data_SASDFJ2_MAIN # _sas_result.id 1297 _sas_result.SASBDB_code SASDFJ2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 27.7500000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 10.140 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 4 _sas_result.Guinier_point_max 18 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 260-264 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)" _sas_scan.detc_id 15 _sas_scan.id 1297 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1258 _sas_scan.result_id 1297 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1258 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 260-264)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1258 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 9.198800E+00 1.278299E+01 1297 2 5.968380E-03 2.122020E+01 8.483512E+00 1297 3 6.388220E-03 2.727460E+01 6.138774E+00 1297 4 6.808050E-03 2.375140E+01 4.899824E+00 1297 5 7.227880E-03 2.256660E+01 3.795934E+00 1297 6 7.647710E-03 2.507500E+01 3.402400E+00 1297 7 8.067540E-03 2.392400E+01 2.815968E+00 1297 8 8.487370E-03 2.219100E+01 2.520952E+00 1297 9 8.907200E-03 2.064282E+01 2.082134E+00 1297 10 9.327030E-03 1.941298E+01 1.996472E+00 1297 11 9.746860E-03 1.973424E+01 1.768502E+00 1297 12 1.016670E-02 1.830006E+01 1.721944E+00 1297 13 1.058650E-02 1.908158E+01 1.586998E+00 1297 14 1.100630E-02 1.810170E+01 1.447760E+00 1297 15 1.142620E-02 1.759122E+01 1.320158E+00 1297 16 1.184600E-02 1.668168E+01 1.258852E+00 1297 17 1.226580E-02 1.784962E+01 1.269180E+00 1297 18 1.268570E-02 1.574120E+01 1.171866E+00 1297 19 1.310550E-02 1.427988E+01 1.099252E+00 1297 20 1.352530E-02 1.458686E+01 1.038262E+00 1297 21 1.394520E-02 1.390700E+01 9.705922E-01 1297 22 1.436500E-02 1.295270E+01 9.529554E-01 1297 23 1.478480E-02 1.287886E+01 8.454584E-01 1297 24 1.520460E-02 1.262408E+01 8.953556E-01 1297 25 1.562450E-02 1.179792E+01 8.126142E-01 1297 26 1.604430E-02 1.256096E+01 8.251882E-01 1297 27 1.646410E-02 1.120026E+01 7.479918E-01 1297 28 1.688400E-02 1.014946E+01 6.940888E-01 1297 29 1.730380E-02 1.015178E+01 6.760062E-01 1297 30 1.772360E-02 1.039772E+01 6.352552E-01 1297 31 1.814350E-02 1.034580E+01 6.725134E-01 1297 32 1.856330E-02 9.264520E+00 6.294800E-01 1297 33 1.898310E-02 8.842860E+00 5.786590E-01 1297 34 1.940290E-02 8.751120E+00 5.776594E-01 1297 35 1.982280E-02 8.488560E+00 5.672282E-01 1297 36 2.024260E-02 7.672940E+00 5.619004E-01 1297 37 2.066240E-02 7.639760E+00 5.271588E-01 1297 38 2.108230E-02 7.278820E+00 5.214166E-01 1297 39 2.150210E-02 7.067100E+00 5.124454E-01 1297 40 2.192190E-02 6.661640E+00 4.990552E-01 1297 41 2.234180E-02 6.372520E+00 5.001084E-01 1297 42 2.276160E-02 6.401680E+00 4.738016E-01 1297 43 2.318140E-02 5.899540E+00 4.813410E-01 1297 44 2.360130E-02 5.999020E+00 4.533014E-01 1297 45 2.402110E-02 5.539480E+00 4.376340E-01 1297 46 2.444090E-02 5.100220E+00 4.364086E-01 1297 47 2.486070E-02 5.378700E+00 4.377510E-01 1297 48 2.528060E-02 4.792320E+00 4.198016E-01 1297 49 2.570040E-02 4.890800E+00 4.196994E-01 1297 50 2.612020E-02 4.865740E+00 4.163570E-01 1297 51 2.654010E-02 4.449960E+00 4.052602E-01 1297 52 2.695990E-02 4.325600E+00 4.030170E-01 1297 53 2.737970E-02 4.027180E+00 4.062826E-01 1297 54 2.779960E-02 4.025320E+00 3.934992E-01 1297 55 2.821940E-02 3.658860E+00 4.041216E-01 1297 56 2.863920E-02 3.754100E+00 3.870974E-01 1297 57 2.905900E-02 3.685860E+00 3.764158E-01 1297 58 2.947890E-02 3.294980E+00 3.897980E-01 1297 59 2.989870E-02 3.610900E+00 3.801742E-01 1297 60 3.031850E-02 3.301300E+00 3.782134E-01 1297 61 3.073840E-02 3.274880E+00 3.898940E-01 1297 62 3.115820E-02 3.244040E+00 3.896224E-01 1297 63 3.157800E-02 3.035100E+00 3.833096E-01 1297 64 3.199790E-02 2.912240E+00 3.682708E-01 1297 65 3.241770E-02 2.544740E+00 3.733984E-01 1297 66 3.283750E-02 2.592400E+00 3.626000E-01 1297 67 3.325730E-02 2.862140E+00 3.730886E-01 1297 68 3.367720E-02 2.146200E+00 3.584710E-01 1297 69 3.409700E-02 2.597320E+00 3.465352E-01 1297 70 3.451680E-02 2.581100E+00 3.840774E-01 1297 71 3.493670E-02 2.742740E+00 3.537286E-01 1297 72 3.535650E-02 2.286660E+00 3.508478E-01 1297 73 3.577630E-02 2.326940E+00 3.424552E-01 1297 74 3.619620E-02 2.098840E+00 3.489516E-01 1297 75 3.661600E-02 2.401900E+00 3.491462E-01 1297 76 3.703580E-02 2.430720E+00 3.369366E-01 1297 77 3.745560E-02 2.175360E+00 3.310772E-01 1297 78 3.787550E-02 2.212160E+00 3.320748E-01 1297 79 3.829530E-02 2.123620E+00 3.199440E-01 1297 80 3.871510E-02 1.645940E+00 3.149610E-01 1297 81 3.913500E-02 1.713860E+00 3.139532E-01 1297 82 3.955480E-02 1.961100E+00 3.163746E-01 1297 83 3.997460E-02 1.768020E+00 3.025264E-01 1297 84 4.039450E-02 1.741060E+00 3.076954E-01 1297 85 4.081430E-02 1.443840E+00 3.015330E-01 1297 86 4.123410E-02 1.788180E+00 3.101008E-01 1297 87 4.165390E-02 1.760760E+00 3.099230E-01 1297 88 4.207380E-02 1.385880E+00 2.883188E-01 1297 89 4.249360E-02 1.476680E+00 2.913790E-01 1297 90 4.291340E-02 1.390700E+00 2.876072E-01 1297 91 4.333330E-02 1.543480E+00 2.835438E-01 1297 92 4.375310E-02 1.463380E+00 2.925848E-01 1297 93 4.417290E-02 1.533120E+00 2.730226E-01 1297 94 4.459280E-02 1.445440E+00 2.649304E-01 1297 95 4.501260E-02 1.276060E+00 2.663462E-01 1297 96 4.543240E-02 1.485000E+00 2.735550E-01 1297 97 4.585220E-02 1.397260E+00 2.754356E-01 1297 98 4.627210E-02 1.506760E+00 2.650936E-01 1297 99 4.669190E-02 1.452340E+00 2.698636E-01 1297 100 4.711170E-02 1.286120E+00 2.692212E-01 1297 101 4.753160E-02 1.419880E+00 2.691278E-01 1297 102 4.795140E-02 1.283420E+00 2.634230E-01 1297 103 4.837120E-02 1.255220E+00 2.554516E-01 1297 104 4.879110E-02 1.166760E+00 2.565318E-01 1297 105 4.921090E-02 1.270580E+00 2.526370E-01 1297 106 4.963070E-02 9.795400E-01 2.574200E-01 1297 107 5.005050E-02 1.142560E+00 2.572080E-01 1297 108 5.047040E-02 1.129840E+00 2.526966E-01 1297 109 5.089020E-02 1.189100E+00 2.545626E-01 1297 110 5.131000E-02 7.815198E-01 2.446910E-01 1297 111 5.172990E-02 9.974802E-01 2.544476E-01 1297 112 5.214970E-02 1.155400E+00 2.491934E-01 1297 113 5.256950E-02 8.568796E-01 2.473734E-01 1297 114 5.298940E-02 9.813402E-01 2.492740E-01 1297 115 5.340920E-02 9.451400E-01 2.418608E-01 1297 116 5.382900E-02 7.164396E-01 2.466442E-01 1297 117 5.424890E-02 1.086040E+00 2.428508E-01 1297 118 5.466870E-02 1.042100E+00 2.397790E-01 1297 119 5.508850E-02 1.103640E+00 2.403748E-01 1297 120 5.550830E-02 8.133002E-01 2.396822E-01 1297 121 5.592820E-02 1.124160E+00 2.386102E-01 1297 122 5.634800E-02 8.216798E-01 2.341982E-01 1297 123 5.676780E-02 8.434800E-01 2.430224E-01 1297 124 5.718770E-02 7.657194E-01 2.350864E-01 1297 125 5.760750E-02 9.608198E-01 2.344526E-01 1297 126 5.802730E-02 7.659594E-01 2.279324E-01 1297 127 5.844720E-02 8.082998E-01 2.290926E-01 1297 128 5.886700E-02 8.931004E-01 2.314744E-01 1297 129 5.928680E-02 7.474600E-01 2.261354E-01 1297 130 5.970660E-02 8.061198E-01 2.349136E-01 1297 131 6.012650E-02 1.002240E+00 2.277452E-01 1297 132 6.054630E-02 8.494200E-01 2.237754E-01 1297 133 6.096610E-02 7.400800E-01 2.265420E-01 1297 134 6.138600E-02 7.446402E-01 2.196290E-01 1297 135 6.180580E-02 7.960000E-01 2.298130E-01 1297 136 6.222560E-02 7.070200E-01 2.220854E-01 1297 137 6.264550E-02 5.614200E-01 2.193500E-01 1297 138 6.306530E-02 7.839600E-01 2.191752E-01 1297 139 6.348510E-02 7.386000E-01 2.159734E-01 1297 140 6.390490E-02 7.089402E-01 2.213408E-01 1297 141 6.432480E-02 6.809998E-01 2.175410E-01 1297 142 6.474460E-02 6.645000E-01 2.185870E-01 1297 143 6.516440E-02 5.463000E-01 2.122380E-01 1297 144 6.558430E-02 7.715200E-01 2.181536E-01 1297 145 6.600410E-02 6.948802E-01 2.146404E-01 1297 146 6.642390E-02 5.658804E-01 2.097292E-01 1297 147 6.684380E-02 6.273804E-01 2.142528E-01 1297 148 6.726360E-02 4.950602E-01 2.072874E-01 1297 149 6.768340E-02 5.534801E-01 2.095220E-01 1297 150 6.810320E-02 6.871800E-01 2.087168E-01 1297 151 6.852310E-02 6.172202E-01 2.067830E-01 1297 152 6.894290E-02 5.545000E-01 2.106274E-01 1297 153 6.936270E-02 4.611200E-01 2.063846E-01 1297 154 6.978260E-02 6.177200E-01 2.089168E-01 1297 155 7.020240E-02 7.462596E-01 2.098170E-01 1297 156 7.062220E-02 3.936600E-01 2.066300E-01 1297 157 7.104210E-02 5.072802E-01 2.026612E-01 1297 158 7.146190E-02 4.661396E-01 2.032510E-01 1297 159 7.188170E-02 5.542598E-01 2.054160E-01 1297 160 7.230160E-02 5.457000E-01 1.990826E-01 1297 161 7.272140E-02 6.308806E-01 2.036560E-01 1297 162 7.314120E-02 6.084192E-01 2.026616E-01 1297 163 7.356100E-02 7.482004E-01 1.966678E-01 1297 164 7.398090E-02 5.025004E-01 2.032338E-01 1297 165 7.440070E-02 5.997800E-01 1.963146E-01 1297 166 7.482050E-02 5.492202E-01 2.006790E-01 1297 167 7.524040E-02 4.565000E-01 1.957610E-01 1297 168 7.566020E-02 4.888804E-01 1.905014E-01 1297 169 7.608000E-02 4.398000E-01 1.980104E-01 1297 170 7.649980E-02 6.183398E-01 1.950386E-01 1297 171 7.691970E-02 3.787597E-01 1.947872E-01 1297 172 7.733950E-02 5.351600E-01 1.994400E-01 1297 173 7.775930E-02 4.159392E-01 1.945258E-01 1297 174 7.817920E-02 3.963600E-01 1.964648E-01 1297 175 7.859900E-02 6.001202E-01 1.939740E-01 1297 176 7.901880E-02 4.418396E-01 1.956420E-01 1297 177 7.943870E-02 5.065400E-01 1.916268E-01 1297 178 7.985850E-02 4.593606E-01 1.946470E-01 1297 179 8.027830E-02 4.747602E-01 1.965986E-01 1297 180 8.069810E-02 5.651800E-01 1.888094E-01 1297 181 8.111800E-02 5.277200E-01 1.931382E-01 1297 182 8.153780E-02 4.937802E-01 1.892820E-01 1297 183 8.195760E-02 4.319998E-01 1.917210E-01 1297 184 8.237750E-02 3.683804E-01 1.903206E-01 1297 185 8.279730E-02 3.749394E-01 1.888600E-01 1297 186 8.321710E-02 4.791600E-01 1.943992E-01 1297 187 8.363700E-02 5.860400E-01 1.974094E-01 1297 188 8.405680E-02 5.986400E-01 1.977646E-01 1297 189 8.447660E-02 5.465202E-01 2.006478E-01 1297 190 8.489650E-02 5.395400E-01 2.005206E-01 1297 191 8.531630E-02 4.642404E-01 2.071710E-01 1297 192 8.573610E-02 4.074402E-01 1.994562E-01 1297 193 8.615590E-02 3.380398E-01 2.016142E-01 1297 194 8.657580E-02 3.018006E-01 2.011640E-01 1297 195 8.699560E-02 6.019206E-01 2.047774E-01 1297 196 8.741540E-02 5.308798E-01 2.049906E-01 1297 197 8.783530E-02 3.829398E-01 2.057988E-01 1297 198 8.825510E-02 5.787996E-01 2.083574E-01 1297 199 8.867490E-02 4.717200E-01 2.120642E-01 1297 200 8.909480E-02 5.846592E-01 2.091048E-01 1297 201 8.951460E-02 3.503596E-01 2.072956E-01 1297 202 8.993440E-02 3.816602E-01 2.041960E-01 1297 203 9.035420E-02 3.550995E-01 2.080688E-01 1297 204 9.077410E-02 3.469800E-01 2.055414E-01 1297 205 9.119390E-02 3.952404E-01 2.092866E-01 1297 206 9.161370E-02 3.172807E-01 2.062158E-01 1297 207 9.203360E-02 3.141198E-01 2.091204E-01 1297 208 9.245340E-02 4.851208E-01 2.068232E-01 1297 209 9.287320E-02 3.976801E-01 2.061610E-01 1297 210 9.329310E-02 4.295404E-01 2.126808E-01 1297 211 9.371290E-02 2.032202E-01 2.133368E-01 1297 212 9.413270E-02 2.122400E-01 2.108902E-01 1297 213 9.455250E-02 3.365202E-01 2.082614E-01 1297 214 9.497240E-02 3.701794E-01 2.090098E-01 1297 215 9.539220E-02 3.598002E-01 2.071846E-01 1297 216 9.581200E-02 3.438602E-01 2.016716E-01 1297 217 9.623190E-02 3.649000E-01 2.085938E-01 1297 218 9.665170E-02 2.988194E-01 2.056762E-01 1297 219 9.707150E-02 3.061200E-01 2.057720E-01 1297 220 9.749140E-02 4.334800E-01 2.019954E-01 1297 221 9.791120E-02 4.246403E-01 2.016792E-01 1297 222 9.833100E-02 4.879596E-01 2.034294E-01 1297 223 9.875080E-02 2.634994E-01 1.976284E-01 1297 224 9.917070E-02 3.751398E-01 2.011076E-01 1297 225 9.959050E-02 3.483006E-01 2.037696E-01 1297 226 1.000100E-01 2.898208E-01 1.958694E-01 1297 227 1.004300E-01 4.008400E-01 2.010528E-01 1297 228 1.008500E-01 3.310604E-01 1.944922E-01 1297 229 1.012700E-01 4.110804E-01 2.000182E-01 1297 230 1.016900E-01 2.922997E-01 1.948804E-01 1297 231 1.021090E-01 3.680800E-01 1.928632E-01 1297 232 1.025290E-01 3.592794E-01 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