data_SASDCR9_MAIN # _sas_result.id 760 _sas_result.SASBDB_code SASDCR9 _sas_result.experimental_MW 258.500 _sas_result.experimental_MW_error . _sas_result.MW_standard . _sas_result.MW_standard_error . _sas_result.MW_Porod 279.700 _sas_result.MW_Porod_error . _sas_result.I0_from_PR 0.0298000000 _sas_result.I0_from_PR_error 0.0002900000 _sas_result.I0_from_Guinier 0.0294900000 _sas_result.I0_from_Guinier_error 0.0002000000 _sas_result.Rg_from_PR 5.600 _sas_result.Rg_from_PR_error 0.030 _sas_result.Rg_from_Guinier 5.410 _sas_result.Rg_from_Guinier_error 0.050 _sas_result.D_max 19.50 _sas_result.Porod_volume 374.00 _sas_result.Porod_volume_error . _sas_result.estimated_volume . _sas_result.estimated_volume_error . _sas_result.estimated_volume_method . _sas_result.Guinier_point_min 4 _sas_result.Guinier_point_max 26 _sas_result.type_of_curve sec _sas_result.standard . _sas_result.Dmax_error . _sas_result.comments "For the SEC-SAXS analysis, a mixture of Kif2A, tubulin and DARPin was passed continuously through an X-ray sample cell via an in-line size exclusion column (GE Superdex 200 10/300 GL) at a flow rate of 1 ml/min. The column was pre-equilibrated with running buffer consisting of 20 mM HEPES, 150 mM NaCl, 1 mM MgCl2 pH 7.2. The protein sample was prepared at 25 μM tubulin, 27 μM DARP in and 20 μM Kif2A and injected into a 100 μL loop. Approximately 1200 two-second exposures were collected per sample, and 100 buffer profiles preceding the elution peaks were averaged and used for background subtraction." # _sas_scan.title "Kif2A-tubulin-DARPin complex in presence of AMP-PNP" _sas_scan.detc_id 12 _sas_scan.id 760 _sas_scan.measurement_date 2016-05-16 _sas_scan.storage_temperature 4.0 _sas_scan.cell_temperature 22.0 _sas_scan.exposure_time 2.000 _sas_scan.number_of_frames 1200 _sas_scan.unit 1/A _sas_scan.qmin 0.0086 _sas_scan.qmax 0.2735 _sas_scan.sample_id 720 _sas_scan.result_id 760 _sas_scan.beam_id 23 # _sas_beam.id 23 _sas_beam.instrument_name "Cornell High Energy Synchrotron Source (CHESS) G1" _sas_beam.instrument_city "Ithaca, NY" _sas_beam.instrument_country USA _sas_beam.shape . _sas_beam.type_of_source "X-ray synchrotron" _sas_beam.radiation_wavelength 0.126700 _sas_beam.dist_spec_to_detc 1.47000 # _sas_detc.id 12 _sas_detc.name "Finger Lakes CCD" _sas_detc.type CCD _sas_detc.pixsize_x . # _sas_sample.id 720 _sas_sample.name "Kif2A-tubulin-DARPin complex in presence of AMP-PNP" _sas_sample.ext_coefficient . _sas_sample.contrast . _sas_sample.specific_vol . _sas_sample.purity_method . _sas_sample.dry_vol . _sas_sample.sample_MW . _sas_sample.specimen_concentration 3.70 _sas_sample.concentration_method . loop_ _sas_sample.entity_id 875 890 902 903 # _sas_buffer.id 435 _sas_buffer.name "HEPES 20 mM, MgCl2 1mM, NaCl 150mM" _sas_buffer.concentration . _sas_buffer.unit . _sas_buffer.pK . _sas_buffer.pH 7.200 _sas_buffer.comment . _sas_buffer.composition . _sas_buffer.sample_id 720 # loop_ _entity.id _entity.type _entity.pdbx_description _entity.formula_weight _entity.pdbx_number_of_molecules 875 protein "Designed Ankyrin Repeat Protein D1" 17.886 1 890 protein "Kinesin-like protein KIF2A" 47.834 1 902 protein "Tubulin alpha-1B chain" 50.151 2 903 protein "Tubulin beta-2B chain" 49.936 2 # loop_ _entity_name_com.entity_id _entity_name_com.name 875 "DARPin D1" 890 Kif2A 902 "alpha tubulin" 903 "beta tubulin" # loop_ _entity_poly.entity_id _entity_poly.pdbx_seq_one_letter_code 875 MGSHHHHHHGSDLGKKLLEAARAGQDDEVRILMANGADVNATDASGLTPLHLAATYGHLEIVEVLLKHGADVNAIDIMGSTPLHLAALIGHLEIVEVLLKHGADVNAVDTWGDTPLHLAAIMGHLEIVEVLLKHGADVNAQDKFGKTAFDISIDNGNEDLAEILQKLN 890 MGSSHHHHHHSSGLVPRGSSRRKSNCVKEVEKLQEKREKRRLQQQELREKRAQDVDATNPNYEIMCMIRDFRGSLDYRPLTTADPIDEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVDLTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRKGKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKEL 902 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY 903 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA # loop_ _entity_src_gen.entity_id _entity_src_gen.gene_src_common_name 875 "synthetic construct" 890 "Homo sapiens" 902 "Bos taurus" 903 "Bos taurus" # loop_ _struct_ref.id _struct_ref.entity_id _struct_ref.db_name _struct_ref.db_code _struct_ref.details 1 875 UniProt . http://www.uniprot.org/uniprot/O00139 2 890 UniProt O00139 http://www.uniprot.org/uniprot/P81947 3 902 UniProt P81947 http://www.uniprot.org/uniprot/Q6B856