{ "code": "SASDYQ9", "status": "Published", "type_of_curve": "SEC-SAS", "angular_unit": "1/nm", "project": { "title": "ATPLyzer—An Advanced Ratiometric Multi‐Color Biosensor for Long‐Term Monitoring of ATP Dynamics", "publication": { "title": "ATPLyzer—An Advanced Ratiometric Multi‐Color Biosensor for Long‐Term Monitoring of ATP Dynamics", "author_list": "Papadopoulos A, Kaiser C, Schlumpberger P, Eßer J, Reiners J, Gertzen C, Grossmann G, Smits S", "journal": "Advanced Sensor Research", "doi": "10.1002/adsr.70171", "pmid": null, "published_date": "2026 Jun 27" }, "status": "released", "submitted_date": "2026-03-09", "released_date": "2026-06-09" }, "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYQ9.out", "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYQ9.dat", "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYQ9_dat_img.png", "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYQ9_kratky_img.png", "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYQ9_pofr_img.png", "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYQ9_guinier_img.png", "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYQ9.sascif", "experiment": { "instrument": { "detector": { "type": null, "name": "Pilatus3 2M", "resolution": 0.172 }, "name": "ESRF", "city": "Grenoble", "country": "France", "beamline_name": "BM29", "beam_geometry": "point", "type_of_source": "X-ray synchrotron", "point_source": null, "line_collimation": null, "sample_path_length": null, "line_collimation_slitlength": null, "line_collimation_integrationwidth": null, "xray_energy": null, "beam_profile_ah": null, "beam_profile_al": null }, "sample": { "molecule": [ { "long_name": "Ratiometric matryoshka multi-colour biosensor for ATP", "short_name": "ATPLyzer GO 1.7m apo", "sequence": "MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPMKTVKVNITTPDGPVYDADIEMVSVRAESGDLGILPGHIPTKAPLKIGAVRLKKDGQTEMVAVSGGTVEVRPDHVTINAQAAETAEGIDKERAEAARQRAQERLNSQTRYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTMVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGFQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALKGEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGGSASQGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLGDIDFKRAELALKRAMNRLSVAEMK", "organism": "synthetic construct", "uniprot_code": null, "uniprot_range_first": null, "uniprot_range_last": null, "oligomerization": "monomer", "molecular_type": "protein", "uniprot_sequence": "", "mw": 72.205, "total_mw": 72.205, "number_molecules": 1, "complex_state": false, "deuteration": null, "molecule_source": "biological", "molecule_description": "The sensor contains different domains:\r\n\t•\tε subunit of the bacterial FoF1 ATPase from Bacillus spec.\r\n\t•\tsuperfolder circularly permutated GFP (cpsfGFP (reporter fluorophore) from Aequorea victoria)\r\n\t•\tLSSmApple (reference fluorophore) from Discosoma sp.\r\nand is constructed as: \r\n1–39 N-Terminal 10xHis, T7tag, enterokinase site/XpressTag, 40–147 εFoF1-N-terminus of B. subtilis, 148–151 PASH Linker, 152–240 cpsfGFP-C-terminus, 241–243 GGT Linker, 244–478 LSSmApple, 479–481 GGS Linker, 482–625 cpsfGFP-N-terminus, 626–629 FNNP Linker, 630–708 εFoF1-N-terminus of B. PS3-C-terminus." } ], "buffer": { "name": "50 mM MOPS, 100 mM KCl, 1 mM MgCl2,", "concentration_unit": null, "comment": null, "additive": null, "concentration": null, "pka": null, "ph": 7.0, "deuteration": null }, "purity_method": null, "name": "ATPLyzer GO 1.7 m apo", "ext_coefficient": null, "contrast": null, "specific_vol": null, "dry_vol": null, "absorbption": null, "deuteration": null, "mixture": null }, "contributor": [ { "affiliation": [ { "short_name": null, "address": "Düsseldorf, Germany", "full_name": "Heinrich Heine University Düsseldorf", "webpage": "https://www.uni-duesseldorf.de" } ], "contributor_name": "Jens", "contributor_surname": "Reiners", "orcid": "" } ], "concentration_method": null, "concentration_unit": null, "date": "2023-11-30", "storage_temperature": 10.0, "cell_temperature": 10.0, "exposure_time": 3.0, "number_of_frames": 800, "wavelength": 0.099, "sample_detector_distance": 2.812, "concentration_min": null, "concentration_max": 4.9, "sample_volume": 100.0, "flow_rate": 0.6, "s_min": 0.136, "s_max": 4.757, "total_exposure_time": null, "seccolumn": 1 }, "fits": [ { "models": [ { "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDYQ9_fit1_model1_img.png", "software": "EOM/RANCH", "pdb_link": [], "model_title": null, "type_of_model": "mix", "software_version": null, "model_data": "https://www.sasbdb.org/media/pdb_file/SASDYQ9_fit1_model1.pdb", "model_mw": 72.11, "bead_radius": 1.9, "log": "https://www.sasbdb.org/media/log_files/06278eom00.log", "symmetry": "P1", "comment": null, "user": 402 } ], "fit_unit": "1/A", "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDYQ9_fit1_fixed_fit_img.png", "software": "CRYSOL", "chi_square_value": 1.069, "p_value": 0.0532, "fit_residual_plot": "SASDYQ9_fit1_fitresiduals_img.png", "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDYQ9_fit1.fit", "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/06278eom00.log", "software_version": "C R Y S O L Version 2.8.3", "description": "" } ], "estimated_volume_method": null, "pddf_software": "ATSAS GNOM", "pddf_software_version": "5.0", "i0_calibration_standard": null, "description": "Please read the molecule description by clicking the molecule link. Collected raw SAXS data from the ESRF (proposal ID MX-2485) can be found under: https://doi.org/10.15151/ESRF-DC-2387389777", "experiment_description": null, "tags": [], "intensity_unit": "1/cm", "experimental_mw": 71.13, "experimental_mw_error": null, "guinier_i0_mw": null, "guinier_i0_mw_error": null, "porod_mw": 68.0, "porod_mw_error": null, "pddf_i0": 31.83, "pddf_i0_error": null, "guinier_i0": 31.5566, "guinier_i0_error": null, "pddf_rg": 3.993, "pddf_rg_error": null, "guinier_rg": 3.81, "guinier_rg_error": 0.02, "pddf_dmax": 14.13, "pddf_dmax_error": null, "porod_volume": 109.86, "porod_volume_error": null, "estimated_volume": null, "estimated_volume_error": null, "guinier_point_first": 1, "guinier_point_last": 40, "pddf_point_first": null, "pddf_point_last": null, "i0_calibration_standard_data": null, "intensities_log_log_plot": "SASDYQ9_datloglog_img.png", "symmetry": null, "last_modified": "2026-06-30T10:43:52.011739+02:00", "bragg_peak": [] }