{
"code": "SASDXF2",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "Insight into the structure and interactions of the M. tuberculosis\n Mce-associated membrane proteins Mam1A-1D",
"publication": {
"title": "Insight into the structure and interactions of the M. tuberculosis\n Mce-associated membrane proteins Mam1A-1D",
"author_list": "Hynönen M, Perumal P, Hynönen N, Doutch J, Ma K, Venkatesan R",
"journal": null,
"doi": "10.1101/2025.03.26.645568",
"pmid": null,
"published_date": "2025 Mar 26"
},
"status": "released",
"submitted_date": "2025-03-26",
"released_date": "2025-04-11"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXF2.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXF2.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXF2_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXF2_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXF2_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXF2_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXF2.sascif",
"experiment": {
"instrument": {
"detector": {
"type": null,
"name": "Eiger 4M",
"resolution": 75.0
},
"name": "Diamond Light Source",
"city": "Didcot",
"country": "UK",
"beamline_name": "B21",
"beam_geometry": null,
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Probable conserved Mce associated membrane protein",
"short_name": "Mam1A delta 106",
"sequence": "MKHHHHHHPMSDYDIPTTENLYFQGAMAPDAGAMSASMQKIIECGTGDFGAQASLYTSMLVEAYQAASVHVQVTDMRAAVERNNNDGSVDVLVALRVKVSNTDSDAHEVGYRLRVRMALDEGRYKIAKLDQVTK",
"organism": "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)",
"uniprot_code": "O07419",
"uniprot_range_first": 107,
"uniprot_range_last": 213,
"oligomerization": "tetramer",
"molecular_type": "protein",
"uniprot_sequence": "MKAADSAESDAGADQTGPQVKAADSAESDAGELGEDACPEQALVERRPSRLRRGWLVGIA\nATLLALAGGLGAAGYFALRSHQESQSIAREDLAAIEAAKDCVAATQAPDAGAMSASMQKI\nIECGTGDFGAQASLYTSMLVEAYQAASVHVQVTDMRAAVERNNNDGSVDVLVALRVKVSN\nTDSDAHEVGYRLRVRMALDEGRYKIAKLDQVTK",
"mw": 14.807,
"total_mw": 59.228,
"number_molecules": 4,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "Mam1A/Rv0175 delta 106 is N-terminally truncated construct which lacks the alpha1-helix. Protein, despite the removal of TM-helix, is not soluble and required C12E9 detergent to be solubilized. Tetrameric form was calculated with SEC-MALS."
}
],
"buffer": {
"name": "50mM Tris, 500mM NaCl",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 8.5,
"deuteration": null
},
"purity_method": null,
"name": "Probable conserved Mce associated membrane protein (Mam1Adelta106) from M.tuberculosis",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": null,
"full_name": "University of Helsinki",
"webpage": null
}
],
"contributor_name": "Mikko",
"contributor_surname": "Hynönen",
"orcid": "https://orcid.org/0000-0003-2431-8892"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2019-12-08",
"storage_temperature": 15.0,
"cell_temperature": 15.0,
"exposure_time": 3.0,
"number_of_frames": 600,
"wavelength": 0.09464,
"sample_detector_distance": 3.7329,
"concentration_min": null,
"concentration_max": 8.8,
"sample_volume": 35.0,
"flow_rate": 0.07,
"s_min": 0.026,
"s_max": 3.4,
"total_exposure_time": null,
"seccolumn": 14
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDXF2_fit1_model1_img.png",
"software": "AlphaFold",
"pdb_link": [],
"model_title": "1A_107_Main_Model_Tetra_SASDXF2",
"type_of_model": "dummy",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDXF2_fit1_model1.pdb",
"model_mw": null,
"bead_radius": 1.9,
"log": null,
"symmetry": null,
"comment": null,
"user": 1328
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDXF2_fit1_fixed_fit_img.png",
"software": "FoXS",
"chi_square_value": 31.206491,
"p_value": 0.0,
"fit_residual_plot": "SASDXF2_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDXF2_fit1.fit",
"fit_log": null,
"software_version": null,
"description": ""
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": "The truncated version of the data is provided as an additional file.",
"experiment_description": null,
"tags": [],
"intensity_unit": "1/cm",
"experimental_mw": 39.4,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 56.0,
"porod_mw_error": null,
"pddf_i0": 0.03975,
"pddf_i0_error": null,
"guinier_i0": 0.0401827,
"guinier_i0_error": null,
"pddf_rg": 3.22,
"pddf_rg_error": null,
"guinier_rg": 3.22,
"guinier_rg_error": 0.086,
"pddf_dmax": 9.0,
"pddf_dmax_error": null,
"porod_volume": 177.0,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 41,
"guinier_point_last": 275,
"pddf_point_first": 29,
"pddf_point_last": 841,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDXF2_datloglog_img.png",
"symmetry": null,
"last_modified": "2026-05-21T15:22:41.639400+02:00",
"bragg_peak": []
}