261080E+00 1.528090E-01 1578 100 6.327000E-02 5.083340E+00 1.540150E-01 1578 101 6.382800E-02 4.815050E+00 1.485840E-01 1578 102 6.438500E-02 4.978810E+00 1.495690E-01 1578 103 6.494300E-02 4.817970E+00 1.483560E-01 1578 104 6.550000E-02 4.534760E+00 1.474470E-01 1578 105 6.605700E-02 4.611780E+00 1.474100E-01 1578 106 6.661500E-02 4.968790E+00 1.448520E-01 1578 107 6.717200E-02 4.420750E+00 1.440300E-01 1578 108 6.773000E-02 4.491020E+00 1.434200E-01 1578 109 6.828700E-02 4.601930E+00 1.413700E-01 1578 110 6.884500E-02 4.520290E+00 1.399630E-01 1578 111 6.940200E-02 4.272480E+00 1.406620E-01 1578 112 6.996000E-02 4.250290E+00 1.381690E-01 1578 113 7.051700E-02 4.299620E+00 1.399970E-01 1578 114 7.107400E-02 4.282010E+00 1.417260E-01 1578 115 7.163200E-02 4.353070E+00 1.422900E-01 1578 116 7.218900E-02 4.234610E+00 1.349570E-01 1578 117 7.274700E-02 4.207060E+00 1.378540E-01 1578 118 7.330400E-02 4.017250E+00 1.389800E-01 1578 119 7.386200E-02 4.077050E+00 1.403340E-01 1578 120 7.441900E-02 3.946540E+00 1.348950E-01 1578 121 7.497700E-02 4.038890E+00 1.362900E-01 1578 122 7.553400E-02 3.906640E+00 1.395260E-01 1578 123 7.609100E-02 3.888780E+00 1.372540E-01 1578 124 7.664900E-02 3.860850E+00 1.335310E-01 1578 125 7.720600E-02 3.673700E+00 1.320120E-01 1578 126 7.776400E-02 3.860220E+00 1.338210E-01 1578 127 7.832100E-02 3.716850E+00 1.321990E-01 1578 128 7.887900E-02 3.383960E+00 1.320530E-01 1578 129 7.943600E-02 3.704290E+00 1.331460E-01 1578 130 7.999300E-02 3.685510E+00 1.330520E-01 1578 131 8.055100E-02 3.441640E+00 1.296610E-01 1578 132 8.110800E-02 3.591270E+00 1.294380E-01 1578 133 8.166600E-02 3.512630E+00 1.290700E-01 1578 134 8.222300E-02 3.369200E+00 1.288980E-01 1578 135 8.278100E-02 3.418700E+00 1.274130E-01 1578 136 8.333800E-02 3.433120E+00 1.256710E-01 1578 137 8.389500E-02 3.484370E+00 1.276790E-01 1578 138 8.445300E-02 3.162730E+00 1.250100E-01 1578 139 8.501000E-02 3.211570E+00 1.250060E-01 1578 140 8.556800E-02 3.066310E+00 1.236400E-01 1578 141 8.612500E-02 3.185430E+00 1.260270E-01 1578 142 8.668300E-02 3.283370E+00 1.224480E-01 1578 143 8.724000E-02 3.072700E+00 1.206950E-01 1578 144 8.779800E-02 3.117060E+00 1.232530E-01 1578 145 8.835500E-02 2.957180E+00 1.229340E-01 1578 146 8.891200E-02 3.204790E+00 1.206340E-01 1578 147 8.947000E-02 3.149260E+00 1.197310E-01 1578 148 9.002700E-02 2.991390E+00 1.199590E-01 1578 149 9.058500E-02 3.167560E+00 1.204090E-01 1578 150 9.114200E-02 2.829120E+00 1.181540E-01 1578 151 9.170000E-02 2.721630E+00 1.183430E-01 1578 152 9.225700E-02 3.013760E+00 1.213810E-01 1578 153 9.281400E-02 2.782610E+00 1.202980E-01 1578 154 9.337200E-02 2.638750E+00 1.225630E-01 1578 155 9.392900E-02 2.813040E+00 1.256970E-01 1578 156 9.448700E-02 2.579770E+00 1.280240E-01 1578 157 9.504400E-02 2.684270E+00 1.275950E-01 1578 158 9.560200E-02 2.708360E+00 1.268370E-01 1578 159 9.615900E-02 2.567370E+00 1.299730E-01 1578 160 9.671600E-02 2.604570E+00 1.308730E-01 1578 161 9.727400E-02 2.642480E+00 1.323080E-01 1578 162 9.783100E-02 2.684430E+00 1.307330E-01 1578 163 9.838900E-02 2.697470E+00 1.324670E-01 1578 164 9.894600E-02 2.646960E+00 1.308250E-01 1578 165 9.950400E-02 2.531270E+00 1.285920E-01 1578 166 1.000610E-01 2.438910E+00 1.283600E-01 1578 167 1.006180E-01 2.752880E+00 1.258680E-01 1578 168 1.011760E-01 2.407840E+00 1.270190E-01 1578 169 1.017330E-01 2.554580E+00 1.218320E-01 1578 170 1.022910E-01 2.439920E+00 1.229080E-01 1578 171 1.028480E-01 2.395670E+00 1.222330E-01 1578 172 1.034060E-01 2.124660E+00 1.200010E-01 1578 173 1.039630E-01 2.112710E+00 1.192420E-01 1578 174 1.045200E-01 2.195460E+00 1.179650E-01 1578 175 1.050780E-01 2.103890E+00 1.169240E-01 1578 176 1.056350E-01 2.245030E+00 1.151160E-01 1578 177 1.061930E-01 2.303140E+00 1.167300E-01 1578 178 1.067500E-01 2.252600E+00 1.143830E-01 1578 179 1.073080E-01 2.256360E+00 1.145530E-01 1578 180 1.078650E-01 2.138770E+00 1.134210E-01 1578 181 1.084220E-01 2.095730E+00 1.134490E-01 1578 182 1.089800E-01 2.029870E+00 1.126120E-01 1578 183 1.095370E-01 1.978800E+00 1.103970E-01 1578 184 1.100950E-01 2.221470E+00 1.106070E-01 1578 185 1.106520E-01 2.039620E+00 1.109720E-01 1578 186 1.112100E-01 2.233580E+00 1.114240E-01 1578 187 1.117670E-01 2.290780E+00 1.080350E-01 1578 188 1.123240E-01 2.095770E+00 1.071640E-01 1578 189 1.128820E-01 2.254540E+00 1.090280E-01 1578 190 1.134390E-01 1.869070E+00 1.074790E-01 1578 191 1.139970E-01 1.960180E+00 1.064220E-01 1578 192 1.145540E-01 1.892550E+00 1.058660E-01 1578 193 1.151110E-01 2.059910E+00 1.067410E-01 1578 194 1.156690E-01 2.043010E+00 1.052630E-01 1578 195 1.162260E-01 2.014290E+00 1.054580E-01 1578 196 1.167840E-01 2.042110E+00 1.042320E-01 1578 197 1.173410E-01 1.936570E+00 1.041520E-01 1578 198 1.178990E-01 1.692020E+00 1.023240E-01 1578 199 1.184560E-01 1.791820E+00 1.030830E-01 1578 200 1.190130E-01 1.907390E+00 1.023840E-01 1578 201 1.195710E-01 1.929640E+00 1.033020E-01 1578 202 1.201280E-01 1.977600E+00 1.012600E-01 1578 203 1.206860E-01 1.884240E+00 1.009080E-01 1578 204 1.212430E-01 1.783580E+00 1.012740E-01 1578 205 1.218000E-01 1.805230E+00 1.005900E-01 1578 206 1.223580E-01 1.678170E+00 9.954470E-02 1578 207 1.229150E-01 1.663490E+00 9.945750E-02 1578 208 1.234730E-01 1.812790E+00 1.004050E-01 1578 209 1.240300E-01 1.747680E+00 9.864350E-02 1578 210 1.245880E-01 1.760350E+00 9.826670E-02 1578 211 1.251450E-01 1.785820E+00 9.876120E-02 1578 212 1.257020E-01 1.919190E+00 9.918840E-02 1578 213 1.262600E-01 1.751350E+00 9.729150E-02 1578 214 1.268170E-01 1.787140E+00 9.604030E-02 1578 215 1.273750E-01 1.739130E+00 9.634150E-02 1578 216 1.279320E-01 1.723720E+00 9.696640E-02 1578 217 1.284890E-01 1.640450E+00 9.601100E-02 1578 218 1.290470E-01 1.637050E+00 9.554050E-02 1578 219 1.296040E-01 1.489460E+00 9.589090E-02 1578 220 1.301620E-01 1.827360E+00 9.475480E-02 1578 221 1.307190E-01 1.527230E+00 9.477390E-02 1578 222 1.312770E-01 1.744360E+00 9.679110E-02 1578 223 1.318340E-01 1.599030E+00 1.205090E-01 1578 224 1.323910E-01 1.510760E+00 1.872290E-01 1578 225 1.329490E-01 1.298610E+00 1.890150E-01 1578 226 1.335060E-01 1.544910E+00 2.183780E-01 1578 227 1.340640E-01 1.529190E+00 2.400330E-01 1578 228 1.346210E-01 1.396210E+00 3.467530E-01 1578 229 1.351780E-01 1.295290E+00 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