{ "code": "SASDDN6", "status": "Published", "type_of_curve": "Single concentration", "angular_unit": "1/A", "project": { "title": "Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding.", "publication": { "title": "Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding.", "author_list": "Trcka F, Durech M, Vankova P, Chmelik J, Martinkova V, Hausner J, Kadek A, Marcoux J, Klumpler T, Vojtesek B, Muller P, Man P", "journal": "Mol Cell Proteomics", "doi": "10.1074/mcp.RA118.001044", "pmid": "30459217", "published_date": "2018 Nov 20" }, "status": "released", "submitted_date": "2018-05-17", "released_date": "2018-11-30" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDDN6.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDDN6.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDDN6_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDDN6_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDDN6.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 100K", "resolution": 172.0 }, "name": "CEITEC", "city": "Brno", "country": "Czech Republic", "beamline_name": "Rigaku BioSAXS-1000", "beam_geometry": "", "type_of_source": "X-ray in house", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Heat shock 70 kDa protein 1", "short_name": "HSPA1A_T204A", "sequence": "MSYYHHHHHHLESTSLYKKAGFGSENLYFQSMAKAAAIGI\r\nDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTER\r\nLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKH\r\nWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKE\r\nIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNV\r\nLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGAFDVSI\r\nLTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKH\r\nKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFE\r\nGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKA\r\nQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAV\r\nAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGV\r\nMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAM\r\nTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVT\r\nATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAED\r\nEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKK\r\nVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISG\r\nLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD", "organism": "Homo sapiens", "uniprot_code": "P0DMV8", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "dimer", "molecular_type": "protein", "uniprot_sequence": "MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA\nLNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEIS\nSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAA\nIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNH\nFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRA\nRFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLN\nKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTI\nPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDI\nDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKN\nALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE\nQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD", "mw": 73.74, "total_mw": 147.48, "number_molecules": 2, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "50mM HEPES, 150mM KCH3COO, 2mM MgCl2", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.5, "deuteration": null }, "purity_method": null, "name": "Human stress-inducible heat shock 70 kDa protein 1, Hsp70 (Hspa1a) T204A mutant dimer, in the presence of ATP", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": null, "full_name": "CEITEC - Central European Institute of Technology, Masaryk University", "webpage": "https://www.ceitec.eu/" } ], "contributor_name": "Tomas", "contributor_surname": "Klumpler", "orcid": null } ], "concentration_method": null, "concentration_unit": null, "date": "2018-02-20", "storage_temperature": 4.0, "cell_temperature": 4.0, "exposure_time": 3600.0, "number_of_frames": null, "wavelength": 0.154, "sample_detector_distance": 0.48, "concentration_min": null, "concentration_max": 2.5, "sample_volume": null, "flow_rate": null, "s_min": 0.138, "s_max": 6.314, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDDN6_fit1_model1_img.png", "software": "MODELLER", "pdb_link": [], "model_title": null, "type_of_model": "atomic", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDDN6_fit1_model1.pdb", "model_mw": 148.5, "bead_radius": null, "log": null, "symmetry": null, "comment": null, "user": 195 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDDN6_fit1_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.15, "p_value": 0.489949, "fit_residual_plot": "SASDDN6_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDDN6_fit1.fit", "fit_log": null, "software_version": "2.8.3", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5", "i0_calibration_standard": null, "description": null, "experiment_description": "SAXS data from solutions of human stress-induced heat shock 70 kDa protein 1 ,Hsp70, (Hspa1a T204A mutant), with ATP in 50mM HEPES; 150mM KCH3COO; 2mM MgCl2, pH 7.5, were collected on a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 148.0, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 155.0, "porod_mw_error": null, "pddf_i0": 0.7457, "pddf_i0_error": 0.0058, "guinier_i0": 0.76, "guinier_i0_error": 0.011, "pddf_rg": 3.97, "pddf_rg_error": 0.2, "guinier_rg": 4.01, "guinier_rg_error": 0.077, "pddf_dmax": 11.56, "pddf_dmax_error": null, "porod_volume": 248.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 2, "guinier_point_last": 14, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDDN6_datloglog_img.png", "symmetry": null, "last_modified": "2019-12-06T13:07:15.624532+01:00", "bragg_peak": [] }