{ "code": "SASDCQ7", "status": "Published", "type_of_curve": "Other", "angular_unit": "1/A", "project": { "title": "Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.", "publication": { "title": "Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.", "author_list": "Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG", "journal": "Structure", "doi": "10.1016/j.str.2017.11.019", "pmid": "29276036", "published_date": "2018 Jan 2" }, "status": "released", "submitted_date": "2017-09-22", "released_date": "2018-01-02" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDCQ7.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDCQ7.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ7_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ7_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDCQ7_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDCQ7_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDCQ7.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "MAR 165 CCD", "resolution": null }, "name": "Advanced Photon Source (APS), Argonne National Laboratory", "city": "Lemont, IL", "country": "USA", "beamline_name": "BioCAT 18ID", "beam_geometry": null, "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Grp1 63-399 E161A Arf6 Q67L fusion protein", "short_name": null, "sequence": "MGHHHHHHGSTTQRNAQIAMGRKKFNMDPKKGIQFLIENDLLQSSPEDVAQFLYKGEGLNKTVIGDYLGER\r\nDDFNIKVLQAFVELHEFADLNLVQALRQFLWSFRLPGEAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVL\r\nSFAIIMLNTSLHNHNVRDKPTAERFITMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSH\r\nKGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKKGKVLSKIFGNKEMRILM\r\nLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGLDKIRPLWRHYYTGTQGLIFVV\r\nDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATS\r\nGDGLYEGLTWLTSNYN", "organism": "Mus musculus", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": null, "mw": 60.146, "total_mw": 60.146, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": null } ], "buffer": { "name": "20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 8.0, "deuteration": null }, "purity_method": null, "name": "Truncated monomeric Cytohesin-3 (Grp1; 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MSE converted to MET; PH domain and Arf6 derived from 2KAX", "user": 241 }, { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDCQ7_fit3_model2_img.png", "software": "Other", "pdb_link": [ { "pdb_code": "2r09", "ext_code": "2R09", "ext_type": "pdb", "difference_with_model": "derived" } ], "model_title": null, "type_of_model": "atomic", "software_version": "MultiFoXS", "model_data": "https://www.sasbdb.org/media/pdb_file/SASDCQ7_fit3_model2.pdb", "model_mw": 61.17, "bead_radius": 1.9, "log": null, "symmetry": "P1", "comment": "Sec7 domain derived from 2R09; MSE converted to MET; PH domain and Arf6 derived from 2KAX", "user": 241 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDCQ7_fit3_fitfoxs_img.png", "software": "Other", "chi_square_value": 1.62, "p_value": 0.000465, "fit_residual_plot": "SASDCQ7_fit3_fitfoxsresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDCQ7_fit3.dat", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/multifoxs_ensembles_size_G0vqVKs.txt", "software_version": "MultiFoXS", "description": null } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": null, "experiment_description": "This experimental profile was derived from SEC-SAXS data collected at the BioCAT Sector 18-ID beamline at the Argonne National Laboratory Advanced Photon Source using a MAR 165 CCD detector. The protein was equilibrated with a 1.2 molar excess of inositol 1,3,4,5-tetrakis phosphate (IP4), concentrated to 10 mg/ml, and injected in 0.1 ml onto a 3 ml Superdex-200 Increase column equilibrated with 20 mM Tris, pH 8.0, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, and 0.001 ml IP4. SAXS data sets were acquired with 1 s exposures at 5 s intervals during elution at a flow rate of 0.25 ml/min. Raw SAXS images were radially averaged on a log scale over the q range 0.00621-0.333 Å-1 and normalized by the incident beam intensity. The protein scattering profile was reconstructed by singular value decomposition and linear combination (SVD-LC) as described in Malaby et al. (2015) Methods for analysis of size-exclusion chromatography-small angle X-ray scattering and reconstruction of protein scattering. J Appl Crystallogr 48: 1102-1113.", "tags": [], "intensity_unit": "arbitrary", "experimental_mw": 57.5, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 57.5, "porod_mw_error": null, "pddf_i0": 1.01, "pddf_i0_error": 0.001, "guinier_i0": 1.01, "guinier_i0_error": 0.0008, "pddf_rg": 3.13, "pddf_rg_error": 0.007, "guinier_rg": 3.14, "guinier_rg_error": 0.01, "pddf_dmax": 11.6, "pddf_dmax_error": 0.1, "porod_volume": 86.2, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 31, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDCQ7_datloglog_img.png", "symmetry": null, "last_modified": "2020-08-28T15:28:09.410742+02:00", "bragg_peak": [] }