Models:
The structure of monomer is not known. The structure of the trimer is taken as the best fitting rigid body model from a previous study (SASDB94). The structure of the monomer is approximated here by taking 1 protomer from the trimeric rigid body model.


Analysis:
Scattering profiles for the monomer and trimer were calculated from the models described above using CRYSOL. CRYSOL intensities were normalised by a factor of re^2 (cm^2 e^-2)  NA (mol^-1)  10^-3 (L cm^-3) = 4.78181  10^-5 (c^m-1 e^2 mol^-1 L) such that when they are multiplied by a molar concentration, they are directly comparable to scattering data on an absolute scale, Fitting was performed in Excel using the "Solver" package (Note that in the Excel document, there is an additional factor of 10^-6, so that all concentrations have units of uM). A linear combination of each of these curves was fit to the scattering data, but the relative concentrations of monomer and trimer were constrained by a global equilibrium constant. For this analysis, 1 equilibrium constants was fit:

1) Ktrimer: 3Monomer <-> Trimer


Files/Directory description:
Models-Crysol		-	PDB files for the monomer, dimer and trimer
			-	Intesnity (.int) and log (.log) files from CRYSOL

PmScsC_XpXmg		- 	Experimental scattering data (.dat) files for PmScsC at each nominal concentration
			-	GNOM output files (.out) for each PmScsC dataset
			-	Fit files (.fit) for the modelling

EquilibriumAnalysis	- 	Excel document in which the fitting was performed
