{ "code": "SASDAM8", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.", "publication": { "title": "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.", "author_list": "Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE", "journal": "Nucleic Acids Res", "doi": "10.1093/nar/gkv987", "pmid": "26424851", "published_date": "2015 Nov 16" }, "status": "released", "submitted_date": "2015-07-06", "released_date": "2016-03-17" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDAM8.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAM8.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAM8_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAM8_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAM8_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAM8_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDAM8.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Latency-associated nuclear antigen", "short_name": "mLANA", "sequence": "MPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGTQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGPPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPYTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEKYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGSPGSSPGQQGQGSQT", "organism": "Murid herpesvirus 4", "uniprot_code": "O41974", "uniprot_range_first": 124, "uniprot_range_last": 316, "oligomerization": "tetramer", "molecular_type": "protein", "uniprot_sequence": "MPTSPPTTRNTTSGKTRSGCKRRCFNKPAAMPPKRRRAPKRPAPPPPPGCQGDEESSQGT\nQTPNPPSPPVPPSSPTLPSSPVPPSSPVHEPPSPSPPPAPPSPDVDVEGLDVGETDDPGP\nPPPKRYSRYQKPHNPSDPLPKKYQGMRRHLQVTAPRLFDPEGHPPTHFKSAVMFSSTHPY\nTLNKLHKCIQSKHVLSTPVSCLPLVPGTTQQCVTYYLLSFVEDKKQAKKLKRVVLAYCEK\nYHSSVEGTIVKAKPYFPLPEPPTEPPTDPEQPSTSTQASGTQHGPTASLDAGAEQGATGS\nPGSSPGQQGQGSQT", "mw": 21.721, "total_mw": 86.885, "number_molecules": 4, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "25 mM Na/K Phosphate", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "MHV-68 LANA", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": false }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Maxim", "contributor_surname": "Petoukhov", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2013-04-27", "storage_temperature": null, "cell_temperature": 10.0, "exposure_time": 0.05, "number_of_frames": 20, "wavelength": 0.12, "sample_detector_distance": 3.1, "concentration_min": null, "concentration_max": 5.0, "sample_volume": null, "flow_rate": null, "s_min": 0.188, "s_max": 4.609, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAM8_fit1_model1_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAM8_fit1_model1.pdb", "model_mw": 89.13, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 102 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAM8_fit1_model2_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAM8_fit1_model2.pdb", "model_mw": 89.13, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 102 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAM8_fit1_model3_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAM8_fit1_model3.pdb", "model_mw": 89.13, "bead_radius": 1.9, "log": null, "symmetry": "", "comment": "", "user": 102 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAM8_fit1_fixed_fit_img.png", "software": null, "chi_square_value": 1.69, "p_value": 0.0, "fit_residual_plot": "fit4columns287_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDAM8_fit1.fit", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": "Study of oligomeric states of mLANA and kLANA and their complexes with DNA", "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 100.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 100.0, "porod_mw_error": null, "pddf_i0": null, "pddf_i0_error": null, "guinier_i0": 4845.0, "guinier_i0_error": null, "pddf_rg": 4.4, "pddf_rg_error": null, "guinier_rg": 4.2, "guinier_rg_error": null, "pddf_dmax": 16.0, "pddf_dmax_error": null, "porod_volume": 117.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": null, "guinier_point_last": null, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDAM8_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }