{ "code": "SASDYP9", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/nm", "project": { "title": "Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase", "publication": { "title": "Conformational dynamics of the membrane-anchored foldase LipH from Pseudomonas aeruginosa governs recognition and release of its client lipase", "author_list": "Busch M, Loschwitz J, Papadopoulos A, Reiners J, Steinchen W, Calvagna V, Smits S, Jaeger K, Kedrov A", "journal": "Journal of Biological Chemistry", "doi": "10.1016/j.jbc.2026.113266", "pmid": null, "published_date": "2026 Jun" }, "status": "released", "submitted_date": "2026-03-08", "released_date": "2026-06-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYP9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYP9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYP9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYP9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYP9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYP9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYP9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 2M", "resolution": 0.172 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "point", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Lipase chaperone", "short_name": "LipH VD", "sequence": "MGHHHHHHHHHHSSGHIDDDDKHMWLEPSPAPETAPPASPQAGAVHAPPAASAGEAVPAPQVMPAKVAPLPTSFRGTSVDGSFSVDASGNLLITRDIRNLFDYFLSAVGEEPLQQSLDRLRAYIAAELQEPARGQALALMQQYIDYKKELVLLERDLPRLADLDALRQREAAVKALRARIFSNEAHVAFFADEETYNQFTLERLAIRQDGKLSAEEKAAAIDRLRASLPEDQQESVLPQLQSELQQQTAALQAAGAGPEAIRQMRQQLVGAEATTRLEQLDRQRSAWKGRLDDYFAEKSRIEGNTGLSEADRRAAVERLAEERFSEQERLRLGALEQMRQAEQR", "organism": "Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)", "uniprot_code": "Q01725", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MKKILLLIPLAFAASLAWFVWLEPSPAPETAPPASPQAGADRAPPAASAGEAVPAPQVMP\nAKVAPLPTSFRGTSVDGSFSVDASGNLLITRDIRNLFDYFLSAVGEEPLQQSLDRLRAYI\nAAELQEPARGQALALMQQYIDYKKELVLLERDLPRLADLDALRQREAAVKALRARIFSNE\nAHVAFFADEETYNQFTLERLAIRQDGKLSAEEKAAAIDRLRASLPEDQQESVLPQLQSEL\nQQQTAALQAAGAGPEAIRQMRQQLVGAEATTRLEQLDRQRSAWKGRLDDYFAEKSRIEGN\nTGLSEADRRAAVERLAEERFSEQERLRLGALEQMRQAEQR", "mw": 38.321, "total_mw": 38.321, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "LipH without the N-terminal transmembrane helix." }, { "long_name": "Triacylglycerol lipase", "short_name": "LipA delta81", "sequence": "MTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAVLSGLVNSLGALISELSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSSL", "organism": "Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)", "uniprot_code": "P26876", "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSA\nLRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAV\nRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAVLSGLVNSLGALISFLSSGSTGTQN\nSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAF\nLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYR\nQHANRLKNASL", "mw": 21.307, "total_mw": 21.307, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "C-terminal truncated Version of LipA" } ], "buffer": { "name": "5 mM Tris, 5 mM glycine, 1 mM CaCl2, 5% glycerol,", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 9.0, "deuteration": null }, "purity_method": null, "name": "Lipase chaperone (LipH) VD : Triacylglycerol lipase (LipA) delta81 complex", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Düsseldorf, Germany", "full_name": "Heinrich Heine University Düsseldorf", "webpage": "https://www.uni-duesseldorf.de" } ], "contributor_name": "Jens", "contributor_surname": "Reiners", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2024-09-10", "storage_temperature": 20.0, "cell_temperature": 20.0, "exposure_time": 2.0, "number_of_frames": 1200, "wavelength": 0.099, "sample_detector_distance": 2.827, "concentration_min": null, "concentration_max": 1.0, "sample_volume": 100.0, "flow_rate": 0.6, "s_min": 0.163, "s_max": 4.081, "total_exposure_time": null, "seccolumn": 1 }, "fits": [ { "models": [], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDYP9_fit1_fixed_fit_img.png", "software": "EOM/GAJOE", "chi_square_value": 1.528, "p_value": 0.0, "fit_residual_plot": "SASDYP9_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDYP9_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Size_distr_001_1_WptfkyT.txt", "software_version": "GAJOE - version 2.1 - (r13837)", "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": null, "tags": [], "intensity_unit": "1/cm", "experimental_mw": 58.15, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 64.0, "porod_mw_error": null, "pddf_i0": 8.74, "pddf_i0_error": null, "guinier_i0": 8.83356, "guinier_i0_error": null, "pddf_rg": 3.529, "pddf_rg_error": null, "guinier_rg": 3.51, "guinier_rg_error": 0.043, "pddf_dmax": 11.7, "pddf_dmax_error": null, "porod_volume": 103.55, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 43, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDYP9_datloglog_img.png", "symmetry": null, "last_modified": "2026-06-25T14:59:43.664955+02:00", "bragg_peak": [] }