{ "code": "SASDAJ8", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.", "publication": { "title": "Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum.", "author_list": "Banerjee A, Herman E, Kottke T, Essen LO", "journal": "Structure", "doi": "10.1016/j.str.2015.10.022", "pmid": "26688213", "published_date": "2016 Jan 5" }, "status": "released", "submitted_date": "2015-06-18", "released_date": "2016-01-05" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDAJ8.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAJ8.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAJ8_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAJ8_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAJ8_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAJ8_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDAJ8.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Aureochrome1a-A´α-LOV-Jα", "short_name": "Aureochrome1a-A", "sequence": "MGSSHHHHHHSSGLVPRGSHMDFSFIKALQTAQQNFVVTDPSLPDNPIVYASQGFLNLTGYSLDQILGRNCRFLQGPETDPKAVERIRKAIEQGNDMSVCLLNYRVDGTTFWNQFFIAALRDAGGNVTNFVGVQCKVSDQYAATVTKQQEEEEEAAANDDED\r\n", "organism": "Phaeodactylum tricornutum", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 17.973, "total_mw": 35.946, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "10 mM Tris 300 mM NaCl", "concentration_unit": "mM", "comment": null, "additive": "300 mM NaCl", "concentration": 10.0, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Light state solution structure of Aureochrome1a- A´α-LOV-Jα", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Marburg, Germany", "full_name": "Philipps-Universität Marburg", "webpage": "" } ], "contributor_name": "Ankan", "contributor_surname": "Banerjee", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2015-03-16", "storage_temperature": 4.0, "cell_temperature": null, "exposure_time": 20.0, "number_of_frames": 10, "wavelength": 0.93, "sample_detector_distance": 2.43, "concentration_min": 0.75, "concentration_max": 15.0, "sample_volume": null, "flow_rate": null, "s_min": 0.034, "s_max": 4.975, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAJ8_fit1_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAJ8_fit1_model1.pdb", "model_mw": 60.432, "bead_radius": 3.25, "log": null, "symmetry": "", "comment": "The model is an averaged and volume corrected representation (DAMFILT)", "user": 72 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAJ8_fit1_fit_img.png", "software": null, "chi_square_value": 0.656, "p_value": 5e-06, "fit_residual_plot": "SASDAJ8_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDAJ8_fit1.fir", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": "Solution scattering data of Aureochrome1a- A´α-LOV-Jα in the light state (blue light illuminated for 5-10 min). The measurement was performed at two concentration 42 and 833 micomolar concentration. The buffer-only scattering was subtracted and the data sets were merged to generate representative curve. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.", "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 63.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 63.8, "pddf_i0_error": null, "guinier_i0": 62.75, "guinier_i0_error": null, "pddf_rg": 3.51, "pddf_rg_error": null, "guinier_rg": 3.34, "guinier_rg_error": null, "pddf_dmax": 13.0, "pddf_dmax_error": null, "porod_volume": 87.66, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 36, "guinier_point_last": 65, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDAJ8_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }