{ "code": "SASDBM6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "TIA-1 RRM23 binding and recognition of target oligonucleotides.", "publication": { "title": "TIA-1 RRM23 binding and recognition of target oligonucleotides.", "author_list": "Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA", "journal": "Nucleic Acids Res", "doi": "10.1093/nar/gkx102", "pmid": "28184449", "published_date": "2017 May 5" }, "status": "released", "submitted_date": "2016-09-22", "released_date": "2017-03-28" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDBM6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDBM6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBM6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBM6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDBM6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDBM6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDBM6.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "Australian Synchrotron", "city": "Melbourne", "country": "Australia", "beamline_name": "SAXS/WAXS", "beam_geometry": "Point", "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Nucleolysin TIA-1 isoform p40", "short_name": "TIA-1", "sequence": "ENLYFQGAMGNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGK", "organism": "Homo sapiens", "uniprot_code": "P31483", "uniprot_range_first": 93, "uniprot_range_last": 274, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAA\nAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTE\nDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTN\nWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQI\nMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPVQQQNQI\nGYPQPYGQWGQWYGNAQQIGQYMPNGWQVPAYGMYGQAWNQQGFNQTQSSAPWMGPNYGV\nQPPQGQNGSMLPNQPSGYRVAGYETQ", "mw": 21.325, "total_mw": 21.325, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM HEPES, 100 mM NaCl, 3% v/v glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.0, "deuteration": null }, "purity_method": null, "name": "Nucleolysin TIA-1 isoform p40", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Melbourne, Australia", "full_name": "Monash University", "webpage": "http://www.monash.edu/" } ], "contributor_name": "Matthew", "contributor_surname": "Wilce", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2015-07-02", "storage_temperature": 4.0, "cell_temperature": 15.0, "exposure_time": 1.0, "number_of_frames": 30, "wavelength": 0.1033, "sample_detector_distance": 1.6, "concentration_min": null, "concentration_max": 2.5, "sample_volume": null, "flow_rate": null, "s_min": 0.377, "s_max": 3.615, "total_exposure_time": null, "seccolumn": null }, "fits": [], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "GNOM", "i0_calibration_standard": null, "description": "apo-TIA-1 RRM23", "experiment_description": "Synchrotron SAXS data from a solution of nucleolysin TIA-1 (isoform p40; amino acids 93-274) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7, were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.", "tags": [], "intensity_unit": null, "experimental_mw": 20.2, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": null, "porod_mw_error": null, "pddf_i0": 0.03491, "pddf_i0_error": 0.002, "guinier_i0": 0.034, "guinier_i0_error": 0.0003, "pddf_rg": 2.49, "pddf_rg_error": 0.2, "guinier_rg": 2.32, "guinier_rg_error": 0.22, "pddf_dmax": 8.8, "pddf_dmax_error": null, "porod_volume": 26.3, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 36, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDBM6_datloglog_img.png", "symmetry": null, "last_modified": "2020-07-22T10:39:57.780614+02:00", "bragg_peak": [] }