data_SASDGC3_MAIN # _sas_result.id 1579 _sas_result.SASBDB_code SASDGC3 _sas_result.experimental_MW 69.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier . _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR 2.000 _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier . _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min . _sas_result.Guinier_point_max . _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "his curve is part of a pH series. Increasing the pH removes aggregation presumably due to protein surface charge changes. The SAXS data for this entry is measured from aggregated material and therefore the Rg and I(0) parameters do not apply." # _sas_scan.title "DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)" _sas_scan.detc_id 20 _sas_scan.id 1579 _sas_scan.measurement_date 2018-11-02 _sas_scan.storage_temperature 10.0 _sas_scan.cell_temperature 10.0 _sas_scan.exposure_time 3.000 _sas_scan.number_of_frames 300 _sas_scan.unit 1/A _sas_scan.qmin 0.0081 _sas_scan.qmax 0.3921 _sas_scan.sample_id 1540 _sas_scan.result_id 1579 _sas_scan.beam_id 21 # _sas_beam.id 21 _sas_beam.instrument_name "Advanced Light Source (ALS) 12.3.1 (SIBYLS)" _sas_beam.instrument_city "Berkeley, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.103000 _sas_beam.dist_spec_to_detc 1.50000 # _sas_detc.id 20 _sas_detc.name "Pilatus3 X 2M" _sas_detc.type . _sas_detc.pixsize_x 172.000 # _sas_sample.id 1540 _sas_sample.name "DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration . _sas_sample.concentration_method . loop_ _sas_sample.entity_id 1622 1623 1632 # _sas_buffer.id 851 _sas_buffer.name "10 mM Bis-Tris, 50 mM NaCl" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 5.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1540 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 1622 DNA "80bp_DNA Forward" 24.849 1 1623 DNA "80bp_DNA Reverse" 24.612 1 1632 protein "DNA-binding protein HU-alpha, E34K" 9.534 2 # loop_ _entity_name_com.entity_id _entity_name_com.name 1622 . 1623 . 1632 . # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 1622 AATGAGGTAACAACGAAAGCAGATGATAGCTGCTTATCAATTTGTTGCAAACAAGTAGCCGCGCCCAATGAGGTAACAAT 1623 TTACTCCATTGTTGCTTTCGTCTACTATCGACGAATAGTTAAACAACGTTTGTTCATCGGCGCGGGTTACTCCATTGTTA 1632 ;MNKTQLIDVIAEKAELSKTQAKAALESTLAAITKSLKEGDAVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFVSGKALKDAVK ; # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 1622 "Escherichia coli" 1623 "Escherichia coli K-12" 1632 "Escherichia coli K-12" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code 1 1622 UniProt . 2 1623 UniProt . 3 1632 UniProt P0ACF0 _struct_ref.details http://www.uniprot.org/uniprot/P0ACF0 # _pdbx_contact_author.id 240 _pdbx_contact_author.name_first Soumya _pdbx_contact_author.name_last "G Remesh" _pdbx_contact_author.role . _pdbx_contact_author.address_1 . _pdbx_contact_author.address_2 "Lawrence Berkeley National laboratory" _pdbx_contact_author.address_3 "Berkeley, CA, USA" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 8.083000E-03 2.992266E+03 2.442586E+01 1579 2 8.640000E-03 2.505405E+03 5.192784E+01 1579 3 9.198000E-03 2.039646E+03 3.673909E+01 1579 4 9.755000E-03 1.690268E+03 2.940296E+01 1579 5 1.031300E-02 1.402095E+03 2.619793E+01 1579 6 1.087000E-02 1.430556E+03 5.342552E+01 1579 7 1.142800E-02 1.274461E+03 4.132395E+01 1579 8 1.198500E-02 1.100021E+03 2.856119E+01 1579 9 1.254300E-02 9.148071E+02 2.016181E+01 1579 10 1.310000E-02 7.738480E+02 1.833037E+01 1579 11 1.365800E-02 6.608053E+02 1.498014E+01 1579 12 1.421500E-02 5.718061E+02 1.476468E+01 1579 13 1.477200E-02 4.782682E+02 1.262748E+01 1579 14 1.533000E-02 4.309824E+02 1.313755E+01 1579 15 1.588700E-02 3.745906E+02 1.151239E+01 1579 16 1.644500E-02 3.308720E+02 9.523658E+00 1579 17 1.700200E-02 2.940045E+02 8.959831E+00 1579 18 1.756000E-02 2.557111E+02 8.626460E+00 1579 19 1.811700E-02 2.280188E+02 7.652718E+00 1579 20 1.867500E-02 2.026824E+02 8.171567E+00 1579 21 1.923200E-02 1.844354E+02 6.801269E+00 1579 22 1.978900E-02 1.682182E+02 5.587876E+00 1579 23 2.034700E-02 1.521499E+02 4.300692E+00 1579 24 2.090400E-02 1.390144E+02 3.934210E+00 1579 25 2.146200E-02 1.277428E+02 3.501366E+00 1579 26 2.201900E-02 1.195636E+02 2.718164E+00 1579 27 2.257700E-02 1.113886E+02 2.429232E+00 1579 28 2.313400E-02 1.005044E+02 2.010617E+00 1579 29 2.369200E-02 9.331808E+01 1.886962E+00 1579 30 2.424900E-02 9.031411E+01 1.526014E+00 1579 31 2.480700E-02 8.308570E+01 1.432341E+00 1579 32 2.536400E-02 7.896569E+01 1.212550E+00 1579 33 2.592100E-02 7.371374E+01 1.118269E+00 1579 34 2.647900E-02 7.148350E+01 9.534350E-01 1579 35 2.703600E-02 6.485907E+01 8.722590E-01 1579 36 2.759400E-02 6.333862E+01 8.289640E-01 1579 37 2.815100E-02 6.219329E+01 7.633810E-01 1579 38 2.870900E-02 5.802242E+01 7.020900E-01 1579 39 2.926600E-02 5.654429E+01 6.841690E-01 1579 40 2.982400E-02 5.422963E+01 6.542840E-01 1579 41 3.038100E-02 5.045001E+01 6.059050E-01 1579 42 3.093800E-02 4.950817E+01 5.873680E-01 1579 43 3.149600E-02 4.859709E+01 5.939580E-01 1579 44 3.205300E-02 4.624637E+01 5.876170E-01 1579 45 3.261100E-02 4.433840E+01 5.365520E-01 1579 46 3.316800E-02 4.465765E+01 5.688810E-01 1579 47 3.372600E-02 4.132199E+01 5.467940E-01 1579 48 3.428300E-02 4.032233E+01 5.349970E-01 1579 49 3.484100E-02 3.932439E+01 5.280690E-01 1579 50 3.539800E-02 3.784632E+01 5.137980E-01 1579 51 3.595500E-02 3.652173E+01 5.071400E-01 1579 52 3.651300E-02 3.601550E+01 4.938330E-01 1579 53 3.707000E-02 3.609854E+01 4.798210E-01 1579 54 3.762800E-02 3.561194E+01 5.184870E-01 1579 55 3.818500E-02 3.299273E+01 4.874010E-01 1579 56 3.874300E-02 3.275636E+01 5.025600E-01 1579 57 3.930000E-02 3.258291E+01 4.584840E-01 1579 58 3.985800E-02 3.175964E+01 4.507300E-01 1579 59 4.041500E-02 2.980405E+01 4.439090E-01 1579 60 4.097300E-02 3.084882E+01 4.367950E-01 1579 61 4.153000E-02 2.954905E+01 4.539610E-01 1579 62 4.208700E-02 2.928285E+01 4.340960E-01 1579 63 4.264500E-02 2.825194E+01 4.331450E-01 1579 64 4.320200E-02 2.759997E+01 4.174110E-01 1579 65 4.376000E-02 2.745542E+01 4.124720E-01 1579 66 4.431700E-02 2.615238E+01 4.430960E-01 1579 67 4.487500E-02 2.726681E+01 4.040740E-01 1579 68 4.543200E-02 2.653868E+01 4.198990E-01 1579 69 4.599000E-02 2.512048E+01 3.860200E-01 1579 70 4.654700E-02 2.576920E+01 4.061480E-01 1579 71 4.710400E-02 2.409776E+01 3.718660E-01 1579 72 4.766200E-02 2.516213E+01 3.784600E-01 1579 73 4.821900E-02 2.384537E+01 3.762910E-01 1579 74 4.877700E-02 2.376408E+01 3.833550E-01 1579 75 4.933400E-02 2.348911E+01 3.782390E-01 1579 76 4.989200E-02 2.331396E+01 3.633770E-01 1579 77 5.044900E-02 2.208338E+01 3.617570E-01 1579 78 5.100700E-02 2.214468E+01 3.689230E-01 1579 79 5.156400E-02 2.268546E+01 3.615400E-01 1579 80 5.212100E-02 2.147631E+01 3.596070E-01 1579 81 5.267900E-02 2.137493E+01 3.502780E-01 1579 82 5.323600E-02 2.168444E+01 3.449550E-01 1579 83 5.379400E-02 2.104208E+01 3.596860E-01 1579 84 5.435100E-02 2.080417E+01 3.566780E-01 1579 85 5.490900E-02 1.951465E+01 3.393020E-01 1579 86 5.546600E-02 2.001915E+01 3.361680E-01 1579 87 5.602400E-02 2.071812E+01 3.410320E-01 1579 88 5.658100E-02 1.882032E+01 3.370350E-01 1579 89 5.713800E-02 1.925270E+01 3.396550E-01 1579 90 5.769600E-02 1.967107E+01 3.369980E-01 1579 91 5.825300E-02 1.863249E+01 3.319790E-01 1579 92 5.881100E-02 1.803882E+01 3.371760E-01 1579 93 5.936800E-02 1.798883E+01 3.203190E-01 1579 94 5.992600E-02 1.692009E+01 3.129670E-01 1579 95 6.048300E-02 1.720006E+01 3.119750E-01 1579 96 6.104100E-02 1.727048E+01 3.179780E-01 1579 97 6.159800E-02 1.768485E+01 3.232190E-01 1579 98 6.215500E-02 1.739532E+01 3.030130E-01 1579 99 6.271300E-02 1.710127E+01 3.093390E-01 1579 100 6.327000E-02 1.619524E+01 3.078930E-01 1579 101 6.382800E-02 1.722751E+01 3.089270E-01 1579 102 6.438500E-02 1.667274E+01 2.933090E-01 1579 103 6.494300E-02 1.597494E+01 3.093410E-01 1579 104 6.550000E-02 1.605598E+01 3.028560E-01 1579 105 6.605700E-02 1.489438E+01 2.925000E-01 1579 106 6.661500E-02 1.529709E+01 2.842830E-01 1579 107 6.717200E-02 1.466613E+01 2.874510E-01 1579 108 6.773000E-02 1.529193E+01 2.882650E-01 1579 109 6.828700E-02 1.443388E+01 2.803130E-01 1579 110 6.884500E-02 1.435769E+01 2.727030E-01 1579 111 6.940200E-02 1.381079E+01 2.842640E-01 1579 112 6.996000E-02 1.479382E+01 2.883240E-01 1579 113 7.051700E-02 1.425638E+01 2.771130E-01 1579 114 7.107400E-02 1.442498E+01 2.755330E-01 1579 115 7.163200E-02 1.404417E+01 2.888560E-01 1579 116 7.218900E-02 1.470273E+01 2.777190E-01 1579 117 7.274700E-02 1.318972E+01 2.717200E-01 1579 118 7.330400E-02 1.414675E+01 2.760150E-01 1579 119 7.386200E-02 1.306711E+01 2.835410E-01 1579 120 7.441900E-02 1.262431E+01 2.831430E-01 1579 121 7.497700E-02 1.226547E+01 2.816400E-01 1579 122 7.553400E-02 1.318808E+01 2.801220E-01 1579 123 7.609100E-02 1.264616E+01 2.715590E-01 1579 124 7.664900E-02 1.257829E+01 2.809320E-01 1579 125 7.720600E-02 1.202493E+01 2.700050E-01 1579 126 7.776400E-02 1.178573E+01 2.737420E-01 1579 127 7.832100E-02 1.297770E+01 2.653830E-01 1579 128 7.887900E-02 1.201613E+01 2.702690E-01 1579 129 7.943600E-02 1.163241E+01 2.716120E-01 1579 130 7.999300E-02 1.099674E+01 2.729420E-01 1579 131 8.055100E-02 1.109922E+01 2.549270E-01 1579 132 8.110800E-02 1.158244E+01 2.597460E-01 1579 133 8.166600E-02 1.088959E+01 2.644200E-01 1579 134 8.222300E-02 1.124314E+01 2.574370E-01 1579 135 8.278100E-02 1.065557E+01 2.619560E-01 1579 136 8.333800E-02 1.047482E+01 2.623760E-01 1579 137 8.389500E-02 1.037442E+01 2.466550E-01 1579 138 8.445300E-02 1.087963E+01 2.621980E-01 1579 139 8.501000E-02 9.535060E+00 2.576770E-01 1579 140 8.556800E-02 1.079573E+01 2.476970E-01 1579 141 8.612500E-02 1.032615E+01 2.583780E-01 1579 142 8.668300E-02 1.016324E+01 2.426450E-01 1579 143 8.724000E-02 9.977412E+00 2.400800E-01 1579 144 8.779800E-02 1.056249E+01 2.525660E-01 1579 145 8.835500E-02 9.903571E+00 2.422730E-01 1579 146 8.891200E-02 1.016011E+01 2.513810E-01 1579 147 8.947000E-02 9.123618E+00 2.438870E-01 1579 148 9.002700E-02 1.012346E+01 2.503010E-01 1579 149 9.058500E-02 9.885408E+00 2.403730E-01 1579 150 9.114200E-02 8.878602E+00 2.434930E-01 1579 151 9.170000E-02 9.874336E+00 2.361830E-01 1579 152 9.225700E-02 8.903870E+00 2.435630E-01 1579 153 9.281400E-02 8.739095E+00 2.359870E-01 1579 154 9.337200E-02 9.191804E+00 2.456430E-01 1579 155 9.392900E-02 8.920929E+00 2.614970E-01 1579 156 9.448700E-02 9.019404E+00 2.566510E-01 1579 157 9.504400E-02 8.748826E+00 2.579760E-01 1579 158 9.560200E-02 8.350902E+00 2.634560E-01 1579 159 9.615900E-02 8.099096E+00 2.650060E-01 1579 160 9.671600E-02 8.574037E+00 2.731260E-01 1579 161 9.727400E-02 8.759552E+00 2.670840E-01 1579 162 9.783100E-02 8.558796E+00 2.576230E-01 1579 163 9.838900E-02 9.074870E+00 2.683670E-01 1579 164 9.894600E-02 8.290014E+00 2.691190E-01 1579 165 9.950400E-02 7.789371E+00 2.636840E-01 1579 166 1.000610E-01 7.412531E+00 2.537120E-01 1579 167 1.006180E-01 8.248977E+00 2.583100E-01 1579 168 1.011760E-01 7.528708E+00 2.502700E-01 1579 169 1.017330E-01 8.307705E+00 2.455920E-01 1579 170 1.022910E-01 7.450320E+00 2.501200E-01 1579 171 1.028480E-01 7.717268E+00 2.425110E-01 1579 172 1.034060E-01 7.980731E+00 2.445140E-01 1579 173 1.039630E-01 7.964038E+00 2.381590E-01 1579 174 1.045200E-01 7.699402E+00 2.353520E-01 1579 175 1.050780E-01 7.835610E+00 2.343490E-01 1579 176 1.056350E-01 7.811985E+00 2.326610E-01 1579 177 1.061930E-01 6.923774E+00 2.333570E-01 1579 178 1.067500E-01 7.311169E+00 2.295480E-01 1579 179 1.073080E-01 6.656675E+00 2.305270E-01 1579 180 1.078650E-01 7.628256E+00 2.230270E-01 1579 181 1.084220E-01 6.944974E+00 2.326660E-01 1579 182 1.089800E-01 6.717840E+00 2.284880E-01 1579 183 1.095370E-01 7.211641E+00 2.124410E-01 1579 184 1.100950E-01 6.608511E+00 2.245740E-01 1579 185 1.106520E-01 7.124221E+00 2.292800E-01 1579 186 1.112100E-01 6.583830E+00 2.251540E-01 1579 187 1.117670E-01 6.572186E+00 2.178310E-01 1579 188 1.123240E-01 6.460297E+00 2.217100E-01 1579 189 1.128820E-01 6.596250E+00 2.168980E-01 1579 190 1.134390E-01 6.088539E+00 2.181160E-01 1579 191 1.139970E-01 6.633372E+00 2.130960E-01 1579 192 1.145540E-01 6.493046E+00 2.131160E-01 1579 193 1.151110E-01 6.520345E+00 2.172900E-01 1579 194 1.156690E-01 6.357052E+00 2.121650E-01 1579 195 1.162260E-01 5.795490E+00 2.062160E-01 1579 196 1.167840E-01 5.859593E+00 2.086280E-01 1579 197 1.173410E-01 6.274204E+00 2.107490E-01 1579 198 1.178990E-01 6.807343E+00 2.069940E-01 1579 199 1.184560E-01 5.896331E+00 2.061980E-01 1579 200 1.190130E-01 6.008275E+00 2.006770E-01 1579 201 1.195710E-01 6.085874E+00 2.051780E-01 1579 202 1.201280E-01 6.104426E+00 1.974450E-01 1579 203 1.206860E-01 6.340285E+00 1.963980E-01 1579 204 1.212430E-01 5.767668E+00 1.997700E-01 1579 205 1.218000E-01 6.037245E+00 2.040270E-01 1579 206 1.223580E-01 6.357966E+00 1.985460E-01 1579 207 1.229150E-01 5.324951E+00 1.963800E-01 1579 208 1.234730E-01 5.883052E+00 2.062130E-01 1579 209 1.240300E-01 5.261221E+00 2.014100E-01 1579 210 1.245880E-01 5.487199E+00 1.942210E-01 1579 211 1.251450E-01 5.346078E+00 1.919020E-01 1579 212 1.257020E-01 5.343070E+00 2.024880E-01 1579 213 1.262600E-01 5.420759E+00 1.924970E-01 1579 214 1.268170E-01 5.315413E+00 1.920040E-01 1579 215 1.273750E-01 5.387018E+00 1.955950E-01 1579 216 1.279320E-01 5.364784E+00 1.899210E-01 1579 217 1.284890E-01 5.655302E+00 1.934830E-01 1579 218 1.290470E-01 5.070940E+00 1.892150E-01 1579 219 1.296040E-01 5.666112E+00 1.881110E-01 1579 220 1.301620E-01 4.835571E+00 1.911600E-01 1579 221 1.307190E-01 5.134939E+00 1.897210E-01 1579 222 1.312770E-01 5.348117E+00 1.922720E-01 1579 223 1.318340E-01 4.521229E+00 1.854200E-01 1579 224 1.323910E-01 5.204415E+00 2.083250E-01 1579 225 1.329490E-01 4.988476E+00 2.174320E-01 1579 226 1.335060E-01 4.421198E+00 2.222930E-01 1579 227 1.340640E-01 4.081307E+00 2.283770E-01 1579 228 1.346210E-01 4.213616E+00 2.808310E-01 1579 229 1.351780E-01 5.095621E+00 2.436210E-01 1579 230 1.357360E-01 4.379729E+00 2.243780E-01 1579 231 1.362930E-01 4.987506E+00 2.413910E-01 1579 232 1.368510E-01 4.118011E+00 2.379550E-01 1579 233 1.374080E-01 4.410925E+00 2.895060E-01 1579 234 1.379650E-01 4.711699E+00 2.293470E-01 1579 235 1.385230E-01 3.802222E+00 2.092710E-01 1579 236 1.390800E-01 4.335082E+00 1.998970E-01 1579 237 1.396380E-01 4.106385E+00 2.130430E-01 1579 238 1.401950E-01 4.049811E+00 1.981290E-01 1579 239 1.407520E-01 4.123510E+00 1.941410E-01 1579 240 1.413100E-01 3.795972E+00 1.962480E-01 1579 241 1.418670E-01 3.891262E+00 2.089740E-01 1579 242 1.424250E-01 3.867581E+00 1.936900E-01 1579 243 1.429820E-01 3.628020E+00 2.002550E-01 1579 244 1.435400E-01 3.961169E+00 2.062380E-01 1579 245 1.440970E-01 4.225550E+00 2.118390E-01 1579 246 1.446540E-01 3.595622E+00 2.615590E-01 1579 247 1.452120E-01 3.421937E+00 2.248720E-01 1579 248 1.457690E-01 3.271503E+00 2.197130E-01 1579 249 1.463270E-01 4.119432E+00 2.091550E-01 1579 250 1.468840E-01 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