{ "code": "SASDFL4", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Solution structure, glycan specificity and of phenol oxidase inhibitory activity of Anopheles C-type lectins CTL4 and CTLMA2.", "publication": { "title": "Solution structure, glycan specificity and of phenol oxidase inhibitory activity of Anopheles C-type lectins CTL4 and CTLMA2.", "author_list": "Bishnoi R, Sousa GL, Contet A, Day CJ, Hou CD, Profitt LA, Singla D, Jennings MP, Valentine AM, Povelones M, Baxter RHG", "journal": "Sci Rep", "doi": "10.1038/s41598-019-51353-z", "pmid": "31645596", "published_date": "2019 Oct 23" }, "status": "released", "submitted_date": "2019-03-25", "released_date": "2019-04-08" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFL4.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFL4.txt", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFL4_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFL4_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFL4_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFL4_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFL4.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 100K", "resolution": 172.0 }, "name": "Thomas Jefferson University", "city": "Philadelphia, PA", "country": "USA", "beamline_name": "Rigaku BioSAXS-2000", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "AGAP005335-PA", "short_name": "CTL4", "sequence": "YRATTAREMITQNLCVCPCGNPRGGKLYTTPNLRLN\r\nWFDAVSYCSSIGMSIATIKDTNERQLLQLHLDGDRRLTRSQKRSKIPYWIGANSLIAGQG\r\nLRWGLTDQEVKESAEWADGIAPANNRVEPFCVYIQGSTMSWVATSCDDEPRQFICEYHHHHHH", "organism": "Anopheles gambiae", "uniprot_code": "Q7Q7F7", "uniprot_range_first": 25, "uniprot_range_last": 177, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MQISNIFVSSIGITLVCLIVSTHGYRATTAREMITQNLCVCPCGNPRGGKLYTTPNLRLN\nWFDAVSYCSSIGMSIATIKDTNERQLLQLHLDGDRRLTRSQKRSKIPYWIGANSLIAGQG\nLRWGLTDQEVKESAEWADGIAPANNRVEPFCVYIQGSTMSWVATSCDDEPRQFICEY", "mw": 18.095, "total_mw": 18.095, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "AGAP005334-PA", "short_name": "CTLMA2", "sequence": "DLSVPKTVDDILQQNPCLCPCKPFEEKEYFIPISRTSDWFGAV\r\nAYCHSVGMELAEVLNEDEARAMGEVIAEEESDSDDEFYWIGANDLGVQGTYRWALAGRPV\r\nLYSQWAAGEPNHARGENGQQPAERCVAVAMDKYEWNDFQCTQQKPFICQQFRKH", "organism": "Anopheles gambiae", "uniprot_code": "Q7Q7F8", "uniprot_range_first": 18, "uniprot_range_last": 174, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MNLMITILIAVITVVRGDLSVPKTVDDILQQNPCLCPCKPFEEKEYFIPISRTSDWFGAV\nAYCHSVGMELAEVLNEDEARAMGEVIAEEESDSDDEFYWIGANDLGVQGTYRWALAGRPV\nLYSQWAAGEPNHARGENGQQPAERCVAVAMDKYEWNDFQCTQQKPFICQQFRKH", "mw": 17.874, "total_mw": 17.874, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "500 mM NaCl, 20 mM CHES, 0.5 mM CaCl2, 1% glycerol", "concentration_unit": null, "comment": "necessary to prevent formation of higher order oligomers", "additive": null, "concentration": null, "pka": null, "ph": 9.0, "deuteration": null }, "purity_method": null, "name": "C-type lectins CTL4/CTLMA2, 3.1 mg/ml", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Philadelphia, USA", "full_name": "Temple University", "webpage": "https://www.temple.edu/" } ], "contributor_name": "Richard", "contributor_surname": "Baxter", "orcid": "https://orcid.org/0000-0001-5140-1726" } ], "concentration_method": null, "concentration_unit": null, "date": "2018-08-30", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 300.0, "number_of_frames": 6, "wavelength": 0.154187, "sample_detector_distance": 0.481, "concentration_min": null, "concentration_max": 3.1, "sample_volume": null, "flow_rate": null, "s_min": 0.066, "s_max": 6.94, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFL4_fit1_model1_img.png", "software": "MultiFoXS", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFL4_fit1_model1.pdb", "model_mw": 35.95, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 424 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFL4_fit1_model2_img.png", "software": "MultiFoXS", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFL4_fit1_model2.pdb", "model_mw": 35.95, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 424 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFL4_fit1_model3_img.png", "software": "MultiFoXS", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFL4_fit1_model3.pdb", "model_mw": 35.95, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 424 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/multi_state_model_3_1_1_fitfoxs_img.png", "software": "MultiFoXS", "chi_square_value": 1.072759, "p_value": 0.481779, "fit_residual_plot": "multi_state_model_3_1_1_fitfoxsresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFL4_fit1.dat", "fit_log": null, "software_version": null, "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.6", "i0_calibration_standard": null, "description": null, "experiment_description": "SAXS data from solutions of C-type lectins CTL4/CTLMA2 in 500 mM NaCl, 20 mM CHES, 0.5 mM CaCl2, 1% glycerol, pH 9 were collected on a Rigaku BioSAXS-2000 instrument at Thomas Jefferson University (Philadelphia, PA, USA) using a Pilatus 100K detector at a sample-detector distance of 0.481 m and at a wavelength of λ = 0.154187 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.10 mg/ml was measured at 20°C. Six successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 39.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 36.0, "porod_mw_error": null, "pddf_i0": 0.6474, "pddf_i0_error": null, "guinier_i0": 0.65, "guinier_i0_error": 0.01, "pddf_rg": 2.54, "pddf_rg_error": null, "guinier_rg": 2.49, "guinier_rg_error": 0.03, "pddf_dmax": 8.0, "pddf_dmax_error": null, "porod_volume": 58.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 9, "guinier_point_last": 32, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDFL4_datloglog_img.png", "symmetry": null, "last_modified": "2025-03-18T10:29:02.995592+01:00", "bragg_peak": [] }