{ "code": "SASDAG5", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq.", "publication": { "title": "Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq.", "author_list": "Beich-Frandsen M, Vecerek B, Konarev PV, Sjöblom B, Kloiber K, Hämmerle H, Rajkowitsch L, Miles AJ, Kontaxis G, Wallace BA, Svergun DI, Konrat R, Bläsi U, Djinovic-Carugo K", "journal": "Nucleic Acids Res", "doi": "10.1093/nar/gkq1346", "pmid": "21330354", "published_date": "2011 Jun" }, "status": "released", "submitted_date": null, "released_date": "2015-07-07" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDAG5.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAG5.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAG5_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAG5_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAG5_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAG5_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDAG5.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "MAR 345 Image Plate", "resolution": null }, "name": "DORIS III, DESY", "city": "Hamburg", "country": "Germany", "beamline_name": "EMBL X33", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "RNA chaperone Hfq", "short_name": "Hfq", "sequence": "MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTS\r\nSNYHHGSSAQNTSAQQDSEETE", "organism": "Escherichia coli", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "hexamer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 11.2, "total_mw": 67.2, "number_molecules": 6, "complex_state": null, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT", "concentration_unit": null, "comment": null, "additive": "150 mM NaCl, 1.0 mM DTT", "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "RNA shaperone Hfq", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Petr", "contributor_surname": "Konarev", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2008-05-02", "storage_temperature": 37.0, "cell_temperature": 37.0, "exposure_time": 120.0, "number_of_frames": 2, "wavelength": null, "sample_detector_distance": null, "concentration_min": 2.3, "concentration_max": 18.5, "sample_volume": null, "flow_rate": null, "s_min": 0.155, "s_max": 5.171, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAG5_fit1_model1_img.png", "software": "DAMMIN", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAG5_fit1_model1.pdb", "model_mw": null, "bead_radius": 1.9, "log": null, "symmetry": "P6", "comment": "", "user": 88 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAG5_fit1_fit_img.png", "software": null, "chi_square_value": 6.853924, "p_value": 0.0, "fit_residual_plot": "SASDAG5_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDAG5_fit1.fir", "fit_log": null, "software_version": null, "description": null }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAG5_fit2_model1_img.png", "software": "BUNCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAG5_fit2_model1.pdb", "model_mw": 46.93, "bead_radius": 1.9, "log": null, "symmetry": "P6", "comment": "", "user": 88 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAG5_fit2_fit_img.png", "software": null, "chi_square_value": 4.9284, "p_value": 0.0, "fit_residual_plot": "SASDAG5_fit2_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDAG5_fit2.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "GNOM", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [ "X33" ], "intensity_unit": null, "experimental_mw": 67.2, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 133.0, "pddf_i0_error": null, "guinier_i0": 130.0, "guinier_i0_error": null, "pddf_rg": 3.23, "pddf_rg_error": null, "guinier_rg": 3.2, "guinier_rg_error": null, "pddf_dmax": 11.2, "pddf_dmax_error": null, "porod_volume": 110.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": null, "guinier_point_last": null, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDAG5_datloglog_img.png", "symmetry": null, "last_modified": "2023-05-25T11:22:16.556436+02:00", "bragg_peak": [] }