{ "code": "SASDXL6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "In vitro characterization of the catalytic domain of human histone deacetylase 5", "publication": { "title": "In vitro characterization of the catalytic domain of human histone deacetylase 5", "author_list": "Mammen C, Hornung F, Anzenhofer C, Schumacher J, Reiners J, Li J, Mazzone F, Bilsing F, Kassack M, Kurz T, Smits S", "journal": "Scientific Reports", "doi": "10.1038/s41598-026-37633-5", "pmid": null, "published_date": "2026 Feb 27" }, "status": "released", "submitted_date": "2025-07-21", "released_date": "2025-11-05" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXL6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXL6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXL6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXL6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXL6.sascif", "experiment": { "instrument": { "detector": { "type": "pixel counting", "name": "Pilatus3 R 300K", "resolution": 172.0 }, "name": "Center for Structural Studies, Heinrich-Heine-University", "city": "Düsseldorf", "country": "Germany", "beamline_name": "Xenocs Xeuss 2.0 Q-Xoom", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Histone deacetylase 5", "short_name": "HDAC5", "sequence": "MLGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPALENLYFQ", "organism": "Homo sapiens", "uniprot_code": "Q9UQL6", "uniprot_range_first": 655, "uniprot_range_last": 1122, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELR\nGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQ\nQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIAS\nTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKL\nPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEI\nTGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQ\nFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMST\nSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATS\nMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGE\nLPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEED\nCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQ\nSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQE\nTGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLP\nCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAM\nGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNF\nFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVS\nAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVS\nALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGET\nEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL", "mw": 51.059, "total_mw": 51.059, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "Last amino acids (ENLYFQ) are the TEV cleavage site." } ], "buffer": { "name": "25 mM HEPES, 150 mM KCl, 1 mM DTT,", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Histone deacetylase 5 (HDAC5)", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Düsseldorf, Germany", "full_name": "Heinrich Heine University Düsseldorf", "webpage": "https://www.uni-duesseldorf.de" } ], "contributor_name": "Jens", "contributor_surname": "Reiners", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2024-02-29", "storage_temperature": 10.0, "cell_temperature": 15.0, "exposure_time": 600.0, "number_of_frames": 24, "wavelength": 0.154, "sample_detector_distance": 0.55, "concentration_min": null, "concentration_max": 2.15, "sample_volume": null, "flow_rate": null, "s_min": 0.128, "s_max": 4.795, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "1/cm", "experimental_mw": 52.9, "experimental_mw_error": null, "guinier_i0_mw": 49.85, "guinier_i0_mw_error": null, "porod_mw": 50.0, "porod_mw_error": null, "pddf_i0": 0.03615, "pddf_i0_error": null, "guinier_i0": 0.0361669, "guinier_i0_error": null, "pddf_rg": 2.891, "pddf_rg_error": null, "guinier_rg": 2.81, "guinier_rg_error": 0.029, "pddf_dmax": 10.1, "pddf_dmax_error": null, "porod_volume": 82.47, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 57, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDXL6_datloglog_img.png", "symmetry": null, "last_modified": "2026-02-27T13:41:15.416584+01:00", "bragg_peak": [] }