{ "code": "SASDDM7", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.", "publication": { "title": "Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.", "author_list": "Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C", "journal": "PLoS One", "doi": "10.1371/journal.pone.0191162", "pmid": "29324815", "published_date": "2018" }, "status": "released", "submitted_date": "2018-05-16", "released_date": "2018-06-05" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDM7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDM7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDM7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDM7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDM7.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "anti-DISC1 single-domain camelid antibody VHH B5", "short_name": "VHH B5", "sequence": "MAQVKLEESGGGLVQAGGSLRLSCAASGTLFSISVMGWYRQAPGMQRELVGSITDGGSTI\r\nYGDSVKGRFAISKDNAKRTIYLQMNSLKPEDTAVYYCNYRRLTSNYWGQGTQVTVSSAAA\r\nLEHHHHHH", "organism": null, "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 13.924, "total_mw": 13.924, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836", "short_name": "DISC1 12D2", "sequence": "MGHHHHHHDYDIPTTENLYFQGWEADLEACRLLIQSLQLQEARGSLSVEDE\r\nRQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLHCAGGEQKEESYILSAELG\r\nEKCEDIGKKLLYLEDQLHTAIHSHDEDLIQSLRRELQMVKETLQAMILQLQPAKEAGSG", "organism": null, "uniprot_code": "Q9NRI5", "uniprot_range_first": 691, "uniprot_range_last": 836, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPA\nVGTLFRFPGGVSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGI\nQLRGGTRLPDRLSWPCGPGSAGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSA\nELSSNSCSPGCGPEVPPTPPGSHSAFTSSFSFIRLSLGSAGERGEAEGCPPSREAESHCQ\nSPQEMGAKAASLDGPHEDPRCLSRPFSLLATRVSADLAQAARNSSRPERDMHSLPDMDPG\nSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEVISLRLKLQKL\nQEDAVENDDYDKAETLQQRLEDLEQEKISLHFQLPSRQPALSSFLGHLAAQVQAALRRGA\nTQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWLLQEKQQLQKEIEALQARMFVLE\nAKDQQLRREIEEQEQQLQWQGCDLTPLVGQLSLGQLQEVSKALQDTLASAGQIPFHAEPP\nETIRSLQERIKSLNLSLKEITTKVCMSEKFCSTLRKKVNDIETQLPALLEAKMHAISGNH\nFWTAKDLTEEIRSLTSEREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAY\nETSVKENTMKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQSLQLQEARGSLSVEDER\nQMDDLEGAAPPIPPRLHSEDKRKTPLKVLEEWKTHLIPSLHCAGGEQKEESYILSAELGE\nKCEDIGKKLLYLEDQLHTAIHSHDEDLIQSLRRELQMVKETLQAMILQLQPAKEAGEREA\nAASCMTAGVHEAQA", "mw": 19.426, "total_mw": 19.426, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Tris-HCl, 150 mM NaCl, 1mM DTT", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Germany", "full_name": "Forschungszentrum Jülich", "webpage": "https://www.fz-juelich.de" } ], "contributor_name": "Andreas", "contributor_surname": "Stadler", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2016-02-19", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 1.0, "number_of_frames": 10, "wavelength": 0.1, "sample_detector_distance": null, "concentration_min": null, "concentration_max": 2.35, "sample_volume": null, "flow_rate": null, "s_min": 0.08, "s_max": 4.945, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDM7_fit1_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "2.8.3", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDM7_fit1_model1.pdb", "model_mw": 52.5, "bead_radius": 2.2, "log": null, "symmetry": null, "comment": null, "user": 317 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDM7_fit1_model2_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "2.8.3", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDM7_fit1_model2.pdb", "model_mw": 52.563, "bead_radius": 2.75, "log": null, "symmetry": null, "comment": null, "user": 317 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDM7_fit1_fit_img.png", "software": "DAMMIF", "chi_square_value": 1.696, "p_value": 0.000475, "fit_residual_plot": "SASDDM7_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDM7_fit1.fir", "fit_log": null, "software_version": "2.8.3", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "Synchrotron SAXS data from solutions of the complex formed between the disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and the anti-DISC1 single-domain camelid antibody VHH B5 in 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.35 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 36.2, "experimental_mw_error": null, "guinier_i0_mw": 36.2, "guinier_i0_mw_error": null, "porod_mw": 45.2, "porod_mw_error": null, "pddf_i0": 68.35, "pddf_i0_error": null, "guinier_i0": 68.06, "guinier_i0_error": 0.08, "pddf_rg": 3.146, "pddf_rg_error": null, "guinier_rg": 3.07, "guinier_rg_error": 0.02, "pddf_dmax": 10.35, "pddf_dmax_error": null, "porod_volume": 76.8, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 29, "guinier_point_last": 73, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDM7_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }