{ "code": "SASDWD9", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models", "publication": { "title": "KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models", "author_list": "Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T", "journal": "Journal of Molecular Biology", "doi": "10.1016/j.jmb.2025.169103", "pmid": null, "published_date": "2025 Mar" }, "status": "released", "submitted_date": "2025-03-02", "released_date": "2025-03-28" }, "pddf_data": null, "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWD9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWD9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWD9_kratky_img.png", "pddf_plot": null, "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWD9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWD9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Proliferating cell nuclear antigen", "short_name": "PCNA", "sequence": "GPHMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY\r\nRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD\r\nLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI\r\nKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK\r\nIADMGHLKYYLAPKIEDEEGS", "organism": "Homo sapiens", "uniprot_code": "P12004", "uniprot_range_first": 1, "uniprot_range_last": 261, "oligomerization": "trimer", "molecular_type": "protein", "uniprot_sequence": "MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTY\nRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMD\nLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNI\nKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYK\nIADMGHLKYYLAPKIEDEEGS", "mw": 29.06, "total_mw": 87.18, "number_molecules": 3, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "The protein construct used for SAXS contains an additional three non-native amino acids at the N-terminus (GPH)." }, { "long_name": "PCNA-associated factor", "short_name": "p15", "sequence": "VRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK\r\nWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE", "organism": "Homo sapiens", "uniprot_code": "Q15004", "uniprot_range_first": 2, "uniprot_range_last": 111, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPK\nWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE", "mw": 11.855, "total_mw": 11.855, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Disordered protein." } ], "buffer": { "name": "137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS)", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.0, "deuteration": null }, "purity_method": null, "name": "Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:200 µM", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Oeiras, Portugal", "full_name": "Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa", "webpage": "http://www.itqb.unl.pt" } ], "contributor_name": "Tiago", "contributor_surname": "Cordeiro", "orcid": "https://orcid.org/0000-0003-2663-3220" } ], "concentration_method": null, "concentration_unit": null, "date": "2012-04-23", "storage_temperature": 10.0, "cell_temperature": 25.0, "exposure_time": null, "number_of_frames": null, "wavelength": 0.124, "sample_detector_distance": 3.0, "concentration_min": 1.3, "concentration_max": 20.0, "sample_volume": null, "flow_rate": null, "s_min": 0.508, "s_max": 5.0, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": null, "pddf_software_version": null, "i0_calibration_standard": null, "description": "The Rg and the MW are not properly estimated as the profile has been truncated to avoid interparticla interactions. This is a profile from a titration series.", "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 114.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 130.0, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 73.3145, "guinier_i0_error": null, "pddf_rg": null, "pddf_rg_error": null, "guinier_rg": 3.65, "guinier_rg_error": 0.102, "pddf_dmax": 11.0, "pddf_dmax_error": null, "porod_volume": 208.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 10, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDWD9_datloglog_img.png", "symmetry": null, "last_modified": "2025-03-28T13:54:34.579578+01:00", "bragg_peak": [] }