data_SASDFL2_MAIN # _sas_result.id 1299 _sas_result.SASBDB_code SASDFL2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 20.0400000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 9.009 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 6 _sas_result.Guinier_point_max 21 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 270-274 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)" _sas_scan.detc_id 15 _sas_scan.id 1299 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1260 _sas_scan.result_id 1299 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1260 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 270-274)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1260 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 5.676201E+00 1.031984E+01 1299 2 5.968380E-03 1.381520E+01 8.320836E+00 1299 3 6.388220E-03 1.872700E+01 5.617992E+00 1299 4 6.808050E-03 1.840640E+01 4.380624E+00 1299 5 7.227880E-03 1.970280E+01 3.209554E+00 1299 6 7.647710E-03 1.805920E+01 3.330962E+00 1299 7 8.067540E-03 1.685820E+01 2.749062E+00 1299 8 8.487370E-03 1.645262E+01 2.356942E+00 1299 9 8.907200E-03 1.660068E+01 2.129296E+00 1299 10 9.327030E-03 1.553938E+01 1.988806E+00 1299 11 9.746860E-03 1.584036E+01 1.815248E+00 1299 12 1.016670E-02 1.438626E+01 1.671314E+00 1299 13 1.058650E-02 1.450874E+01 1.569778E+00 1299 14 1.100630E-02 1.468678E+01 1.455878E+00 1299 15 1.142620E-02 1.415120E+01 1.239320E+00 1299 16 1.184600E-02 1.389420E+01 1.253794E+00 1299 17 1.226580E-02 1.301286E+01 1.185872E+00 1299 18 1.268570E-02 1.252122E+01 1.033189E+00 1299 19 1.310550E-02 1.277956E+01 1.080309E+00 1299 20 1.352530E-02 1.251250E+01 9.724156E-01 1299 21 1.394520E-02 1.183452E+01 9.668822E-01 1299 22 1.436500E-02 1.121112E+01 9.371736E-01 1299 23 1.478480E-02 1.064608E+01 8.804328E-01 1299 24 1.520460E-02 1.083286E+01 8.702824E-01 1299 25 1.562450E-02 1.018812E+01 7.827666E-01 1299 26 1.604430E-02 9.824400E+00 7.459486E-01 1299 27 1.646410E-02 9.642760E+00 7.150156E-01 1299 28 1.688400E-02 9.285680E+00 6.621798E-01 1299 29 1.730380E-02 8.837740E+00 7.038980E-01 1299 30 1.772360E-02 8.190540E+00 6.226824E-01 1299 31 1.814350E-02 8.561080E+00 6.265166E-01 1299 32 1.856330E-02 7.373360E+00 5.911686E-01 1299 33 1.898310E-02 7.908360E+00 5.762934E-01 1299 34 1.940290E-02 7.492940E+00 5.710158E-01 1299 35 1.982280E-02 7.022560E+00 5.435148E-01 1299 36 2.024260E-02 7.023800E+00 5.498576E-01 1299 37 2.066240E-02 7.013320E+00 5.135478E-01 1299 38 2.108230E-02 6.328640E+00 5.193506E-01 1299 39 2.150210E-02 6.166560E+00 5.289554E-01 1299 40 2.192190E-02 6.457440E+00 5.094434E-01 1299 41 2.234180E-02 6.025820E+00 4.886582E-01 1299 42 2.276160E-02 5.412480E+00 4.585868E-01 1299 43 2.318140E-02 5.392480E+00 4.636434E-01 1299 44 2.360130E-02 5.161920E+00 4.556758E-01 1299 45 2.402110E-02 5.096600E+00 4.488482E-01 1299 46 2.444090E-02 4.786600E+00 4.646112E-01 1299 47 2.486070E-02 4.786260E+00 4.310152E-01 1299 48 2.528060E-02 4.567000E+00 4.276976E-01 1299 49 2.570040E-02 4.173840E+00 4.161352E-01 1299 50 2.612020E-02 4.193140E+00 4.007308E-01 1299 51 2.654010E-02 3.933600E+00 4.073860E-01 1299 52 2.695990E-02 4.148680E+00 3.928592E-01 1299 53 2.737970E-02 3.893720E+00 3.997598E-01 1299 54 2.779960E-02 4.088120E+00 3.907944E-01 1299 55 2.821940E-02 3.652180E+00 3.865760E-01 1299 56 2.863920E-02 3.410720E+00 3.849744E-01 1299 57 2.905900E-02 3.345580E+00 3.907392E-01 1299 58 2.947890E-02 3.481580E+00 3.952254E-01 1299 59 2.989870E-02 3.156080E+00 3.671084E-01 1299 60 3.031850E-02 3.391100E+00 3.838544E-01 1299 61 3.073840E-02 2.921240E+00 3.719114E-01 1299 62 3.115820E-02 2.654940E+00 3.764976E-01 1299 63 3.157800E-02 2.906580E+00 3.786620E-01 1299 64 3.199790E-02 2.762040E+00 3.551344E-01 1299 65 3.241770E-02 2.573860E+00 3.770116E-01 1299 66 3.283750E-02 3.060080E+00 3.681480E-01 1299 67 3.325730E-02 3.020480E+00 3.615194E-01 1299 68 3.367720E-02 2.655340E+00 3.645292E-01 1299 69 3.409700E-02 2.513560E+00 3.380736E-01 1299 70 3.451680E-02 2.401280E+00 3.612998E-01 1299 71 3.493670E-02 2.593640E+00 3.469900E-01 1299 72 3.535650E-02 2.435480E+00 3.422158E-01 1299 73 3.577630E-02 2.218160E+00 3.423636E-01 1299 74 3.619620E-02 2.205880E+00 3.451148E-01 1299 75 3.661600E-02 2.348660E+00 3.450416E-01 1299 76 3.703580E-02 2.143420E+00 3.321270E-01 1299 77 3.745560E-02 2.066260E+00 3.250078E-01 1299 78 3.787550E-02 2.235780E+00 3.220962E-01 1299 79 3.829530E-02 1.762860E+00 3.210068E-01 1299 80 3.871510E-02 2.021020E+00 3.265364E-01 1299 81 3.913500E-02 1.775540E+00 3.189340E-01 1299 82 3.955480E-02 1.994280E+00 3.110336E-01 1299 83 3.997460E-02 1.710700E+00 3.071388E-01 1299 84 4.039450E-02 1.984060E+00 3.080286E-01 1299 85 4.081430E-02 1.656960E+00 2.986328E-01 1299 86 4.123410E-02 1.573040E+00 2.947094E-01 1299 87 4.165390E-02 1.716980E+00 3.023774E-01 1299 88 4.207380E-02 1.633040E+00 2.922056E-01 1299 89 4.249360E-02 1.407080E+00 2.950732E-01 1299 90 4.291340E-02 1.522100E+00 2.907050E-01 1299 91 4.333330E-02 1.379500E+00 2.897002E-01 1299 92 4.375310E-02 1.686120E+00 2.897852E-01 1299 93 4.417290E-02 1.235440E+00 2.735866E-01 1299 94 4.459280E-02 1.400200E+00 2.779284E-01 1299 95 4.501260E-02 1.484300E+00 2.753368E-01 1299 96 4.543240E-02 1.115060E+00 2.706642E-01 1299 97 4.585220E-02 1.338880E+00 2.759138E-01 1299 98 4.627210E-02 1.226520E+00 2.620906E-01 1299 99 4.669190E-02 1.412340E+00 2.718122E-01 1299 100 4.711170E-02 1.541080E+00 2.644888E-01 1299 101 4.753160E-02 1.250580E+00 2.641346E-01 1299 102 4.795140E-02 1.149940E+00 2.692100E-01 1299 103 4.837120E-02 1.303580E+00 2.584652E-01 1299 104 4.879110E-02 1.355280E+00 2.666854E-01 1299 105 4.921090E-02 1.165240E+00 2.560442E-01 1299 106 4.963070E-02 1.005000E+00 2.550854E-01 1299 107 5.005050E-02 1.004340E+00 2.512508E-01 1299 108 5.047040E-02 8.771798E-01 2.501450E-01 1299 109 5.089020E-02 1.064220E+00 2.493744E-01 1299 110 5.131000E-02 1.066820E+00 2.462496E-01 1299 111 5.172990E-02 9.810600E-01 2.527878E-01 1299 112 5.214970E-02 8.488798E-01 2.407384E-01 1299 113 5.256950E-02 9.984998E-01 2.513982E-01 1299 114 5.298940E-02 1.093120E+00 2.501098E-01 1299 115 5.340920E-02 9.772400E-01 2.480122E-01 1299 116 5.382900E-02 6.291394E-01 2.416540E-01 1299 117 5.424890E-02 8.218398E-01 2.358620E-01 1299 118 5.466870E-02 6.942200E-01 2.419890E-01 1299 119 5.508850E-02 9.390800E-01 2.406548E-01 1299 120 5.550830E-02 8.868600E-01 2.403766E-01 1299 121 5.592820E-02 9.268200E-01 2.380322E-01 1299 122 5.634800E-02 8.354800E-01 2.360228E-01 1299 123 5.676780E-02 8.541002E-01 2.408130E-01 1299 124 5.718770E-02 6.914194E-01 2.297496E-01 1299 125 5.760750E-02 8.821600E-01 2.342658E-01 1299 126 5.802730E-02 7.431000E-01 2.303718E-01 1299 127 5.844720E-02 7.784998E-01 2.333154E-01 1299 128 5.886700E-02 9.111204E-01 2.316466E-01 1299 129 5.928680E-02 7.928000E-01 2.231416E-01 1299 130 5.970660E-02 8.453796E-01 2.291028E-01 1299 131 6.012650E-02 6.790006E-01 2.234522E-01 1299 132 6.054630E-02 6.230400E-01 2.243150E-01 1299 133 6.096610E-02 7.678600E-01 2.252798E-01 1299 134 6.138600E-02 1.054140E+00 2.237176E-01 1299 135 6.180580E-02 4.642600E-01 2.226068E-01 1299 136 6.222560E-02 8.199600E-01 2.203260E-01 1299 137 6.264550E-02 6.381398E-01 2.153486E-01 1299 138 6.306530E-02 6.160200E-01 2.192630E-01 1299 139 6.348510E-02 7.656600E-01 2.149420E-01 1299 140 6.390490E-02 7.939400E-01 2.177314E-01 1299 141 6.432480E-02 7.883598E-01 2.160784E-01 1299 142 6.474460E-02 6.547398E-01 2.172742E-01 1299 143 6.516440E-02 5.662400E-01 2.120368E-01 1299 144 6.558430E-02 5.855004E-01 2.158610E-01 1299 145 6.600410E-02 6.466600E-01 2.122552E-01 1299 146 6.642390E-02 5.844406E-01 2.101042E-01 1299 147 6.684380E-02 5.473002E-01 2.104004E-01 1299 148 6.726360E-02 5.133801E-01 2.124416E-01 1299 149 6.768340E-02 7.194602E-01 2.132490E-01 1299 150 6.810320E-02 4.710998E-01 2.086378E-01 1299 151 6.852310E-02 6.061402E-01 2.075744E-01 1299 152 6.894290E-02 4.805800E-01 2.076686E-01 1299 153 6.936270E-02 5.208800E-01 2.047710E-01 1299 154 6.978260E-02 6.374798E-01 2.084722E-01 1299 155 7.020240E-02 5.452996E-01 2.037320E-01 1299 156 7.062220E-02 5.750602E-01 2.049104E-01 1299 157 7.104210E-02 5.041402E-01 2.043762E-01 1299 158 7.146190E-02 5.756794E-01 1.999044E-01 1299 159 7.188170E-02 4.034992E-01 2.014458E-01 1299 160 7.230160E-02 6.268400E-01 1.998556E-01 1299 161 7.272140E-02 4.117600E-01 2.030430E-01 1299 162 7.314120E-02 5.658992E-01 1.989158E-01 1299 163 7.356100E-02 6.030802E-01 2.011116E-01 1299 164 7.398090E-02 5.416408E-01 1.999106E-01 1299 165 7.440070E-02 6.467798E-01 1.990740E-01 1299 166 7.482050E-02 6.299602E-01 1.970212E-01 1299 167 7.524040E-02 5.529000E-01 1.958320E-01 1299 168 7.566020E-02 3.546806E-01 1.946798E-01 1299 169 7.608000E-02 5.329004E-01 1.954338E-01 1299 170 7.649980E-02 5.999000E-01 1.927052E-01 1299 171 7.691970E-02 5.306196E-01 1.971032E-01 1299 172 7.733950E-02 4.580400E-01 1.932006E-01 1299 173 7.775930E-02 4.350400E-01 1.919960E-01 1299 174 7.817920E-02 5.180200E-01 1.931356E-01 1299 175 7.859900E-02 4.230001E-01 1.939898E-01 1299 176 7.901880E-02 5.624796E-01 1.964356E-01 1299 177 7.943870E-02 4.142200E-01 1.945952E-01 1299 178 7.985850E-02 4.794604E-01 1.934832E-01 1299 179 8.027830E-02 3.946400E-01 1.935370E-01 1299 180 8.069810E-02 4.548200E-01 1.914542E-01 1299 181 8.111800E-02 4.448996E-01 1.967650E-01 1299 182 8.153780E-02 4.911604E-01 1.901328E-01 1299 183 8.195760E-02 4.115596E-01 1.948760E-01 1299 184 8.237750E-02 5.712604E-01 1.933474E-01 1299 185 8.279730E-02 2.590200E-01 1.893160E-01 1299 186 8.321710E-02 3.211995E-01 1.907768E-01 1299 187 8.363700E-02 4.538800E-01 1.958396E-01 1299 188 8.405680E-02 3.717800E-01 1.988084E-01 1299 189 8.447660E-02 1.675406E-01 1.987876E-01 1299 190 8.489650E-02 4.291604E-01 2.039082E-01 1299 191 8.531630E-02 2.908201E-01 1.999014E-01 1299 192 8.573610E-02 3.620404E-01 2.040716E-01 1299 193 8.615590E-02 5.379794E-01 2.101544E-01 1299 194 8.657580E-02 2.843606E-01 1.998162E-01 1299 195 8.699560E-02 5.443802E-01 2.105868E-01 1299 196 8.741540E-02 4.933199E-01 2.071238E-01 1299 197 8.783530E-02 5.266800E-01 2.043208E-01 1299 198 8.825510E-02 2.087195E-01 2.077020E-01 1299 199 8.867490E-02 4.240200E-01 2.136124E-01 1299 200 8.909480E-02 3.857197E-01 2.082470E-01 1299 201 8.951460E-02 3.905000E-01 2.059476E-01 1299 202 8.993440E-02 4.560800E-01 2.044242E-01 1299 203 9.035420E-02 3.569598E-01 2.075008E-01 1299 204 9.077410E-02 3.271599E-01 2.028316E-01 1299 205 9.119390E-02 5.497806E-01 2.093620E-01 1299 206 9.161370E-02 4.742806E-01 2.102210E-01 1299 207 9.203360E-02 1.873798E-01 2.085016E-01 1299 208 9.245340E-02 2.607404E-01 2.082974E-01 1299 209 9.287320E-02 3.017005E-01 2.054164E-01 1299 210 9.329310E-02 4.356604E-01 2.092830E-01 1299 211 9.371290E-02 4.136200E-01 2.160224E-01 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