{ "code": "SASDAP7", "status": "Published", "type_of_curve": "Merged", "angular_unit": "1/nm", "project": { "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.", "publication": { "title": "The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.", "author_list": "Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C", "journal": "FEBS J", "doi": "10.1111/febs.13801", "pmid": "27390177", "published_date": "2016 Aug" }, "status": "released", "submitted_date": null, "released_date": "2016-07-25" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDAP7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDAP7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDAP7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDAP7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDAP7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus 2M", "resolution": null }, "name": "PETRA III", "city": "DESY; Hamburg", "country": "Germany", "beamline_name": "EMBL P12", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Pre-B-cell leukemia transcription factor 1", "short_name": "PBX1", "sequence": "MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDE\r\nAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGP\r\nEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVM\r\nNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNF\r\nNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQE\r\nEANIYAAK", "organism": "Homo sapiens", "uniprot_code": "P40424", "uniprot_range_first": 1, "uniprot_range_last": 308, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDE\nAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGP\nEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVM\nNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNF\nNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQE\nEANIYAAKTAVTATNVSAHGSQANSPSTPNSAGSSSSFNMSNSGDLFMSVQSLNGDSYQG\nAQVGANVQSQVDTLRHVISQTGGYSDGLAASQMYSPQGISANGGWQDATTPSSVTSPTEG\nPGSVHSDTSN", "mw": 34.455, "total_mw": 34.455, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null }, { "long_name": "Homeobox protein PKNOX1", "short_name": "PREP1", "sequence": "MMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYR\r\nHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVK\r\nAIQVLRIHLLELEKVNELCKDFCSRYIACLKTEMNSETLLSGEPGSPYSPVQSQQIQSAI\r\nTGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQ\r\nLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTN\r\nLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRF", "organism": "Homo sapiens", "uniprot_code": "P55347", "uniprot_range_first": 1, "uniprot_range_last": 344, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MMATQTLSIDSYQDGQQMQVVTELKTEQDPNCSEPDAEGVSPPPVESQTPMDVDKQAIYR\nHPLFPLLALLFEKCEQSTQGSEGTTSASFDVDIENFVRKQEKEGKPFFCEDPETDNLMVK\nAIQVLRIHLLELEKVNELCKDFCSRYIACLKTKMNSETLLSGEPGSPYSPVQSQQIQSAI\nTGTISPQGIVVPASALQQGNVAMATVAGGTVYQPVTVVTPQGQVVTQTLSPGTIRIQNSQ\nLQLQLNQDLSILHQDDGSSKNKRGVLPKHATNVMRSWLFQHIGHPYPTEDEKKQIAAQTN\nLTLLQVNNWFINARRRILQPMLDSSCSETPKTKKKTAQNRPVQRFWPDSIASGVAQPPPS\nELTMSEGAVVTITTPVNMNVDSLQSLSSDGATLAVQQVMMAGQSEDESVDSTEEDAGALA\nPAHISGLVLENSDSLQ", "mw": 38.339, "total_mw": 38.339, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT", "concentration_unit": "mM", "comment": null, "additive": "150 mM NaCl, 5% glycerol, 1 mM DTT", "concentration": 20.0, "pka": null, "ph": 7.4, "deuteration": null }, "purity_method": null, "name": "PBX1-PREB1 complex", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": "EMBL-Hamburg", "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany", "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation", "webpage": "http://www.embl-hamburg.de/index.php" } ], "contributor_name": "Erica", "contributor_surname": "Valentini", "orcid": null } ], "concentration_method": null, "concentration_unit": "mg/ml", "date": "2013-12-06", "storage_temperature": null, "cell_temperature": null, "exposure_time": 0.05, "number_of_frames": 50, "wavelength": 0.12, "sample_detector_distance": 3.1, "concentration_min": 1.5, "concentration_max": 2.8, "sample_volume": null, "flow_rate": null, "s_min": 0.028, "s_max": 4.45, "total_exposure_time": null, "seccolumn": null }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDAP7_fit1_model1_img.png", "software": "DAMMIF", "pdb_link": [], "model_title": null, "type_of_model": "dummy", "software_version": "", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDAP7_fit1_model1.pdb", "model_mw": 104.154, "bead_radius": 4.0, "log": null, "symmetry": "", "comment": "", "user": 1 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDAP7_fit1_fit_img.png", "software": null, "chi_square_value": 1.340964, "p_value": 7e-06, "fit_residual_plot": "SASDAP7_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDAP7_fit1.fir", "fit_log": null, "software_version": null, "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": null, "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": null, "experimental_mw": 77.0, "experimental_mw_error": null, "guinier_i0_mw": 77.0, "guinier_i0_mw_error": null, "porod_mw": 84.0, "porod_mw_error": null, "pddf_i0": 1580.0, "pddf_i0_error": null, "guinier_i0": 1580.53, "guinier_i0_error": null, "pddf_rg": 5.7, "pddf_rg_error": null, "guinier_rg": 5.846, "guinier_rg_error": null, "pddf_dmax": 19.0, "pddf_dmax_error": null, "porod_volume": 143.0, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 29, "guinier_point_last": 75, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDAP7_datloglog_img.png", "symmetry": null, "last_modified": "2023-06-21T07:56:39.492710+02:00", "bragg_peak": [] }