{ "code": "SASDWF7", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "SAXS analysis of EccA5 N-terminal domain and EspG5 of ESX-5 secretion pathway of Mycobacterium tuberculosis", "publication": null, "status": "released", "submitted_date": "2025-02-12", "released_date": "2026-02-12" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDWF7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDWF7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDWF7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDWF7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDWF7.sascif", "experiment": { "instrument": { "detector": { "type": "Hybrid Photon Counting (HPC)", "name": "Mythen2 R 1K", "resolution": null }, "name": "CSIR-Central Drug Research Institute", "city": "Lucknow", "country": "India", "beamline_name": "Anton Paar SAXSpace", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "ESX-5 secretion-associated protein EspG5", "short_name": "EspG5", "sequence": "MDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV", "organism": "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)", "uniprot_code": "O53943", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MDQQSTRTDITVNVDGFWMLQALLDIRHVAPELRCRPYVSTDSNDWLNEHPGMAVMREQG\nIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVL\nARRGQHWVSAVRVGNDITVDDVTVSDSASIAALVMDGLESIHHADPAAINAVNVPMEEML\nEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSAS\nVLSLKDGSGGRIALYQQARTAGSGEAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHSRV", "mw": 32.4, "total_mw": 32.4, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" }, { "long_name": "ESX-5 secretion system protein EccA5 N-terminal domain", "short_name": "EccA5NT", "sequence": "GSHMTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARLLAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAAVLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPELKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAVELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEAR", "organism": "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)", "uniprot_code": "P9WPI1", "uniprot_range_first": 1, "uniprot_range_last": 278, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MTRPQAAAEDARNAMVAGLLASGISVNGLQPSHNPQVAAQMFTTATRLDPKMCDAWLARL\nLAGDQSIEVLAGAWAAVRTFGWETRRLGVTDLQFRPEVSDGLFLRLAITSVDSLACAYAA\nVLAEAKRYQEAAELLDATDPRHPFDAELVSYVRGVLYFRTKRWPDVLAQFPEATQWRHPE\nLKAAGAAMATTALASLGVFEEAFRRAQEAIEGDRVPGAANIALYTQGMCLRHVGREEEAV\nELLRRVYSRDAKFTPAREALDNPNFRLILTDPETIEARTDPWDPDSAPTRAQTEAARHAE\nMAAKYLAEGDAELNAMLGMEQAKKEIKLIKSTTKVNLARAKMGLPVPVTSRHTLLLGPPG\nTGKTSVARAFTKQLCGLTVLRKPLVVETSRTKLLGRYMADAEKNTEEMLEGALGGAVFFD\nEMHTLHEKGYSQGDPYGNAIINTLLLYMENHRDELVVFGAGYAKAMEKMLEVNQGLRRRF\nSTVIEFFSYTPQELIALTQLMGRENEDVITEEESQVLLPSYTKFYMEQSYSEDGDLIRGI\nDLLGNAGFVRNVVEKARDHRSFRLDDEDLDAVLASDLTEFSEDQLRRFKELTREDLAEGL\nRAAVAEKKTK", "mw": 30.9, "total_mw": 30.9, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "" } ], "buffer": { "name": "50mM Hepes 200mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "EccA5-N terminal domain and EspG5 complex of ESX-5 secretion pathway of Mycobacterium tuberculosis", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "India", "full_name": "CSIR - Central Drug Research Institute", "webpage": "https://cdri.res.in/" } ], "contributor_name": "Rajlakshmi", "contributor_surname": "K", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2025-01-28", "storage_temperature": 4.0, "cell_temperature": 10.0, "exposure_time": 900.0, "number_of_frames": 4, "wavelength": 0.154, "sample_detector_distance": 0.317, "concentration_min": null, "concentration_max": 4.0, "sample_volume": null, "flow_rate": null, "s_min": 0.094, "s_max": 6.001, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDWF7_fit1_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "DAMFILT 5.0 (r13837)", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDWF7_fit1_model1.pdb", "model_mw": 55.623, "bead_radius": 2.75, "log": "https://www.sasbdb.org/media/log_files/G5NT_4MG-damsel_tVpca9h.log", "symmetry": null, "comment": null, "user": 1172 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDWF7_fit1_fixed_fit_img.png", "software": "DAMMIF", "chi_square_value": 0.026, "p_value": 0.0, "fit_residual_plot": "SASDWF7_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDWF7_fit1.fir", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/G5NT_4MG_6HpEiT6.log", "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 53.15, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 55.0, "porod_mw_error": null, "pddf_i0": 66240.0, "pddf_i0_error": null, "guinier_i0": 66692.4, "guinier_i0_error": null, "pddf_rg": 3.362, "pddf_rg_error": null, "guinier_rg": 3.319, "guinier_rg_error": 0.398, "pddf_dmax": 10.74, "pddf_dmax_error": null, "porod_volume": 84.25, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 5, "guinier_point_last": 48, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDWF7_datloglog_img.png", "symmetry": null, "last_modified": "2026-02-22T17:39:35.038740+01:00", "bragg_peak": [] }