{ "code": "SASD2C2", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/nm", "project": { "title": "Acetyl-CoA decarbonylase/synthase (ACDS) from Methanosarcina acetivorans", "publication": null, "status": "released", "submitted_date": "2026-07-01", "released_date": "2026-07-29" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASD2C2.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASD2C2.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASD2C2_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2C2_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASD2C2.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 2M", "resolution": 0.172 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "point", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit alpha 2", "short_name": "CdhA", "sequence": ">Q8TJC6\r\nMSKLTTGSFSIEDLESVQITINNIVGAAKEAAEEKAKELGPMGPTAMAGLASYRSWNLLL\r\nLDRYEPVLTPMCDQCCYCTYGPCDLSGNKRGACGIDMAGQTGREFFLRVITGTACHAAHG\r\nRHLLDHVIEVFGEDLPLNLGESNVLTPNVTICTGLSPKTLGECRAPMEYVEEQLTQLLAT\r\nIHAGQESAEIDYDSKALFSGSLDHVGMEVSDIAQVSAYDFPKADPEAPLIEIGMGSIDKS\r\nKPLIVAIGHNVAGVTYIMDYMEENNLTDKMEIAGLCCTAFDMTRYKEADRRAPYAKIVGS\r\nLAKELKVIRSGMPDVIVVDEQCVRGDVLSESQKLKIPVIASNEKIMMGLPDRTDADVDSI\r\nVEEIKSGAIPGCVMLDYDKLGELIPKIAEVMAPIRDAEGITAIPTDEEFKVYIDKCVKCG\r\nECMLACPEELDIPEALEYAAKGSYEYLEALHDVCIGCRRCEQVCKKEIPILNVLEKAAQK\r\nSISEEKGWVRSGRGQASDAEIRKEGLNLVMGTTPGIIAIIGCPNYPAGTKDVYLIAEEFL\r\nKRNYLLAVSGCSAMDIGMFKDEDGKTLYEKYPGTFAGGGLLNTGSCVSNAHISGAAEKVA\r\nGIFAQRTLAGNLAEIADYTLNRVGACGLAWGAYSQKAASIGTGCNIYGIPAVLGPHSSKY\r\nRRALIAKNYDESKWKVYDGRDGSEMTIPPAPEFLLTTAETWQEAIPMMAKACIRPSDNNM\r\nGRSIKLTHWMELSKKYLGVEPEDWWKFVRNEADLPLAKREELLKRLEAEHGWEIDWKRKK\r\nIISGPKIKFDVSAQPTNLKRLCKEA", "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)", "uniprot_code": "Q8TJC6", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MSKLTTGSFSIEDLESVQITINNIVGAAKEAAEEKAKELGPMGPTAMAGLASYRSWNLLL\nLDRYEPVLTPMCDQCCYCTYGPCDLSGNKRGACGIDMAGQTGREFFLRVITGTACHAAHG\nRHLLDHVIEVFGEDLPLNLGESNVLTPNVTICTGLSPKTLGECRAPMEYVEEQLTQLLAT\nIHAGQESAEIDYDSKALFSGSLDHVGMEVSDIAQVSAYDFPKADPEAPLIEIGMGSIDKS\nKPLIVAIGHNVAGVTYIMDYMEENNLTDKMEIAGLCCTAFDMTRYKEADRRAPYAKIVGS\nLAKELKVIRSGMPDVIVVDEQCVRGDVLSESQKLKIPVIASNEKIMMGLPDRTDADVDSI\nVEEIKSGAIPGCVMLDYDKLGELIPKIAEVMAPIRDAEGITAIPTDEEFKVYIDKCVKCG\nECMLACPEELDIPEALEYAAKGSYEYLEALHDVCIGCRRCEQVCKKEIPILNVLEKAAQK\nSISEEKGWVRSGRGQASDAEIRKEGLNLVMGTTPGIIAIIGCPNYPAGTKDVYLIAEEFL\nKRNYLLAVSGCSAMDIGMFKDEDGKTLYEKYPGTFAGGGLLNTGSCVSNAHISGAAEKVA\nGIFAQRTLAGNLAEIADYTLNRVGACGLAWGAYSQKAASIGTGCNIYGIPAVLGPHSSKY\nRRALIAKNYDESKWKVYDGRDGSEMTIPPAPEFLLTTAETWQEAIPMMAKACIRPSDNNM\nGRSIKLTHWMELSKKYLGVEPEDWWKFVRNEADLPLAKREELLKRLEAEHGWEIDWKRKK\nIISGPKIKFDVSAQPTNLKRLCKEA", "mw": 87.954, "total_mw": 703.632, "number_molecules": 8, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit epsilon 2", "short_name": "CdhB", "sequence": ">Q8TJC5\r\nMVDTTKNTKLFTSYGVTTSKTTTPEIAAKLISKAKRPLLVVGTKVLDPELLDRAVKIAQK\r\nANIPIAATGSSMPGFVGKDVDAKYINLHQLGFYVTDPNWPGLDGNGTYDTLIVLGHIKYY\r\nINQVLSGTKNFSTVKAIAIERNYIQNATMSFGNLSKADHYAALDELIDAL", "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)", "uniprot_code": "Q8TJC5", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MVDTTKNTKLFTSYGVTTSKTTTPEIAAKLISKAKRPLLVVGTKVLDPELLDRAVKIAQK\nANIPIAATGSSMPGFVGKDVDAKYINLHQLGFYVTDPNWPGLDGNGTYDTLIVLGHIKYY\nINQVLSGTKNFSTVKAIAIERNYIQNATMSFGNLSKADHYAALDELIDAL", "mw": 18.466, "total_mw": 147.728, "number_molecules": 8, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit beta 2", "short_name": "CdhC", "sequence": ">Q8TJC4\r\nMAEFPFEISPMFEGERVRKEGMFVELGGPKSLGLELVRAKPMDEIEDDKVTIVGPDLKEM\r\nEEGKTYPWAMIFNIGGELVEPDLESVVERRVHDFINYCQGIMHLNQRYDVWMRVSKDTAA\r\nKMDSFEPFGQAVMMLFKTELPFIEKMQVTFYTEQAEVEKQLEEAKAIFKARDERTKDLHD\r\nEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFEITKGDLI\r\nDAEKGEYTGVNDIAKKLSAGEFDKIKLHSFFDAPHTSCGCFEVVGFYIPEVDGIGWVNRE\r\nYQGMAPNGIGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKDK\r\nIAETIPEDLKDKIATENDATDIESLKAFLQEKGHPVVATWAAAEEEEEEEEEEEEEVAVA\r\nAAPMMMPAAGFQMPAMPMMSGGSSGGIKLTFKNAKITIDKMIISEKKEKK", "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)", "uniprot_code": "Q8TJC4", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MAEFPFEISPMFEGERVRKEGMFVELGGPKSLGLELVRAKPMDEIEDDKVTIVGPDLKEM\nEEGKTYPWAMIFNIGGELVEPDLESVVERRVHDFINYCQGIMHLNQRYDVWMRVSKDTAA\nKMDSFEPFGQAVMMLFKTELPFIEKMQVTFYTEQAEVEKQLEEAKAIFKARDERTKDLHD\nEDVDVFYGCTLCQSFAPTNVCVVSPDRVSLCGAINWFDGRAAAKVDPEGPQFEITKGDLI\nDAEKGEYTGVNDIAKKLSAGEFDKIKLHSFFDAPHTSCGCFEVVGFYIPEVDGIGWVNRE\nYQGMAPNGIGFSTMAGQTGGGKQIVGFLGIGINYFYSPKFIQADGGWNRVVWLPSMLKDK\nIAETIPEDLKDKIATENDATDIESLKAFLQEKGHPVVATWAAAEEEEEEEEEEEEEVAVA\nAAPMMMPAAGFQMPAMPMMSGGSSGGIKLTFKNAKITIDKMIISEKKEKK", "mw": 52.426, "total_mw": 419.408, "number_molecules": 8, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit delta 2", "short_name": "CdhD", "sequence": "MAKKMKLSDITNMFAGMDVEALEGVTIEGDIEIDLGGLGGGFDPMLAAALGQESAILAQH\r\nFARLAGMFGYPVGIGAPAAPAVSPALAAPKLKDLIPAKFDVANIAEWATEIQEVPIGNTS\r\nADGGSRGKRVMLGGEKALPFYFDAPMPNRNQVTIDVFDMRIGLAKAVKENYDEVMDSPGE\r\nWAKKNVEKFNADMITIHLISTDPLIKDTPAKEAAKTVEEVLQAVDVPIAIGGSGNPQKDP\r\nEVLARAAEVSEGERCLLASASLNLDYAAIAEAALKYDHDVLSWTQLDMNAQKELNRKLMK\r\nQCNVPRDRIIMDPTTAALGYGLDYAYTNMERIRLAALMGDDELTFPMSSGTTNAWGARES\r\nWMVSSPLKEDSDWGPREYRGPIWEIVTGLSLAIAGNDLFMMMHPTSVAVLKQITQTLFGM\r\nIDTEQVDVANWIGAEV", "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)", "uniprot_code": "Q8TJC2", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MAKKMKLSDITNMFAGMDVEALEGVTIEGDIEIDLGGLGGGFDPMLAAALGQESAILAQH\nFARLAGMFGYPVGIGAPAAPAVSPALAAPKLKDLIPAKFDVANIAEWATEIQEVPIGNTS\nADGGSRGKRVMLGGEKALPFYFDAPMPNRNQVTIDVFDMRIGLAKAVKENYDEVMDSPGE\nWAKKNVEKFNADMITIHLISTDPLIKDTPAKEAAKTVEEVLQAVDVPIAIGGSGNPQKDP\nEVLARAAEVSEGERCLLASASLNLDYAAIAEAALKYDHDVLSWTQLDMNAQKELNRKLMK\nQCNVPRDRIIMDPTTAALGYGLDYAYTNMERIRLAALMGDDELTFPMSSGTTNAWGARES\nWMVSSPLKEDSDWGPREYRGPIWEIVTGLSLAIAGNDLFMMMHPTSVAVLKQITQTLFGM\nIDTEQVDVANWIGAEV", "mw": 47.082, "total_mw": 376.656, "number_molecules": 8, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "Acetyl-CoA decarbonylase/synthase complex subunit gamma", "short_name": "CdhE", "sequence": ">Q8TH44\r\nMKINSPLEAYKYLPQTNCGECGEATCMAFASKLIDRSGKTSDCPPLIKEKKFAKKLAELD\r\nRLLAPEIRQVTIGVGEKAVNIGGDDVLYRHKLTFFNKTKMFFDVADNMDEAALVERVNSI\r\nANFRKFYVGRNLLLDGVAIRAVSNDPAKFAAAVKKVAEAGLPMIFCSFNPAVLKAGLEAA\r\nKDLKPLLYAANKDNWKEVGELAIEYKVPVVVSAFNDLDALKTLAKTYAEAGIKDIVLDPG\r\nTYPTGKGLKDTFTNFLKIRRAGIMGDTEIAYPIMALPFTAWMAGIADPVSASYWETVMAS\r\nVFTIRYGDIMILHSLEPYATLPEVHLAETIYTDPRTPVSVDGGMYKVGSPTADSPVLFTT\r\nNFALTYYTVESDISSNGIDCWLLAVDTDGIGVEAAVAGGQLTADKVKDAFDKAGFDLKTA\r\nVNHNTVVTPGLAARLQGDLEDKLGANVKVGPMDSGRIPGWMEKNWPPK", "organism": "Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)", "uniprot_code": "Q8TH44", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "other", "molecular_type": "protein", "uniprot_sequence": "MKINSPLEAYKYLPQTNCGECGEATCMAFASKLIDRSGKTSDCPPLIKEKKFAKKLAELD\nRLLAPEIRQVTIGVGEKAVNIGGDDVLYRHKLTFFNKTKMFFDVADNMDEAALVERVNSI\nANFRKFYVGRNLLLDGVAIRAVSNDPAKFAAAVKKVAEAGLPMIFCSFNPAVLKAGLEAA\nKDLKPLLYAANKDNWKEVGELAIEYKVPVVVSAFNDLDALKTLAKTYAEAGIKDIVLDPG\nTYPTGKGLKDTFTNFLKIRRAGIMGDTEIAYPIMALPFTAWMAGIADPVSASYWETVMAS\nVFTIRYGDIMILHSLEPYATLPEVHLAETIYTDPRTPVSVDGGMYKVGSPTADSPVLFTT\nNFALTYYTVESDISSNGIDCWLLAVDTDGIGVEAAVAGGQLTADKVKDAFDKAGFDLKTA\nVNHNTVVTPGLAARLQGDLEDKLGANVKVGPMDSGRIPGWMEKNWPPK", "mw": 50.794, "total_mw": 406.352, "number_molecules": 8, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "50 mM MOPS, 400 mM NaCl, 2 % (w/v) sucrose, 2 mM reduced glutathione (GSH), 2 mM DTT, 2 mM NaNO3, 2 mM ascorbic acid", "concentration_unit": null, "comment": "Buffer optimized to reduce radiation and oxidative damage", "additive": null, "concentration": null, "pka": null, "ph": 7.2, "deuteration": null }, "purity_method": null, "name": "Acetyl-CoA decarbonylase/synthase (ACDS), natively purified from Methanosarcina acetivorans", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Marburg, Germany", "full_name": "Philipps-Universität Marburg", "webpage": "" } ], "contributor_name": "Erik", "contributor_surname": "Zimmer", "orcid": "https://orcid.org/0009-0005-9366-5283" } ], "concentration_method": null, "concentration_unit": null, "date": "2025-09-07", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 2.0, "number_of_frames": 600, "wavelength": 0.1, "sample_detector_distance": 2.824, "concentration_min": null, "concentration_max": 5.0, "sample_volume": 100.0, "flow_rate": 0.25, "s_min": 0.056, "s_max": 5.58, "total_exposure_time": null, "seccolumn": 10 }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.1.1", "i0_calibration_standard": null, "description": null, "experiment_description": "Each of the Acetyl-CoA decarbonylase/synthase five subunits (alpha, beta, gamma, delta, epsilon) occurs eight times in the complex, so the total stoichiometry is CdhA8B8C8D8E8.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 2181.63, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 556.5, "pddf_i0_error": null, "guinier_i0": 563.33, "guinier_i0_error": null, "pddf_rg": 10.59, "pddf_rg_error": null, "guinier_rg": 10.85, "guinier_rg_error": 0.041, "pddf_dmax": 31.44, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 12, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASD2C2_datloglog_img.png", "symmetry": null, "last_modified": "2026-07-29T16:40:20.923692+02:00", "bragg_peak": [] }