data_SASDFH2_MAIN # _sas_result.id 1296 _sas_result.SASBDB_code SASDFH2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 29.5000000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 10.460 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 3 _sas_result.Guinier_point_max 16 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 255-259 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259)" _sas_scan.detc_id 15 _sas_scan.id 1296 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1257 _sas_scan.result_id 1296 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1257 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 255-259)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1257 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 2.665780E+01 1.388208E+01 1296 2 5.968380E-03 3.340680E+01 8.739244E+00 1296 3 6.388220E-03 2.595620E+01 6.091330E+00 1296 4 6.808050E-03 2.449500E+01 4.707304E+00 1296 5 7.227880E-03 2.370220E+01 4.007316E+00 1296 6 7.647710E-03 2.327500E+01 3.290344E+00 1296 7 8.067540E-03 2.680940E+01 2.902022E+00 1296 8 8.487370E-03 2.056800E+01 2.469178E+00 1296 9 8.907200E-03 2.057498E+01 2.109380E+00 1296 10 9.327030E-03 2.200078E+01 1.997722E+00 1296 11 9.746860E-03 2.133176E+01 1.835152E+00 1296 12 1.016670E-02 1.972728E+01 1.691596E+00 1296 13 1.058650E-02 1.927352E+01 1.581280E+00 1296 14 1.100630E-02 1.922132E+01 1.545890E+00 1296 15 1.142620E-02 1.846498E+01 1.416004E+00 1296 16 1.184600E-02 1.769180E+01 1.263086E+00 1296 17 1.226580E-02 1.677558E+01 1.249674E+00 1296 18 1.268570E-02 1.655908E+01 1.138638E+00 1296 19 1.310550E-02 1.596974E+01 1.033462E+00 1296 20 1.352530E-02 1.422434E+01 1.073682E+00 1296 21 1.394520E-02 1.391454E+01 9.475870E-01 1296 22 1.436500E-02 1.314374E+01 9.817380E-01 1296 23 1.478480E-02 1.347308E+01 9.296260E-01 1296 24 1.520460E-02 1.232912E+01 8.883312E-01 1296 25 1.562450E-02 1.271080E+01 7.973580E-01 1296 26 1.604430E-02 1.156188E+01 7.876166E-01 1296 27 1.646410E-02 1.084074E+01 7.000764E-01 1296 28 1.688400E-02 1.007568E+01 6.815178E-01 1296 29 1.730380E-02 9.732740E+00 7.093500E-01 1296 30 1.772360E-02 1.023942E+01 6.394284E-01 1296 31 1.814350E-02 9.517580E+00 6.422806E-01 1296 32 1.856330E-02 9.039720E+00 6.218078E-01 1296 33 1.898310E-02 8.766580E+00 5.821070E-01 1296 34 1.940290E-02 8.529800E+00 5.863984E-01 1296 35 1.982280E-02 7.572200E+00 5.655390E-01 1296 36 2.024260E-02 7.658340E+00 5.445492E-01 1296 37 2.066240E-02 7.636880E+00 5.160706E-01 1296 38 2.108230E-02 6.884320E+00 5.344286E-01 1296 39 2.150210E-02 7.039320E+00 5.226312E-01 1296 40 2.192190E-02 6.461440E+00 4.974898E-01 1296 41 2.234180E-02 6.307500E+00 4.897118E-01 1296 42 2.276160E-02 5.805580E+00 4.681292E-01 1296 43 2.318140E-02 5.437620E+00 4.627482E-01 1296 44 2.360130E-02 5.384980E+00 4.339224E-01 1296 45 2.402110E-02 5.269240E+00 4.284338E-01 1296 46 2.444090E-02 5.068100E+00 4.353042E-01 1296 47 2.486070E-02 4.885860E+00 4.289452E-01 1296 48 2.528060E-02 4.579560E+00 4.318298E-01 1296 49 2.570040E-02 4.578460E+00 4.285376E-01 1296 50 2.612020E-02 4.499400E+00 4.095216E-01 1296 51 2.654010E-02 3.881900E+00 4.036372E-01 1296 52 2.695990E-02 4.364220E+00 4.065100E-01 1296 53 2.737970E-02 3.971520E+00 4.028670E-01 1296 54 2.779960E-02 3.723460E+00 3.766086E-01 1296 55 2.821940E-02 3.679740E+00 3.935298E-01 1296 56 2.863920E-02 3.574760E+00 3.952852E-01 1296 57 2.905900E-02 3.516440E+00 3.824424E-01 1296 58 2.947890E-02 3.338800E+00 3.914338E-01 1296 59 2.989870E-02 3.439540E+00 3.749080E-01 1296 60 3.031850E-02 3.267080E+00 3.918860E-01 1296 61 3.073840E-02 2.836980E+00 3.853074E-01 1296 62 3.115820E-02 2.880660E+00 3.769210E-01 1296 63 3.157800E-02 3.200180E+00 3.797642E-01 1296 64 3.199790E-02 2.477960E+00 3.562420E-01 1296 65 3.241770E-02 2.639820E+00 3.652886E-01 1296 66 3.283750E-02 2.630680E+00 3.663996E-01 1296 67 3.325730E-02 2.463520E+00 3.711848E-01 1296 68 3.367720E-02 2.573440E+00 3.760690E-01 1296 69 3.409700E-02 2.503280E+00 3.444668E-01 1296 70 3.451680E-02 2.392780E+00 3.673478E-01 1296 71 3.493670E-02 2.207640E+00 3.440280E-01 1296 72 3.535650E-02 2.391940E+00 3.578988E-01 1296 73 3.577630E-02 2.495280E+00 3.483394E-01 1296 74 3.619620E-02 1.978780E+00 3.424610E-01 1296 75 3.661600E-02 2.026320E+00 3.417270E-01 1296 76 3.703580E-02 2.165240E+00 3.390246E-01 1296 77 3.745560E-02 1.782340E+00 3.337182E-01 1296 78 3.787550E-02 1.820940E+00 3.238300E-01 1296 79 3.829530E-02 1.811240E+00 3.098596E-01 1296 80 3.871510E-02 1.551100E+00 3.209684E-01 1296 81 3.913500E-02 1.910940E+00 3.192930E-01 1296 82 3.955480E-02 1.819960E+00 3.279176E-01 1296 83 3.997460E-02 1.652160E+00 3.130910E-01 1296 84 4.039450E-02 1.766000E+00 3.110010E-01 1296 85 4.081430E-02 1.446040E+00 3.025532E-01 1296 86 4.123410E-02 1.602240E+00 2.951584E-01 1296 87 4.165390E-02 1.711520E+00 3.097252E-01 1296 88 4.207380E-02 1.520660E+00 2.921712E-01 1296 89 4.249360E-02 1.703320E+00 2.973886E-01 1296 90 4.291340E-02 1.269260E+00 2.908870E-01 1296 91 4.333330E-02 1.395540E+00 2.912780E-01 1296 92 4.375310E-02 1.625300E+00 2.924542E-01 1296 93 4.417290E-02 1.227100E+00 2.723476E-01 1296 94 4.459280E-02 1.490520E+00 2.757756E-01 1296 95 4.501260E-02 1.507520E+00 2.728300E-01 1296 96 4.543240E-02 1.304820E+00 2.782538E-01 1296 97 4.585220E-02 1.178560E+00 2.724006E-01 1296 98 4.627210E-02 1.208100E+00 2.628410E-01 1296 99 4.669190E-02 1.386800E+00 2.754008E-01 1296 100 4.711170E-02 1.201080E+00 2.684092E-01 1296 101 4.753160E-02 1.243180E+00 2.617532E-01 1296 102 4.795140E-02 9.817600E-01 2.615364E-01 1296 103 4.837120E-02 1.178760E+00 2.534296E-01 1296 104 4.879110E-02 1.067040E+00 2.584604E-01 1296 105 4.921090E-02 1.039760E+00 2.542528E-01 1296 106 4.963070E-02 9.143200E-01 2.569432E-01 1296 107 5.005050E-02 1.094080E+00 2.537502E-01 1296 108 5.047040E-02 1.092200E+00 2.495676E-01 1296 109 5.089020E-02 8.520200E-01 2.509088E-01 1296 110 5.131000E-02 9.947796E-01 2.483378E-01 1296 111 5.172990E-02 8.630200E-01 2.523844E-01 1296 112 5.214970E-02 8.413198E-01 2.520940E-01 1296 113 5.256950E-02 9.990600E-01 2.586952E-01 1296 114 5.298940E-02 8.009602E-01 2.414716E-01 1296 115 5.340920E-02 8.023600E-01 2.412616E-01 1296 116 5.382900E-02 8.358196E-01 2.379978E-01 1296 117 5.424890E-02 1.011840E+00 2.409286E-01 1296 118 5.466870E-02 1.041180E+00 2.458754E-01 1296 119 5.508850E-02 6.892798E-01 2.333578E-01 1296 120 5.550830E-02 8.936400E-01 2.390386E-01 1296 121 5.592820E-02 1.077940E+00 2.348202E-01 1296 122 5.634800E-02 9.048998E-01 2.377908E-01 1296 123 5.676780E-02 9.993602E-01 2.412738E-01 1296 124 5.718770E-02 7.440598E-01 2.288884E-01 1296 125 5.760750E-02 9.455600E-01 2.378500E-01 1296 126 5.802730E-02 9.152794E-01 2.320230E-01 1296 127 5.844720E-02 9.983398E-01 2.287658E-01 1296 128 5.886700E-02 6.784008E-01 2.259206E-01 1296 129 5.928680E-02 7.415404E-01 2.255786E-01 1296 130 5.970660E-02 7.859996E-01 2.361068E-01 1296 131 6.012650E-02 8.167202E-01 2.267146E-01 1296 132 6.054630E-02 5.971200E-01 2.269636E-01 1296 133 6.096610E-02 6.115398E-01 2.249692E-01 1296 134 6.138600E-02 6.055200E-01 2.180818E-01 1296 135 6.180580E-02 9.006800E-01 2.251968E-01 1296 136 6.222560E-02 6.614400E-01 2.222146E-01 1296 137 6.264550E-02 8.638598E-01 2.178788E-01 1296 138 6.306530E-02 7.349600E-01 2.199744E-01 1296 139 6.348510E-02 6.886400E-01 2.142922E-01 1296 140 6.390490E-02 8.220200E-01 2.210592E-01 1296 141 6.432480E-02 7.429198E-01 2.156866E-01 1296 142 6.474460E-02 7.487000E-01 2.150484E-01 1296 143 6.516440E-02 5.485602E-01 2.130388E-01 1296 144 6.558430E-02 6.427200E-01 2.118306E-01 1296 145 6.600410E-02 6.955602E-01 2.186584E-01 1296 146 6.642390E-02 5.654402E-01 2.081068E-01 1296 147 6.684380E-02 7.125002E-01 2.183540E-01 1296 148 6.726360E-02 7.486000E-01 2.088556E-01 1296 149 6.768340E-02 5.408800E-01 2.112494E-01 1296 150 6.810320E-02 7.122798E-01 2.110700E-01 1296 151 6.852310E-02 6.472806E-01 2.041542E-01 1296 152 6.894290E-02 5.687002E-01 2.139342E-01 1296 153 6.936270E-02 7.078800E-01 2.051986E-01 1296 154 6.978260E-02 6.024200E-01 2.085144E-01 1296 155 7.020240E-02 3.387793E-01 2.011678E-01 1296 156 7.062220E-02 5.861002E-01 2.016784E-01 1296 157 7.104210E-02 4.895802E-01 2.065140E-01 1296 158 7.146190E-02 5.773794E-01 2.031392E-01 1296 159 7.188170E-02 5.878394E-01 2.023884E-01 1296 160 7.230160E-02 6.322000E-01 2.000224E-01 1296 161 7.272140E-02 6.414802E-01 2.068804E-01 1296 162 7.314120E-02 6.483798E-01 1.989316E-01 1296 163 7.356100E-02 5.414004E-01 1.957490E-01 1296 164 7.398090E-02 3.993000E-01 1.973188E-01 1296 165 7.440070E-02 4.820000E-01 1.953872E-01 1296 166 7.482050E-02 5.500204E-01 1.982802E-01 1296 167 7.524040E-02 5.010800E-01 1.963538E-01 1296 168 7.566020E-02 6.522404E-01 1.950780E-01 1296 169 7.608000E-02 4.775604E-01 1.938032E-01 1296 170 7.649980E-02 5.428000E-01 1.998390E-01 1296 171 7.691970E-02 6.292996E-01 1.976428E-01 1296 172 7.733950E-02 4.684600E-01 1.964206E-01 1296 173 7.775930E-02 4.505992E-01 1.945278E-01 1296 174 7.817920E-02 5.927600E-01 1.938802E-01 1296 175 7.859900E-02 3.686801E-01 1.906036E-01 1296 176 7.901880E-02 3.071199E-01 1.944522E-01 1296 177 7.943870E-02 5.368400E-01 1.917646E-01 1296 178 7.985850E-02 4.361604E-01 1.918964E-01 1296 179 8.027830E-02 4.862600E-01 1.938956E-01 1296 180 8.069810E-02 5.611998E-01 1.958300E-01 1296 181 8.111800E-02 5.548600E-01 1.934578E-01 1296 182 8.153780E-02 3.299000E-01 1.895060E-01 1296 183 8.195760E-02 5.727396E-01 1.943820E-01 1296 184 8.237750E-02 5.656600E-01 1.910794E-01 1296 185 8.279730E-02 3.914400E-01 1.881376E-01 1296 186 8.321710E-02 5.095796E-01 1.908484E-01 1296 187 8.363700E-02 3.840400E-01 1.934276E-01 1296 188 8.405680E-02 4.174000E-01 1.960278E-01 1296 189 8.447660E-02 4.842604E-01 2.031336E-01 1296 190 8.489650E-02 3.938800E-01 2.058692E-01 1296 191 8.531630E-02 4.115204E-01 2.047016E-01 1296 192 8.573610E-02 3.151806E-01 2.002710E-01 1296 193 8.615590E-02 2.642596E-01 2.058164E-01 1296 194 8.657580E-02 2.293605E-01 2.036274E-01 1296 195 8.699560E-02 4.018603E-01 2.022948E-01 1296 196 8.741540E-02 4.310198E-01 2.083008E-01 1296 197 8.783530E-02 3.330196E-01 2.014848E-01 1296 198 8.825510E-02 2.450600E-01 2.068922E-01 1296 199 8.867490E-02 4.049200E-01 2.122278E-01 1296 200 8.909480E-02 3.804994E-01 2.072882E-01 1296 201 8.951460E-02 3.586797E-01 2.027144E-01 1296 202 8.993440E-02 3.710200E-01 2.029892E-01 1296 203 9.035420E-02 5.319196E-01 2.104480E-01 1296 204 9.077410E-02 4.374600E-01 2.074254E-01 1296 205 9.119390E-02 2.287001E-01 2.088686E-01 1296 206 9.161370E-02 6.312402E-01 2.057600E-01 1296 207 9.203360E-02 4.878998E-01 2.127746E-01 1296 208 9.245340E-02 2.314008E-01 2.058470E-01 1296 209 9.287320E-02 4.404404E-01 2.101132E-01 1296 210 9.329310E-02 4.018206E-01 2.118488E-01 1296 211 9.371290E-02 2.473404E-01 2.162766E-01 1296 212 9.413270E-02 3.148602E-01 2.069342E-01 1296 213 9.455250E-02 2.163202E-01 2.114716E-01 1296 214 9.497240E-02 2.410593E-01 2.108112E-01 1296 215 9.539220E-02 3.398202E-01 2.074164E-01 1296 216 9.581200E-02 3.359802E-01 2.049626E-01 1296 217 9.623190E-02 3.493999E-01 2.089032E-01 1296 218 9.665170E-02 3.320394E-01 2.005190E-01 1296 219 9.707150E-02 4.326998E-01 2.060276E-01 1296 220 9.749140E-02 4.156004E-01 2.049622E-01 1296 221 9.791120E-02 4.281802E-01 1.979674E-01 1296 222 9.833100E-02 2.483198E-01 2.003128E-01 1296 223 9.875080E-02 2.441393E-01 1.969020E-01 1296 224 9.917070E-02 2.074197E-01 1.948758E-01 1296 225 9.959050E-02 2.484406E-01 1.964030E-01 1296 226 1.000100E-01 7.326028E-02 1.952764E-01 1296 227 1.004300E-01 1.445798E-01 1.973808E-01 1296 228 1.008500E-01 4.080605E-01 1.944844E-01 1296 229 1.012700E-01 2.713006E-01 2.001184E-01 1296 230 1.016900E-01 1.030598E-01 1.926714E-01 1296 231 1.021090E-01 3.221604E-01 1.974780E-01 1296 232 1.025290E-01 2.736596E-01 1.945768E-01 1296 233 1.029490E-01 3.348002E-01 1.925968E-01 1296 234 1.033690E-01 2.469199E-01 1.936216E-01 1296 235 1.037890E-01 4.140594E-01 1.953872E-01 1296 236 1.042090E-01 4.069395E-01 1.954436E-01 1296 237 1.046280E-01 3.480400E-01 1.995950E-01 1296 238 1.050480E-01 2.484800E-01 1.936966E-01 1296 239 1.054680E-01 2.527000E-01 1.925900E-01 1296 240 1.058880E-01 1.970600E-01 1.872468E-01 1296 241 1.063080E-01 2.208206E-01 1.895482E-01 1296 242 1.067280E-01 3.219200E-01 1.929948E-01 1296 243 1.071470E-01 3.610998E-01 1.891600E-01 1296 244 1.075670E-01 2.202796E-01 1.876894E-01 1296 245 1.079870E-01 2.470403E-01 1.890582E-01 1296 246 1.084070E-01 2.810600E-01 1.909814E-01 1296 247 1.088270E-01 2.859796E-01 1.844852E-01 1296 248 1.092470E-01 2.062001E-01 1.886282E-01 1296 249 1.096660E-01 3.325600E-01 1.861180E-01 1296 250 1.100860E-01 2.494797E-01 1.876604E-01 1296 251 1.105060E-01 2.349196E-01 1.933444E-01 1296 252 1.109260E-01 3.040202E-01 1.889548E-01 1296 253 1.113460E-01 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