data_SASDFC8_MAIN # _sas_result.id 1483 _sas_result.SASBDB_code SASDFC8 _sas_result.experimental_MW 33.100 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 46.000 _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 4203.0000000000 _sas_result.I0_from_Guinier_error 29.6000000000 _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 4.490 _sas_result.Rg_from_Guinier_error 0.530 _sas_result.D_max 21.00 _sas_result.Porod_volume 74.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 28 _sas_result.Guinier_point_max 133 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl" _sas_scan.detc_id 4 _sas_scan.id 1483 _sas_scan.measurement_date 2016-10-03 _sas_scan.storage_temperature 20.0 _sas_scan.cell_temperature 20.0 _sas_scan.exposure_time 0.050 _sas_scan.number_of_frames 20 _sas_scan.unit 1/nm _sas_scan.qmin 0.0134 _sas_scan.qmax 3.7904 _sas_scan.sample_id 1444 _sas_scan.result_id 1483 _sas_scan.beam_id 6 # _sas_beam.id 6 _sas_beam.instrument_name "PETRA III EMBL P12" _sas_beam.instrument_city Hamburg _sas_beam.instrument_country Germany _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.124000 _sas_beam.dist_spec_to_detc 3.00000 # _sas_detc.id 4 _sas_detc.name "Pilatus 2M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1444 _sas_sample.name "Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.60 _sas_sample.concentration_method . _sas_sample.entity_id 1738 # _sas_buffer.id 900 _sas_buffer.name "20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 6.600 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1444 # _entity.id 1738 _entity.type protein _entity.pdbx_description "DNA-directed RNA polymerase subunit delta - mutant" _entity.formula_weight 20.405 _entity.pdbx_number_of_molecules 1 # _entity_name_com.entity_id 1738 _entity_name_com.name "rpoE KE mutant" # _entity_poly.entity_id 1738 _entity_poly.pdbx_seq_one_letter_code MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVEAEEEEAEEAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK # _entity_src_gen.entity_id 1738 _entity_src_gen.gene_src_common_name "Bacillus subtilis (strain 168)" # _struct_ref.id 1 _struct_ref.entity_id 1738 _struct_ref.db_name UniProt _struct_ref.db_code P12464 _struct_ref.details http://www.uniprot.org/uniprot/P12464 # _citation.id 495 _citation.title "Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase." _citation.year "2019 Sep 24" _citation.pdbx_database_id_DOI 10.1021/jacs.9b07837 _citation.pdbx_database_id_PubMed 31550880 # loop_ _citation_author.citation_id _citation_author.ordinal _citation_author.name 495 1 "Kuban V" 495 2 "Srb P" 495 3 "Stegnerova H" 495 4 "Padrta P" 495 5 "Zachrdla M" 495 6 "Jasenakova Z" 495 7 "Šanderová H" 495 8 "Vítovská D" 495 9 "Krasny L" 495 10 "Koval T" 495 11 "Dohnalek J" 495 12 "Ziemska-Legi Cka J" 495 13 "Grynberg M" 495 14 "Jarnot P" 495 15 "Gruca A" 495 16 "Jensen MR" 495 17 "Blackledge M" # _pdbx_contact_author.id 83 _pdbx_contact_author.name_first Vojtěch _pdbx_contact_author.name_last Kubáň _pdbx_contact_author.role . _pdbx_contact_author.address_1 MUNI _pdbx_contact_author.address_2 "Masaryk University; Research group Structure and Dynamics of Proteins." _pdbx_contact_author.address_3 "Brno, Czech Republic" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 1.337960E-02 -1.763300E+04 9.882605E+03 1483 2 1.546980E-02 1.793600E+04 6.366085E+03 1483 3 1.756010E-02 1.057600E+04 3.963866E+03 1483 4 1.965030E-02 1.134670E+04 2.676380E+03 1483 5 2.174050E-02 8.274900E+03 2.183528E+03 1483 6 2.383070E-02 8.025600E+03 1.714432E+03 1483 7 2.592090E-02 7.749300E+03 1.526213E+03 1483 8 2.801120E-02 7.130700E+03 1.175303E+03 1483 9 3.010140E-02 4.416400E+03 1.018096E+03 1483 10 3.219160E-02 5.863390E+03 8.897646E+02 1483 11 3.428180E-02 3.897410E+03 7.458455E+02 1483 12 3.637210E-02 5.431450E+03 6.905065E+02 1483 13 3.846230E-02 4.949790E+03 5.699962E+02 1483 14 4.055250E-02 6.046910E+03 5.281294E+02 1483 15 4.264270E-02 4.643830E+03 4.775130E+02 1483 16 4.473290E-02 4.726470E+03 4.253275E+02 1483 17 4.682320E-02 4.883740E+03 4.066226E+02 1483 18 4.891340E-02 4.378780E+03 3.738062E+02 1483 19 5.100360E-02 4.512070E+03 3.703994E+02 1483 20 5.309380E-02 4.609930E+03 3.438307E+02 1483 21 5.518410E-02 4.421890E+03 3.198878E+02 1483 22 5.727430E-02 4.692880E+03 3.169537E+02 1483 23 5.936450E-02 4.513870E+03 2.937063E+02 1483 24 6.145470E-02 4.226110E+03 2.943150E+02 1483 25 6.354490E-02 4.698038E+03 2.666814E+02 1483 26 6.563520E-02 4.520592E+03 2.629489E+02 1483 27 6.772540E-02 4.708670E+03 2.573860E+02 1483 28 6.981560E-02 4.157825E+03 2.462905E+02 1483 29 7.190580E-02 4.126027E+03 2.284654E+02 1483 30 7.399610E-02 4.350603E+03 2.250987E+02 1483 31 7.608630E-02 4.012304E+03 2.258363E+02 1483 32 7.817650E-02 4.284252E+03 2.180301E+02 1483 33 8.026670E-02 4.019367E+03 2.096089E+02 1483 34 8.235690E-02 4.032754E+03 2.021656E+02 1483 35 8.444720E-02 3.827536E+03 1.974604E+02 1483 36 8.653740E-02 3.971576E+03 1.977924E+02 1483 37 8.862760E-02 4.332160E+03 1.922590E+02 1483 38 9.071780E-02 3.765848E+03 1.845531E+02 1483 39 9.280810E-02 4.004348E+03 1.815575E+02 1483 40 9.489830E-02 4.254498E+03 1.871935E+02 1483 41 9.698850E-02 3.973919E+03 1.816456E+02 1483 42 9.907870E-02 3.861948E+03 1.699542E+02 1483 43 1.011690E-01 4.171317E+03 1.751804E+02 1483 44 1.032590E-01 3.961205E+03 1.675571E+02 1483 45 1.053490E-01 3.771229E+03 1.688531E+02 1483 46 1.074400E-01 3.906213E+03 1.618742E+02 1483 47 1.095300E-01 3.926189E+03 1.604084E+02 1483 48 1.116200E-01 4.134898E+03 1.686256E+02 1483 49 1.137100E-01 3.950815E+03 1.575304E+02 1483 50 1.158000E-01 3.847750E+03 1.548030E+02 1483 51 1.178910E-01 3.683563E+03 1.526049E+02 1483 52 1.199810E-01 3.795969E+03 1.514974E+02 1483 53 1.220710E-01 3.696027E+03 1.543383E+02 1483 54 1.241610E-01 3.898524E+03 1.436954E+02 1483 55 1.262520E-01 3.621239E+03 1.449562E+02 1483 56 1.283420E-01 3.674052E+03 1.447997E+02 1483 57 1.304320E-01 3.636663E+03 1.435854E+02 1483 58 1.325220E-01 3.442850E+03 1.411975E+02 1483 59 1.346120E-01 3.938282E+03 1.394994E+02 1483 60 1.367030E-01 3.649006E+03 1.392358E+02 1483 61 1.387930E-01 4.129263E+03 1.395893E+02 1483 62 1.408830E-01 3.688949E+03 1.378034E+02 1483 63 1.429730E-01 3.814632E+03 1.318502E+02 1483 64 1.450640E-01 3.700993E+03 1.338535E+02 1483 65 1.471540E-01 3.558800E+03 1.348669E+02 1483 66 1.492440E-01 3.528227E+03 1.295307E+02 1483 67 1.513340E-01 3.688872E+03 1.299705E+02 1483 68 1.534240E-01 3.594706E+03 1.277582E+02 1483 69 1.555150E-01 3.587621E+03 1.276406E+02 1483 70 1.576050E-01 3.499094E+03 1.278181E+02 1483 71 1.596950E-01 3.197753E+03 1.223185E+02 1483 72 1.617850E-01 3.590816E+03 1.252324E+02 1483 73 1.638760E-01 3.595908E+03 1.223725E+02 1483 74 1.659660E-01 3.320600E+03 1.219558E+02 1483 75 1.680560E-01 3.397171E+03 1.201953E+02 1483 76 1.701460E-01 3.373381E+03 1.180470E+02 1483 77 1.722360E-01 3.612574E+03 1.228393E+02 1483 78 1.743270E-01 3.416614E+03 1.170718E+02 1483 79 1.764170E-01 3.270506E+03 1.172597E+02 1483 80 1.785070E-01 3.406783E+03 1.130270E+02 1483 81 1.805970E-01 3.254246E+03 1.148743E+02 1483 82 1.826880E-01 3.228075E+03 1.161527E+02 1483 83 1.847780E-01 3.231299E+03 1.126298E+02 1483 84 1.868680E-01 3.307728E+03 1.128404E+02 1483 85 1.889580E-01 3.187463E+03 1.102299E+02 1483 86 1.910480E-01 3.105205E+03 1.098117E+02 1483 87 1.931390E-01 3.161719E+03 1.120306E+02 1483 88 1.952290E-01 3.243591E+03 1.082791E+02 1483 89 1.973190E-01 3.340944E+03 1.118399E+02 1483 90 1.994090E-01 3.272328E+03 1.074346E+02 1483 91 2.015000E-01 3.214507E+03 1.084399E+02 1483 92 2.035900E-01 3.075951E+03 1.065751E+02 1483 93 2.056800E-01 3.033737E+03 1.045722E+02 1483 94 2.077700E-01 3.205689E+03 1.079936E+02 1483 95 2.098600E-01 3.336447E+03 1.041069E+02 1483 96 2.119510E-01 3.045391E+03 1.056245E+02 1483 97 2.140410E-01 3.166751E+03 1.016470E+02 1483 98 2.161310E-01 2.917609E+03 1.008761E+02 1483 99 2.182210E-01 2.929008E+03 1.036300E+02 1483 100 2.203120E-01 3.129891E+03 1.020799E+02 1483 101 2.224020E-01 3.114913E+03 1.015483E+02 1483 102 2.244920E-01 3.083512E+03 1.003489E+02 1483 103 2.265820E-01 2.969007E+03 9.831074E+01 1483 104 2.286720E-01 2.788551E+03 9.856183E+01 1483 105 2.307630E-01 3.091851E+03 9.888326E+01 1483 106 2.328530E-01 2.912088E+03 9.790006E+01 1483 107 2.349430E-01 2.934605E+03 9.749328E+01 1483 108 2.370330E-01 2.866818E+03 9.818712E+01 1483 109 2.391240E-01 2.792917E+03 9.599530E+01 1483 110 2.412140E-01 2.823364E+03 9.452022E+01 1483 111 2.433040E-01 2.959914E+03 9.702593E+01 1483 112 2.453940E-01 2.641383E+03 9.348713E+01 1483 113 2.474840E-01 2.588075E+03 9.406013E+01 1483 114 2.495750E-01 2.657585E+03 9.141795E+01 1483 115 2.516650E-01 2.628123E+03 9.279159E+01 1483 116 2.537550E-01 2.466051E+03 9.084519E+01 1483 117 2.558450E-01 2.668510E+03 9.184079E+01 1483 118 2.579360E-01 2.717631E+03 9.079953E+01 1483 119 2.600260E-01 2.781995E+03 9.131658E+01 1483 120 2.621160E-01 2.754419E+03 9.186291E+01 1483 121 2.642060E-01 2.628363E+03 9.029581E+01 1483 122 2.662960E-01 2.499982E+03 8.837552E+01 1483 123 2.683870E-01 2.668596E+03 8.894969E+01 1483 124 2.704770E-01 2.547031E+03 8.680861E+01 1483 125 2.725670E-01 2.787739E+03 9.017819E+01 1483 126 2.746570E-01 2.579479E+03 8.801952E+01 1483 127 2.767480E-01 2.553623E+03 8.693434E+01 1483 128 2.788380E-01 2.421616E+03 8.665356E+01 1483 129 2.809280E-01 2.653823E+03 8.648929E+01 1483 130 2.830180E-01 2.562709E+03 8.677831E+01 1483 131 2.851080E-01 2.385560E+03 8.427371E+01 1483 132 2.871990E-01 2.425479E+03 8.592982E+01 1483 133 2.892890E-01 2.422416E+03 8.447560E+01 1483 134 2.913790E-01 2.399321E+03 8.400802E+01 1483 135 2.934690E-01 2.584940E+03 8.454949E+01 1483 136 2.955600E-01 2.393709E+03 8.134735E+01 1483 137 2.976500E-01 2.283060E+03 8.412624E+01 1483 138 2.997400E-01 2.351089E+03 8.234021E+01 1483 139 3.018300E-01 2.403527E+03 8.246894E+01 1483 140 3.039200E-01 2.422105E+03 8.267108E+01 1483 141 3.060110E-01 2.378867E+03 8.082062E+01 1483 142 3.081010E-01 2.250334E+03 8.275724E+01 1483 143 3.101910E-01 2.303649E+03 7.996714E+01 1483 144 3.122810E-01 2.190609E+03 7.976531E+01 1483 145 3.143720E-01 2.310748E+03 8.076009E+01 1483 146 3.164620E-01 2.299317E+03 7.987379E+01 1483 147 3.185520E-01 2.274274E+03 8.036274E+01 1483 148 3.206420E-01 2.183511E+03 7.687065E+01 1483 149 3.227320E-01 2.111943E+03 7.877656E+01 1483 150 3.248230E-01 2.310821E+03 7.924085E+01 1483 151 3.269130E-01 2.181012E+03 7.793578E+01 1483 152 3.290030E-01 2.328616E+03 8.086221E+01 1483 153 3.310930E-01 2.139684E+03 7.564675E+01 1483 154 3.331840E-01 2.150907E+03 7.799079E+01 1483 155 3.352740E-01 2.101190E+03 7.693733E+01 1483 156 3.373640E-01 2.141770E+03 7.612856E+01 1483 157 3.394540E-01 2.107861E+03 7.648709E+01 1483 158 3.415440E-01 1.943938E+03 7.492986E+01 1483 159 3.436350E-01 2.006818E+03 7.618149E+01 1483 160 3.457250E-01 2.099813E+03 7.503331E+01 1483 161 3.478150E-01 2.065881E+03 7.539353E+01 1483 162 3.499050E-01 2.013941E+03 7.822816E+01 1483 163 3.519960E-01 1.972693E+03 7.931633E+01 1483 164 3.540860E-01 2.034376E+03 8.156654E+01 1483 165 3.561760E-01 1.900657E+03 7.932961E+01 1483 166 3.582660E-01 1.994787E+03 8.301047E+01 1483 167 3.603560E-01 1.950617E+03 8.373756E+01 1483 168 3.624470E-01 1.977345E+03 8.514005E+01 1483 169 3.645370E-01 1.920441E+03 8.424084E+01 1483 170 3.666270E-01 1.910633E+03 8.444510E+01 1483 171 3.687170E-01 1.749082E+03 8.623940E+01 1483 172 3.708080E-01 1.944758E+03 8.559184E+01 1483 173 3.728980E-01 1.900649E+03 8.413346E+01 1483 174 3.749880E-01 1.964248E+03 8.497262E+01 1483 175 3.770780E-01 1.851479E+03 8.497893E+01 1483 176 3.791680E-01 1.841390E+03 8.610030E+01 1483 177 3.812590E-01 1.724289E+03 8.110895E+01 1483 178 3.833490E-01 1.810048E+03 8.557101E+01 1483 179 3.854390E-01 1.868326E+03 8.464947E+01 1483 180 3.875290E-01 1.683971E+03 8.458772E+01 1483 181 3.896200E-01 1.899943E+03 8.614938E+01 1483 182 3.917100E-01 1.667915E+03 8.275117E+01 1483 183 3.938000E-01 1.672846E+03 8.289862E+01 1483 184 3.958900E-01 1.648007E+03 7.815386E+01 1483 185 3.979800E-01 1.676811E+03 7.842950E+01 1483 186 4.000710E-01 1.743414E+03 7.736052E+01 1483 187 4.021610E-01 1.622422E+03 7.573321E+01 1483 188 4.042510E-01 1.686462E+03 7.829618E+01 1483 189 4.063410E-01 1.638109E+03 7.483268E+01 1483 190 4.084320E-01 1.797016E+03 7.609880E+01 1483 191 4.105220E-01 1.610264E+03 7.424217E+01 1483 192 4.126120E-01 1.688145E+03 7.310285E+01 1483 193 4.147020E-01 1.663368E+03 7.490044E+01 1483 194 4.167920E-01 1.609354E+03 7.206307E+01 1483 195 4.188830E-01 1.589845E+03 7.241174E+01 1483 196 4.209730E-01 1.562913E+03 7.157021E+01 1483 197 4.230630E-01 1.650119E+03 7.198327E+01 1483 198 4.251530E-01 1.535210E+03 7.156153E+01 1483 199 4.272440E-01 1.595345E+03 7.034646E+01 1483 200 4.293340E-01 1.485337E+03 7.087533E+01 1483 201 4.314240E-01 1.611360E+03 6.939062E+01 1483 202 4.335140E-01 1.671161E+03 7.108540E+01 1483 203 4.356040E-01 1.610175E+03 6.915955E+01 1483 204 4.376950E-01 1.689610E+03 7.033010E+01 1483 205 4.397850E-01 1.551358E+03 6.944847E+01 1483 206 4.418750E-01 1.453949E+03 6.789724E+01 1483 207 4.439650E-01 1.522610E+03 6.829499E+01 1483 208 4.460560E-01 1.514828E+03 6.774086E+01 1483 209 4.481460E-01 1.510499E+03 6.799635E+01 1483 210 4.502360E-01 1.431859E+03 6.851814E+01 1483 211 4.523260E-01 1.527850E+03 6.595742E+01 1483 212 4.544160E-01 1.293760E+03 6.572553E+01 1483 213 4.565070E-01 1.458698E+03 6.698361E+01 1483 214 4.585970E-01 1.443667E+03 6.678294E+01 1483 215 4.606870E-01 1.393863E+03 6.545443E+01 1483 216 4.627770E-01 1.477462E+03 6.530700E+01 1483 217 4.648680E-01 1.412486E+03 6.530073E+01 1483 218 4.669580E-01 1.296108E+03 6.465343E+01 1483 219 4.690480E-01 1.444775E+03 6.556548E+01 1483 220 4.711380E-01 1.244996E+03 6.293218E+01 1483 221 4.732280E-01 1.318711E+03 6.450636E+01 1483 222 4.753190E-01 1.481831E+03 6.477978E+01 1483 223 4.774090E-01 1.379683E+03 6.425900E+01 1483 224 4.794990E-01 1.322862E+03 6.299797E+01 1483 225 4.815890E-01 1.358558E+03 6.312299E+01 1483 226 4.836800E-01 1.451802E+03 6.480741E+01 1483 227 4.857700E-01 1.412903E+03 6.323353E+01 1483 228 4.878600E-01 1.315561E+03 6.248286E+01 1483 229 4.899500E-01 1.394358E+03 6.320535E+01 1483 230 4.920400E-01 1.371018E+03 6.261285E+01 1483 231 4.941310E-01 1.528766E+03 6.331970E+01 1483 232 4.962210E-01 1.298305E+03 6.124202E+01 1483 233 4.983110E-01 1.293322E+03 6.064997E+01 1483 234 5.004010E-01 1.391764E+03 6.199396E+01 1483 235 5.024920E-01 1.368436E+03 6.121473E+01 1483 236 5.045820E-01 1.318566E+03 6.130236E+01 1483 237 5.066720E-01 1.214967E+03 5.973729E+01 1483 238 5.087620E-01 1.359278E+03 6.047501E+01 1483 239 5.108520E-01 1.326796E+03 6.004005E+01 1483 240 5.129430E-01 1.178492E+03 5.919731E+01 1483 241 5.150330E-01 1.315744E+03 5.991460E+01 1483 242 5.171230E-01 1.271166E+03 5.947015E+01 1483 243 5.192130E-01 1.290233E+03 5.981443E+01 1483 244 5.213040E-01 1.241274E+03 5.950686E+01 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