data_SASDDC7_MAIN # _sas_result.id 939 _sas_result.SASBDB_code SASDDC7 _sas_result.experimental_MW 55.030 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 36.5900000000 _sas_result.I0_from_Guinier_error 0.0400000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 3.910 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 20 _sas_result.Guinier_point_max 42 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "Synthetic FRET biosensor Twitch 2B N532F Ca-free" _sas_scan.detc_id 16 _sas_scan.id 939 _sas_scan.measurement_date 2016-06-30 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 12 _sas_scan.unit 1/nm _sas_scan.qmin 0.0346 _sas_scan.qmax 4.9518 _sas_scan.sample_id 899 _sas_scan.result_id 939 _sas_scan.beam_id 10 # _sas_beam.id 10 _sas_beam.instrument_name "ESRF BM29" _sas_beam.instrument_city Grenoble _sas_beam.instrument_country France _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.099500 _sas_beam.dist_spec_to_detc 2.86700 # _sas_detc.id 16 _sas_detc.name "Pilatus 1M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 899 _sas_sample.name "Synthetic FRET biosensor Twitch 2B N532F Ca-free" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 5.00 _sas_sample.concentration_method . _sas_sample.entity_id 1087 # _sas_buffer.id 560 _sas_buffer.name "20 mM MOPS 100 mM NaCl 10 mM EDTA" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.000 _sas_buffer.comment "Buffer B" _sas_buffer.composition . _sas_buffer.sample_id 899 # _entity.id 1087 _entity.type protein _entity.pdbx_description "Synthetic FRET biosensor Twitch2B N532F" _entity.formula_weight 62.432 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1087 _entity_name_com.name TW6 # _entity_poly.entity_id 1087 _entity_poly.pdbx_seq_one_letter_code ;MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT TGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGN VYITADKQKNGIKANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSTQSKLSKDPNEKRDHMVLLEFVTAARMQVADASEEELSECFRIFDFD GNGFIDREEFGDIIRLTGEQLTDEDVDEIFGDSDTDKNGRIDFDEFLKMV ENVQPIYPELMGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPN EKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVE LDGDVNGHKFSVRGEGEGDATNGKLTLKLICTTGKLPVPWPTLVTTLGYG LMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHFVYITADKQKNGIKANFKI RHNIED ; # _entity_src_gen.entity_id 1087 _entity_src_gen.gene_src_common_name "synthetic construct" # _struct_ref.id 1 _struct_ref.entity_id 1087 _struct_ref.db_name UniProt _struct_ref.db_code . # _pdbx_contact_author.id 266 _pdbx_contact_author.name_first Pablo _pdbx_contact_author.name_last "Trigo Mourino" _pdbx_contact_author.role . _pdbx_contact_author.address_1 . _pdbx_contact_author.address_2 "Max Planck Institute for Biophysical Chemistry" _pdbx_contact_author.address_3 . # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 3.460564E-02 2.478872E+01 1.791466E+00 939 2 3.932468E-02 2.998056E+01 8.399607E-01 939 3 4.404371E-02 3.599894E+01 6.335722E-01 939 4 4.876275E-02 3.775190E+01 5.009156E-01 939 5 5.348178E-02 3.698455E+01 4.047975E-01 939 6 5.820081E-02 3.699903E+01 3.360766E-01 939 7 6.291985E-02 3.711283E+01 2.826577E-01 939 8 6.763888E-02 3.638734E+01 2.436554E-01 939 9 7.235792E-02 3.552823E+01 2.137860E-01 939 10 7.707695E-02 3.576607E+01 1.910590E-01 939 11 8.179598E-02 3.579164E+01 1.724869E-01 939 12 8.651502E-02 3.557033E+01 1.577044E-01 939 13 9.123405E-02 3.532141E+01 1.459626E-01 939 14 9.595308E-02 3.508832E+01 1.360339E-01 939 15 1.006721E-01 3.491109E+01 1.271212E-01 939 16 1.053912E-01 3.463823E+01 1.195543E-01 939 17 1.101102E-01 3.459489E+01 1.136361E-01 939 18 1.148292E-01 3.417146E+01 1.083266E-01 939 19 1.195483E-01 3.386530E+01 1.034633E-01 939 20 1.242673E-01 3.380657E+01 9.927840E-02 939 21 1.289863E-01 3.360622E+01 9.566794E-02 939 22 1.337054E-01 3.343265E+01 9.248375E-02 939 23 1.384244E-01 3.322985E+01 8.957159E-02 939 24 1.431434E-01 3.305530E+01 8.667263E-02 939 25 1.478625E-01 3.276358E+01 8.424020E-02 939 26 1.525815E-01 3.246485E+01 8.195781E-02 939 27 1.573005E-01 3.224972E+01 7.990006E-02 939 28 1.620196E-01 3.198538E+01 7.774151E-02 939 29 1.667386E-01 3.178265E+01 7.603775E-02 939 30 1.714576E-01 3.152022E+01 7.448178E-02 939 31 1.761767E-01 3.114359E+01 7.280674E-02 939 32 1.808957E-01 3.089406E+01 7.116011E-02 939 33 1.856147E-01 3.069395E+01 6.980357E-02 939 34 1.903338E-01 3.030510E+01 6.841354E-02 939 35 1.950528E-01 3.009011E+01 6.719433E-02 939 36 1.997718E-01 2.979656E+01 6.584010E-02 939 37 2.044909E-01 2.961927E+01 6.477067E-02 939 38 2.092099E-01 2.928456E+01 6.362536E-02 939 39 2.139289E-01 2.898044E+01 6.254241E-02 939 40 2.186480E-01 2.865596E+01 6.139990E-02 939 41 2.233670E-01 2.840198E+01 6.041719E-02 939 42 2.280860E-01 2.809246E+01 5.944071E-02 939 43 2.328051E-01 2.792090E+01 5.861123E-02 939 44 2.375241E-01 2.754779E+01 5.767856E-02 939 45 2.422431E-01 2.716256E+01 5.669959E-02 939 46 2.469622E-01 2.691769E+01 5.594271E-02 939 47 2.516812E-01 2.667970E+01 5.514638E-02 939 48 2.564002E-01 2.640198E+01 5.435067E-02 939 49 2.611193E-01 2.611187E+01 5.352513E-02 939 50 2.658383E-01 2.577542E+01 5.276466E-02 939 51 2.705573E-01 2.549986E+01 5.207811E-02 939 52 2.752764E-01 2.521284E+01 5.136601E-02 939 53 2.799954E-01 2.499669E+01 5.066720E-02 939 54 2.847144E-01 2.464570E+01 4.996700E-02 939 55 2.894335E-01 2.428843E+01 4.925646E-02 939 56 2.941525E-01 2.404826E+01 4.863709E-02 939 57 2.988715E-01 2.382412E+01 4.800571E-02 939 58 3.035906E-01 2.350378E+01 4.741837E-02 939 59 3.083096E-01 2.313058E+01 4.679795E-02 939 60 3.130286E-01 2.286377E+01 4.623220E-02 939 61 3.177477E-01 2.264954E+01 4.564747E-02 939 62 3.224667E-01 2.245077E+01 4.517062E-02 939 63 3.271857E-01 2.228072E+01 4.510502E-02 939 64 3.319048E-01 2.190331E+01 4.541705E-02 939 65 3.366238E-01 2.159829E+01 4.534859E-02 939 66 3.413428E-01 2.135966E+01 4.524385E-02 939 67 3.460619E-01 2.115607E+01 4.551393E-02 939 68 3.507809E-01 2.085764E+01 4.633117E-02 939 69 3.554999E-01 2.055184E+01 4.669363E-02 939 70 3.602190E-01 2.027601E+01 4.690776E-02 939 71 3.649380E-01 2.000694E+01 4.706373E-02 939 72 3.696570E-01 1.981029E+01 4.730148E-02 939 73 3.743761E-01 1.956747E+01 4.738133E-02 939 74 3.790951E-01 1.932859E+01 4.745241E-02 939 75 3.838141E-01 1.904442E+01 4.746345E-02 939 76 3.885332E-01 1.886891E+01 4.748032E-02 939 77 3.932522E-01 1.869717E+01 4.763076E-02 939 78 3.979712E-01 1.843426E+01 4.897639E-02 939 79 4.026903E-01 1.816839E+01 5.000441E-02 939 80 4.074093E-01 1.800764E+01 5.083202E-02 939 81 4.121283E-01 1.770449E+01 5.142202E-02 939 82 4.168474E-01 1.742170E+01 5.202169E-02 939 83 4.215664E-01 1.718470E+01 5.251908E-02 939 84 4.262854E-01 1.695241E+01 5.304205E-02 939 85 4.310045E-01 1.675017E+01 5.363423E-02 939 86 4.357235E-01 1.661306E+01 5.430781E-02 939 87 4.404425E-01 1.638750E+01 5.486531E-02 939 88 4.451616E-01 1.608659E+01 5.530137E-02 939 89 4.498806E-01 1.592065E+01 5.560489E-02 939 90 4.545997E-01 1.570057E+01 5.576567E-02 939 91 4.593187E-01 1.552947E+01 5.544231E-02 939 92 4.640377E-01 1.547111E+01 5.330330E-02 939 93 4.687568E-01 1.520343E+01 5.190696E-02 939 94 4.734758E-01 1.501797E+01 5.097865E-02 939 95 4.781948E-01 1.487507E+01 5.029056E-02 939 96 4.829139E-01 1.466401E+01 4.966087E-02 939 97 4.876329E-01 1.448690E+01 4.911472E-02 939 98 4.923519E-01 1.426653E+01 4.858735E-02 939 99 4.970710E-01 1.413102E+01 4.813342E-02 939 100 5.017900E-01 1.393822E+01 4.766874E-02 939 101 5.065090E-01 1.377815E+01 4.729600E-02 939 102 5.112281E-01 1.362602E+01 4.686039E-02 939 103 5.159471E-01 1.347288E+01 4.636475E-02 939 104 5.206661E-01 1.326876E+01 4.581347E-02 939 105 5.253852E-01 1.313039E+01 4.533076E-02 939 106 5.301042E-01 1.301520E+01 4.485715E-02 939 107 5.348232E-01 1.279389E+01 4.432660E-02 939 108 5.395423E-01 1.266336E+01 4.391386E-02 939 109 5.442613E-01 1.253694E+01 4.348388E-02 939 110 5.489803E-01 1.241375E+01 4.306410E-02 939 111 5.536994E-01 1.228730E+01 4.264475E-02 939 112 5.584184E-01 1.213938E+01 4.226984E-02 939 113 5.631374E-01 1.201680E+01 4.190161E-02 939 114 5.678565E-01 1.184680E+01 4.157887E-02 939 115 5.725755E-01 1.170808E+01 4.131196E-02 939 116 5.772945E-01 1.152518E+01 4.104790E-02 939 117 5.820136E-01 1.149164E+01 4.087396E-02 939 118 5.867326E-01 1.136064E+01 4.064059E-02 939 119 5.914516E-01 1.120899E+01 4.038766E-02 939 120 5.961707E-01 1.101709E+01 4.010914E-02 939 121 6.008897E-01 1.091975E+01 3.994597E-02 939 122 6.056087E-01 1.082368E+01 3.974638E-02 939 123 6.103278E-01 1.071830E+01 3.948191E-02 939 124 6.150468E-01 1.056267E+01 3.927580E-02 939 125 6.197658E-01 1.046767E+01 3.914717E-02 939 126 6.244849E-01 1.035416E+01 3.892728E-02 939 127 6.292039E-01 1.024550E+01 3.867703E-02 939 128 6.339229E-01 1.016107E+01 3.853368E-02 939 129 6.386420E-01 1.005288E+01 3.830862E-02 939 130 6.433610E-01 9.901907E+00 3.806817E-02 939 131 6.480800E-01 9.773328E+00 3.783560E-02 939 132 6.527991E-01 9.725771E+00 3.771400E-02 939 133 6.575181E-01 9.585669E+00 3.746850E-02 939 134 6.622371E-01 9.478748E+00 3.728589E-02 939 135 6.669562E-01 9.344014E+00 3.707804E-02 939 136 6.716752E-01 9.285438E+00 3.692526E-02 939 137 6.763942E-01 9.185549E+00 3.670777E-02 939 138 6.811133E-01 9.097495E+00 3.657002E-02 939 139 6.858323E-01 9.060581E+00 3.642270E-02 939 140 6.905513E-01 8.955807E+00 3.621812E-02 939 141 6.952704E-01 8.851378E+00 3.606766E-02 939 142 6.999894E-01 8.790264E+00 3.590878E-02 939 143 7.047084E-01 8.655978E+00 3.571832E-02 939 144 7.094275E-01 8.568818E+00 3.553717E-02 939 145 7.141465E-01 8.461408E+00 3.533544E-02 939 146 7.188655E-01 8.414844E+00 3.522521E-02 939 147 7.235846E-01 8.320146E+00 3.504088E-02 939 148 7.283036E-01 8.196681E+00 3.485406E-02 939 149 7.330226E-01 8.111689E+00 3.467661E-02 939 150 7.377417E-01 8.019353E+00 3.451188E-02 939 151 7.424607E-01 7.987343E+00 3.441920E-02 939 152 7.471797E-01 7.855709E+00 3.419690E-02 939 153 7.518988E-01 7.747117E+00 3.401441E-02 939 154 7.566178E-01 7.708594E+00 3.391035E-02 939 155 7.613368E-01 7.628857E+00 3.376998E-02 939 156 7.660559E-01 7.537997E+00 3.357371E-02 939 157 7.707749E-01 7.452939E+00 3.342167E-02 939 158 7.754939E-01 7.338198E+00 3.325884E-02 939 159 7.802130E-01 7.285337E+00 3.312774E-02 939 160 7.849320E-01 7.235159E+00 3.300671E-02 939 161 7.896510E-01 7.166433E+00 3.285964E-02 939 162 7.943701E-01 7.009006E+00 3.265180E-02 939 163 7.990891E-01 7.009505E+00 3.260443E-02 939 164 8.038082E-01 6.902261E+00 3.242123E-02 939 165 8.085272E-01 6.867402E+00 3.227001E-02 939 166 8.132462E-01 6.765913E+00 3.212383E-02 939 167 8.179653E-01 6.687609E+00 3.199066E-02 939 168 8.226843E-01 6.577363E+00 3.179424E-02 939 169 8.274033E-01 6.523515E+00 3.168836E-02 939 170 8.321224E-01 6.474791E+00 3.158369E-02 939 171 8.368414E-01 6.369889E+00 3.142489E-02 939 172 8.415604E-01 6.261080E+00 3.119801E-02 939 173 8.462795E-01 6.227948E+00 3.108274E-02 939 174 8.509985E-01 6.173503E+00 3.100302E-02 939 175 8.557175E-01 6.133523E+00 3.090228E-02 939 176 8.604366E-01 6.022871E+00 3.073464E-02 939 177 8.651556E-01 5.965108E+00 3.059842E-02 939 178 8.698746E-01 5.898694E+00 3.050288E-02 939 179 8.745937E-01 5.839950E+00 3.035337E-02 939 180 8.793127E-01 5.728398E+00 3.018755E-02 939 181 8.840317E-01 5.668533E+00 3.004640E-02 939 182 8.887508E-01 5.647506E+00 2.997377E-02 939 183 8.934698E-01 5.569974E+00 2.985527E-02 939 184 8.981888E-01 5.474292E+00 2.966729E-02 939 185 9.029079E-01 5.401620E+00 2.953940E-02 939 186 9.076269E-01 5.408386E+00 2.947798E-02 939 187 9.123459E-01 5.309438E+00 2.932994E-02 939 188 9.170650E-01 5.210955E+00 2.917623E-02 939 189 9.217840E-01 5.157289E+00 2.908806E-02 939 190 9.265030E-01 5.065059E+00 2.893898E-02 939 191 9.312221E-01 4.989118E+00 2.878714E-02 939 192 9.359411E-01 4.964460E+00 2.871521E-02 939 193 9.406601E-01 4.931948E+00 2.859766E-02 939 194 9.453792E-01 4.833463E+00 2.845759E-02 939 195 9.500982E-01 4.798913E+00 2.835903E-02 939 196 9.548172E-01 4.713110E+00 2.822836E-02 939 197 9.595363E-01 4.666203E+00 2.812507E-02 939 198 9.642553E-01 4.578968E+00 2.798270E-02 939 199 9.689743E-01 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