{ "code": "SASDDF6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Internal nanosecond dynamics in the intrinsically disordered myelin basic protein.", "publication": { "title": "Internal nanosecond dynamics in the intrinsically disordered myelin basic protein.", "author_list": "Stadler AM, Stingaciu L, Radulescu A, Holderer O, Monkenbusch M, Biehl R, Richter D", "journal": "J Am Chem Soc", "doi": "10.1021/ja502343b", "pmid": "24758710", "published_date": "2014 May 14" }, "status": "released", "submitted_date": "2018-05-08", "released_date": "2018-05-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDF6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDF6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDF6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDF6.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Myelin basic protein", "short_name": null, "sequence": ">sp|P02687|1-169\r\nAAQKRPSQRSKYLASASTMDHARHGFLPRHRDTGILDSLGRFFGSDRGAPKRGSGKDGHH\r\nAARTTHYGSLPQKAQGHRPQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQ\r\nKPGFGYGGRASDYKSAHKGLKGHDAQGTLSKIFKLGGRDSRSGSPMARR", "organism": "Bos taurus", "uniprot_code": "P02687", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "AAQKRPSQRSKYLASASTMDHARHGFLPRHRDTGILDSLGRFFGSDRGAPKRGSGKDGHH\nAARTTHYGSLPQKAQGHRPQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQ\nKPGFGYGGRASDYKSAHKGLKGHDAQGTLSKIFKLGGRDSRSGSPMARR", "mw": 18.323, "total_mw": 18.323, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM NaH2PO4/ Na2HPO4, 99.9% D2O", "concentration_unit": null, "comment": "deuterium oxide", "additive": null, "concentration": null, "pka": null, "ph": 4.8, "deuteration": null }, "purity_method": null, "name": "Myelin basic protein", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Germany", "full_name": "Forschungszentrum Jülich", "webpage": "https://www.fz-juelich.de" } ], "contributor_name": "Andreas", "contributor_surname": "Stadler", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2013-02-21", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 2.0, "number_of_frames": 10, "wavelength": 0.099, "sample_detector_distance": null, "concentration_min": null, "concentration_max": 4.5, "sample_volume": null, "flow_rate": null, "s_min": 0.137, "s_max": 4.525, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model1_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model1.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model2_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model2.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model3_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model3.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model4_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model4.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model5_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model5.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF6_fit1_model6_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF6_fit1_model6.pdb", "model_mw": 18.59, "bead_radius": 1.9, "log": null, "symmetry": null, "comment": null, "user": 317 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDF6_fit1_fixed_fit_img.png", "software": "EOM/GAJOE", "chi_square_value": 1.183582, "p_value": 0.000947, "fit_residual_plot": "fit4columns1422_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDF6_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_OIne5hA.log", "software_version": "2.5", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of myelin basic protein in 20 mM NaH2PO4/Na2HPO4, 99.9% v/v D2O, pH 4.8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 10 successive 2 second frames were collected at 10°C from a sample at 4.5 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [ "idp" ], "intensity_unit": "arbitrary", "experimental_mw": 20.49, "experimental_mw_error": null, "guinier_i0_mw": 20.49, "guinier_i0_mw_error": 0.01, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 20.72, "pddf_i0_error": null, "guinier_i0": 20.49, "guinier_i0_error": 0.023, "pddf_rg": 3.461, "pddf_rg_error": null, "guinier_rg": 3.3, "guinier_rg_error": 0.01, "pddf_dmax": 11.06, "pddf_dmax_error": null, "porod_volume": null, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 8, "guinier_point_last": 60, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDF6_datloglog_img.png", "symmetry": null, "last_modified": "2020-08-28T15:28:05.022045+02:00", "bragg_peak": [] }