{ "code": "SASDYM9", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/nm", "project": { "title": "Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase", "publication": { "title": "Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase", "author_list": "Busch M, Loschwitz J, Papadopoulos A, Reiners J, Steinchen W, Calvagna V, Smits S, Jaeger K, Kedrov A", "journal": "Journal of Biological Chemistry", "doi": "10.1016/j.jbc.2026.113266", "pmid": null, "published_date": "2026 Jun" }, "status": "released", "submitted_date": "2026-03-08", "released_date": "2026-06-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYM9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYM9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYM9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYM9.sascif", "experiment": { "instrument": { "detector": { "type": "pixel counting", "name": "Pilatus3 R 300K", "resolution": 172.0 }, "name": "Center for Structural Studies, Heinrich-Heine-University", "city": "Düsseldorf", "country": "Germany", "beamline_name": "Xenocs Xeuss 2.0 Q-Xoom", "beam_geometry": null, "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Lipase chaperone", "short_name": "LipH VD", "sequence": "MGHHHHHHHHHHSSGHIDDDDKHMWLEPSPAPETAPPASPQAGAVHAPPAASAGEAVPAPQVMPAKVAPLPTSFRGTSVDGSFSVDASGNLLITRDIRNLFDYFLSAVGEEPLQQSLDRLRAYIAAELQEPARGQALALMQQYIDYKKELVLLERDLPRLADLDALRQREAAVKALRARIFSNEAHVAFFADEETYNQFTLERLAIRQDGKLSAEEKAAAIDRLRASLPEDQQESVLPQLQSELQQQTAALQAAGAGPEAIRQMRQQLVGAEATTRLEQLDRQRSAWKGRLDDYFAEKSRIEGNTGLSEADRRAAVERLAEERFSEQERLRLGALEQMRQAEQR", "organism": "Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)", "uniprot_code": "Q01725", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MKKILLLIPLAFAASLAWFVWLEPSPAPETAPPASPQAGADRAPPAASAGEAVPAPQVMP\nAKVAPLPTSFRGTSVDGSFSVDASGNLLITRDIRNLFDYFLSAVGEEPLQQSLDRLRAYI\nAAELQEPARGQALALMQQYIDYKKELVLLERDLPRLADLDALRQREAAVKALRARIFSNE\nAHVAFFADEETYNQFTLERLAIRQDGKLSAEEKAAAIDRLRASLPEDQQESVLPQLQSEL\nQQQTAALQAAGAGPEAIRQMRQQLVGAEATTRLEQLDRQRSAWKGRLDDYFAEKSRIEGN\nTGLSEADRRAAVERLAEERFSEQERLRLGALEQMRQAEQR", "mw": 38.321, "total_mw": 38.321, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "LipH without the N-terminal transmembrane helix." } ], "buffer": { "name": "50 mM Tris, 100 mM NaCl, 100 µM TCEP, 5% glycerol,", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Lipase chaperone (LipH) VD", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Düsseldorf, Germany", "full_name": "Heinrich Heine University Düsseldorf", "webpage": "https://www.uni-duesseldorf.de" } ], "contributor_name": "Jens", "contributor_surname": "Reiners", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2019-11-18", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 600.0, "number_of_frames": 24, "wavelength": 0.154, "sample_detector_distance": 0.55, "concentration_min": null, "concentration_max": 11.82, "sample_volume": null, "flow_rate": null, "s_min": 0.088, "s_max": 5.321, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDYM9_fit1_model1_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": "RANCH version 2.1 - 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