{ "code": "SASDXB9", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase.", "publication": { "title": "A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase.", "author_list": "Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE", "journal": "Nucleic Acids Res", "doi": "10.1093/nar/gkag029", "pmid": "41556341", "published_date": "2026 Jan 14" }, "status": "released", "submitted_date": "2025-09-18", "released_date": "2026-01-23" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXB9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXB9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXB9_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXB9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXB9.sascif", "experiment": { "instrument": { "detector": { "type": "Hybrid Photon Counting (HPC) X-ray detector", "name": "Rigaku HyPix-3000", "resolution": 100.0 }, "name": "Pennsylvania State University", "city": "University Park, PA", "country": "USA", "beamline_name": "Rigaku BioSAXS-2000", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "RNA-directed RNA polymerase nsP4", "short_name": "RdRp nsP4", "sequence": "YIFSSDTGQGHLQQKSVRQTTLPVNIVEEVHEEKCYPPKLDEIKEQLLLKRLQESASTAN\r\nRSRYQSRKVENMKAMIIHRLKEGCRLYLASDTPRVPSYRITYPAPIYSPSINIKLSNPET\r\nAVAVCNEFLARNYPTVASYQVTDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHSYHA\r\nPTIRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTMDSAAFNVECFKKYACNQEYWRE\r\nFASSPIRVTTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTMDMKRDVKVTPGT\r\nKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIATH\r\nFKPGDAVLETDIASFDKSQDDSLALTAMMLLEDLGVDQPILDLIEAAFGEISSCHLPTGT\r\nRFKFGAMMKSGMFLTLFVNTLLNITIASRVLEERLTTSACAAFIGDDNIIHGVVSDALMA\r\nARCATWMNMEVKIIDAVVSVKAPYFCGGFILHDTVTGTACRVADPLKRLFKLGKPLAAGD\r\nEQDEDRRRALADEVTRWQRTGLVTELERAVYSRYEVQGITAVITSMATFASSKENFKKLR\r\nGPVVTLYGGPK", "organism": "O'nyong-nyong virus", "uniprot_code": "O90368", "uniprot_range_first": 1903, "uniprot_range_last": 2513, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MDSVYVDIDADSAFLKALQRAYPMFEVEPKQVTPNDHANARAFSHLAIKLIEQEIDPDST\nILDIGPAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAAGKVTDKNISGKINDLQ\nAVMAVPNMETSTFCLHTDATCKQRGDVAIYQDVYAVHAPTSLYHQAIKGVRVAYWIGFDT\nTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLSEGRRGKLSIMRGKKLKPCDRVL\nFSVGSTLYPESRKLLQSWHLPSVFHLKGKLSFTCRCDTIVSCEGYVVKRVTMSPGIYGKT\nSGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICDQMTGILATEVTPEDAQKLLV\nGLNQRIVVNGRTQRNTNTMKNYLLPIVAQAFSKWAKECRKDMEDEKLLGVRERTLTCCCL\nWAFRKHKTHTVYKRPDTQSIQKVPAEFDSFVIPSLWSSGLSIPLRTRIKWLLSKAPKHEQ\nLPHSGNAEEAAQAEMDAAEEREAELTREAMPPLQATQDDVQVEIDVEQLEDRAGAGIVET\nPRGAIKVTAQPSDRVVGEYLVLTPQAVLRSQKLSLIHALAEQVKTCTHSGRAGRYAVEAY\nDGRVLVPSGYAIPQEDFQSLSESATMVFNEREFVNRKLHHIAMHGPALNTDEESYELVRV\nEKTEHEYVYDVDQKKCCKREEATGLVLVGDLTSPPYHEFAYEGLKIRPACPYKTAVIGVF\nGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEISNDVMRQRKLEISARTVDSLLLNGCNKP\nVEVLYVDEAFACHSGTLLALIAMVRPRQKVVLCGDPKQCGFFNMMQMKVNYNHNICTQVY\nHKSISRRCTLPVTAIVSSLHYESKMRTTNEYNQPIVVDTTGITKPEPGDLVLTCFRGWVK\nQLQIDYRGNEVMTAAASQGLTRKGVYAVRQKVNENPLYASTSEHVNVLLTRTEGKLIWKT\nLSGDPWIKILQNPPKGNFKATIKEWEAEHASIMAGICNHQMAFDTFQNKANVCWAKCLVP\nILDTAGIKLSDRQWSQIVQAFKEDRAYSPEVALNEICTRIYGVDLDSGLFSKPLISVYYA\nDNHWDNRPGGKMFGFNPEVALMLEKKYPFTKGKWNINKQICITTRKVDEFNPETNIIPAN\nRRLPHSLVAEHHTVRGERMEWLVNKINGHHMLLVSGYNLILPTKRVTWVAPLGTRGADYT\nYNLELGLPATLGRYDLVVINIHTPFRIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIR\nAYGYADRTSERVISVLGRKFRSSRALKPQCITSNTEMFFLFSRFDNGRRNFTTHVMNNQL\nNAVYAGLATRAGCAPSYRVKRMDIAKNTEECVVNAANPRGVPGDGVCKAVYRKWPESFRN\nSATPVGTAKTIMCGQYPVIHAVGPNFSNYSEAEGDRELASVYREVAKEVSRLGVSSVAIP\nLLSTGVYSGGKDRLLQSLNHLFTAMDSTDADVVIYCRDKEWEKKITEAISLRSQVELLDD\nHISVDCDIVRVHPDSSLAGRKGYSTVEGALYSYLEGTRFHQTAVDMAEIYTMWPKQTEAN\nEQVCLYALGESIESVRQKCPVDDADASFPPKTVPCLCRYAMTPERVARLRMNHTTSIIVC\nSSFPLPKYKIEGVQKVKCSKALLFDHNVPSRVSPRTYRPADEIIQTPQIPTEACQDAQFV\nQSITDEAVPVPSDLEACDATMDWPSIDIVPTRQRSDSFDSEYSSRSNIQLVTADVHAPMY\nANSLASSGGSVLSLSSEQAQNGIMILPDSEDTDSISRVSTPIAPPRRRLGRTINVTCDER\nEGKILPMASDRLFTAKPYTVALGVSTADITAYPIQAPLGSTQPPALEQITFGDFAEGEID\nNLLTGALTFGDFEPGEVEELTDSEWSTCSDTDEELXLDRAGGYIFSSDTGQGHLQQKSVR\nQTTLPVNIVEEVHEEKCYPPKLDEIKEQLLLKRLQESASTANRSRYQSRKVENMKATIIH\nRLKEGCRLYLASDTPRVPSYRITYPAPVYSPSISIKLNNPETAVAVCNEFLARNYPTVAS\nYQVTDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHSYHAPTIRSAVPSPFQNTLQNV\nLAAATKRNCNVTQMRELPTMDSAVFNVECFKKYACNQEYWREFASSPIRVTTENLTMYVT\nKLKGPKAAALFAKTHNLLPLQEVPMDRFTMDMKRDVKVTPGTKHTEERPKVQVIQAAEPL\nATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIISTHFKPGDAVLETDIASFDKS\nQDDSLALTAMMLLEDLGVDQPILDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFV\nNTLLNITIASRVLEERLTTSACAAFIGDDNIIHGVVSDALMAARCATWMNMEVKIIDAVV\nSEKAPYFCGGFILHDTVTGTSCRVADPLKRLFKLGKPLAAGDEQDEDRRRALADEVTRWQ\nRTGLITELEKAVYSRYEVQGITAVITSMATFASSKENFKKLRGPVVTLYGGPK", "mw": 68.29, "total_mw": 68.29, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Replicates genomic and antigenomic RNA by recognizing replications specific signals. The early replication complex formed by the polyprotein P123 and nsP4 synthesizes minus-strand RNAs." } ], "buffer": { "name": "25 mM HEPES, 1 mM TCEP, 5% glycerol and 500 mM NaCl.", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "O’Nyong-Nyong virus (ONNV) nsP4 full length wild type", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "PSU", "address": "Pennsylvania, USA", "full_name": "Pennsylvania State University (PSU)", "webpage": "https://www.psu.edu/" } ], "contributor_name": "Neela", "contributor_surname": "Yennawar", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2023-06-08", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 600.0, "number_of_frames": 6, "wavelength": 0.154, "sample_detector_distance": 0.4955, "concentration_min": null, "concentration_max": 2.9, "sample_volume": null, "flow_rate": null, "s_min": 0.107, "s_max": 6.135, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 74.3, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 5.56155, "guinier_i0_error": null, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 3.62, "guinier_rg_error": 0.642, "pddf_dmax": null, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 6, "guinier_point_last": 32, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDXB9_datloglog_img.png", "symmetry": null, "last_modified": "2026-01-23T16:11:10.998145+01:00", "bragg_peak": [] }