{ "code": "SASD2D2", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "A β(H)-spectrin self-interaction domain forms antiparallel dimers that may facilitate non-canonical spectrin membrane skeleton assembly.", "publication": { "title": "A β(H)-spectrin self-interaction domain forms antiparallel dimers that may facilitate non-canonical spectrin membrane skeleton assembly.", "author_list": "Sarring C, Stoute JV, Armache JP, Yennawar NH, Thomas CM", "journal": "J Biol Chem", "doi": "10.1016/j.jbc.2026.113388", "pmid": "42551825", "published_date": "2026 Aug 4" }, "status": "released", "submitted_date": "2026-06-18", "released_date": "2026-08-02" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASD2D2.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASD2D2.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2D2_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2D2_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASD2D2_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASD2D2_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASD2D2.sascif", "experiment": { "instrument": { "detector": { "type": "Hybrid Photon Counting (HPC) X-ray detector", "name": "Rigaku HyPix-3000", "resolution": 100.0 }, "name": "Pennsylvania State University", "city": "University Park, PA", "country": "USA", "beamline_name": "Rigaku BioSAXS-2000", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Beta-Heavy-Spectrin", "short_name": "Beta[H]", "sequence": "GSRLTQSHQLQEFLRSLYEIDRWLVQKLQVALDENYREPSNLQSKIQKHAAFDAELLSNSPRVQSVIHEGERLIRGDHFAKDEIAQQVQLLEGDWLKLKGASQTKKDKLQQAYDALAFNRSVDEFNNWMDEVELQLSSEDYGKDLAAVSNLLKKHERLEADVAHHGELADQLKQKDEQFFQAEHFLRHEIHERATVSIRRYNTLHEPLGIRRENLEDSLSLQQFLRDAEDELQWLAEKQLVAGSQDLGTSLLSVQGLQKKHNSLEAELTSQEPLIQALLQRGQQMIRDNHFASEQLQYKSELLQKQLVQLRDLAAIRRLRLLDAVESQLFYVEANEADAWMREKRPVLSSSDYGRDEVSVQGHQKKLEVLQRELTAFKPSIEKVAKLATGLIERNHFDSSKIAEKNAQVGQEYEDLLRLAKERESRLGECKKLFEYLRETEELHEWVGDQMAVTASEDYGEDVEHVEQLILAFESFVSNLNANEARVEACLERGDRLIQENNPYRSSIKSKRDETKQLWEELKDLVHARQDALAGAKQVHVYDRVADETIQLINEKDASLISEDYGQDLESIQALGRKHQVFESELVGIQGQVDSVLAEAAKLGEIYPDAKEHIEVKRDETVEAWTDLKEKTAARKNKLSQAEQLQSYFDEYRDLIAWINEMLAKITAPELANSVAGAELLLASTKDHDTEIRTRDETFAKFAANGQQLIKEKHFLAHEVEDKIKVLQARHELLKHTLNKRREIYELNLDT", "organism": "Drosophila melanogaster", "uniprot_code": "Q9VZQ3", "uniprot_range_first": 2773, "uniprot_range_last": 3521, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MTQRDGIIKFENERIKTLQEERLHIQKKTFTKWMNSFLIKAKMEVEDLFTDLADGIKLLK\nLLEIISSEKLGKPNSGRMRVHKIENVNKSLAFLHTKVRLESIGAEDIVDGNPRLILGLIW\nTIILRFQIQEIEIDVDEENESSEKRSAKDALLLWCQRKTHGYPGVNITDFTNSWRSGLGF\nNALIHSHRPDLFEYSTIVNSKNSNLDNLNHAFDTAANELGIPSLLDAEDIDSARPDEKSI\nLTYVASYYHTFARMKNEQKSGKRIANIVGQLMDADRKKMQYEGLTTNLLSWIRQKTLELE\nQRDLPNSLEGIQRELLAFKEYRTIEKPPKYKERSEIEALYFTINTLLKALNQPPYNPQDG\nQLVNDIEKAWQILEYAEHHREVALRDELLRQEKLEQLNYKFEKKSVLREGYLKEMIQVLS\nDPRYLRQVDATLKKHEAISADILARVERFNDLTAMAEELDRENYHGKERVRRREQEVMAK\nWRQLLELLENQRLNLSQMSNLMNLLREIASTTEAVRELQQQFASEDVGPHLLGVEELLQA\nHSLQELQVNTYGETLKRFNRQALPYKSSEHKDAALLAQRLADLEEAYSELLRRSAARRAR\nLEEARNFHHFMEDYDNEESWLVDKQRICKTGITAKDLRAVLSLQQKHKALEDEIKSRKPK\nSGQMSTAGKRLIGEQHPRSSEIQSRIDSLAEHWQALEALVELRRRQLEDAAEAYQFYTDA\nNEAESWLNEKIALVNSRDYGNDEPSAQALLQRHRDLQGELNAYSGDILNLNQQADKLIKA\nGICTLELSAAEPELPEVEQEEWVNETRLVPKEVWEDEWVEKLEHKKVTETKMLPHVKSLF\nPFEGQGMKMDKGEVMLLKSKTNDDWWCVRKDNGVEGFVPANYVREVEPRPVACIVPKAEK\nVKSLQKVKKTILVRQVVPVKRIKPVSVAPKPLVQRRTSTQSINENADSVEKRQQRINQTY\nDELQEMAQKRHALLEDSIHLFGFYRECDDFEKWMKEKERMIKSDEGEGVDNAKRKFEKFI\nTDLSAASKRVEEIDGAVDTFRRQGHSQLDKIIARQRQIHQIWQRLNNAKAQREKSLEGAS\nSVELFNRTCDEAKVWMSEKMLQLDTAVITPDLRTVQALQRRHQNLERELAPVEDKVNRVT\nYLGNSVKNAYPAEKDNVNARQQEVQDMWQQVQQRGSDLRNRIESEVGQQVFNNSAKVLLA\nWIDSVKDQLNADESARDVETANNLLKKHNDLGDDIRAHDTEFVEVIQLGKQLSDGKPNMA\nETVAVIERLKAEQDAIHRGWAEKQKWLLQCVDLQMFNREADKIDATTKSHEAFLEYNNLG\nASLDEVEAILKRHLDFEKSLMAQDKILKGFSDNADKLISNDHYDSKYIGDRRNQVLGKRK\nAVKDRAFERKRLLQASKDFHKFAAEADDLKVWLQDKTRIAGDENYRDLSNLPRKLQKHQA\nFERELRANEGQLRNVTKDGQALVQAGNRVPEVESRVADLNKRWKDLLTLSEDKGRKLEQA\nASQREHNRSLEDAKKKVDELDSALRSGDVGNDLRSCKDLINKQQILESEITIWDQKVAEL\nVSTGDDMAHGGHFNAQNIEAGTKELQQRFKDLRDPTQRRRAKLEESLNYHKFVFELDSEF\nQWINEHLPAAKSNELGQNLHQAQSLHKKHKKLEAEIKGHQPMINKALVAGQSLISQQHPE\nREQVESLCQQLEQAWQDLERHCGERSRKLDMSLKAQQYLFDAGEIESWLGERNNVLRSTE\nYGRDRDSAAKLLTKHKTIELELDTYSGIVTEMGHSCAAMVAANHPDSKVLAAKQQLIEKM\nLKSLHKLASQRQGRLMESLYKHEYFLESDEVEQWIREQEQAASSEDYGQDFEHLQLLQNK\nFDDLKHRVEVGADRVDQCELLAKKLIDSESPYANEVEKRQEQLRTSWENLLQLLNQREQK\nLHAAGEIHRFHRDVAEALFRIQDKNAALSQELGRDLNSALALLRKHEGFENDLVALEAQL\nQVLVEDSVRLQAKYPSNASAIAQQQDKVVAAWNDLKERSTARGDRLAASSDLQTFLTDVR\nDIVSWSSNLRAALQAEEHVSDAAGATALKIQHDAIYGEIEAREDKFRYLNELSDSMVQTG\nHYAAADVEEKCAAMLDERQKLHAAWNKKKIMLEQKIDLFCFLRDAKQIDNLSSSQQAALS\nSSDFGQTVEDVQNKIRKHDEFERLIQTQEEKVSLLQEHGRKLIEQRHYDSANIQTILQGV\nLARRQKVKDLCAVRRYKLEDALLYAKFVRDCAEAKYWINEKQKKLEADAASYAEVTNLDE\nKIKKLQKHQAFQAEVAANQGRIQEIQDTGVILLSKQHESSPEIKRAIEIVLEAWQGLLAE\nLEQRGRGLEEAQDSLEFNSQLDKIEAWIRDKEMMVQASDTGRDLEHCNALMRKLDDVDSD\nMRVDDQRVKHINQLADKLINQAQVPADTQSVDKRRKDFNYNWRQLQGALNAYRALLGGAN\nEIHVFNRDVDDTADRIAEKSLAMSSTDTGRDLAAVEALIRREEALERDMSAVKQKIDQHE\nTAAEFLIKKYPERGAQHIERKLEELHKSWGNLQALSVKRQSILNEAYLAHKFVSDVKELE\nLWVNDMIKKMNNTQSPSTINDCETQLELHQERKVEIEGRQEAFAGLKQQGEQLSKRPQQQ\nQPDNVRKYLLVLEELHQTLNEAWSERARDLTEAHQLQLFKAQVEQVEIWLANKEAFLNND\nDLGDSYTAVERLLKKHDEFEKLLHADHVDTLQKFANSILEGEPKDADLIREKLAYILRRK\nQKLLELSEERKQRLTQSHQLQEFLRSLYEIDRWLVQKLQVALDENYREPSNLQSKIQKHA\nAFDAELLSNSPRVQSVIHEGERLIRGDHFAKDEIAQQVQLLEGDWLKLKGASQTKKDKLQ\nQAYDALAFNRSVDEFNNWMDEVELQLSSEDYGKDLAAVSNLLKKHERLEADVAHHGELAD\nQLKQKDEQFFQAEHFLRHEIHERATVSIRRYNTLHEPLGIRRENLEDSLSLQQFLRDAED\nELQWLAEKQLVAGSQDLGTSLLSVQGLQKKHNSLEAELTSQEPLIQALLQRGQQMIRDNH\nFASEQLQYKSELLQKQLVQLRDLAAIRRLRLLDAVESQLFYVEANEADAWMREKRPVLSS\nSDYGRDEVSVQGHQKKLEVLQRELTAFKPSIEKVAKLATGLIERNHFDSSNIAEKNAQVG\nQEYEDLLRLAKERESRLGECKKLFEYLRETEELHEWVGDQMAVTASEDYGEDVEHVEQLI\nLAFESFVSNLNANEARVEACLERGDRLIQENNPYRSSIKSKRDETKQLWEELKDLVHARQ\nDALAGAKQVHVYDRVADETIQLINEKDASLISEDYGQDLESIQALGRKHQVFESELVGIQ\nGQVDSVLAEAAKLGEIYPDAKEHIEVKRDETVEAWTDLKEKTAARKNKLSQAEQLQSYFD\nEYRDLIAWINEMLAKITAPELANSVAGAELLLASTKDHDTEIRTRDETFAKFAANGQQLI\nKEKHFLAHEVEDKIKVLQARHELLKHTLNKRREIYELNLDTQLFLKDAEILEQWISSREP\nQLKDTKLGDSIPQVEDLLRRHEDFEKTVAAQEEKFQAIKRITMLEQLFRHQLEQEKISKL\nQEKERLEKERLEQLKQRELQRLADERRRAEKQHEHRQNAASQEKTPIFSSPMVTPAQTSG\nPQSPALSQVQLRPPFGDDNEHLALQKSSSSGMFGDRLRRGSADANVKRAESMKVQPKQAK\nRTPSFTTRRRAQSFRKNQKGEGFDLPPVEIQGSLERKHGLQSGGKKAPVRSWKQFHTVLC\nGQLVCFFKDENDFLQQKTATAPVNILGAKCERADDYTKKKYVFRLKLPDGSEFLFEAPSL\nDILNDWVRKISFHASLPPNMQLLSYDESMKQQSSSSPDIKVTSSVESPVSSRNSSPDSQR\nRTSGAQVLDGTATPQMAFLQRQMQQQQQQQQSQPSSPTGGFDQKPPIPPRGAAPVASHRQ\nSQENLVVMRNRQSSNDLQQSATLPAGLTGVQQNGNGKDDNALLTRNSEARQSGWGNTRFE\nSNRPVSLQPDSISFSRVSAESSSESEAQSISSVSGVKGSKGTKEERRSGMFRIFGRKGDK\nEKEKDKDKRRSSQVPPQ", "mw": 86.594, "total_mw": 173.188, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Fragment of Beta[H] containing segments 25 to 31." } ], "buffer": { "name": "10 mM NaH2PO4, 130 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Beta-Heavy-Spectrin fragment containing segments 25 to 31", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "PSU", "address": "Pennsylvania, USA", "full_name": "Pennsylvania State University (PSU)", "webpage": "https://www.psu.edu/" } ], "contributor_name": "Chelsea", "contributor_surname": "Sarring", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2024-05-23", "storage_temperature": 0.0, "cell_temperature": 4.0, "exposure_time": 600.0, "number_of_frames": 6, "wavelength": 0.154, "sample_detector_distance": 0.5, "concentration_min": null, "concentration_max": 0.55, "sample_volume": null, "flow_rate": null, "s_min": 0.034, "s_max": 6.11, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASD2D2_fit1_model1_img.png", "software": "CORAL", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "3.2.1", "model_data": "https://www.sasbdb.org/media/pdb_file/SASD2D2_fit1_model1.pdb", "model_mw": 166.4, "bead_radius": 1.9, "log": "https://www.sasbdb.org/media/log_files/bends2_BOWTwAd.log", "symmetry": null, "comment": null, "user": 1376 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASD2D2_fit1_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.427, "p_value": 0.0003, "fit_residual_plot": "SASD2D2_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASD2D2_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/BH25to31-4-4-p55mgpml-rep12avg_subtr_bends2_CUFQq3B.log", "software_version": "3.2.1", "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": "The entry contains a dataset of the same sample at a concentration of 1.7 mg/ml, which is available for download as an additional file.", "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 168.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 2.88, "pddf_i0_error": null, "guinier_i0": 2.47251, "guinier_i0_error": null, "pddf_rg": 10.29, "pddf_rg_error": null, "guinier_rg": 7.772, "guinier_rg_error": 0.512, "pddf_dmax": 35.8, "pddf_dmax_error": null, "porod_volume": 453.04, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 7, "guinier_point_last": 17, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASD2D2_datloglog_img.png", "symmetry": null, "last_modified": "2026-08-10T13:59:40.584111+02:00", "bragg_peak": [] }