{
  "code": "SASDEC8",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.",
    "publication": {
      "title": "Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.",
      "author_list": "Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ",
      "journal": "Structure",
      "doi": "10.1016/j.str.2019.03.019",
      "pmid": "31056420",
      "published_date": "2019 Jul 2"
    },
    "status": "released",
    "submitted_date": "2018-11-23",
    "released_date": "2019-05-06"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDEC8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDEC8.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Hybrid Photon Counting (HPC) X-ray detector",
        "name": "Rigaku HyPix-3000",
        "resolution": 100.0
      },
      "name": "University of British Columbia",
      "city": "Vancouver",
      "country": "Canada",
      "beamline_name": "Rigaku BioSAXS-2000",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Bacillus thuringiensis LexA repressor",
          "short_name": "LexA",
          "sequence": "MLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYI\r\nRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVSGA\r\nDQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFR\r\nLQPENSSLEPIILKQVSVIGKVIGVYRDLH",
          "organism": "Bacillus thuringiensis",
          "uniprot_code": "A0A2B4EEI3",
          "uniprot_range_first": 1,
          "uniprot_range_last": 210,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYI\nRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVSGA\nDQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFR\nLQPENSSLEPIILKQVSVIGKVIGVYRDLH",
          "mw": 23.394,
          "total_mw": 46.788,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Bacteriophage pGIL01 gp7",
          "short_name": "gp7",
          "sequence": "MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKNVEGKKDD",
          "organism": "Bacteriophage pGIL01",
          "uniprot_code": "Q7WSG2",
          "uniprot_range_first": 1,
          "uniprot_range_last": 50,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKNVEGKKDD",
          "mw": 5.921,
          "total_mw": 23.684,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Hepes, 300 mM NaCl, 10% glycerol,",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Vancouver, Canada",
            "full_name": "University of British Columbia",
            "webpage": "https://www.ubc.ca/"
          }
        ],
        "contributor_name": "Nathanael",
        "contributor_surname": "Caveney",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-08-25",
    "storage_temperature": 6.0,
    "cell_temperature": 6.0,
    "exposure_time": 300.0,
    "number_of_frames": 12,
    "wavelength": 0.154,
    "sample_detector_distance": null,
    "concentration_min": 0.75,
    "concentration_max": 12.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.117,
    "s_max": 6.422,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit1_model1_img.png",
          "software": "Chimera",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDEC8_fit1_model1.pdb",
          "model_mw": 59.42,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex",
          "user": 389
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/LexA_gp7_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 6.158,
      "p_value": 0.0,
      "fit_residual_plot": "LexA_gp7_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDEC8_fit1.fit",
      "fit_log": null,
      "software_version": null,
      "description": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex"
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit2_model1_img.png",
          "software": "Chimera",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDEC8_fit2_model1.pdb",
          "model_mw": 59.42,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, restrained",
          "user": 389
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/rc01_LgiSRAn_fit_img.png",
      "software": "SREFLEX",
      "chi_square_value": 2.58,
      "p_value": 2e-06,
      "fit_residual_plot": "rc01_LgiSRAn_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDEC8_fit2.fit",
      "fit_log": null,
      "software_version": null,
      "description": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, restrained"
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit3_model1_img.png",
          "software": "Chimera",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDEC8_fit3_model1.pdb",
          "model_mw": 59.42,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, unrestrained",
          "user": 389
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/uc01_m3mT2gz_fit_img.png",
      "software": "SREFLEX",
      "chi_square_value": 1.899,
      "p_value": 4e-05,
      "fit_residual_plot": "uc01_m3mT2gz_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDEC8_fit3.fit",
      "fit_log": null,
      "software_version": null,
      "description": "I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, unrestrained"
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": null,
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SAXS data from solutions of Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7) in 20 mM HEPES, 300 mM NaCl, 10% glycerol, pH 8 were collected on a Rigaku BioSAXS-2000 instrument at the University of British Columbia (Vancouver, Canada) using a Hybrid Pixel Array Detector Rigaku HyOix-3000 detector at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 12 mg/ml were measured at 6°C. 12 successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 108.2,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
  "guinier_i0": 1.53,
  "guinier_i0_error": null,
  "pddf_rg": null,
  "pddf_rg_error": null,
  "guinier_rg": 4.391,
  "guinier_rg_error": null,
  "pddf_dmax": null,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": null,
  "guinier_point_last": null,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDEC8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}