{
  "code": "SASDXR4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
    "publication": {
      "title": "Structural and biophysical insights into RomR, MglB and MglC interactions involved in regulating cell polarity in Myxococcus xanthus",
      "author_list": "Kodesia A, Kapoor S, Thakur K",
      "journal": "Journal of Biological Chemistry",
      "doi": "10.1016/j.jbc.2025.110907",
      "pmid": null,
      "published_date": "2025 Nov"
    },
    "status": "released",
    "submitted_date": "2025-05-18",
    "released_date": "2025-11-07"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDXR4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXR4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXR4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXR4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDXR4_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXR4_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXR4.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Two-component system response regulator",
          "short_name": "RomR(328-421)",
          "sequence": "MDHHHHHHSSGVDLGTENLYFQSASAAAQPSISIEDSLPDQGDAEEISLDIATPAPVAARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQH",
          "organism": "Myxococcus xanthus",
          "uniprot_code": "A0AAE6G1Y1",
          "uniprot_range_first": 328,
          "uniprot_range_last": 421,
          "oligomerization": "trimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPKNLLVADDSLTIRKVIGMIFATEDFQVTAVDNGLDAISRTRELRPDVVLADVMMPGKS\nGYEVCEALKNDPATQGIPVVLLAGTFEAFDENRARAARADDHVTKPFESQVLLDKVKALV\nGQKSNTMPASAATQVRHAAPQPVAAPAPVAAAAPPGARPAPPPGARPGVPPGPGVPRPPP\nGAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMARPPGPGVPPGA\nRPPGPGMPPGARPGVPPPPGGSAPGLPPRPGMPPGAVARPGVPPPPGGPAPGGFSRPPVG\nAPQPPPGAAPPPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQGDAEEISLDIATPAPVA\nARPASARAPAADGGEALLREALSKASREVIEKIAWEVVPQLAETIIREELERLIKDRETQ\nH",
          "mw": 12.89,
          "total_mw": 38.67,
          "number_molecules": 3,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct contains an additional 25 non-native amino acids at the N-terminus, including a polyhistidine tag (MDHHHHHHSSGVDLGTENLYFQSAS)."
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 150 mM NaCl, 10% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C-terminal region of RomR protein from Myxococcus xanthus",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "CSIR",
            "address": "Chandigarh, India",
            "full_name": "CSIR-Institute of Microbial Technology",
            "webpage": "http://www.imtech.res.in/"
          }
        ],
        "contributor_name": "Akriti",
        "contributor_surname": "Kodesia",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-03-31",
    "storage_temperature": -20.0,
    "cell_temperature": 4.0,
    "exposure_time": 2.0,
    "number_of_frames": 750,
    "wavelength": 0.0991,
    "sample_detector_distance": 2.81,
    "concentration_min": null,
    "concentration_max": 1.0,
    "sample_volume": 50.0,
    "flow_rate": 0.6,
    "s_min": 0.063,
    "s_max": 5.21,
    "total_exposure_time": null,
    "seccolumn": 34
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 58.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 48.0,
  "porod_mw_error": null,
  "pddf_i0": 47.81,
  "pddf_i0_error": null,
  "guinier_i0": 47.3745,
  "guinier_i0_error": null,
  "pddf_rg": 4.283,
  "pddf_rg_error": null,
  "guinier_rg": 4.127,
  "guinier_rg_error": 0.024,
  "pddf_dmax": 16.65,
  "pddf_dmax_error": null,
  "porod_volume": 81.2,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 36,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXR4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-11-07T14:21:25.980490+01:00",
  "bragg_peak": []
}