data_SASDBL4_MAIN # _sas_result.id 339 _sas_result.SASBDB_code SASDBL4 _sas_result.experimental_MW 225.000 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR 160.4000000000 _sas_result.I0_from_PR_error 0.0600000000 _sas_result.I0_from_Guinier 160.6000000000 _sas_result.I0_from_Guinier_error 0.1000000000 _sas_result.Rg_from_PR 5.440 _sas_result.Rg_from_PR_error 0.030 _sas_result.Rg_from_Guinier 5.430 _sas_result.Rg_from_Guinier_error 0.060 _sas_result.D_max 18.40 _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 11 _sas_result.Guinier_point_max 34 _sas_result.type_of_curve single_conc _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments . # _sas_scan.title "1:1:6:1 crRNA:Cas6f:Cas7fv:Cas5fv complex" _sas_scan.detc_id 16 _sas_scan.id 339 _sas_scan.measurement_date 2015-06-27 _sas_scan.storage_temperature 4.0 _sas_scan.cell_temperature 4.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 10 _sas_scan.unit 1/nm _sas_scan.qmin 0.0327 _sas_scan.qmax 4.9336 _sas_scan.sample_id 300 _sas_scan.result_id 339 _sas_scan.beam_id 10 # _sas_beam.id 10 _sas_beam.instrument_name "ESRF BM29" _sas_beam.instrument_city Grenoble _sas_beam.instrument_country France _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.930000 _sas_beam.dist_spec_to_detc 2.43000 # _sas_detc.id 16 _sas_detc.name "Pilatus 1M" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 300 _sas_sample.name "The 1:1:6:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F wild-type Cascade complex" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 20.00 _sas_sample.concentration_method . loop_ _sas_sample.entity_id 329 331 332 334 # _sas_buffer.id 151 _sas_buffer.name HEPES _sas_buffer.concentration 50.00 _sas_buffer.unit mM _sas_buffer.pK . _sas_buffer.pH 7.000 _sas_buffer.comment . _sas_buffer.composition "150 mM NaCl, 1mM DTT, 1mM EDTA" _sas_buffer.sample_id 300 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 329 protein "Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)" 20.674 1 331 protein "Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822)" 37.861 1 332 protein "Hexameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)" 37.168 6 334 RNA "wildtype-crRNA: CRISPR/Cas Type I-F Cascade component" 19.290 1 # loop_ _entity_name_com.entity_id _entity_name_com.name 329 Cas6f 331 Cas5fv 332 Cas7fv-hexamer 334 wildtype-crRNA # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 329 MNSYIDIRLKPDAEMREAELSSKVFTKFHKALVTLNSHKIGISFPQMKLSLGQLFRIHGDASLLHDLQGLDWLGPLAGYCQVTAVSAVPDHVQYRIVSVKRSNLSKAKLKRLIARGSIDKDGEKRYKVKMLGQGFDNPYLDLFSSSTGQVYRKFFEFSDIQAHPLDGEFDSYGLSKTATVPWF 331 MKIIIEYDSCWRNAFLGGSNNEPVPKKGREFLGSMTSLKKEGNFKVCENTLDTVMGVLNRLIGDQRKLYQARSKMYESAYYFEALEDKVSFIDKPQLTNEISFIRNMNGSTDQNAFTGMIKVSDPVFTSEYSQQFWGVLALDFTQLCDFIIKQSQVVGSIELNPLSIINRLESLNQEKALENSDDLAQVLKVLNEYFPDIEYLNNKGLITPISIYCSALYLQLARLETSFNMTTAKTKAGGISGISKRGFTKKDFMDRYTTGPKKTIWGNPFIKKEKIKGQGEVTSMMTKASGQLEISIDVDRDKAQEIKILIENAGVSSFYLGKKGLAYVSNIKL 332 MGSSHHHHHHSQNPMQKVTGIKSVDFKIKALGHGVVNWNGPTTLTGDDGKTVDNHTLPKLRGYTNLTGKVKDETGYKYKKQATDINFKETPLYISQNCIRHHLFREQAFDLHYASDKNLKNVLASITGLIRGYVVPSSQCKRTSPLLLEDFVDQLGNGNFEQYGQAGARDSTSFFSKTTFGDTEYISYGSISIEQLQFISLDKKFDRAAMVIKEGEGEVIAAELQNYIQSLNPSLNPQAIFHSNYVRRGTIFEEGECGILLNDDAVKALVAETLERLANLSIRQAKGYMYVDDITVDYNDSHKMMRIKRDESEIINEQHAPFAQYFYAK 334 CUUAGAAAUAUCGCCCAGCAAGACGCGCAAACCUAUAACCGUUCACCGCCGCACAGGCGG # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 329 "Shewanella putrefaciens" 331 "Shewanella putrefaciens" 332 "Shewanella putrefaciens" 334 . # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 329 UniProt A4Y6G3 http://www.uniprot.org/uniprot/A4Y6G3 2 331 UniProt A4Y6G2 http://www.uniprot.org/uniprot/A4Y6G2 3 332 UniProt A4Y6G1 http://www.uniprot.org/uniprot/A4Y6G1