{ "code": "SASDCC7", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.", "publication": { "title": "Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima.", "author_list": "Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G", "journal": "Structure", "doi": "10.1016/j.str.2017.10.001", "pmid": "29107484", "published_date": "2017 Dec 5" }, "status": "released", "submitted_date": "2017-09-01", "released_date": "2017-10-31" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCC7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCC7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCC7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCC7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCC7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "T.maritima PDE", "short_name": "TmPDE D80N D154N", "sequence": "GSGSGMDEIVKVLSQHDRILVVGHIMPDGDCVSSVLSLTLGLEKLGKEVKAAVDYKIPYVFEKFPYIDKIEENPNFDPELLVVVNASSPDRIGKFQDLLDKVPSVVIDHHSTNTNFGNWNWVDPSFAATAQMIFRINKALGVEYDSNLATLNYLGIATNTGFFRHSNADVRVFEDAYKLVKMGADAHFVAKEILENKRFEQFKLFAEVLERLQLLENGKIAYSYIDYDTYLRHNCTDEDSAGFVGELRSIRGVEVAVLFMEFPRGKIHVSMRSKDWFNVNEVAFELGGGGHPRAAGVTFEGKKIEEVIPRVINHLLKKFKEGVESESEKIPEGDVLGG", "organism": "Thermotoga maritima", "uniprot_code": "Q9X1T1", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MDEIVKVLSQHDRILVVGHIMPDGDCVSSVLSLTLGLEKLGKEVKAAVDYKIPYVFEKFP\nYIDKIEENPNFDPELLVVVDASSPDRIGKFQDLLDKVPSVVIDHHSTNTNFGNWNWVDPS\nFAATAQMIFRINKALGVEYDSNLATLNYLGIATDTGFFRHSNADVRVFEDAYKLVKMGAD\nAHFVAKEILENKRFEQFKLFAEVLERLQLLENGKIAYSYIDYDTYLRHNCTDEDSAGFVG\nELRSIRGVEVAVLFMEFPRGKIHVSMRSKDWFNVNEVAFELGGGGHPRAAGVTFEGKKIE\nEVIPRVINHLLKKFKEGVESESEKIPEGDVLGG", "mw": 37.821, "total_mw": 75.642, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Munich, Germany", "full_name": "Ludwig-Maximilians-Universität München", "webpage": "https://www.en.uni-muenchen.de" } ], "contributor_name": "Gregor", "contributor_surname": "Witte", "orcid": "https://orcid.org/0000-0003-0313-0962" } ], "concentration_method": null, "concentration_unit": null, "date": "2016-06-17", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 0.995, "number_of_frames": null, "wavelength": 0.124, "sample_detector_distance": 3.0, "concentration_min": null, "concentration_max": null, "sample_volume": null, "flow_rate": null, "s_min": 0.0, "s_max": 2.927, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant) in 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH 8, were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Samples were collected using SEC-SAXS at 20°C. The scattering was obtained by averaging the main peak's scattering data of a SECSAXS run with TmPDE D80ND154N on a 10/300 S200 superdex column. The data was corrected for buffer scattering by subtraction of an averaged buffer region using the CHROMIXS software (ATSAS 2.8.1).", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 72.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 69.2, "porod_mw_error": null, "pddf_i0": 9314.0, "pddf_i0_error": null, "guinier_i0": 9293.71, "guinier_i0_error": 6.68, "pddf_rg": 2.78, "pddf_rg_error": null, "guinier_rg": 2.79, "guinier_rg_error": 0.03, "pddf_dmax": 7.9, "pddf_dmax_error": null, "porod_volume": 115.38, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 54, "guinier_point_last": 175, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCC7_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }