data_SASDFR2_MAIN # _sas_result.id 1304 _sas_result.SASBDB_code SASDFR2 _sas_result.experimental_MW 29.920 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod . _sas_result.MW_Porod_error . _sas_result.I0_from_PR . _sas_result.I0_from_PR_error . _sas_result.I0_from_Guinier 43.5000000000 _sas_result.I0_from_Guinier_error . _sas_result.Rg_from_PR . _sas_result.Rg_from_PR_error . _sas_result.Rg_from_Guinier 15.220 _sas_result.Rg_from_Guinier_error . _sas_result.D_max . _sas_result.Porod_volume . _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 2 _sas_result.Guinier_point_max 7 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments ;SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 275-279 ; # _sas_scan.title "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)" _sas_scan.detc_id 15 _sas_scan.id 1304 _sas_scan.measurement_date 2018-01-29 _sas_scan.storage_temperature 23.0 _sas_scan.cell_temperature 23.0 _sas_scan.exposure_time 1.000 _sas_scan.number_of_frames 500 _sas_scan.unit 1/A _sas_scan.qmin 0.0055 _sas_scan.qmax 0.4023 _sas_scan.sample_id 1265 _sas_scan.result_id 1304 _sas_scan.beam_id 30 # _sas_beam.id 30 _sas_beam.instrument_name "Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2" _sas_beam.instrument_city "Stanford, CA" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.100000 _sas_beam.dist_spec_to_detc 2.54000 # _sas_detc.id 15 _sas_detc.name "Rayonix MX225-HE" _sas_detc.type . _sas_detc.pixsize_x . # _sas_sample.id 1265 _sas_sample.name "Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 2.40 _sas_sample.concentration_method . _sas_sample.entity_id 1212 # _sas_buffer.id 653 _sas_buffer.name "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.500 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 1265 # _entity.id 1212 _entity.type protein _entity.pdbx_description "Cell wall synthesis protein Wag31" _entity.formula_weight 29.980 _entity.pdbx_number_of_molecules . # _entity_name_com.entity_id 1212 _entity_name_com.name . # _entity_poly.entity_id 1212 _entity_poly.pdbx_seq_one_letter_code ;MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL AAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT AKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA DALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA APVDSNADAGGFDQFNRGKN ; # _entity_src_gen.entity_id 1212 _entity_src_gen.gene_src_common_name "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)" # _struct_ref.id 1 _struct_ref.entity_id 1212 _struct_ref.db_name UniProt _struct_ref.db_code P9WMU1 _struct_ref.details http://www.uniprot.org/uniprot/P9WMU1 # _pdbx_contact_author.id 299 _pdbx_contact_author.name_first Komal _pdbx_contact_author.name_last Choukate _pdbx_contact_author.role . _pdbx_contact_author.address_1 CSIR _pdbx_contact_author.address_2 "CSIR-Institute of Microbial Technology" _pdbx_contact_author.address_3 "Chandigarh, India" # loop_ _sas_scan_intensity.id _sas_scan_intensity.momentum_transfer _sas_scan_intensity.intensity _sas_scan_intensity.intensity_su_counting _sas_scan_intensity.scan_id 1 5.548550E-03 4.075220E+01 1.255115E+01 1304 2 5.968380E-03 3.454360E+01 8.757764E+00 1304 3 6.388220E-03 3.319500E+01 5.620842E+00 1304 4 6.808050E-03 2.848200E+01 4.457702E+00 1304 5 7.227880E-03 2.806140E+01 3.480940E+00 1304 6 7.647710E-03 2.882860E+01 3.238216E+00 1304 7 8.067540E-03 2.620450E+01 2.422798E+00 1304 8 8.487370E-03 2.398080E+01 2.333504E+00 1304 9 8.907200E-03 2.171870E+01 2.225624E+00 1304 10 9.327030E-03 2.083770E+01 1.861466E+00 1304 11 9.746860E-03 1.975680E+01 1.762396E+00 1304 12 1.016670E-02 2.031794E+01 1.744962E+00 1304 13 1.058650E-02 1.847676E+01 1.475200E+00 1304 14 1.100630E-02 1.673396E+01 1.354710E+00 1304 15 1.142620E-02 1.531238E+01 1.225222E+00 1304 16 1.184600E-02 1.511660E+01 1.270388E+00 1304 17 1.226580E-02 1.351646E+01 1.132962E+00 1304 18 1.268570E-02 1.367502E+01 1.065092E+00 1304 19 1.310550E-02 1.331674E+01 1.055910E+00 1304 20 1.352530E-02 1.175564E+01 1.002675E+00 1304 21 1.394520E-02 1.141276E+01 9.695196E-01 1304 22 1.436500E-02 1.068316E+01 9.263464E-01 1304 23 1.478480E-02 9.556420E+00 8.232630E-01 1304 24 1.520460E-02 9.540420E+00 8.230730E-01 1304 25 1.562450E-02 9.428340E+00 7.910524E-01 1304 26 1.604430E-02 9.058520E+00 7.689762E-01 1304 27 1.646410E-02 8.302200E+00 7.246870E-01 1304 28 1.688400E-02 8.177860E+00 6.730580E-01 1304 29 1.730380E-02 6.873980E+00 6.487700E-01 1304 30 1.772360E-02 6.782120E+00 5.964748E-01 1304 31 1.814350E-02 6.198500E+00 5.957862E-01 1304 32 1.856330E-02 6.342900E+00 6.042328E-01 1304 33 1.898310E-02 6.374520E+00 5.480658E-01 1304 34 1.940290E-02 5.398560E+00 5.627958E-01 1304 35 1.982280E-02 5.715700E+00 5.216810E-01 1304 36 2.024260E-02 5.283160E+00 5.244436E-01 1304 37 2.066240E-02 4.383160E+00 4.967512E-01 1304 38 2.108230E-02 4.954360E+00 5.039438E-01 1304 39 2.150210E-02 4.632700E+00 4.884032E-01 1304 40 2.192190E-02 4.362900E+00 4.790346E-01 1304 41 2.234180E-02 3.923540E+00 4.570184E-01 1304 42 2.276160E-02 3.536700E+00 4.348750E-01 1304 43 2.318140E-02 3.726960E+00 4.392234E-01 1304 44 2.360130E-02 3.392980E+00 4.327126E-01 1304 45 2.402110E-02 3.428300E+00 4.233640E-01 1304 46 2.444090E-02 3.459580E+00 4.358094E-01 1304 47 2.486070E-02 3.403740E+00 3.905660E-01 1304 48 2.528060E-02 2.754340E+00 4.008546E-01 1304 49 2.570040E-02 2.776360E+00 4.006532E-01 1304 50 2.612020E-02 2.850400E+00 4.063782E-01 1304 51 2.654010E-02 2.434440E+00 3.731400E-01 1304 52 2.695990E-02 2.645580E+00 3.699248E-01 1304 53 2.737970E-02 2.135200E+00 3.821160E-01 1304 54 2.779960E-02 2.381420E+00 3.605394E-01 1304 55 2.821940E-02 1.979940E+00 3.659948E-01 1304 56 2.863920E-02 2.191920E+00 3.709238E-01 1304 57 2.905900E-02 2.228660E+00 3.731132E-01 1304 58 2.947890E-02 2.312480E+00 3.780958E-01 1304 59 2.989870E-02 2.117820E+00 3.577880E-01 1304 60 3.031850E-02 1.899320E+00 3.625746E-01 1304 61 3.073840E-02 1.943580E+00 3.662212E-01 1304 62 3.115820E-02 2.236640E+00 3.560650E-01 1304 63 3.157800E-02 1.704000E+00 3.542454E-01 1304 64 3.199790E-02 1.834640E+00 3.460406E-01 1304 65 3.241770E-02 1.683000E+00 3.595446E-01 1304 66 3.283750E-02 1.933140E+00 3.436194E-01 1304 67 3.325730E-02 1.449860E+00 3.355816E-01 1304 68 3.367720E-02 1.441520E+00 3.498970E-01 1304 69 3.409700E-02 1.400980E+00 3.330426E-01 1304 70 3.451680E-02 1.529040E+00 3.536506E-01 1304 71 3.493670E-02 1.743240E+00 3.341226E-01 1304 72 3.535650E-02 1.315160E+00 3.447650E-01 1304 73 3.577630E-02 1.195220E+00 3.370710E-01 1304 74 3.619620E-02 1.348960E+00 3.414468E-01 1304 75 3.661600E-02 1.215200E+00 3.360166E-01 1304 76 3.703580E-02 1.350900E+00 3.223254E-01 1304 77 3.745560E-02 1.536580E+00 3.246950E-01 1304 78 3.787550E-02 1.260220E+00 3.183310E-01 1304 79 3.829530E-02 1.132600E+00 3.139106E-01 1304 80 3.871510E-02 1.224720E+00 3.155088E-01 1304 81 3.913500E-02 1.167980E+00 3.090036E-01 1304 82 3.955480E-02 1.219620E+00 3.099208E-01 1304 83 3.997460E-02 8.842000E-01 2.986146E-01 1304 84 4.039450E-02 9.282800E-01 2.972688E-01 1304 85 4.081430E-02 1.067640E+00 3.036386E-01 1304 86 4.123410E-02 1.071560E+00 2.932308E-01 1304 87 4.165390E-02 8.017800E-01 2.986660E-01 1304 88 4.207380E-02 1.183000E+00 2.884350E-01 1304 89 4.249360E-02 8.724000E-01 2.869828E-01 1304 90 4.291340E-02 9.841198E-01 2.824222E-01 1304 91 4.333330E-02 1.098040E+00 2.767852E-01 1304 92 4.375310E-02 1.028640E+00 2.849904E-01 1304 93 4.417290E-02 9.384000E-01 2.747770E-01 1304 94 4.459280E-02 9.656202E-01 2.630820E-01 1304 95 4.501260E-02 9.415800E-01 2.656250E-01 1304 96 4.543240E-02 7.076404E-01 2.656856E-01 1304 97 4.585220E-02 9.428994E-01 2.660430E-01 1304 98 4.627210E-02 7.762000E-01 2.632064E-01 1304 99 4.669190E-02 6.840006E-01 2.599408E-01 1304 100 4.711170E-02 8.041602E-01 2.678102E-01 1304 101 4.753160E-02 8.562398E-01 2.608524E-01 1304 102 4.795140E-02 6.474194E-01 2.537800E-01 1304 103 4.837120E-02 7.352994E-01 2.525512E-01 1304 104 4.879110E-02 7.828200E-01 2.571596E-01 1304 105 4.921090E-02 6.298202E-01 2.522274E-01 1304 106 4.963070E-02 5.909202E-01 2.511258E-01 1304 107 5.005050E-02 7.694204E-01 2.553272E-01 1304 108 5.047040E-02 7.327606E-01 2.504812E-01 1304 109 5.089020E-02 6.027400E-01 2.490208E-01 1304 110 5.131000E-02 5.458001E-01 2.413128E-01 1304 111 5.172990E-02 6.946198E-01 2.495946E-01 1304 112 5.214970E-02 6.715600E-01 2.424294E-01 1304 113 5.256950E-02 5.837202E-01 2.461786E-01 1304 114 5.298940E-02 5.097999E-01 2.382312E-01 1304 115 5.340920E-02 5.892997E-01 2.409684E-01 1304 116 5.382900E-02 4.091798E-01 2.412210E-01 1304 117 5.424890E-02 6.717798E-01 2.402268E-01 1304 118 5.466870E-02 3.494400E-01 2.340540E-01 1304 119 5.508850E-02 4.466400E-01 2.343884E-01 1304 120 5.550830E-02 5.368010E-01 2.367492E-01 1304 121 5.592820E-02 7.620798E-01 2.383916E-01 1304 122 5.634800E-02 4.788002E-01 2.283846E-01 1304 123 5.676780E-02 2.896800E-01 2.322718E-01 1304 124 5.718770E-02 5.104599E-01 2.274456E-01 1304 125 5.760750E-02 6.184400E-01 2.294794E-01 1304 126 5.802730E-02 5.848200E-01 2.257380E-01 1304 127 5.844720E-02 4.780600E-01 2.265184E-01 1304 128 5.886700E-02 5.942396E-01 2.283706E-01 1304 129 5.928680E-02 5.730598E-01 2.173498E-01 1304 130 5.970660E-02 5.115796E-01 2.227610E-01 1304 131 6.012650E-02 5.886998E-01 2.251686E-01 1304 132 6.054630E-02 4.791003E-01 2.217410E-01 1304 133 6.096610E-02 3.095000E-01 2.160938E-01 1304 134 6.138600E-02 4.472806E-01 2.197372E-01 1304 135 6.180580E-02 5.948200E-01 2.209678E-01 1304 136 6.222560E-02 4.422002E-01 2.179252E-01 1304 137 6.264550E-02 5.335396E-01 2.165934E-01 1304 138 6.306530E-02 4.945598E-01 2.123274E-01 1304 139 6.348510E-02 4.422600E-01 2.126404E-01 1304 140 6.390490E-02 5.090599E-01 2.206828E-01 1304 141 6.432480E-02 5.121400E-01 2.116232E-01 1304 142 6.474460E-02 4.398007E-01 2.140368E-01 1304 143 6.516440E-02 3.692600E-01 2.094054E-01 1304 144 6.558430E-02 4.126400E-01 2.077678E-01 1304 145 6.600410E-02 3.775799E-01 2.090162E-01 1304 146 6.642390E-02 4.034800E-01 2.079014E-01 1304 147 6.684380E-02 3.740400E-01 2.121070E-01 1304 148 6.726360E-02 3.710606E-01 2.099904E-01 1304 149 6.768340E-02 3.521200E-01 2.084136E-01 1304 150 6.810320E-02 5.295204E-01 2.102856E-01 1304 151 6.852310E-02 3.244002E-01 2.046848E-01 1304 152 6.894290E-02 3.927998E-01 2.078742E-01 1304 153 6.936270E-02 4.554802E-01 2.046204E-01 1304 154 6.978260E-02 4.401206E-01 2.054408E-01 1304 155 7.020240E-02 3.110001E-01 2.041632E-01 1304 156 7.062220E-02 2.918002E-01 1.972702E-01 1304 157 7.104210E-02 4.610196E-01 2.044874E-01 1304 158 7.146190E-02 2.766800E-01 1.961514E-01 1304 159 7.188170E-02 3.854596E-01 2.005406E-01 1304 160 7.230160E-02 4.055400E-01 1.948768E-01 1304 161 7.272140E-02 3.985598E-01 1.981010E-01 1304 162 7.314120E-02 2.806201E-01 1.967982E-01 1304 163 7.356100E-02 3.752004E-01 1.945500E-01 1304 164 7.398090E-02 4.404798E-01 1.990530E-01 1304 165 7.440070E-02 3.149206E-01 1.940792E-01 1304 166 7.482050E-02 3.289406E-01 1.953902E-01 1304 167 7.524040E-02 3.533800E-01 1.945292E-01 1304 168 7.566020E-02 1.846595E-01 1.940038E-01 1304 169 7.608000E-02 3.478600E-01 1.983978E-01 1304 170 7.649980E-02 3.587600E-01 1.951228E-01 1304 171 7.691970E-02 2.261201E-01 1.972732E-01 1304 172 7.733950E-02 3.167203E-01 1.934282E-01 1304 173 7.775930E-02 4.346204E-01 1.918572E-01 1304 174 7.817920E-02 2.136000E-01 1.918956E-01 1304 175 7.859900E-02 3.370204E-01 1.899336E-01 1304 176 7.901880E-02 2.807195E-01 1.939316E-01 1304 177 7.943870E-02 3.081798E-01 1.897592E-01 1304 178 7.985850E-02 4.042999E-01 1.921320E-01 1304 179 8.027830E-02 2.062006E-01 1.889000E-01 1304 180 8.069810E-02 2.341195E-01 1.885516E-01 1304 181 8.111800E-02 2.103604E-01 1.917042E-01 1304 182 8.153780E-02 2.936394E-01 1.857368E-01 1304 183 8.195760E-02 2.429200E-01 1.904332E-01 1304 184 8.237750E-02 2.643799E-01 1.850236E-01 1304 185 8.279730E-02 2.023400E-01 1.894678E-01 1304 186 8.321710E-02 2.032403E-01 1.890322E-01 1304 187 8.363700E-02 2.863595E-01 1.930898E-01 1304 188 8.405680E-02 2.377799E-01 1.950966E-01 1304 189 8.447660E-02 1.387599E-01 1.993372E-01 1304 190 8.489650E-02 2.232799E-01 1.974894E-01 1304 191 8.531630E-02 2.706197E-01 2.024334E-01 1304 192 8.573610E-02 2.440206E-01 1.983072E-01 1304 193 8.615590E-02 3.956600E-01 2.046840E-01 1304 194 8.657580E-02 4.085004E-01 2.007542E-01 1304 195 8.699560E-02 2.072604E-01 1.976786E-01 1304 196 8.741540E-02 1.095000E-01 2.014386E-01 1304 197 8.783530E-02 3.309000E-01 2.008006E-01 1304 198 8.825510E-02 2.081797E-01 2.016894E-01 1304 199 8.867490E-02 3.673400E-01 2.097516E-01 1304 200 8.909480E-02 1.128599E-01 2.045854E-01 1304 201 8.951460E-02 2.364000E-01 2.048756E-01 1304 202 8.993440E-02 2.597996E-01 2.032734E-01 1304 203 9.035420E-02 3.320604E-01 2.061300E-01 1304 204 9.077410E-02 2.389796E-01 2.048406E-01 1304 205 9.119390E-02 2.666401E-01 2.042280E-01 1304 206 9.161370E-02 2.307000E-01 2.028210E-01 1304 207 9.203360E-02 1.201599E-01 2.063922E-01 1304 208 9.245340E-02 2.307407E-01 2.055498E-01 1304 209 9.287320E-02 8.042040E-02 2.062828E-01 1304 210 9.329310E-02 1.187597E-01 2.087496E-01 1304 211 9.371290E-02 3.045198E-01 2.184246E-01 1304 212 9.413270E-02 3.635998E-01 2.035196E-01 1304 213 9.455250E-02 2.098597E-01 2.077026E-01 1304 214 9.497240E-02 1.172001E-01 2.021542E-01 1304 215 9.539220E-02 3.242599E-01 2.038808E-01 1304 216 9.581200E-02 3.217000E-01 2.016854E-01 1304 217 9.623190E-02 2.014200E-01 2.022634E-01 1304 218 9.665170E-02 1.553202E-01 2.000644E-01 1304 219 9.707150E-02 1.808199E-01 2.004424E-01 1304 220 9.749140E-02 2.261598E-01 2.015438E-01 1304 221 9.791120E-02 1.344203E-01 2.013240E-01 1304 222 9.833100E-02 2.348200E-01 2.023734E-01 1304 223 9.875080E-02 1.013204E-01 1.969180E-01 1304 224 9.917070E-02 2.029002E-01 1.965656E-01 1304 225 9.959050E-02 7.086000E-02 1.957960E-01 1304 226 1.000100E-01 1.519000E-01 1.917040E-01 1304 227 1.004300E-01 1.813801E-01 2.027252E-01 1304 228 1.008500E-01 1.992599E-01 1.922390E-01 1304 229 1.012700E-01 2.842201E-01 1.973430E-01 1304 230 1.016900E-01 3.676200E-01 1.948938E-01 1304 231 1.021090E-01 2.662805E-01 1.911050E-01 1304 232 1.025290E-01 1.314393E-01 1.925600E-01 1304 233 1.029490E-01 1.938798E-01 1.935328E-01 1304 234 1.033690E-01 2.834200E-01 1.936278E-01 1304 235 1.037890E-01 1.269993E-01 1.896912E-01 1304 236 1.042090E-01 3.245194E-01 1.908586E-01 1304 237 1.046280E-01 2.076802E-01 1.899690E-01 1304 238 1.050480E-01 1.297402E-01 1.906630E-01 1304 239 1.054680E-01 1.220200E-01 1.936110E-01 1304 240 1.058880E-01 2.338400E-01 1.891002E-01 1304 241 1.063080E-01 1.607206E-01 1.899034E-01 1304 242 1.067280E-01 9.064028E-02 1.898336E-01 1304 243 1.071470E-01 1.876203E-01 1.899666E-01 1304 244 1.075670E-01 2.015003E-01 1.903570E-01 1304 245 1.079870E-01 7.211974E-02 1.845144E-01 1304 246 1.084070E-01 -1.385976E-02 1.855262E-01 1304 247 1.088270E-01 1.223396E-01 1.863774E-01 1304 248 1.092470E-01 1.269400E-01 1.883066E-01 1304 249 1.096660E-01 4.603952E-02 1.868508E-01 1304 250 1.100860E-01 1.656998E-01 1.868394E-01 1304 251 1.105060E-01 9.839620E-03 1.852382E-01 1304 252 1.109260E-01 1.576999E-01 1.842934E-01 1304 253 1.113460E-01 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