{ "code": "SASDCB7", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.", "publication": { "title": "Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.", "author_list": "Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G", "journal": "Structure", "doi": "10.1016/j.str.2017.10.001", "pmid": "29107484", "published_date": "2017 Dec 5" }, "status": "released", "submitted_date": "2017-09-01", "released_date": "2017-10-31" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCB7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCB7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCB7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCB7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCB7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCB7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCB7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "DHH subfamily 1 protein", "short_name": "SpnPde2", "sequence": "SMEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHFPEKTIKAVGFDEPTLT\r\nWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIKIDHHPNDDVYGDLSWVDT\r\nSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFLYPSTTARTLRLAAYLREHNF\r\nDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVILSQEVLKQYNVTDAETAAIVGA\r\nPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIAKEHDGGGHPLASGANSYSLEENEI\r\nIYQKLKNLLKN", "organism": "Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)", "uniprot_code": "A0A0H2ZNP2", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHFPEKTIKAVGFDEPTLT\nWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIKIDHHPNDDVYGDLSWVDT\nSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFLYPSTTARTLRLAAYLREHNF\nDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVILSQEVLKQYNVTDAETAAIVGA\nPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIAKEHDGGGHPLASGANSYSLEENEI\nIYQKLKNLLKN", "mw": 35.053, "total_mw": 70.106, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20mM Tris, 200 mM NaCl, 5%(v/v) glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Streptococcus pneumoniae phosphodiesterase Pde2", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Munich, Germany", "full_name": "Ludwig-Maximilians-Universität München", "webpage": "https://www.en.uni-muenchen.de" } ], "contributor_name": "Gregor", "contributor_surname": "Witte", "orcid": "https://orcid.org/0000-0003-0313-0962" } ], "concentration_method": null, "concentration_unit": null, "date": "2015-06-23", "storage_temperature": null, "cell_temperature": 20.0, "exposure_time": 0.045, "number_of_frames": 39, "wavelength": 0.123987, "sample_detector_distance": 3.0, "concentration_min": null, "concentration_max": 7.0, "sample_volume": null, "flow_rate": null, "s_min": 0.027, "s_max": 4.808, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Streptococcus pneumoniae phosphodiesterase Pde2 in 20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH 7.5, were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123987 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 7.00 mg/ml was measured at 20°C. 39 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 57.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 67.0, "porod_mw_error": null, "pddf_i0": 21410.0, "pddf_i0_error": 13.4, "guinier_i0": 21234.0, "guinier_i0_error": 19.57, "pddf_rg": 2.72, "pddf_rg_error": 0.148, "guinier_rg": 2.69, "guinier_rg_error": 0.23, "pddf_dmax": 7.74, "pddf_dmax_error": null, "porod_volume": 86.56, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 14, "guinier_point_last": 146, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCB7_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }