{
"code": "SASDDE3",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/nm",
"project": {
"title": "Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit.",
"publication": {
"title": "Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit.",
"author_list": "Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H",
"journal": "Acta Crystallogr F Struct Biol Commun",
"doi": "10.1107/S2053230X17017381",
"pmid": "29372904",
"published_date": "2018 Jan 1"
},
"status": "released",
"submitted_date": "2018-01-29",
"released_date": "2018-02-06"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDE3.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDE3.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDE3_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDE3_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDE3_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDE3_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDE3.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Pilatus 2M",
"resolution": null
},
"name": "PETRA III",
"city": "DESY; Hamburg",
"country": "Germany",
"beamline_name": "EMBL P12",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Human respiratory syncytial virus M2-1",
"short_name": "HRSV M2-1",
"sequence": "ALGVVGVLESYIGSINNITKQSACVAMSKLLTELNSDDIKKLRDNEEL\r\nNSPKIRVYNTVISYIESNRKNNKQTIHLLKRLPADVLKKTIKNTLDIHKSITINNPKELT\r\nVSDTNDHAKNNDTT",
"organism": "Human orthopneumovirus",
"uniprot_code": "Q4KRW3",
"uniprot_range_first": 73,
"uniprot_range_last": 194,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSRRNPCKFEIRGHCLNGKRCHFSHNYFEWPPHALLVRQNFMLNRILKSMDKSIDTLSEI\nSGAAELDRTEEYALGVVGVLESYIGSINNITKQSACVAMSKLLTELNSDDIKKLRDNEEL\nNSPKIRVYNTVISYIESNRKNNKQTIHLLKRLPADVLKKTIKNTLDIHKSITINNPKELT\nVSDTNDHAKNNDTT",
"mw": 13.658,
"total_mw": 13.658,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "20 mM Tris–HCl, 300 mM NaCl,",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.0,
"deuteration": null
},
"purity_method": null,
"name": "Human respiratory syncytial virus (HRSV) M2–1 RNA-binding core domain",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Hamburg, Germany",
"full_name": "University of Hamburg",
"webpage": "https://www.uni-hamburg.de/"
}
],
"contributor_name": "Yasser",
"contributor_surname": "Almeida",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2016-12-13",
"storage_temperature": 10.0,
"cell_temperature": 10.0,
"exposure_time": 0.045,
"number_of_frames": 20,
"wavelength": 0.124,
"sample_detector_distance": 3.0,
"concentration_min": 4.5,
"concentration_max": 9.0,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.019,
"s_max": 5.007,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDE3_fit1_model1_img.png",
"software": "DAMMIF",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDDE3_fit1_model1.pdb",
"model_mw": 16.761,
"bead_radius": 1.75,
"log": null,
"symmetry": null,
"comment": null,
"user": 282
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDE3_fit1_fit_img.png",
"software": "DAMMIF",
"chi_square_value": 1.232,
"p_value": 0.039236,
"fit_residual_plot": "SASDDE3_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDE3_fit1.fir",
"fit_log": null,
"software_version": null,
"description": null
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of Human respiratory syncytial virus (HRSV) M 2–1 RNA-binding core domain in 20 mM Tris-HCl, 300 mM NaCl, pH 7, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 4.5 and 9 mg/ml were measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 15.0,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 15.0,
"porod_mw_error": null,
"pddf_i0": 3865.0,
"pddf_i0_error": 5.1,
"guinier_i0": 3828.08,
"guinier_i0_error": 3.24,
"pddf_rg": 2.14,
"pddf_rg_error": 0.005,
"guinier_rg": 2.03,
"guinier_rg_error": null,
"pddf_dmax": 7.9,
"pddf_dmax_error": null,
"porod_volume": 2.64,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 63,
"guinier_point_last": 228,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDDE3_datloglog_img.png",
"symmetry": null,
"last_modified": "2023-06-21T07:56:39.492710+02:00",
"bragg_peak": []
}