{ "code": "SASDCM8", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.", "publication": { "title": "Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.", "author_list": "Krausze J, Probst C, Curth U, Reichelt J, Saha S, Schafflick D, Heinz DW, Mendel RR, Kruse T", "journal": "Biochem J", "doi": "10.1042/BCJ20160846", "pmid": "27803248", "published_date": "2017 Jan 1" }, "status": "released", "submitted_date": "2017-10-26", "released_date": "2021-02-08" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCM8.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCM8.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCM8_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCM8_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCM8.sascif", "experiment": { "instrument": { "detector": { "type": "Dectris", "name": "Pilatus 1M", "resolution": 172.0 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "ID14-3", "beam_geometry": "", "type_of_source": "X-ray synchrotron", "point_source": true, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Molybdopterin biosynthesis protein CNX1", "short_name": "Cnx1E", "sequence": "MSRGSMEGQGCCGGGGGKTEMIPTEEALRIVFGVSKRLPPVIVSLYEALGKVLAEDIRAP\r\nDPLPPYPASVKDGYAVVASDGPGEYPVITESRAGNDGLGVTVTPGTVAYVTTGGPIPDGA\r\nDAVVQVEDTKVIGDVSTESKRVKILIQTKKGTDIRRVGCDIEKDATVLTTGERIGASEIG\r\nLLATAGVTMVKVYPMPIVAILSTGDELVEPTAGTLGRGQIRDSNRAMLVAAVMQQQCKVV\r\nDLGIVRDDRKELEKVLDEAVSSGVDIILTSGGVSMGDRDFVKPLLEEKGKVYFSKVLMKP\r\nGKPLTFAEIRAKPTESMLGKTVLAFGLPGNPVSCLVCFNIFVVPTIRQLAGWTSPHPLRV\r\nRLRLQEPIKSDPIRPEFHRAIIKWKDNDGSGTPGFVAESTGHQMSSRLLSMRSANALLEL\r\nPATGNVLSAGSSVSAIIVSDISAFSIDKKASLSEPGSTSGGSAWSHPQFEK", "organism": "Arabidopsis thaliana", "uniprot_code": "Q39054", "uniprot_range_first": 1, "uniprot_range_last": 451, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MEGQGCCGGGGGKTEMIPTEEALRIVFGVSKRLPPVIVSLYEALGKVLAEDIRAPDPLPP\nYPASVKDGYAVVASDGPGEYPVITESRAGNDGLGVTVTPGTVAYVTTGGPIPDGADAVVQ\nVEDTKVIGDVSTESKRVKILIQTKKGTDIRRVGCDIEKDATVLTTGERIGASEIGLLATA\nGVTMVKVYPMPIVAILSTGDELVEPTAGTLGRGQIRDSNRAMLVAAVMQQQCKVVDLGIV\nRDDRKELEKVLDEAVSSGVDIILTSGGVSMGDRDFVKPLLEEKGKVYFSKVLMKPGKPLT\nFAEIRAKPTESMLGKTVLAFGLPGNPVSCLVCFNIFVVPTIRQLAGWTSPHPLRVRLRLQ\nEPIKSDPIRPEFHRAIIKWKDNDGSGTPGFVAESTGHQMSSRLLSMRSANALLELPATGN\nVLSAGSSVSAIIVSDISAFSIDKKASLSEPGSIRKEKKYDEVPGPEYKVAILTVSDTVSA\nGAGPDRSGPRAVSVVDSSSEKLGGAKVVATAVVPDEVERIKDILQKWSDVDEMDLILTLG\nGTGFTPRDVTPEATKKVIERETPGLLFVMMQESLKITPFAMLSRSAAGIRGSTLIINMPG\nNPNAVAECMEALLPALKHALKQIKGDKREKHPKHIPHAEATLPTDTWDQSYKSAYETGEK\nKEEAGCSCTH", "mw": 49.7, "total_mw": 99.4, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Hepes/KOH, 150 mM NaCl, 1 mM EDTA, 2 % (v/v) glycerol", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "Mo-insertase domain Cnx1E from Arabidopsis thaliana", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Braunschweig, Germany", "full_name": "Braunschweig University of Technology", "webpage": "https://www.tu-braunschweig.de/" } ], "contributor_name": "Joern", "contributor_surname": "Krausze", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2015-02-05", "storage_temperature": 4.0, "cell_temperature": 20.0, "exposure_time": 1.0, "number_of_frames": 10, "wavelength": 0.09919, "sample_detector_distance": 2.864, "concentration_min": 1.65, "concentration_max": 12.5, "sample_volume": null, "flow_rate": null, "s_min": 0.051, "s_max": 4.975, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCM8_fit1_model1_img.png", "software": "PDB (Protein Data Bank)", "pdb_link": [ { "pdb_code": "5g2r", "ext_code": "5G2R", "ext_type": "pdb", "difference_with_model": "derived" } ], "model_title": null, "type_of_model": "mix", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCM8_fit1_model1.pdb", "model_mw": 97.62, "bead_radius": 1.9, "log": null, "symmetry": "C2", "comment": "C2 symmetry generated from crystallographic symmetry", "user": 247 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCM8_fit1_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 39.748, "p_value": 0.0, "fit_residual_plot": "fit4columns1144_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCM8_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/SAXS_AB_maestro_17102600.log", "software_version": "2.8.2", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "4.6", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 99.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 105.9, "porod_mw_error": null, "pddf_i0": 69.92, "pddf_i0_error": 0.029, "guinier_i0": 68.97, "guinier_i0_error": 0.044, "pddf_rg": 3.805, "pddf_rg_error": 0.004, "guinier_rg": 3.64, "guinier_rg_error": 0.06, "pddf_dmax": 15.0, "pddf_dmax_error": null, "porod_volume": 180.59, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 23, "guinier_point_last": 54, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCM8_datloglog_img.png", "symmetry": null, "last_modified": "2021-02-08T20:12:23.492874+01:00", "bragg_peak": [] }