{ "code": "SASDVC5", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/A", "project": { "title": "The sequence–structure–function relationship of intrinsic ERα disorder", "publication": { "title": "The sequence–structure–function relationship of intrinsic ERα disorder", "author_list": "Du Z, Wang H, Luo S, Yun Z, Wu C, Yang W, Buck M, Zheng W, Hansen A, Kao H, Yang S", "journal": "Nature", "doi": "10.1038/s41586-024-08400-1", "pmid": null, "published_date": "2025 Jan 08" }, "status": "released", "submitted_date": "2024-07-23", "released_date": "2024-12-13" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVC5.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVC5_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVC5.sascif", "experiment": { "instrument": { "detector": { "type": "Duel detector", "name": "Pilatus3 S 1M, Pilatus3 900 K", "resolution": null }, "name": "National Synchrotron Light Source II (NSLS-II)", "city": "Upton, NY", "country": "USA", "beamline_name": "16-ID (LiX)", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Estrogen receptor", "short_name": "ER-NTD", "sequence": "SNAMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRY", "organism": "Homo sapiens", "uniprot_code": "P03372", "uniprot_range_first": 1, "uniprot_range_last": 184, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAY\nEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPF\nLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK\nETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC\nRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR\nSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW\nAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG\nMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD\nKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL\nLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV", "mw": 20.175, "total_mw": 20.175, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "SEC-SAXS data of phosphorylated estrogen receptor alpha (pSer118), N-terminal domain (NTD) at pH 7.4", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Cleveland, USA", "full_name": "Case Western Reserve University", "webpage": "https://case.edu/" } ], "contributor_name": "Sichun", "contributor_surname": "Yang", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2024-07-17", "storage_temperature": 8.0, "cell_temperature": 4.0, "exposure_time": 2.0, "number_of_frames": 360, "wavelength": 0.08189, "sample_detector_distance": 4.003, "concentration_min": null, "concentration_max": 2.0, "sample_volume": 100.0, "flow_rate": 0.35, "s_min": 0.1, "s_max": 2.5, "total_exposure_time": null, "seccolumn": 54 }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of the phosphorylated N-terminal domain of estrogen receptor alpha (pSer118) in 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M, Pilatus3 900 K detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 2 mg/ml was injected at a 0.35 ml/min flow rate onto a Cytiva Superdex 75 Increase 5/150 column at 4°C. 360 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 20.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 43.0, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 3.83022, "guinier_i0_error": null, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 3.635, "guinier_rg_error": 0.607, "pddf_dmax": 16.0, "pddf_dmax_error": null, "porod_volume": 70.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 6, "guinier_point_last": 30, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDVC5_datloglog_img.png", "symmetry": null, "last_modified": "2025-01-08T17:59:15.079200+01:00", "bragg_peak": [] }