data_SASDFM2_MAIN # _sas_result.id 1300 _sas_result.SASBDB_code SASDFM2 _sas_result.experimental_MW 29.970 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 19.0200000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 9.750 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 4 _sas_result.Guinier_point_max 18 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 275-279 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)" _sas_scan.detc_id 15 _sas_scan.id 1300 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1261 _sas_scan.result_id 1300 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1261 _sas_sample.name "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1261 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 9.863206E+00 1.460082E+01 1300 2 5.968380E-03 1.662400E+01 7.106426E+00 1300 3 6.388220E-03 1.456380E+01 6.000712E+00 1300 4 6.808050E-03 1.703080E+01 4.066018E+00 1300 5 7.227880E-03 1.557900E+01 3.488520E+00 1300 6 7.647710E-03 1.755000E+01 3.063530E+00 1300 7 8.067540E-03 1.549780E+01 2.759300E+00 1300 8 8.487370E-03 1.584140E+01 2.474900E+00 1300 9 8.907200E-03 1.461392E+01 2.175328E+00 1300 10 9.327030E-03 1.331188E+01 2.003086E+00 1300 11 9.746860E-03 1.410266E+01 1.718136E+00 1300 12 1.016670E-02 1.310770E+01 1.531046E+00 1300 13 1.058650E-02 1.246524E+01 1.395594E+00 1300 14 1.100630E-02 1.360722E+01 1.352298E+00 1300 15 1.142620E-02 1.249116E+01 1.309458E+00 1300 16 1.184600E-02 1.247130E+01 1.227222E+00 1300 17 1.226580E-02 1.199228E+01 1.194790E+00 1300 18 1.268570E-02 1.136552E+01 1.062004E+00 1300 19 1.310550E-02 9.740980E+00 1.068792E+00 1300 20 1.352530E-02 1.059122E+01 9.680350E-01 1300 21 1.394520E-02 1.058172E+01 9.962486E-01 1300 22 1.436500E-02 1.005502E+01 9.514738E-01 1300 23 1.478480E-02 9.867940E+00 8.819498E-01 1300 24 1.520460E-02 9.366760E+00 8.716344E-01 1300 25 1.562450E-02 8.198460E+00 7.614858E-01 1300 26 1.604430E-02 8.219100E+00 7.386534E-01 1300 27 1.646410E-02 8.533600E+00 7.024718E-01 1300 28 1.688400E-02 8.421140E+00 6.590876E-01 1300 29 1.730380E-02 8.347020E+00 6.717092E-01 1300 30 1.772360E-02 7.256700E+00 6.304788E-01 1300 31 1.814350E-02 7.391580E+00 6.346558E-01 1300 32 1.856330E-02 7.095940E+00 6.057036E-01 1300 33 1.898310E-02 6.682280E+00 5.753866E-01 1300 34 1.940290E-02 6.540580E+00 5.737960E-01 1300 35 1.982280E-02 6.721600E+00 5.420670E-01 1300 36 2.024260E-02 6.351880E+00 5.303722E-01 1300 37 2.066240E-02 5.635280E+00 5.233484E-01 1300 38 2.108230E-02 5.906740E+00 5.259218E-01 1300 39 2.150210E-02 5.479520E+00 5.017660E-01 1300 40 2.192190E-02 5.647420E+00 4.803280E-01 1300 41 2.234180E-02 5.282680E+00 4.945434E-01 1300 42 2.276160E-02 4.863300E+00 4.586212E-01 1300 43 2.318140E-02 4.985060E+00 4.676984E-01 1300 44 2.360130E-02 4.909460E+00 4.363240E-01 1300 45 2.402110E-02 4.662060E+00 4.340024E-01 1300 46 2.444090E-02 4.723920E+00 4.416108E-01 1300 47 2.486070E-02 4.386320E+00 4.243372E-01 1300 48 2.528060E-02 4.205460E+00 4.243604E-01 1300 49 2.570040E-02 3.874660E+00 4.187620E-01 1300 50 2.612020E-02 3.953280E+00 4.151822E-01 1300 51 2.654010E-02 3.886380E+00 3.898772E-01 1300 52 2.695990E-02 3.856580E+00 3.882052E-01 1300 53 2.737970E-02 3.678120E+00 3.999544E-01 1300 54 2.779960E-02 3.650040E+00 3.849468E-01 1300 55 2.821940E-02 3.444120E+00 3.832704E-01 1300 56 2.863920E-02 3.260840E+00 3.850792E-01 1300 57 2.905900E-02 2.934800E+00 3.765352E-01 1300 58 2.947890E-02 3.084060E+00 3.889486E-01 1300 59 2.989870E-02 2.730140E+00 3.642260E-01 1300 60 3.031850E-02 3.328780E+00 3.829974E-01 1300 61 3.073840E-02 3.141960E+00 3.707242E-01 1300 62 3.115820E-02 2.771660E+00 3.822278E-01 1300 63 3.157800E-02 3.121340E+00 3.904600E-01 1300 64 3.199790E-02 2.607280E+00 3.744026E-01 1300 65 3.241770E-02 2.701200E+00 3.699182E-01 1300 66 3.283750E-02 2.557360E+00 3.531150E-01 1300 67 3.325730E-02 2.591260E+00 3.715556E-01 1300 68 3.367720E-02 2.300280E+00 3.578270E-01 1300 69 3.409700E-02 2.115780E+00 3.515176E-01 1300 70 3.451680E-02 2.322300E+00 3.730194E-01 1300 71 3.493670E-02 2.019680E+00 3.331348E-01 1300 72 3.535650E-02 2.278240E+00 3.535538E-01 1300 73 3.577630E-02 2.245960E+00 3.481034E-01 1300 74 3.619620E-02 1.803840E+00 3.422318E-01 1300 75 3.661600E-02 2.030500E+00 3.540098E-01 1300 76 3.703580E-02 1.890900E+00 3.257344E-01 1300 77 3.745560E-02 1.871440E+00 3.323098E-01 1300 78 3.787550E-02 1.609660E+00 3.212752E-01 1300 79 3.829530E-02 2.063940E+00 3.272882E-01 1300 80 3.871510E-02 1.694580E+00 3.226274E-01 1300 81 3.913500E-02 1.971180E+00 3.134400E-01 1300 82 3.955480E-02 1.609880E+00 3.141248E-01 1300 83 3.997460E-02 1.744340E+00 3.036826E-01 1300 84 4.039450E-02 1.558940E+00 3.117162E-01 1300 85 4.081430E-02 1.585440E+00 3.010396E-01 1300 86 4.123410E-02 1.553220E+00 2.933318E-01 1300 87 4.165390E-02 1.590140E+00 3.063028E-01 1300 88 4.207380E-02 1.554180E+00 2.968036E-01 1300 89 4.249360E-02 1.344080E+00 2.962550E-01 1300 90 4.291340E-02 1.463080E+00 2.824480E-01 1300 91 4.333330E-02 1.463180E+00 2.864556E-01 1300 92 4.375310E-02 1.309980E+00 2.936034E-01 1300 93 4.417290E-02 1.145260E+00 2.707780E-01 1300 94 4.459280E-02 1.397100E+00 2.748088E-01 1300 95 4.501260E-02 1.499920E+00 2.710332E-01 1300 96 4.543240E-02 1.086400E+00 2.702900E-01 1300 97 4.585220E-02 1.210180E+00 2.726188E-01 1300 98 4.627210E-02 1.295240E+00 2.682848E-01 1300 99 4.669190E-02 1.298760E+00 2.711154E-01 1300 100 4.711170E-02 1.218580E+00 2.659028E-01 1300 101 4.753160E-02 1.361200E+00 2.604666E-01 1300 102 4.795140E-02 1.178320E+00 2.626370E-01 1300 103 4.837120E-02 1.302780E+00 2.580198E-01 1300 104 4.879110E-02 1.028700E+00 2.597696E-01 1300 105 4.921090E-02 1.035620E+00 2.576924E-01 1300 106 4.963070E-02 7.855000E-01 2.506270E-01 1300 107 5.005050E-02 1.177200E+00 2.555248E-01 1300 108 5.047040E-02 9.003200E-01 2.518908E-01 1300 109 5.089020E-02 1.032860E+00 2.569672E-01 1300 110 5.131000E-02 1.026820E+00 2.525674E-01 1300 111 5.172990E-02 7.814800E-01 2.477484E-01 1300 112 5.214970E-02 8.574598E-01 2.461278E-01 1300 113 5.256950E-02 8.968200E-01 2.511550E-01 1300 114 5.298940E-02 9.671400E-01 2.460098E-01 1300 115 5.340920E-02 8.096802E-01 2.459364E-01 1300 116 5.382900E-02 1.057440E+00 2.450222E-01 1300 117 5.424890E-02 6.555392E-01 2.404038E-01 1300 118 5.466870E-02 6.533000E-01 2.395168E-01 1300 119 5.508850E-02 8.742400E-01 2.392028E-01 1300 120 5.550830E-02 1.067200E+00 2.413840E-01 1300 121 5.592820E-02 6.614000E-01 2.407862E-01 1300 122 5.634800E-02 8.212998E-01 2.369718E-01 1300 123 5.676780E-02 5.405204E-01 2.410512E-01 1300 124 5.718770E-02 8.105594E-01 2.306450E-01 1300 125 5.760750E-02 6.685798E-01 2.335146E-01 1300 126 5.802730E-02 6.939596E-01 2.290270E-01 1300 127 5.844720E-02 6.426198E-01 2.267086E-01 1300 128 5.886700E-02 5.770600E-01 2.283116E-01 1300 129 5.928680E-02 7.145202E-01 2.244060E-01 1300 130 5.970660E-02 6.199796E-01 2.292428E-01 1300 131 6.012650E-02 6.422406E-01 2.261148E-01 1300 132 6.054630E-02 6.309800E-01 2.206252E-01 1300 133 6.096610E-02 6.805400E-01 2.249094E-01 1300 134 6.138600E-02 5.997000E-01 2.244102E-01 1300 135 6.180580E-02 6.620000E-01 2.221534E-01 1300 136 6.222560E-02 6.820398E-01 2.183152E-01 1300 137 6.264550E-02 5.878000E-01 2.205694E-01 1300 138 6.306530E-02 4.449404E-01 2.172696E-01 1300 139 6.348510E-02 6.043600E-01 2.157702E-01 1300 140 6.390490E-02 5.923600E-01 2.163982E-01 1300 141 6.432480E-02 5.926998E-01 2.184134E-01 1300 142 6.474460E-02 6.534200E-01 2.154848E-01 1300 143 6.516440E-02 4.215004E-01 2.126214E-01 1300 144 6.558430E-02 5.723602E-01 2.116098E-01 1300 145 6.600410E-02 7.560802E-01 2.169310E-01 1300 146 6.642390E-02 5.645406E-01 2.121326E-01 1300 147 6.684380E-02 4.279604E-01 2.087232E-01 1300 148 6.726360E-02 4.812800E-01 2.141022E-01 1300 149 6.768340E-02 5.044600E-01 2.085416E-01 1300 150 6.810320E-02 3.719198E-01 2.028258E-01 1300 151 6.852310E-02 5.590204E-01 2.117174E-01 1300 152 6.894290E-02 4.553002E-01 2.120996E-01 1300 153 6.936270E-02 4.780400E-01 2.047388E-01 1300 154 6.978260E-02 3.167600E-01 2.078248E-01 1300 155 7.020240E-02 4.882600E-01 2.075718E-01 1300 156 7.062220E-02 4.572408E-01 2.006898E-01 1300 157 7.104210E-02 5.681002E-01 2.070850E-01 1300 158 7.146190E-02 4.450392E-01 1.997918E-01 1300 159 7.188170E-02 4.282200E-01 2.012328E-01 1300 160 7.230160E-02 3.743800E-01 2.012358E-01 1300 161 7.272140E-02 5.112804E-01 2.027688E-01 1300 162 7.314120E-02 5.390790E-01 2.022490E-01 1300 163 7.356100E-02 5.535004E-01 1.994198E-01 1300 164 7.398090E-02 4.113604E-01 2.015542E-01 1300 165 7.440070E-02 4.748196E-01 1.962286E-01 1300 166 7.482050E-02 5.254202E-01 1.981836E-01 1300 167 7.524040E-02 3.812600E-01 1.941406E-01 1300 168 7.566020E-02 5.344000E-01 1.968906E-01 1300 169 7.608000E-02 5.701804E-01 2.029018E-01 1300 170 7.649980E-02 4.789400E-01 1.959082E-01 1300 171 7.691970E-02 4.229794E-01 1.975686E-01 1300 172 7.733950E-02 5.020000E-01 1.954818E-01 1300 173 7.775930E-02 2.869597E-01 1.896650E-01 1300 174 7.817920E-02 4.591000E-01 1.974428E-01 1300 175 7.859900E-02 4.201404E-01 1.924822E-01 1300 176 7.901880E-02 3.902796E-01 1.967390E-01 1300 177 7.943870E-02 3.123398E-01 1.933000E-01 1300 178 7.985850E-02 3.993404E-01 1.925242E-01 1300 179 8.027830E-02 4.090606E-01 1.925114E-01 1300 180 8.069810E-02 3.106998E-01 1.892702E-01 1300 181 8.111800E-02 2.837400E-01 1.954268E-01 1300 182 8.153780E-02 3.652406E-01 1.882736E-01 1300 183 8.195760E-02 3.604798E-01 1.905646E-01 1300 184 8.237750E-02 4.582000E-01 1.910236E-01 1300 185 8.279730E-02 2.329796E-01 1.881788E-01 1300 186 8.321710E-02 5.262198E-01 1.906898E-01 1300 187 8.363700E-02 3.394200E-01 1.923578E-01 1300 188 8.405680E-02 2.696200E-01 1.982392E-01 1300 189 8.447660E-02 1.472403E-01 1.997840E-01 1300 190 8.489650E-02 3.248402E-01 1.975568E-01 1300 191 8.531630E-02 3.734002E-01 2.032306E-01 1300 192 8.573610E-02 3.415002E-01 1.998762E-01 1300 193 8.615590E-02 3.989594E-01 2.034566E-01 1300 194 8.657580E-02 2.798805E-01 2.073906E-01 1300 195 8.699560E-02 3.550003E-01 2.055834E-01 1300 196 8.741540E-02 4.370794E-01 2.031616E-01 1300 197 8.783530E-02 3.775998E-01 2.053700E-01 1300 198 8.825510E-02 4.739198E-01 2.071920E-01 1300 199 8.867490E-02 4.225400E-01 2.154614E-01 1300 200 8.909480E-02 4.499392E-01 2.075038E-01 1300 201 8.951460E-02 3.999593E-01 2.060032E-01 1300 202 8.993440E-02 2.709000E-01 2.046192E-01 1300 203 9.035420E-02 3.678398E-01 2.066918E-01 1300 204 9.077410E-02 2.430000E-01 2.039726E-01 1300 205 9.119390E-02 3.564004E-01 2.016094E-01 1300 206 9.161370E-02 3.144206E-01 2.048770E-01 1300 207 9.203360E-02 3.671598E-01 2.110640E-01 1300 208 9.245340E-02 4.118606E-01 2.100270E-01 1300 209 9.287320E-02 2.690402E-01 2.061442E-01 1300 210 9.329310E-02 3.689203E-01 2.110824E-01 1300 211 9.371290E-02 3.520202E-01 2.140862E-01 1300 212 9.413270E-02 3.307606E-01 2.122480E-01 1300 213 9.455250E-02 1.263608E-01 2.107704E-01 1300 214 9.497240E-02 2.386398E-01 2.100508E-01 1300 215 9.539220E-02 3.547002E-01 2.054672E-01 1300 216 9.581200E-02 4.018602E-01 2.077078E-01 1300 217 9.623190E-02 3.151000E-01 2.074280E-01 1300 218 9.665170E-02 2.233798E-01 2.008120E-01 1300 219 9.707150E-02 3.169198E-01 2.034298E-01 1300 220 9.749140E-02 3.177604E-01 1.989556E-01 1300 221 9.791120E-02 3.411603E-01 2.001510E-01 1300 222 9.833100E-02 4.933596E-01 2.017496E-01 1300 223 9.875080E-02 1.339597E-01 1.983772E-01 1300 224 9.917070E-02 2.938398E-01 1.979220E-01 1300 225 9.959050E-02 2.065209E-01 1.954034E-01 1300 226 1.000100E-01 2.268203E-01 1.928636E-01 1300 227 1.004300E-01 2.583398E-01 1.985236E-01 1300 228 1.008500E-01 1.667005E-01 1.915776E-01 1300 229 1.012700E-01 3.332802E-01 1.982954E-01 1300 230 1.016900E-01 3.905998E-01 1.943642E-01 1300 231 1.021090E-01 2.784802E-01 1.944668E-01 1300 232 1.025290E-01 2.315398E-01 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