{
  "code": "SASDDH2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "C<sub>H</sub>2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.",
    "publication": {
      "title": "C<sub>H</sub>2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.",
      "author_list": "Yageta S, Imamura H, Shibuya R, Honda S",
      "journal": "MAbs",
      "doi": "10.1080/19420862.2018.1546086",
      "pmid": "30513259",
      "published_date": "2018 Dec 4"
    },
    "status": "released",
    "submitted_date": "2018-12-08",
    "released_date": "2018-12-09"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDH2.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDH2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDH2_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDH2_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDH2.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "Photon Factory (PF), High Energy Accelerator Research Organization (KEK)",
      "city": "Tsukuba",
      "country": "Japan",
      "beamline_name": "BL-10C",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Aglycosylated human immunoglobulin G Fc region",
          "short_name": "aFc",
          "sequence": "ADKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK",
          "organism": "Homo sapiens",
          "uniprot_code": "P01857",
          "uniprot_range_first": 104,
          "uniprot_range_last": 330,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS\nGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG\nPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYN\nSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDE\nLTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW\nQQGNVFSCSVMHEALHNHYTQKSLSLSPGK",
          "mw": 25.651,
          "total_mw": 51.302,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM Citrate-Phosphate",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Aglycosylated Human Immunoglobulin G Fc Region",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "AIST",
            "address": "Tokyo, Japan",
            "full_name": "National Institute of Advanced Industrial Science and Technology (AIST)",
            "webpage": "http://www.aist.go.jp"
          }
        ],
        "contributor_name": "Seiki",
        "contributor_surname": "Yageta",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-03-05",
    "storage_temperature": null,
    "cell_temperature": 25.0,
    "exposure_time": 2.0,
    "number_of_frames": 15,
    "wavelength": 0.12,
    "sample_detector_distance": 2.0,
    "concentration_min": null,
    "concentration_max": 3.19,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.056,
    "s_max": 3.497,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDH2_fit1_model1_img.png",
          "software": "MODELLER",
          "pdb_link": [
            {
              "pdb_code": "1hzh",
              "ext_code": "1HZH",
              "ext_type": "pdb",
              "difference_with_model": "derived"
            }
          ],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDH2_fit1_model1.pdb",
          "model_mw": 51.24,
          "bead_radius": null,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 270
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDH2_fit1_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 0.026,
      "p_value": 0.010176,
      "fit_residual_plot": "SASDDH2_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDH2_fit1.fit",
      "fit_log": null,
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of aglycosylated Human Immunoglobulin G (Fc Region) in 20 mM Citrate-Phosphate, pH 7 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring (Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.19 mg/ml was measured at 25°C. 15 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 51.297,
  "experimental_mw_error": null,
  "guinier_i0_mw": 50.6,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 1849.0,
  "pddf_i0_error": null,
  "guinier_i0": 0.036,
  "guinier_i0_error": 0.0,
  "pddf_rg": 2.85,
  "pddf_rg_error": null,
  "guinier_rg": 2.87,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 9.8,
  "pddf_dmax_error": null,
  "porod_volume": 60.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 39,
  "guinier_point_last": 253,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDDH2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}