{ "code": "SASDCG7", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques.", "publication": { "title": "Characterizing Protein Dynamics with Integrative Use of Bulk and Single-Molecule Techniques.", "author_list": "Liu Z, Gong Z, Cao Y, Ding YH, Dong MQ, Lu YB, Zhang WP, Tang C", "journal": "Biochemistry", "doi": "10.1021/acs.biochem.7b00817", "pmid": "28945353", "published_date": "2018 Jan 23" }, "status": "released", "submitted_date": "2017-09-12", "released_date": "2017-09-29" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCG7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCG7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCG7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCG7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCG7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCG7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCG7.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "Shanghai Synchrotron Radiation Facility (SSRF)", "city": "Shanghai", "country": "China", "beamline_name": "BL19U2", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Polyubiquitin-C", "short_name": "ubiquitin", "sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG", "organism": "Homo sapiens", "uniprot_code": "P0CG48", "uniprot_range_first": 305, "uniprot_range_last": 379, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN\nIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI\nFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA\nKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT\nITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR\nLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL\nSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ\nQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE\nNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL\nTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH\nLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED\nGRTLSDYNIQKESTLHLVLRLRGGV", "mw": 8.565, "total_mw": 17.13, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20mM HEPES, 150mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Lys63-linked diubiquitin at pH7.4", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Wuhan, China", "full_name": "Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences", "webpage": "http://english.wipm.cas.cn/" } ], "contributor_name": "Zhu", "contributor_surname": "Liuzhu", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2016-03-24", "storage_temperature": 25.0, "cell_temperature": 25.0, "exposure_time": 1.0, "number_of_frames": 20, "wavelength": 0.1033, "sample_detector_distance": 2.1, "concentration_min": null, "concentration_max": 2.6, "sample_volume": null, "flow_rate": null, "s_min": 0.197, "s_max": 4.611, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of Lys63-linked diubiquitin in 20mM HEPES, 150mM NaCl, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facilitystorage ring (SSRF: Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). One solute concentration of 2.60 mg/ml (150 µM) was measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": null, "experimental_mw": 13.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 13.0, "porod_mw_error": null, "pddf_i0": 66.4, "pddf_i0_error": 0.56, "guinier_i0": 63.25, "guinier_i0_error": 1.64, "pddf_rg": 2.1, "pddf_rg_error": 0.01, "guinier_rg": 2.0, "guinier_rg_error": 0.15, "pddf_dmax": 7.0, "pddf_dmax_error": null, "porod_volume": 22.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 4, "guinier_point_last": 55, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCG7_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }