data_SASDFF2_MAIN # _sas_result.id 1294 _sas_result.SASBDB_code SASDFF2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 20.8000000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 10.530 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 8 _sas_result.Guinier_point_max 17 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 245-249. ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249)" _sas_scan.detc_id 15 _sas_scan.id 1294 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1255 _sas_scan.result_id 1294 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1255 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 245-249)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1255 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 2.217600E+01 1.497849E+01 1294 2 5.968380E-03 1.750380E+01 8.827546E+00 1294 3 6.388220E-03 1.812420E+01 6.408974E+00 1294 4 6.808050E-03 2.294140E+01 4.909324E+00 1294 5 7.227880E-03 2.122560E+01 3.593338E+00 1294 6 7.647710E-03 1.901260E+01 3.168328E+00 1294 7 8.067540E-03 1.918020E+01 2.676896E+00 1294 8 8.487370E-03 1.406304E+01 2.438060E+00 1294 9 8.907200E-03 1.498078E+01 2.055222E+00 1294 10 9.327030E-03 1.508950E+01 1.920116E+00 1294 11 9.746860E-03 1.539410E+01 1.904072E+00 1294 12 1.016670E-02 1.525940E+01 1.773058E+00 1294 13 1.058650E-02 1.291394E+01 1.488312E+00 1294 14 1.100630E-02 1.448540E+01 1.425020E+00 1294 15 1.142620E-02 1.285856E+01 1.327690E+00 1294 16 1.184600E-02 1.166770E+01 1.128452E+00 1294 17 1.226580E-02 1.184524E+01 1.152272E+00 1294 18 1.268570E-02 1.068694E+01 1.063788E+00 1294 19 1.310550E-02 1.188752E+01 1.085804E+00 1294 20 1.352530E-02 9.650240E+00 1.003399E+00 1294 21 1.394520E-02 9.364900E+00 9.214210E-01 1294 22 1.436500E-02 8.848080E+00 8.913478E-01 1294 23 1.478480E-02 8.179980E+00 8.466800E-01 1294 24 1.520460E-02 7.598000E+00 8.466806E-01 1294 25 1.562450E-02 7.301580E+00 7.504264E-01 1294 26 1.604430E-02 7.217540E+00 7.479844E-01 1294 27 1.646410E-02 7.360860E+00 6.666152E-01 1294 28 1.688400E-02 6.233820E+00 6.323850E-01 1294 29 1.730380E-02 6.742140E+00 6.490684E-01 1294 30 1.772360E-02 6.079160E+00 5.920428E-01 1294 31 1.814350E-02 5.983600E+00 6.018126E-01 1294 32 1.856330E-02 5.521980E+00 5.610058E-01 1294 33 1.898310E-02 5.604360E+00 5.474268E-01 1294 34 1.940290E-02 5.442200E+00 5.421590E-01 1294 35 1.982280E-02 5.413420E+00 5.336200E-01 1294 36 2.024260E-02 4.997260E+00 5.339706E-01 1294 37 2.066240E-02 4.637320E+00 5.071176E-01 1294 38 2.108230E-02 4.174560E+00 4.778432E-01 1294 39 2.150210E-02 3.930500E+00 4.919188E-01 1294 40 2.192190E-02 3.914500E+00 4.605860E-01 1294 41 2.234180E-02 3.525840E+00 4.789546E-01 1294 42 2.276160E-02 3.413180E+00 4.342304E-01 1294 43 2.318140E-02 3.561620E+00 4.367244E-01 1294 44 2.360130E-02 3.290220E+00 4.382530E-01 1294 45 2.402110E-02 3.125000E+00 4.247626E-01 1294 46 2.444090E-02 3.210360E+00 4.229882E-01 1294 47 2.486070E-02 2.653280E+00 4.045136E-01 1294 48 2.528060E-02 2.559120E+00 4.172576E-01 1294 49 2.570040E-02 2.660320E+00 3.983272E-01 1294 50 2.612020E-02 2.957000E+00 3.943648E-01 1294 51 2.654010E-02 2.189420E+00 3.791960E-01 1294 52 2.695990E-02 2.225660E+00 3.667424E-01 1294 53 2.737970E-02 2.498660E+00 3.927326E-01 1294 54 2.779960E-02 2.117240E+00 3.710488E-01 1294 55 2.821940E-02 2.160980E+00 3.718240E-01 1294 56 2.863920E-02 1.933580E+00 3.701122E-01 1294 57 2.905900E-02 2.121820E+00 3.673884E-01 1294 58 2.947890E-02 2.123500E+00 3.737840E-01 1294 59 2.989870E-02 1.998860E+00 3.611896E-01 1294 60 3.031850E-02 1.521960E+00 3.661986E-01 1294 61 3.073840E-02 2.016240E+00 3.677228E-01 1294 62 3.115820E-02 1.456480E+00 3.619208E-01 1294 63 3.157800E-02 1.459660E+00 3.512522E-01 1294 64 3.199790E-02 1.535980E+00 3.507130E-01 1294 65 3.241770E-02 1.615000E+00 3.600854E-01 1294 66 3.283750E-02 1.411080E+00 3.511950E-01 1294 67 3.325730E-02 1.500540E+00 3.608666E-01 1294 68 3.367720E-02 1.589560E+00 3.561368E-01 1294 69 3.409700E-02 1.500880E+00 3.465782E-01 1294 70 3.451680E-02 9.784798E-01 3.473464E-01 1294 71 3.493670E-02 1.274680E+00 3.355440E-01 1294 72 3.535650E-02 1.259840E+00 3.378188E-01 1294 73 3.577630E-02 1.241020E+00 3.336398E-01 1294 74 3.619620E-02 1.515240E+00 3.444228E-01 1294 75 3.661600E-02 1.303800E+00 3.407966E-01 1294 76 3.703580E-02 1.205660E+00 3.260616E-01 1294 77 3.745560E-02 1.076080E+00 3.286056E-01 1294 78 3.787550E-02 1.121980E+00 3.119038E-01 1294 79 3.829530E-02 9.731400E-01 3.151520E-01 1294 80 3.871510E-02 1.035740E+00 3.097366E-01 1294 81 3.913500E-02 9.802798E-01 2.993906E-01 1294 82 3.955480E-02 9.849800E-01 3.132260E-01 1294 83 3.997460E-02 8.954802E-01 3.088376E-01 1294 84 4.039450E-02 1.021120E+00 3.055836E-01 1294 85 4.081430E-02 8.874002E-01 3.012334E-01 1294 86 4.123410E-02 9.374202E-01 2.977942E-01 1294 87 4.165390E-02 9.429400E-01 2.935762E-01 1294 88 4.207380E-02 7.767804E-01 2.885186E-01 1294 89 4.249360E-02 9.433596E-01 2.892400E-01 1294 90 4.291340E-02 6.992000E-01 2.840134E-01 1294 91 4.333330E-02 7.107800E-01 2.863636E-01 1294 92 4.375310E-02 9.000000E-01 2.762148E-01 1294 93 4.417290E-02 8.714606E-01 2.706350E-01 1294 94 4.459280E-02 7.299400E-01 2.695902E-01 1294 95 4.501260E-02 7.962600E-01 2.678488E-01 1294 96 4.543240E-02 8.522602E-01 2.711676E-01 1294 97 4.585220E-02 4.591804E-01 2.599274E-01 1294 98 4.627210E-02 7.304400E-01 2.650492E-01 1294 99 4.669190E-02 9.024000E-01 2.713570E-01 1294 100 4.711170E-02 6.212400E-01 2.627016E-01 1294 101 4.753160E-02 5.162598E-01 2.604186E-01 1294 102 4.795140E-02 8.163000E-01 2.585150E-01 1294 103 4.837120E-02 7.437200E-01 2.548776E-01 1294 104 4.879110E-02 6.405402E-01 2.624990E-01 1294 105 4.921090E-02 4.490000E-01 2.487356E-01 1294 106 4.963070E-02 5.910601E-01 2.560756E-01 1294 107 5.005050E-02 5.385998E-01 2.591848E-01 1294 108 5.047040E-02 6.277400E-01 2.497866E-01 1294 109 5.089020E-02 7.315798E-01 2.489664E-01 1294 110 5.131000E-02 6.855200E-01 2.474224E-01 1294 111 5.172990E-02 6.339406E-01 2.469408E-01 1294 112 5.214970E-02 5.818594E-01 2.452776E-01 1294 113 5.256950E-02 5.732794E-01 2.472758E-01 1294 114 5.298940E-02 3.921204E-01 2.404718E-01 1294 115 5.340920E-02 6.377402E-01 2.436794E-01 1294 116 5.382900E-02 4.218398E-01 2.338338E-01 1294 117 5.424890E-02 5.801394E-01 2.428456E-01 1294 118 5.466870E-02 7.055800E-01 2.463378E-01 1294 119 5.508850E-02 4.356398E-01 2.434720E-01 1294 120 5.550830E-02 4.162801E-01 2.337314E-01 1294 121 5.592820E-02 6.175804E-01 2.374176E-01 1294 122 5.634800E-02 6.985598E-01 2.361594E-01 1294 123 5.676780E-02 3.966800E-01 2.350560E-01 1294 124 5.718770E-02 3.895192E-01 2.284454E-01 1294 125 5.760750E-02 5.343398E-01 2.301904E-01 1294 126 5.802730E-02 3.698398E-01 2.239348E-01 1294 127 5.844720E-02 5.497194E-01 2.260818E-01 1294 128 5.886700E-02 6.893002E-01 2.292938E-01 1294 129 5.928680E-02 5.093606E-01 2.171878E-01 1294 130 5.970660E-02 3.900198E-01 2.259248E-01 1294 131 6.012650E-02 4.970202E-01 2.231576E-01 1294 132 6.054630E-02 3.541401E-01 2.177566E-01 1294 133 6.096610E-02 3.715598E-01 2.203902E-01 1294 134 6.138600E-02 4.844404E-01 2.181608E-01 1294 135 6.180580E-02 3.218800E-01 2.184606E-01 1294 136 6.222560E-02 2.370200E-01 2.208462E-01 1294 137 6.264550E-02 5.874800E-01 2.195594E-01 1294 138 6.306530E-02 4.446603E-01 2.223998E-01 1294 139 6.348510E-02 3.417000E-01 2.131164E-01 1294 140 6.390490E-02 3.983000E-01 2.205730E-01 1294 141 6.432480E-02 2.757201E-01 2.128984E-01 1294 142 6.474460E-02 4.946400E-01 2.151792E-01 1294 143 6.516440E-02 3.994804E-01 2.145450E-01 1294 144 6.558430E-02 4.656802E-01 2.104154E-01 1294 145 6.600410E-02 4.399206E-01 2.084820E-01 1294 146 6.642390E-02 4.292400E-01 2.082528E-01 1294 147 6.684380E-02 3.727202E-01 2.112386E-01 1294 148 6.726360E-02 3.383200E-01 2.107962E-01 1294 149 6.768340E-02 3.662400E-01 2.099528E-01 1294 150 6.810320E-02 4.009400E-01 2.086894E-01 1294 151 6.852310E-02 5.432202E-01 2.098884E-01 1294 152 6.894290E-02 3.171202E-01 2.065076E-01 1294 153 6.936270E-02 3.381200E-01 2.073552E-01 1294 154 6.978260E-02 1.927200E-01 2.041982E-01 1294 155 7.020240E-02 3.283995E-01 2.046018E-01 1294 156 7.062220E-02 4.501400E-01 2.015632E-01 1294 157 7.104210E-02 4.067802E-01 2.029362E-01 1294 158 7.146190E-02 2.888996E-01 1.993596E-01 1294 159 7.188170E-02 4.395196E-01 2.021312E-01 1294 160 7.230160E-02 4.582600E-01 2.014590E-01 1294 161 7.272140E-02 3.755204E-01 1.991472E-01 1294 162 7.314120E-02 4.313796E-01 2.003904E-01 1294 163 7.356100E-02 3.954804E-01 1.941472E-01 1294 164 7.398090E-02 4.025404E-01 1.991686E-01 1294 165 7.440070E-02 2.319796E-01 1.924036E-01 1294 166 7.482050E-02 1.806004E-01 1.992472E-01 1294 167 7.524040E-02 2.591994E-02 1.970648E-01 1294 168 7.566020E-02 2.771804E-01 1.927810E-01 1294 169 7.608000E-02 4.832600E-01 1.962928E-01 1294 170 7.649980E-02 2.721798E-01 1.940606E-01 1294 171 7.691970E-02 2.296796E-01 1.925292E-01 1294 172 7.733950E-02 3.787801E-01 1.930908E-01 1294 173 7.775930E-02 5.219798E-01 1.947606E-01 1294 174 7.817920E-02 4.232400E-01 1.951814E-01 1294 175 7.859900E-02 3.513002E-01 1.946164E-01 1294 176 7.901880E-02 4.486198E-01 1.983094E-01 1294 177 7.943870E-02 2.874600E-01 1.917378E-01 1294 178 7.985850E-02 2.735204E-01 1.916170E-01 1294 179 8.027830E-02 3.782803E-01 1.922846E-01 1294 180 8.069810E-02 3.413998E-01 1.924288E-01 1294 181 8.111800E-02 7.373998E-02 1.943902E-01 1294 182 8.153780E-02 2.597805E-01 1.871518E-01 1294 183 8.195760E-02 3.558797E-01 1.938094E-01 1294 184 8.237750E-02 3.144402E-01 1.889166E-01 1294 185 8.279730E-02 1.953598E-01 1.929956E-01 1294 186 8.321710E-02 1.575195E-01 1.894684E-01 1294 187 8.363700E-02 2.316199E-01 1.911006E-01 1294 188 8.405680E-02 2.925400E-01 1.984288E-01 1294 189 8.447660E-02 2.657402E-01 2.024352E-01 1294 190 8.489650E-02 1.565200E-01 1.999566E-01 1294 191 8.531630E-02 2.882403E-01 2.031914E-01 1294 192 8.573610E-02 3.476602E-01 2.003296E-01 1294 193 8.615590E-02 2.318798E-01 2.061536E-01 1294 194 8.657580E-02 2.061804E-01 2.024298E-01 1294 195 8.699560E-02 2.461203E-01 2.049158E-01 1294 196 8.741540E-02 1.289395E-01 2.015636E-01 1294 197 8.783530E-02 1.981799E-01 2.053386E-01 1294 198 8.825510E-02 2.289198E-01 2.036730E-01 1294 199 8.867490E-02 1.353000E-01 2.122544E-01 1294 200 8.909480E-02 7.229986E-02 2.041992E-01 1294 201 8.951460E-02 1.993796E-01 2.062316E-01 1294 202 8.993440E-02 3.103401E-01 2.047552E-01 1294 203 9.035420E-02 2.735998E-01 2.062576E-01 1294 204 9.077410E-02 1.691600E-01 2.026670E-01 1294 205 9.119390E-02 3.350002E-01 2.064882E-01 1294 206 9.161370E-02 2.212802E-01 2.034922E-01 1294 207 9.203360E-02 4.339996E-01 2.060224E-01 1294 208 9.245340E-02 3.441808E-01 2.087392E-01 1294 209 9.287320E-02 2.878402E-01 2.084512E-01 1294 210 9.329310E-02 1.438404E-01 2.122634E-01 1294 211 9.371290E-02 2.537202E-01 2.115572E-01 1294 212 9.413270E-02 1.066805E-01 2.082952E-01 1294 213 9.455250E-02 7.034032E-02 2.049148E-01 1294 214 9.497240E-02 1.732798E-01 2.093720E-01 1294 215 9.539220E-02 1.848200E-01 2.055346E-01 1294 216 9.581200E-02 1.313405E-01 2.035118E-01 1294 217 9.623190E-02 2.890800E-01 2.079562E-01 1294 218 9.665170E-02 1.237593E-01 2.042728E-01 1294 219 9.707150E-02 2.474000E-01 2.066954E-01 1294 220 9.749140E-02 2.272403E-01 2.033896E-01 1294 221 9.791120E-02 8.280012E-02 1.970718E-01 1294 222 9.833100E-02 1.509596E-01 1.996376E-01 1294 223 9.875080E-02 1.353198E-01 2.007600E-01 1294 224 9.917070E-02 1.927397E-01 2.015728E-01 1294 225 9.959050E-02 3.743608E-01 2.002180E-01 1294 226 1.000100E-01 2.908006E-01 1.979876E-01 1294 227 1.004300E-01 1.501797E-01 1.983016E-01 1294 228 1.008500E-01 2.014199E-01 1.932082E-01 1294 229 1.012700E-01 1.980603E-01 1.978138E-01 1294 230 1.016900E-01 2.192995E-01 1.951788E-01 1294 231 1.021090E-01 3.595202E-01 1.965938E-01 1294 232 1.025290E-01 -4.620360E-03 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