data_SASDFK2_MAIN # _sas_result.id 1298 _sas_result.SASBDB_code SASDFK2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 25.1600000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 9.940 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 7 _sas_result.Guinier_point_max 18 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 265-269 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269)" _sas_scan.detc_id 15 _sas_scan.id 1298 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1259 _sas_scan.result_id 1298 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1259 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 265-269)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1259 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 1.806440E+01 1.494184E+01 1298 2 5.968380E-03 1.833640E+01 7.904294E+00 1298 3 6.388220E-03 1.759860E+01 5.999846E+00 1298 4 6.808050E-03 2.238680E+01 4.629708E+00 1298 5 7.227880E-03 2.195980E+01 3.838386E+00 1298 6 7.647710E-03 2.299180E+01 3.353796E+00 1298 7 8.067540E-03 2.080540E+01 2.895304E+00 1298 8 8.487370E-03 2.072640E+01 2.539400E+00 1298 9 8.907200E-03 1.954672E+01 2.161104E+00 1298 10 9.327030E-03 1.800322E+01 1.949212E+00 1298 11 9.746860E-03 1.855568E+01 1.778938E+00 1298 12 1.016670E-02 1.732310E+01 1.716458E+00 1298 13 1.058650E-02 1.720008E+01 1.463980E+00 1298 14 1.100630E-02 1.691826E+01 1.515408E+00 1298 15 1.142620E-02 1.691280E+01 1.365844E+00 1298 16 1.184600E-02 1.534434E+01 1.272002E+00 1298 17 1.226580E-02 1.523776E+01 1.249600E+00 1298 18 1.268570E-02 1.504106E+01 1.080295E+00 1298 19 1.310550E-02 1.435066E+01 1.151988E+00 1298 20 1.352530E-02 1.345948E+01 1.083094E+00 1298 21 1.394520E-02 1.265754E+01 9.499476E-01 1298 22 1.436500E-02 1.170616E+01 9.595590E-01 1298 23 1.478480E-02 1.232228E+01 8.632894E-01 1298 24 1.520460E-02 1.142440E+01 8.667598E-01 1298 25 1.562450E-02 1.170282E+01 7.862504E-01 1298 26 1.604430E-02 1.073664E+01 7.444488E-01 1298 27 1.646410E-02 1.051900E+01 7.252360E-01 1298 28 1.688400E-02 9.159060E+00 6.932074E-01 1298 29 1.730380E-02 9.466880E+00 7.164982E-01 1298 30 1.772360E-02 9.299040E+00 6.369054E-01 1298 31 1.814350E-02 9.295940E+00 6.421636E-01 1298 32 1.856330E-02 8.674440E+00 5.933040E-01 1298 33 1.898310E-02 8.440360E+00 5.929966E-01 1298 34 1.940290E-02 8.214840E+00 5.798102E-01 1298 35 1.982280E-02 7.968740E+00 5.488152E-01 1298 36 2.024260E-02 7.988580E+00 5.658400E-01 1298 37 2.066240E-02 7.130100E+00 5.438270E-01 1298 38 2.108230E-02 7.067200E+00 5.540746E-01 1298 39 2.150210E-02 6.567280E+00 5.128526E-01 1298 40 2.192190E-02 6.624160E+00 5.074210E-01 1298 41 2.234180E-02 6.157180E+00 4.856470E-01 1298 42 2.276160E-02 6.318320E+00 4.717688E-01 1298 43 2.318140E-02 5.421220E+00 4.553432E-01 1298 44 2.360130E-02 5.841800E+00 4.781874E-01 1298 45 2.402110E-02 4.970820E+00 4.453132E-01 1298 46 2.444090E-02 5.494120E+00 4.624188E-01 1298 47 2.486070E-02 4.953680E+00 4.370604E-01 1298 48 2.528060E-02 4.884240E+00 4.277370E-01 1298 49 2.570040E-02 4.614560E+00 4.173906E-01 1298 50 2.612020E-02 4.692440E+00 4.233334E-01 1298 51 2.654010E-02 4.514900E+00 4.176190E-01 1298 52 2.695990E-02 4.085200E+00 3.988388E-01 1298 53 2.737970E-02 4.187600E+00 4.018758E-01 1298 54 2.779960E-02 3.983120E+00 3.862662E-01 1298 55 2.821940E-02 3.918480E+00 3.876192E-01 1298 56 2.863920E-02 3.399340E+00 3.747074E-01 1298 57 2.905900E-02 4.006100E+00 3.903096E-01 1298 58 2.947890E-02 3.411440E+00 3.813876E-01 1298 59 2.989870E-02 3.152240E+00 3.760192E-01 1298 60 3.031850E-02 3.362660E+00 3.841796E-01 1298 61 3.073840E-02 3.136600E+00 3.762794E-01 1298 62 3.115820E-02 3.100680E+00 3.746554E-01 1298 63 3.157800E-02 3.235140E+00 3.757992E-01 1298 64 3.199790E-02 3.139040E+00 3.687122E-01 1298 65 3.241770E-02 2.694680E+00 3.787018E-01 1298 66 3.283750E-02 2.825960E+00 3.597496E-01 1298 67 3.325730E-02 2.619180E+00 3.558344E-01 1298 68 3.367720E-02 2.526960E+00 3.639728E-01 1298 69 3.409700E-02 2.437980E+00 3.545906E-01 1298 70 3.451680E-02 2.181600E+00 3.640304E-01 1298 71 3.493670E-02 2.294080E+00 3.433306E-01 1298 72 3.535650E-02 2.385020E+00 3.603644E-01 1298 73 3.577630E-02 2.325900E+00 3.517248E-01 1298 74 3.619620E-02 1.847980E+00 3.443140E-01 1298 75 3.661600E-02 2.336780E+00 3.509296E-01 1298 76 3.703580E-02 1.878360E+00 3.281950E-01 1298 77 3.745560E-02 1.997080E+00 3.261384E-01 1298 78 3.787550E-02 1.848180E+00 3.212014E-01 1298 79 3.829530E-02 1.892980E+00 3.212722E-01 1298 80 3.871510E-02 1.676600E+00 3.290274E-01 1298 81 3.913500E-02 1.985320E+00 3.259818E-01 1298 82 3.955480E-02 1.985100E+00 3.221984E-01 1298 83 3.997460E-02 2.224600E+00 3.135726E-01 1298 84 4.039450E-02 1.561960E+00 3.016710E-01 1298 85 4.081430E-02 1.551540E+00 3.081824E-01 1298 86 4.123410E-02 1.520540E+00 2.972254E-01 1298 87 4.165390E-02 1.812640E+00 3.061436E-01 1298 88 4.207380E-02 1.578080E+00 2.893030E-01 1298 89 4.249360E-02 1.428960E+00 2.875666E-01 1298 90 4.291340E-02 1.543820E+00 2.989304E-01 1298 91 4.333330E-02 1.142320E+00 2.838234E-01 1298 92 4.375310E-02 1.603140E+00 2.825388E-01 1298 93 4.417290E-02 1.561160E+00 2.760198E-01 1298 94 4.459280E-02 1.618060E+00 2.790884E-01 1298 95 4.501260E-02 1.647880E+00 2.711254E-01 1298 96 4.543240E-02 1.500080E+00 2.774476E-01 1298 97 4.585220E-02 1.392260E+00 2.778972E-01 1298 98 4.627210E-02 1.389940E+00 2.671336E-01 1298 99 4.669190E-02 1.494240E+00 2.722546E-01 1298 100 4.711170E-02 1.454200E+00 2.738270E-01 1298 101 4.753160E-02 1.189440E+00 2.592560E-01 1298 102 4.795140E-02 1.209600E+00 2.629100E-01 1298 103 4.837120E-02 1.070460E+00 2.533994E-01 1298 104 4.879110E-02 1.144460E+00 2.593072E-01 1298 105 4.921090E-02 1.217200E+00 2.582298E-01 1298 106 4.963070E-02 9.826800E-01 2.540894E-01 1298 107 5.005050E-02 1.345960E+00 2.566480E-01 1298 108 5.047040E-02 9.609198E-01 2.521914E-01 1298 109 5.089020E-02 1.014980E+00 2.515470E-01 1298 110 5.131000E-02 1.051220E+00 2.525302E-01 1298 111 5.172990E-02 9.819802E-01 2.510352E-01 1298 112 5.214970E-02 1.224180E+00 2.456882E-01 1298 113 5.256950E-02 9.359196E-01 2.517120E-01 1298 114 5.298940E-02 1.089340E+00 2.516650E-01 1298 115 5.340920E-02 1.106780E+00 2.472886E-01 1298 116 5.382900E-02 1.034940E+00 2.473812E-01 1298 117 5.424890E-02 9.539794E-01 2.407346E-01 1298 118 5.466870E-02 1.069720E+00 2.422320E-01 1298 119 5.508850E-02 8.274400E-01 2.386506E-01 1298 120 5.550830E-02 8.179800E-01 2.361798E-01 1298 121 5.592820E-02 7.404602E-01 2.388842E-01 1298 122 5.634800E-02 8.903996E-01 2.349350E-01 1298 123 5.676780E-02 8.968800E-01 2.396638E-01 1298 124 5.718770E-02 9.570200E-01 2.318146E-01 1298 125 5.760750E-02 8.007000E-01 2.351040E-01 1298 126 5.802730E-02 7.564200E-01 2.297554E-01 1298 127 5.844720E-02 8.731398E-01 2.253778E-01 1298 128 5.886700E-02 9.060800E-01 2.318006E-01 1298 129 5.928680E-02 6.745604E-01 2.250086E-01 1298 130 5.970660E-02 8.910396E-01 2.290202E-01 1298 131 6.012650E-02 8.397604E-01 2.223476E-01 1298 132 6.054630E-02 5.502200E-01 2.194872E-01 1298 133 6.096610E-02 7.947198E-01 2.285232E-01 1298 134 6.138600E-02 8.585402E-01 2.208674E-01 1298 135 6.180580E-02 7.715800E-01 2.227050E-01 1298 136 6.222560E-02 8.192200E-01 2.176462E-01 1298 137 6.264550E-02 7.862198E-01 2.192334E-01 1298 138 6.306530E-02 8.338404E-01 2.187088E-01 1298 139 6.348510E-02 6.772400E-01 2.183438E-01 1298 140 6.390490E-02 6.523806E-01 2.201636E-01 1298 141 6.432480E-02 6.977600E-01 2.156914E-01 1298 142 6.474460E-02 7.467400E-01 2.169292E-01 1298 143 6.516440E-02 5.764804E-01 2.146038E-01 1298 144 6.558430E-02 6.231204E-01 2.202500E-01 1298 145 6.600410E-02 5.831404E-01 2.124574E-01 1298 146 6.642390E-02 6.846404E-01 2.101498E-01 1298 147 6.684380E-02 5.661402E-01 2.109876E-01 1298 148 6.726360E-02 6.129400E-01 2.108404E-01 1298 149 6.768340E-02 5.785400E-01 2.106122E-01 1298 150 6.810320E-02 4.483198E-01 2.044062E-01 1298 151 6.852310E-02 5.749800E-01 2.135142E-01 1298 152 6.894290E-02 6.382600E-01 2.070210E-01 1298 153 6.936270E-02 7.261000E-01 2.078956E-01 1298 154 6.978260E-02 7.697000E-01 2.137180E-01 1298 155 7.020240E-02 6.946998E-01 2.066198E-01 1298 156 7.062220E-02 6.517200E-01 2.034614E-01 1298 157 7.104210E-02 5.903400E-01 2.043436E-01 1298 158 7.146190E-02 4.221592E-01 1.997080E-01 1298 159 7.188170E-02 6.077196E-01 2.044792E-01 1298 160 7.230160E-02 6.545800E-01 1.995064E-01 1298 161 7.272140E-02 3.934206E-01 2.009644E-01 1298 162 7.314120E-02 5.190996E-01 2.001064E-01 1298 163 7.356100E-02 6.229408E-01 2.016494E-01 1298 164 7.398090E-02 5.831802E-01 2.004154E-01 1298 165 7.440070E-02 4.567398E-01 1.949796E-01 1298 166 7.482050E-02 5.866000E-01 1.998378E-01 1298 167 7.524040E-02 5.395202E-01 1.962848E-01 1298 168 7.566020E-02 5.621406E-01 1.940644E-01 1298 169 7.608000E-02 4.732400E-01 1.956666E-01 1298 170 7.649980E-02 5.920398E-01 1.961764E-01 1298 171 7.691970E-02 3.831991E-01 1.956238E-01 1298 172 7.733950E-02 4.416000E-01 1.926290E-01 1298 173 7.775930E-02 5.504798E-01 1.985638E-01 1298 174 7.817920E-02 4.518600E-01 1.920390E-01 1298 175 7.859900E-02 4.343802E-01 1.919456E-01 1298 176 7.901880E-02 4.224398E-01 1.989754E-01 1298 177 7.943870E-02 3.964600E-01 1.867924E-01 1298 178 7.985850E-02 4.352204E-01 1.952730E-01 1298 179 8.027830E-02 4.959404E-01 1.942442E-01 1298 180 8.069810E-02 6.502400E-01 1.936342E-01 1298 181 8.111800E-02 4.492798E-01 1.915604E-01 1298 182 8.153780E-02 5.421006E-01 1.886858E-01 1298 183 8.195760E-02 5.178796E-01 1.960278E-01 1298 184 8.237750E-02 6.408202E-01 1.929810E-01 1298 185 8.279730E-02 3.754998E-01 1.937574E-01 1298 186 8.321710E-02 3.727198E-01 1.915484E-01 1298 187 8.363700E-02 4.100400E-01 1.951370E-01 1298 188 8.405680E-02 2.658204E-01 1.957128E-01 1298 189 8.447660E-02 3.692204E-01 2.016042E-01 1298 190 8.489650E-02 3.812404E-01 2.034526E-01 1298 191 8.531630E-02 4.582000E-01 2.062382E-01 1298 192 8.573610E-02 2.288004E-01 2.036808E-01 1298 193 8.615590E-02 4.056600E-01 2.026710E-01 1298 194 8.657580E-02 4.500204E-01 2.064566E-01 1298 195 8.699560E-02 4.961200E-01 2.047894E-01 1298 196 8.741540E-02 4.113996E-01 2.067156E-01 1298 197 8.783530E-02 2.800597E-01 2.047090E-01 1298 198 8.825510E-02 4.344396E-01 2.054188E-01 1298 199 8.867490E-02 4.191800E-01 2.140022E-01 1298 200 8.909480E-02 3.104197E-01 2.093970E-01 1298 201 8.951460E-02 4.148192E-01 2.072538E-01 1298 202 8.993440E-02 4.083404E-01 2.077732E-01 1298 203 9.035420E-02 2.155599E-01 2.048424E-01 1298 204 9.077410E-02 2.478599E-01 2.047368E-01 1298 205 9.119390E-02 3.022004E-01 2.047696E-01 1298 206 9.161370E-02 4.448808E-01 2.065712E-01 1298 207 9.203360E-02 1.563399E-01 2.085234E-01 1298 208 9.245340E-02 3.580205E-01 2.068464E-01 1298 209 9.287320E-02 5.000002E-01 2.055438E-01 1298 210 9.329310E-02 4.949006E-01 2.131476E-01 1298 211 9.371290E-02 2.489203E-01 2.100344E-01 1298 212 9.413270E-02 4.227806E-01 2.122180E-01 1298 213 9.455250E-02 3.592802E-01 2.056954E-01 1298 214 9.497240E-02 3.541797E-01 2.131392E-01 1298 215 9.539220E-02 3.757603E-01 2.085776E-01 1298 216 9.581200E-02 4.487205E-01 2.028134E-01 1298 217 9.623190E-02 2.179601E-01 2.086824E-01 1298 218 9.665170E-02 3.568200E-01 2.023070E-01 1298 219 9.707150E-02 4.845400E-01 2.044180E-01 1298 220 9.749140E-02 3.546005E-01 2.015382E-01 1298 221 9.791120E-02 2.653405E-01 2.000070E-01 1298 222 9.833100E-02 3.466596E-01 2.048552E-01 1298 223 9.875080E-02 2.911396E-01 2.004384E-01 1298 224 9.917070E-02 3.021598E-01 1.977180E-01 1298 225 9.959050E-02 2.907406E-01 1.983786E-01 1298 226 1.000100E-01 3.803005E-01 1.927058E-01 1298 227 1.004300E-01 3.390399E-01 1.948100E-01 1298 228 1.008500E-01 3.087200E-01 1.941726E-01 1298 229 1.012700E-01 1.975202E-01 2.017376E-01 1298 230 1.016900E-01 2.767398E-01 1.964404E-01 1298 231 1.021090E-01 2.009000E-01 1.917022E-01 1298 232 1.025290E-01 3.208796E-01 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