{ "code": "SASDFL6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.", "publication": { "title": "Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.", "author_list": "Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT", "journal": "J Mol Biol", "doi": "10.1016/j.jmb.2019.10.001", "pmid": "31628946", "published_date": "2019 Oct 16" }, "status": "released", "submitted_date": "2019-05-31", "released_date": "2019-11-17" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFL6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFL6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFL6_dat_img.png", "intensities_kratky_plot": 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"MLLKNFPQTIKDGQVSLSAIKMLLGFNESMNDISGYELTWTGKGFANALYSEPCQKQLKL\nQESFTPQTSASKHPNNAIIIGDNLDALKLLKSAYSEKIKMIYIDPPYNTGNDEFIYPDNF\nRQDYQKILREVGLMEIDENGKEIESESLKFFKNTQGSGTHSGWLSFMLPRLKLARDLLKE\nDGVIFISIDDNECANLKILCDEIFGEDNFVGDFIRKTKSTTNDAKIGLNYQHEFLLCYAK\nDKNYTNLLGGEKNLENYKNPDNDPNGAWINDNPSAKSGNMKTGYFGVTNPYTNKVDYPPV\nGMFWRFSQNTIQKHIDEGRICFKKEHKDNERGFIYKRYLKDLKTTQKTFDSLIFSDNCYM\nNQAATKELLNLGMGEYFTYPKGVEFMKKIILHSTTPNEGDIILDFFAGSGTTVHAVMELN\nAEDKGNREFILVQIDEEIKEDESAYDFCKKELKSAKPVISDITIERVKRAAQKISQLSKD\nSGLDLGFKVYTLQDKVQIINDKEEITLFNRSDLTPFDKALNLALQCGKTLNQALEIIIKD\nKLYKCEDAYFCIVCDEEAQEYLAKSKNEMIFLDGYEEIDLEAFLNLNASFKERLSVVY", "mw": 68.3, "total_mw": 136.6, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris, 250 mM NaCl", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "RCB", "address": "Haryana, India", "full_name": "Regional Centre for Biotechnology", "webpage": "http://www.rcb.res.in/" } ], "contributor_name": "Naveen", "contributor_surname": "Narayanan", "orcid": "https://orcid.org/0000-0001-8327-1559" } ], "concentration_method": null, "concentration_unit": null, "date": "2017-07-09", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 0.5, "number_of_frames": 10, "wavelength": 0.0992, "sample_detector_distance": null, "concentration_min": null, "concentration_max": 2.0, "sample_volume": null, "flow_rate": null, "s_min": 0.036, "s_max": 4.939, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFL6_fit1_model1_img.png", "software": "PyMOL", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFL6_fit1_model1.pdb", "model_mw": 117.9, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 451 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/dimer1_k366e_minim01_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.219, "p_value": 0.0, "fit_residual_plot": "dimer1_k366e_minim01_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFL6_fit1.fit", "fit_log": null, "software_version": "2.8.3", "description": "" }, { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFL6_fit2_model1_img.png", "software": "PyMOL", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFL6_fit2_model1.pdb", "model_mw": 117.9, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 451 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/dimer2_a194_minimized01_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 2.085, "p_value": 0.0, "fit_residual_plot": "dimer2_a194_minimized01_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFL6_fit2.fit", "fit_log": null, "software_version": "2.8.3", "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod Hp_0593) at pH 8 in 25 mM Tris, 250 mM NaCl were collected on the ID14-3 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 120.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 90.0, "porod_mw_error": null, "pddf_i0": 35.98, "pddf_i0_error": null, "guinier_i0": 35.57, "guinier_i0_error": 0.086, "pddf_rg": 3.375, "pddf_rg_error": null, "guinier_rg": 3.27, "guinier_rg_error": 0.74, "pddf_dmax": 12.5, "pddf_dmax_error": null, "porod_volume": 143.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 30, "guinier_point_last": 77, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDFL6_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }