{ "code": "SASDDF9", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.", "publication": { "title": "OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.", "author_list": "Sluchanko NN, Slonimskiy YB, Shirshin EA, Moldenhauer M, Friedrich T, Maksimov EG", "journal": "Nat Commun", "doi": "10.1038/s41467-018-06195-0", "pmid": "30250028", "published_date": "2018 Sep 24" }, "status": "released", "submitted_date": "2018-07-04", "released_date": "2018-08-07" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDF9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDF9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDF9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDF9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDF9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Orange carotenoid-binding protein", "short_name": "OCP", "sequence": ">dNTE\r\nMRGSHHHHHHTDPATVPATIARFSQLNAEDQLALIWFAYLEMGKTLTIAAPGAASMQLAENALKEIQAMGPLQQTQAMCDLANRADTPLCRTYASWSPNIKLGFWYRLGELMEQG FVAPIPAGYQLSANANAVLATIQGLESGQQITVLRNAVVDMGFTAGKDGKRIAEPVVPPQ DTASRTKVSIEGVTNATVLNYMDNLNANDFDTLIELFTSDGALQPPFQRPIVGKENVLRF FREECQNLKLIPERGVTEPAEDGFTQIKVTGKVQTPWFGGNVGMNIAWRFLLNPEGKIFF VAIDLLASPKELLNFAR", "organism": "Synechocystis sp. PCC 6803", "uniprot_code": "P74102", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MPFTIDSARGIFPNTLAADVVPATIARFSQLNAEDQLALIWFAYLEMGKTLTIAAPGAAS\nMQLAENALKEIQAMGPLQQTQAMCDLANRADTPLCRTYASWSPNIKLGFWYRLGELMEQG\nFVAPIPAGYQLSANANAVLATIQGLESGQQITVLRNAVVDMGFTAGKDGKRIAEPVVPPQ\nDTASRTKVSIEGVTNATVLNYMDNLNANDFDTLIELFTSDGALQPPFQRPIVGKENVLRF\nFREECQNLKLIPERGVTEPAEDGFTQIKVTGKVQTPWFGGNVGMNIAWRFLLNPEGKIFF\nVAIDLLASPKELLNFAR", "mw": 34.28, "total_mw": 34.28, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris, 150 mM NaCl, 3% glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.6, "deuteration": null }, "purity_method": null, "name": "Synechocystis orange carotenoid-binding protein devoid of the NTE (OCP-ΔNTE, orange form)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Moscow, Russia", "full_name": "A.N. Bach Institute of Biochemistry", "webpage": "http://www.fbras.ru/en/" } ], "contributor_name": "Nikolai", "contributor_surname": "Sluchanko", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2017-09-01", "storage_temperature": 20.0, "cell_temperature": 10.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.124, "sample_detector_distance": null, "concentration_min": 0.4, "concentration_max": 3.11, "sample_volume": null, "flow_rate": null, "s_min": 0.127, "s_max": 4.162, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDF9_fit1_model1_img.png", "software": "CORAL", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDF9_fit1_model1.pdb", "model_mw": 34.08, "bead_radius": 1.9, "log": "https://www.sasbdb.org/media/log_files/cor1.log", "symmetry": null, "comment": null, "user": 267 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDF9_fit1_fit_img.png", "software": "CORAL", "chi_square_value": 1.153, "p_value": 0.021977, "fit_residual_plot": "SASDDF9_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDF9_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/cor1.log", "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Synechocystis OCP-ΔNTE (orange form) in 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 3 % v/v glycerol, pH 7.6 were collected at the EMBL-P12 bioSAXS beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 1.75 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data scaled for protein concentration.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 35.5, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 35.5, "porod_mw_error": null, "pddf_i0": 0.03321, "pddf_i0_error": 0.0001, "guinier_i0": 0.033, "guinier_i0_error": 0.00016, "pddf_rg": 2.268, "pddf_rg_error": 0.007, "guinier_rg": 2.24, "guinier_rg_error": 0.05, "pddf_dmax": 7.4, "pddf_dmax_error": null, "porod_volume": 56.8, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 165, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDF9_datloglog_img.png", "symmetry": null, "last_modified": "2023-11-13T09:35:03.156077+01:00", "bragg_peak": [] }