data_SASDFG2_MAIN # _sas_result.id 1295 _sas_result.SASBDB_code SASDFG2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 30.2900000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 11.400 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 2 _sas_result.Guinier_point_max 13 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 250-254 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254)" _sas_scan.detc_id 15 _sas_scan.id 1295 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1256 _sas_scan.result_id 1295 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1256 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 250-254)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1256 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 1.301600E+01 1.158437E+01 1295 2 5.968380E-03 3.001740E+01 9.361884E+00 1295 3 6.388220E-03 2.313020E+01 5.820370E+00 1295 4 6.808050E-03 2.662640E+01 4.438630E+00 1295 5 7.227880E-03 2.700380E+01 3.949390E+00 1295 6 7.647710E-03 2.373920E+01 3.091980E+00 1295 7 8.067540E-03 1.952660E+01 2.447932E+00 1295 8 8.487370E-03 2.201440E+01 2.434144E+00 1295 9 8.907200E-03 2.021092E+01 2.174304E+00 1295 10 9.327030E-03 2.078326E+01 1.839490E+00 1295 11 9.746860E-03 1.988944E+01 1.843754E+00 1295 12 1.016670E-02 2.023892E+01 1.704806E+00 1295 13 1.058650E-02 1.833226E+01 1.489606E+00 1295 14 1.100630E-02 1.640282E+01 1.433816E+00 1295 15 1.142620E-02 1.667668E+01 1.364314E+00 1295 16 1.184600E-02 1.642570E+01 1.258696E+00 1295 17 1.226580E-02 1.505676E+01 1.188770E+00 1295 18 1.268570E-02 1.500044E+01 1.157666E+00 1295 19 1.310550E-02 1.453286E+01 1.162096E+00 1295 20 1.352530E-02 1.273162E+01 9.526762E-01 1295 21 1.394520E-02 1.242070E+01 9.747704E-01 1295 22 1.436500E-02 1.254086E+01 9.510916E-01 1295 23 1.478480E-02 1.120366E+01 8.982864E-01 1295 24 1.520460E-02 1.127202E+01 9.109880E-01 1295 25 1.562450E-02 1.074146E+01 7.837218E-01 1295 26 1.604430E-02 1.048468E+01 7.367184E-01 1295 27 1.646410E-02 9.590920E+00 7.162534E-01 1295 28 1.688400E-02 9.183100E+00 6.449654E-01 1295 29 1.730380E-02 8.731820E+00 6.958044E-01 1295 30 1.772360E-02 8.683700E+00 6.448154E-01 1295 31 1.814350E-02 8.452960E+00 6.251834E-01 1295 32 1.856330E-02 8.168240E+00 5.941168E-01 1295 33 1.898310E-02 7.977640E+00 5.682214E-01 1295 34 1.940290E-02 7.200120E+00 5.518166E-01 1295 35 1.982280E-02 7.377560E+00 5.440706E-01 1295 36 2.024260E-02 6.115800E+00 5.326280E-01 1295 37 2.066240E-02 6.218800E+00 5.174364E-01 1295 38 2.108230E-02 5.899200E+00 5.165834E-01 1295 39 2.150210E-02 5.652500E+00 5.019770E-01 1295 40 2.192190E-02 5.356240E+00 4.723628E-01 1295 41 2.234180E-02 5.264700E+00 4.813960E-01 1295 42 2.276160E-02 4.794360E+00 4.572732E-01 1295 43 2.318140E-02 4.778840E+00 4.620854E-01 1295 44 2.360130E-02 4.666560E+00 4.428954E-01 1295 45 2.402110E-02 4.223360E+00 4.431564E-01 1295 46 2.444090E-02 4.423360E+00 4.387864E-01 1295 47 2.486070E-02 4.421240E+00 4.149432E-01 1295 48 2.528060E-02 3.703360E+00 4.181298E-01 1295 49 2.570040E-02 3.966220E+00 4.113928E-01 1295 50 2.612020E-02 3.828460E+00 4.109400E-01 1295 51 2.654010E-02 3.648840E+00 3.925396E-01 1295 52 2.695990E-02 3.633900E+00 3.972700E-01 1295 53 2.737970E-02 3.132080E+00 3.829954E-01 1295 54 2.779960E-02 3.376880E+00 3.780394E-01 1295 55 2.821940E-02 2.750140E+00 3.819680E-01 1295 56 2.863920E-02 2.999460E+00 3.770654E-01 1295 57 2.905900E-02 2.521420E+00 3.845180E-01 1295 58 2.947890E-02 2.698000E+00 3.843288E-01 1295 59 2.989870E-02 2.900300E+00 3.705628E-01 1295 60 3.031850E-02 2.915540E+00 3.744520E-01 1295 61 3.073840E-02 2.401060E+00 3.729400E-01 1295 62 3.115820E-02 2.320440E+00 3.687650E-01 1295 63 3.157800E-02 2.660620E+00 3.848040E-01 1295 64 3.199790E-02 2.353460E+00 3.724692E-01 1295 65 3.241770E-02 2.314100E+00 3.754928E-01 1295 66 3.283750E-02 2.038760E+00 3.681916E-01 1295 67 3.325730E-02 2.101920E+00 3.615220E-01 1295 68 3.367720E-02 2.057460E+00 3.513240E-01 1295 69 3.409700E-02 1.999700E+00 3.511756E-01 1295 70 3.451680E-02 1.848240E+00 3.676900E-01 1295 71 3.493670E-02 1.990360E+00 3.403206E-01 1295 72 3.535650E-02 2.166980E+00 3.475518E-01 1295 73 3.577630E-02 1.827840E+00 3.391184E-01 1295 74 3.619620E-02 1.812180E+00 3.410690E-01 1295 75 3.661600E-02 1.851220E+00 3.385698E-01 1295 76 3.703580E-02 1.256400E+00 3.278708E-01 1295 77 3.745560E-02 1.686980E+00 3.360924E-01 1295 78 3.787550E-02 1.572380E+00 3.150266E-01 1295 79 3.829530E-02 1.645060E+00 3.180780E-01 1295 80 3.871510E-02 1.273420E+00 3.177144E-01 1295 81 3.913500E-02 1.708760E+00 3.126780E-01 1295 82 3.955480E-02 1.735800E+00 3.184442E-01 1295 83 3.997460E-02 1.499900E+00 3.081292E-01 1295 84 4.039450E-02 1.272120E+00 3.039158E-01 1295 85 4.081430E-02 1.537180E+00 3.077684E-01 1295 86 4.123410E-02 1.302400E+00 3.007102E-01 1295 87 4.165390E-02 1.170740E+00 3.009522E-01 1295 88 4.207380E-02 1.382400E+00 2.921406E-01 1295 89 4.249360E-02 1.197840E+00 2.930386E-01 1295 90 4.291340E-02 1.222240E+00 2.894716E-01 1295 91 4.333330E-02 1.025920E+00 2.796528E-01 1295 92 4.375310E-02 1.266620E+00 2.840578E-01 1295 93 4.417290E-02 1.301300E+00 2.752764E-01 1295 94 4.459280E-02 9.408996E-01 2.765844E-01 1295 95 4.501260E-02 1.182860E+00 2.702532E-01 1295 96 4.543240E-02 1.113200E+00 2.654456E-01 1295 97 4.585220E-02 1.202760E+00 2.633468E-01 1295 98 4.627210E-02 1.102380E+00 2.681630E-01 1295 99 4.669190E-02 9.919400E-01 2.688510E-01 1295 100 4.711170E-02 1.149840E+00 2.672258E-01 1295 101 4.753160E-02 1.008140E+00 2.594330E-01 1295 102 4.795140E-02 1.170700E+00 2.610552E-01 1295 103 4.837120E-02 9.865202E-01 2.517576E-01 1295 104 4.879110E-02 1.080160E+00 2.648234E-01 1295 105 4.921090E-02 9.332800E-01 2.569116E-01 1295 106 4.963070E-02 7.969200E-01 2.554228E-01 1295 107 5.005050E-02 9.799596E-01 2.510162E-01 1295 108 5.047040E-02 1.026220E+00 2.524306E-01 1295 109 5.089020E-02 1.001760E+00 2.531210E-01 1295 110 5.131000E-02 1.064740E+00 2.501618E-01 1295 111 5.172990E-02 7.916404E-01 2.515418E-01 1295 112 5.214970E-02 9.368196E-01 2.475094E-01 1295 113 5.256950E-02 8.609200E-01 2.429734E-01 1295 114 5.298940E-02 7.598004E-01 2.386196E-01 1295 115 5.340920E-02 7.595800E-01 2.428158E-01 1295 116 5.382900E-02 8.626198E-01 2.473500E-01 1295 117 5.424890E-02 7.289392E-01 2.394596E-01 1295 118 5.466870E-02 6.476000E-01 2.458728E-01 1295 119 5.508850E-02 7.607400E-01 2.380766E-01 1295 120 5.550830E-02 7.842200E-01 2.353412E-01 1295 121 5.592820E-02 7.132802E-01 2.339348E-01 1295 122 5.634800E-02 7.225198E-01 2.347082E-01 1295 123 5.676780E-02 7.121200E-01 2.397236E-01 1295 124 5.718770E-02 8.150598E-01 2.304988E-01 1295 125 5.760750E-02 8.000398E-01 2.336116E-01 1295 126 5.802730E-02 8.244196E-01 2.296860E-01 1295 127 5.844720E-02 8.062198E-01 2.256658E-01 1295 128 5.886700E-02 8.446402E-01 2.314610E-01 1295 129 5.928680E-02 6.436802E-01 2.224446E-01 1295 130 5.970660E-02 5.423598E-01 2.228906E-01 1295 131 6.012650E-02 8.050606E-01 2.244936E-01 1295 132 6.054630E-02 6.635400E-01 2.258008E-01 1295 133 6.096610E-02 5.077598E-01 2.269134E-01 1295 134 6.138600E-02 6.703000E-01 2.244266E-01 1295 135 6.180580E-02 5.898800E-01 2.275804E-01 1295 136 6.222560E-02 5.768194E-01 2.198688E-01 1295 137 6.264550E-02 7.239398E-01 2.142314E-01 1295 138 6.306530E-02 5.063202E-01 2.174312E-01 1295 139 6.348510E-02 6.812999E-01 2.128140E-01 1295 140 6.390490E-02 5.699602E-01 2.218752E-01 1295 141 6.432480E-02 6.580400E-01 2.167416E-01 1295 142 6.474460E-02 6.111398E-01 2.145970E-01 1295 143 6.516440E-02 5.938404E-01 2.141410E-01 1295 144 6.558430E-02 6.195602E-01 2.150600E-01 1295 145 6.600410E-02 5.508408E-01 2.166170E-01 1295 146 6.642390E-02 4.530602E-01 2.114952E-01 1295 147 6.684380E-02 6.256204E-01 2.144942E-01 1295 148 6.726360E-02 3.780000E-01 2.058018E-01 1295 149 6.768340E-02 5.236600E-01 2.060392E-01 1295 150 6.810320E-02 6.405398E-01 2.081372E-01 1295 151 6.852310E-02 6.139602E-01 2.040420E-01 1295 152 6.894290E-02 4.688002E-01 2.098568E-01 1295 153 6.936270E-02 5.111800E-01 2.047264E-01 1295 154 6.978260E-02 5.518400E-01 2.091598E-01 1295 155 7.020240E-02 6.208596E-01 2.061324E-01 1295 156 7.062220E-02 5.487402E-01 2.009180E-01 1295 157 7.104210E-02 5.230200E-01 2.037260E-01 1295 158 7.146190E-02 5.514396E-01 2.054856E-01 1295 159 7.188170E-02 5.371798E-01 2.023466E-01 1295 160 7.230160E-02 5.551600E-01 2.014650E-01 1295 161 7.272140E-02 6.178204E-01 2.003098E-01 1295 162 7.314120E-02 4.551996E-01 2.020792E-01 1295 163 7.356100E-02 3.564600E-01 1.974606E-01 1295 164 7.398090E-02 4.500204E-01 2.003676E-01 1295 165 7.440070E-02 4.443198E-01 1.945518E-01 1295 166 7.482050E-02 5.641400E-01 1.981164E-01 1295 167 7.524040E-02 4.294000E-01 1.935320E-01 1295 168 7.566020E-02 3.984804E-01 1.965882E-01 1295 169 7.608000E-02 4.321402E-01 1.997020E-01 1295 170 7.649980E-02 4.250800E-01 1.924188E-01 1295 171 7.691970E-02 3.004798E-01 1.930970E-01 1295 172 7.733950E-02 4.103402E-01 1.936320E-01 1295 173 7.775930E-02 4.724998E-01 1.910392E-01 1295 174 7.817920E-02 5.122800E-01 1.984150E-01 1295 175 7.859900E-02 5.123800E-01 1.901282E-01 1295 176 7.901880E-02 3.564596E-01 1.941774E-01 1295 177 7.943870E-02 3.968000E-01 1.931392E-01 1295 178 7.985850E-02 3.610606E-01 1.924324E-01 1295 179 8.027830E-02 2.475803E-01 1.905878E-01 1295 180 8.069810E-02 2.826198E-01 1.903906E-01 1295 181 8.111800E-02 3.314800E-01 1.950430E-01 1295 182 8.153780E-02 4.203808E-01 1.922734E-01 1295 183 8.195760E-02 5.764196E-01 1.906522E-01 1295 184 8.237750E-02 3.431602E-01 1.899908E-01 1295 185 8.279730E-02 2.931797E-01 1.916494E-01 1295 186 8.321710E-02 4.469996E-01 1.895486E-01 1295 187 8.363700E-02 2.556799E-01 1.904688E-01 1295 188 8.405680E-02 2.361602E-01 1.993536E-01 1295 189 8.447660E-02 1.904004E-01 1.994272E-01 1295 190 8.489650E-02 3.905202E-01 1.986430E-01 1295 191 8.531630E-02 4.324800E-01 2.020676E-01 1295 192 8.573610E-02 3.589204E-01 2.014410E-01 1295 193 8.615590E-02 4.428396E-01 2.093356E-01 1295 194 8.657580E-02 4.110404E-01 2.008762E-01 1295 195 8.699560E-02 3.621001E-01 2.064892E-01 1295 196 8.741540E-02 4.308394E-01 2.019884E-01 1295 197 8.783530E-02 3.132397E-01 2.037368E-01 1295 198 8.825510E-02 3.279198E-01 2.098952E-01 1295 199 8.867490E-02 -2.160000E-02 2.099568E-01 1295 200 8.909480E-02 2.568392E-01 2.031438E-01 1295 201 8.951460E-02 3.393196E-01 2.054632E-01 1295 202 8.993440E-02 4.423202E-01 2.056610E-01 1295 203 9.035420E-02 3.508998E-01 2.044406E-01 1295 204 9.077410E-02 3.916400E-01 2.024220E-01 1295 205 9.119390E-02 3.957006E-01 2.080938E-01 1295 206 9.161370E-02 3.093404E-01 2.081072E-01 1295 207 9.203360E-02 2.440999E-01 2.101812E-01 1295 208 9.245340E-02 4.185202E-01 2.125862E-01 1295 209 9.287320E-02 4.180000E-01 2.146920E-01 1295 210 9.329310E-02 3.914004E-01 2.112286E-01 1295 211 9.371290E-02 2.735800E-01 2.139856E-01 1295 212 9.413270E-02 3.162406E-01 2.153392E-01 1295 213 9.455250E-02 2.704204E-01 2.113240E-01 1295 214 9.497240E-02 4.795600E-01 2.084170E-01 1295 215 9.539220E-02 3.271199E-01 2.036448E-01 1295 216 9.581200E-02 3.357801E-01 2.045936E-01 1295 217 9.623190E-02 2.882401E-01 2.070252E-01 1295 218 9.665170E-02 1.521595E-01 2.045552E-01 1295 219 9.707150E-02 4.008597E-01 2.065336E-01 1295 220 9.749140E-02 2.262401E-01 2.024450E-01 1295 221 9.791120E-02 3.061206E-01 1.970880E-01 1295 222 9.833100E-02 2.835396E-01 2.038980E-01 1295 223 9.875080E-02 1.750797E-01 1.956420E-01 1295 224 9.917070E-02 2.783596E-01 1.999974E-01 1295 225 9.959050E-02 2.355405E-01 2.019680E-01 1295 226 1.000100E-01 3.402004E-01 1.982086E-01 1295 227 1.004300E-01 1.776399E-01 1.966104E-01 1295 228 1.008500E-01 2.849601E-01 1.925972E-01 1295 229 1.012700E-01 2.530201E-01 1.985114E-01 1295 230 1.016900E-01 2.337199E-01 1.977154E-01 1295 231 1.021090E-01 3.851404E-01 1.991550E-01 1295 232 1.025290E-01 1.118392E-01 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