{ "code": "SASDFC5", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/nm", "project": { "title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.", "publication": { "title": "Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.", "author_list": "Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K", "journal": "J Struct Biol", "doi": "10.1016/j.jsb.2019.09.007", "pmid": "31550533", "published_date": "2019 Sep 21" }, "status": "released", "submitted_date": "2019-04-08", "released_date": "2019-11-14" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFC5.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFC5.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFC5_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFC5.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Enhanced disease susceptibility", "short_name": "EDS1", "sequence": ">sp|Q9SU72|EDS1C_ARATH Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1\r\nMAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\r\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\r\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\r\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\r\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\r\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\r\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\r\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\r\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\r\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\r\nTLPKNHKSHSPLRDYMMDEITDT", "organism": "Arabidopsis thaliana", "uniprot_code": "Q9SU72", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\nTLPKNHKSHSPLRDYMMDEITDT", "mw": 71.689, "total_mw": 71.689, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Enhanced disease susceptibility 1 (EDS1)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Germany", "full_name": "University of Cologne", "webpage": "http://uni-koeln.de/" } ], "contributor_name": "Martin", "contributor_surname": "Voss", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2015-05-19", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 2.0, "number_of_frames": 1495, "wavelength": 0.099, "sample_detector_distance": 2.867, "concentration_min": null, "concentration_max": 5.0, "sample_volume": null, "flow_rate": null, "s_min": 0.036, "s_max": 4.939, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFC5_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFC5_fit1_model1.pdb", "model_mw": 57.855, "bead_radius": 2.6, "log": null, "symmetry": null, "comment": null, "user": 414 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/EDS1komp_fixed_fit_img.png", "software": "DAMMIN", "chi_square_value": 0.824, "p_value": 0.059642, "fit_residual_plot": "EDS1komp_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFC5_fit1.fir", "fit_log": null, "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": "The data were re-binned to calculate the p(r) profile and corresponding model fit.", "experiment_description": "Synchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4 mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 72.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 58.0, "porod_mw_error": null, "pddf_i0": 152.6, "pddf_i0_error": null, "guinier_i0": 151.87, "guinier_i0_error": 0.47, "pddf_rg": 3.08, "pddf_rg_error": null, "guinier_rg": 3.09, "guinier_rg_error": 0.02, "pddf_dmax": 10.53, "pddf_dmax_error": null, "porod_volume": 92.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 23, "guinier_point_last": 80, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDFC5_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }