data_SASDFB8_MAIN # _sas_result.id 1482 _sas_result.SASBDB_code SASDFB8 _sas_result.experimental_MW 28.900 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 49.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 4417.0000000000 _sas_result.I0_from_Guinier_error 22.0000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 4.490 _sas_result.Rg_from_Guinier_error 0.820 _sas_result.D_max 24.00 _sas_result.Porod_volume 78.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 30 _sas_result.Guinier_point_max 133 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1482 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7925 _sas_scan.sample_id 1443 _sas_scan.result_id 1482 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1443 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.60 _sas_sample.concentration_method . _sas_sample.entity_id 1738 # _sas_buffer.id 899 _sas_buffer.name "20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1443 # _entity.id 1738 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta - mutant" _entity.formula_weight 20.405 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1738 _entity_name_com.name "rpoE KE mutant" # _entity_poly.entity_id 1738 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVEAEEEEAEEAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1738 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1738 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 8.117000E+03 8.055430E+03 1482 2 1.546980E-02 1.080000E+03 5.135189E+03 1482 3 1.756010E-02 5.956500E+03 3.212064E+03 1482 4 1.965030E-02 2.726400E+03 2.165684E+03 1482 5 2.174050E-02 3.632500E+03 1.768092E+03 1482 6 2.383070E-02 3.538100E+03 1.384391E+03 1482 7 2.592090E-02 5.512700E+03 1.234402E+03 1482 8 2.801120E-02 6.273900E+03 9.533675E+02 1482 9 3.010140E-02 6.219000E+03 8.314471E+02 1482 10 3.219160E-02 5.683070E+03 7.205136E+02 1482 11 3.428180E-02 5.910110E+03 6.069117E+02 1482 12 3.637210E-02 4.899130E+03 5.602637E+02 1482 13 3.846230E-02 4.954340E+03 4.608372E+02 1482 14 4.055250E-02 4.870260E+03 4.250210E+02 1482 15 4.264270E-02 4.204210E+03 3.860513E+02 1482 16 4.473290E-02 5.183520E+03 3.423056E+02 1482 17 4.682320E-02 4.148290E+03 3.279750E+02 1482 18 4.891340E-02 4.179570E+03 3.009526E+02 1482 19 5.100360E-02 4.487530E+03 3.003149E+02 1482 20 5.309380E-02 4.043990E+03 2.742194E+02 1482 21 5.518410E-02 4.093420E+03 2.569398E+02 1482 22 5.727430E-02 4.188130E+03 2.514246E+02 1482 23 5.936450E-02 4.315210E+03 2.336186E+02 1482 24 6.145470E-02 4.863730E+03 2.387584E+02 1482 25 6.354490E-02 4.352869E+03 2.107646E+02 1482 26 6.563520E-02 4.082039E+03 2.083799E+02 1482 27 6.772540E-02 4.128511E+03 2.024562E+02 1482 28 6.981560E-02 3.998047E+03 1.968823E+02 1482 29 7.190580E-02 4.830586E+03 1.874545E+02 1482 30 7.399610E-02 4.418975E+03 1.792911E+02 1482 31 7.608630E-02 4.161441E+03 1.790347E+02 1482 32 7.817650E-02 4.035599E+03 1.712497E+02 1482 33 8.026670E-02 4.262134E+03 1.684398E+02 1482 34 8.235690E-02 4.497541E+03 1.614118E+02 1482 35 8.444720E-02 4.261199E+03 1.572845E+02 1482 36 8.653740E-02 4.202696E+03 1.570489E+02 1482 37 8.862760E-02 4.288355E+03 1.500040E+02 1482 38 9.071780E-02 4.183871E+03 1.487178E+02 1482 39 9.280810E-02 4.304876E+03 1.435243E+02 1482 40 9.489830E-02 4.228713E+03 1.476494E+02 1482 41 9.698850E-02 4.161302E+03 1.456819E+02 1482 42 9.907870E-02 4.255558E+03 1.349563E+02 1482 43 1.011690E-01 4.382353E+03 1.378162E+02 1482 44 1.032590E-01 4.009128E+03 1.326041E+02 1482 45 1.053490E-01 4.161749E+03 1.343249E+02 1482 46 1.074400E-01 3.999782E+03 1.287113E+02 1482 47 1.095300E-01 4.332804E+03 1.283382E+02 1482 48 1.116200E-01 4.190514E+03 1.325688E+02 1482 49 1.137100E-01 4.165997E+03 1.246625E+02 1482 50 1.158000E-01 4.212112E+03 1.238235E+02 1482 51 1.178910E-01 4.065698E+03 1.210683E+02 1482 52 1.199810E-01 3.976285E+03 1.186498E+02 1482 53 1.220710E-01 3.891707E+03 1.224182E+02 1482 54 1.241610E-01 3.903099E+03 1.141979E+02 1482 55 1.262520E-01 4.077476E+03 1.163189E+02 1482 56 1.283420E-01 3.953623E+03 1.151853E+02 1482 57 1.304320E-01 4.075530E+03 1.141387E+02 1482 58 1.325220E-01 4.027152E+03 1.146743E+02 1482 59 1.346120E-01 3.978993E+03 1.109160E+02 1482 60 1.367030E-01 3.842479E+03 1.100894E+02 1482 61 1.387930E-01 3.818529E+03 1.069660E+02 1482 62 1.408830E-01 3.845815E+03 1.084595E+02 1482 63 1.429730E-01 3.979030E+03 1.050996E+02 1482 64 1.450640E-01 3.926943E+03 1.058180E+02 1482 65 1.471540E-01 3.685667E+03 1.059571E+02 1482 66 1.492440E-01 3.723684E+03 1.022875E+02 1482 67 1.513340E-01 3.903693E+03 1.026057E+02 1482 68 1.534240E-01 3.641890E+03 9.976756E+01 1482 69 1.555150E-01 3.575406E+03 1.000087E+02 1482 70 1.576050E-01 3.733596E+03 1.015465E+02 1482 71 1.596950E-01 3.556829E+03 9.736866E+01 1482 72 1.617850E-01 3.551137E+03 9.757605E+01 1482 73 1.638760E-01 3.540912E+03 9.558922E+01 1482 74 1.659660E-01 3.594187E+03 9.736634E+01 1482 75 1.680560E-01 3.589721E+03 9.584914E+01 1482 76 1.701460E-01 3.562068E+03 9.308835E+01 1482 77 1.722360E-01 3.658566E+03 9.626945E+01 1482 78 1.743270E-01 3.539561E+03 9.249008E+01 1482 79 1.764170E-01 3.581957E+03 9.310209E+01 1482 80 1.785070E-01 3.453600E+03 8.947033E+01 1482 81 1.805970E-01 3.383896E+03 9.102147E+01 1482 82 1.826880E-01 3.534227E+03 9.234288E+01 1482 83 1.847780E-01 3.613394E+03 9.047170E+01 1482 84 1.868680E-01 3.456311E+03 8.858387E+01 1482 85 1.889580E-01 3.503960E+03 8.784821E+01 1482 86 1.910480E-01 3.486241E+03 8.761494E+01 1482 87 1.931390E-01 3.363288E+03 8.781505E+01 1482 88 1.952290E-01 3.325118E+03 8.483707E+01 1482 89 1.973190E-01 3.385754E+03 8.702835E+01 1482 90 1.994090E-01 3.426510E+03 8.486720E+01 1482 91 2.015000E-01 3.298681E+03 8.605963E+01 1482 92 2.035900E-01 3.319232E+03 8.375036E+01 1482 93 2.056800E-01 3.287383E+03 8.191984E+01 1482 94 2.077700E-01 3.285652E+03 8.563231E+01 1482 95 2.098600E-01 3.287210E+03 8.194063E+01 1482 96 2.119510E-01 3.377395E+03 8.434567E+01 1482 97 2.140410E-01 3.213455E+03 7.989725E+01 1482 98 2.161310E-01 3.014307E+03 7.953287E+01 1482 99 2.182210E-01 3.234809E+03 8.247210E+01 1482 100 2.203120E-01 3.251096E+03 7.954528E+01 1482 101 2.224020E-01 3.101120E+03 7.869440E+01 1482 102 2.244920E-01 3.159753E+03 7.896042E+01 1482 103 2.265820E-01 2.992004E+03 7.792406E+01 1482 104 2.286720E-01 3.127521E+03 7.804754E+01 1482 105 2.307630E-01 2.978022E+03 7.630875E+01 1482 106 2.328530E-01 3.075550E+03 7.726683E+01 1482 107 2.349430E-01 3.019379E+03 7.619362E+01 1482 108 2.370330E-01 3.105008E+03 7.764954E+01 1482 109 2.391240E-01 3.000424E+03 7.567938E+01 1482 110 2.412140E-01 3.025296E+03 7.430699E+01 1482 111 2.433040E-01 2.927184E+03 7.539327E+01 1482 112 2.453940E-01 2.851815E+03 7.399940E+01 1482 113 2.474840E-01 2.861542E+03 7.506096E+01 1482 114 2.495750E-01 2.986692E+03 7.269645E+01 1482 115 2.516650E-01 2.953621E+03 7.367999E+01 1482 116 2.537550E-01 2.900030E+03 7.280269E+01 1482 117 2.558450E-01 2.770648E+03 7.281760E+01 1482 118 2.579360E-01 2.814954E+03 7.191775E+01 1482 119 2.600260E-01 2.749057E+03 7.066264E+01 1482 120 2.621160E-01 2.839979E+03 7.198177E+01 1482 121 2.642060E-01 2.748230E+03 7.052991E+01 1482 122 2.662960E-01 2.692588E+03 6.975506E+01 1482 123 2.683870E-01 2.908735E+03 7.064085E+01 1482 124 2.704770E-01 2.742448E+03 6.860903E+01 1482 125 2.725670E-01 2.689046E+03 7.046432E+01 1482 126 2.746570E-01 2.712255E+03 6.841148E+01 1482 127 2.767480E-01 2.649064E+03 6.759448E+01 1482 128 2.788380E-01 2.568332E+03 6.787393E+01 1482 129 2.809280E-01 2.751939E+03 6.849051E+01 1482 130 2.830180E-01 2.541542E+03 6.772076E+01 1482 131 2.851080E-01 2.566833E+03 6.630873E+01 1482 132 2.871990E-01 2.711789E+03 6.855814E+01 1482 133 2.892890E-01 2.369090E+03 6.551012E+01 1482 134 2.913790E-01 2.556692E+03 6.664426E+01 1482 135 2.934690E-01 2.560390E+03 6.583795E+01 1482 136 2.955600E-01 2.411606E+03 6.438422E+01 1482 137 2.976500E-01 2.524589E+03 6.667028E+01 1482 138 2.997400E-01 2.561156E+03 6.594802E+01 1482 139 3.018300E-01 2.408017E+03 6.414519E+01 1482 140 3.039200E-01 2.444077E+03 6.467555E+01 1482 141 3.060110E-01 2.319898E+03 6.301577E+01 1482 142 3.081010E-01 2.533201E+03 6.564097E+01 1482 143 3.101910E-01 2.344194E+03 6.278874E+01 1482 144 3.122810E-01 2.358482E+03 6.338299E+01 1482 145 3.143720E-01 2.439380E+03 6.343460E+01 1482 146 3.164620E-01 2.291461E+03 6.231223E+01 1482 147 3.185520E-01 2.477299E+03 6.347124E+01 1482 148 3.206420E-01 2.196240E+03 6.036928E+01 1482 149 3.227320E-01 2.327369E+03 6.237982E+01 1482 150 3.248230E-01 2.329257E+03 6.183305E+01 1482 151 3.269130E-01 2.155222E+03 6.097361E+01 1482 152 3.290030E-01 2.524598E+03 6.443268E+01 1482 153 3.310930E-01 2.272602E+03 5.987451E+01 1482 154 3.331840E-01 2.175085E+03 6.087687E+01 1482 155 3.352740E-01 2.256834E+03 6.081697E+01 1482 156 3.373640E-01 2.152272E+03 5.971023E+01 1482 157 3.394540E-01 2.208765E+03 6.008397E+01 1482 158 3.415440E-01 2.220850E+03 5.908012E+01 1482 159 3.436350E-01 2.218983E+03 5.992742E+01 1482 160 3.457250E-01 2.190752E+03 5.849562E+01 1482 161 3.478150E-01 2.166263E+03 5.943054E+01 1482 162 3.499050E-01 2.101033E+03 6.137221E+01 1482 163 3.519960E-01 2.236534E+03 6.376726E+01 1482 164 3.540860E-01 2.032185E+03 6.385025E+01 1482 165 3.561760E-01 2.059424E+03 6.221393E+01 1482 166 3.582660E-01 2.040515E+03 6.520383E+01 1482 167 3.603560E-01 1.914551E+03 6.506071E+01 1482 168 3.624470E-01 1.994680E+03 6.648861E+01 1482 169 3.645370E-01 1.978119E+03 6.622629E+01 1482 170 3.666270E-01 2.062177E+03 6.671931E+01 1482 171 3.687170E-01 2.101811E+03 6.808695E+01 1482 172 3.708080E-01 2.004926E+03 6.728956E+01 1482 173 3.728980E-01 1.898897E+03 6.559265E+01 1482 174 3.749880E-01 1.975235E+03 6.624020E+01 1482 175 3.770780E-01 1.899707E+03 6.668245E+01 1482 176 3.791680E-01 1.963730E+03 6.743176E+01 1482 177 3.812590E-01 1.915753E+03 6.445773E+01 1482 178 3.833490E-01 2.004164E+03 6.789836E+01 1482 179 3.854390E-01 1.951849E+03 6.683306E+01 1482 180 3.875290E-01 1.765615E+03 6.615850E+01 1482 181 3.896200E-01 1.849634E+03 6.773565E+01 1482 182 3.917100E-01 1.960490E+03 6.524903E+01 1482 183 3.938000E-01 1.897875E+03 6.574132E+01 1482 184 3.958900E-01 1.837035E+03 6.212252E+01 1482 185 3.979800E-01 1.917702E+03 6.248612E+01 1482 186 4.000710E-01 1.787422E+03 6.088402E+01 1482 187 4.021610E-01 1.765138E+03 5.978794E+01 1482 188 4.042510E-01 1.725162E+03 6.082372E+01 1482 189 4.063410E-01 1.848785E+03 5.895179E+01 1482 190 4.084320E-01 1.691202E+03 5.874737E+01 1482 191 4.105220E-01 1.813820E+03 5.850881E+01 1482 192 4.126120E-01 1.900117E+03 5.797496E+01 1482 193 4.147020E-01 1.766362E+03 5.892426E+01 1482 194 4.167920E-01 1.826446E+03 5.697040E+01 1482 195 4.188830E-01 1.751619E+03 5.692455E+01 1482 196 4.209730E-01 1.783700E+03 5.637509E+01 1482 197 4.230630E-01 1.763221E+03 5.651314E+01 1482 198 4.251530E-01 1.683747E+03 5.642116E+01 1482 199 4.272440E-01 1.664872E+03 5.489782E+01 1482 200 4.293340E-01 1.618607E+03 5.577490E+01 1482 201 4.314240E-01 1.563916E+03 5.441007E+01 1482 202 4.335140E-01 1.607527E+03 5.510022E+01 1482 203 4.356040E-01 1.594375E+03 5.398212E+01 1482 204 4.376950E-01 1.682330E+03 5.463414E+01 1482 205 4.397850E-01 1.557355E+03 5.461094E+01 1482 206 4.418750E-01 1.613953E+03 5.373965E+01 1482 207 4.439650E-01 1.593804E+03 5.339823E+01 1482 208 4.460560E-01 1.702061E+03 5.378483E+01 1482 209 4.481460E-01 1.613670E+03 5.314805E+01 1482 210 4.502360E-01 1.497349E+03 5.390998E+01 1482 211 4.523260E-01 1.572778E+03 5.193247E+01 1482 212 4.544160E-01 1.689918E+03 5.335281E+01 1482 213 4.565070E-01 1.628939E+03 5.280392E+01 1482 214 4.585970E-01 1.503505E+03 5.234331E+01 1482 215 4.606870E-01 1.423906E+03 5.109655E+01 1482 216 4.627770E-01 1.477606E+03 5.150472E+01 1482 217 4.648680E-01 1.524390E+03 5.170103E+01 1482 218 4.669580E-01 1.480280E+03 5.098863E+01 1482 219 4.690480E-01 1.526427E+03 5.159047E+01 1482 220 4.711380E-01 1.495493E+03 5.015701E+01 1482 221 4.732280E-01 1.367699E+03 5.045169E+01 1482 222 4.753190E-01 1.430046E+03 5.074718E+01 1482 223 4.774090E-01 1.544490E+03 5.058717E+01 1482 224 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